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Citations for Cell Signaling Technology :
951 - 1000 of 1039 citations for Ephrin type A receptor 4 EphA4 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Sections were then incubated in rabbit polyclonal anti-αSMA or rabbit monoclonal anti-Runx2 at 4° C overnight (Abcam ab5694, 1:100; Cell Signaling Technology, 1:100). Slides were thoroughly washed the next day then incubated in goat anti-rabbit IgG Alexa Fluor 647 secondary antibody for 1 hour at room temperature (ThermoFisher A-21245 ...
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bioRxiv - Neuroscience 2020Quote: ... membranes were probed overnight at 4°C with Rabbit anti-KHSRP (1:1000; Novus) or Rabbit anti-GAPDH (1:2000; Cell Signaling Tech, Beverly, MA), Streptavidin-HRP (1:10000 ...
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bioRxiv - Neuroscience 2020Quote: ... The sections were incubated overnight at 4°C in the primary antibodies against c-Fos (1:6400; catalog #2250S; Cell Signaling Technology, Danvers, MA), and then incubated with the secondary Alexa Fluor® 448 (1:800 ...
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bioRxiv - Cell Biology 2021Quote: ... 50μg of chromatin were immunoprecipitated overnight at 4°C with an antibody against Rev-erb-α (13418S, Cell signaling Technology, Danvers, Massachusetts, United States). BSA/yeast tRNA-blocked Protein A/G dynabeads (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: ... PFA-fixed sections were incubated with the following primary antibodies overnight at 4°C: rabbit anti-cleaved caspase 3 (Cell signaling, #9661, 1:100), mouse anti-BrdU (Dako ...
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bioRxiv - Neuroscience 2020Quote: ... Primary antibodies were diluted with blocking solution and incubated with cells overnight at 4°C: Cleaved caspase-3 (rabbit, Cell Signaling #9661, 1:500), Anti-Nestin (mouse ...
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bioRxiv - Neuroscience 2020Quote: ... Membranes were then rinsed and blocked with 5% BSA for 1hr prior to overnight incubation (4°C) with monoclonal rabbit anti-Phospho-p38 MAPK (Thr180/Tyr182) (D3F9)-XP (Cell signaling, 4511T, 1:1,000) or monoclonal rabbit anti-p38 MAPK (D13E1 ...
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bioRxiv - Neuroscience 2020Quote: ... and incubated for 48 hr at 4°C with antibodies against phosphorylated S6 ribosomal protein (Ser240/244, 1:800, Cell Signaling Technology, catalog # 5364S), GFP (1:1000 ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... and incubation with the primary antibodies in the blocking buffer for overnight at 4°C was followed by a two-hour incubation with secondary antibodies (Cell Signaling, Danvers, MA, USA) in the blocking buffer at room temperature ...
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bioRxiv - Molecular Biology 2019Quote: ... Tween for 1 h and detection was carried out overnight at 4 °C with rabbit anti-phospho-Chk1 (Ser345) mAb (133D3) (Cell Signaling 2348; Lot 15) or rabbit anti-Phospho-Chk2 (Thr68 ...
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bioRxiv - Molecular Biology 2020Quote: ... and subsequently incubated overnight at 4 °C with the primary anti-ADPr-antibody (diluted 1:10’000 in TBS-T; Cell Signaling Technology, Leiden, Netherlands25, 26). Membranes were washed 3x for 5 min with TBS-T and incubated IRDye goat-anti-rabbit IgG secondary antibody (diluted 1:15’000 in TBS-T ...
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bioRxiv - Developmental Biology 2019Quote: ... Blots were probed overnight at 4 deg C with rabbit anti-V5 (Cell Signaling Technologies; 1:1000 in 5% BSA, 0.1% Tween 20, TBS) or for 1 hr at room temperature with mouse anti-β actin (GeneTex ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were separated on a 4–12% SDS-PAGE gel followed by standard immunoblot analysis using anti–CISH (Cell Signaling, Clone D4C10, 1:2000) and Vinculin (Cell Signaling ...
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bioRxiv - Neuroscience 2021Quote: ... The membranes were incubated at 4°C overnight with the following mouse-specific primary antibodies: anti-NLRP3 (D4D8T, rabbit monoclonal antibody, 1:1000, #15101, Cell Signaling Technology, Danvers, MA), anti-cleaved Caspase 1 (p20 ...
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bioRxiv - Neuroscience 2021Quote: ... The blots were stained with Ponceau S solution and blocked with 5% nonfat milk in PBS/0.05% Tween 20 for 1 h at RT and subsequently incubated at 4 °C overnight with the following primary antibodies: rabbit anti-MBP (1:1000; Cell Signaling, Danvers, MA, USA), mouse anti-CRYAB (1:1000 ...
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bioRxiv - Cell Biology 2022Quote: ... 500 μg of lysates were incubated overnight at 4 °C on a rotating wheel with an IgG Isotype Control antibody (Cell Signaling Technology, 1:300), or anti-IRF1 antibody (Cell Signaling Technology ...
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bioRxiv - Cell Biology 2022Quote: ... 300 μg of lysates were incubated overnight at 4 °C on a rotating wheel with an IgG Isotype Control (Cell Signaling Technology, 1:300), anti-Ollas (Novus ...
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bioRxiv - Biochemistry 2022Quote: ... Membranes were blocked in 5% milk and incubated overnight at 4 °C in primary antibody (anti-PPARγ, Santa Cruz #sc-7273; or anti-β-actin, Cell Signaling #3700). Washes were performed in TBS-T (20 mM Tris ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were subsequently incubated overnight at 4° C with primary anti-phosphorylated ERK antibody (anti-pERK, Cell Signaling, Cat # 4370, RRID: AB_2315112, 1:400). After primary antibody incubation and PBS wash ...
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bioRxiv - Cancer Biology 2023Quote: ... Slides were then stained with the appropriate primary antibody diluted in blocking solution and incubated overnight at 4°C: Phospho-Histone H3 (Cell Signaling, #9701, 1:200), Cleaved Caspase 3 (Cell Signaling ...
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bioRxiv - Neuroscience 2022Quote: ... sections were incubated at 4°C overnight in rabbit anti-c-fos(1:1000 dilution, 2250T Rabbit mAb, Cell Signaling Technolog, Danvers, Massachusetts, USA) primary antibody or mouse anti-TPH2 (1:100 dilution ...
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bioRxiv - Developmental Biology 2023Quote: ... The cells were then incubated overnight at 4°C with a variety of primary antibodies including anti-MFN2 (Cell Signaling Technology; diluted 1:200), anti-TRA-1-81 (diluted 1:200 ...
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bioRxiv - Cell Biology 2024Quote: ... Slides were then incubated overnight with the following primary antibodies at 4°C: rabbit anti-Ki67 (1/200; 12202; Cell Signaling Technology, Danvers, MA), rabbit anti-Olfm4 (1/400 ...
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bioRxiv - Microbiology 2024Quote: ... Lysates were then precleared by incubation for 1 hour at 4°C while rotating with 50 μL protein A agarose beads (Cell Signaling Technologies, Cat #9863) CCNT1 antibody (Cell Signaling Technologies ...
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bioRxiv - Immunology 2023Quote: ... at room temperature for 1 hour and then incubated overnight at 4°C with primary anti–SYK (1:1000 dilution, Cell Signaling Technology, no. 2712S), anti–p-p38 (1:1000 dilution ...
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bioRxiv - Microbiology 2023Quote: ... secondary antibody staining was performed for 10 minutes at 4°C with Anti-Rabbit IgG conjugated to Alexa Fluor® 488 fluorescent dye (Cell Signaling Technology, 4412S). Finally ...
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were blocked in 5% non-fat milk in TBST for 1 h and incubated with the appropriate primary antibody overnight at 4°C: anti-NANOG (D73G4) (Cell Signaling #4903; 1:1000), anti-FAK (D2R2E ...
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bioRxiv - Immunology 2023Quote: ... The blocked membrane was cut and then incubated with shaking overnight at 4°C with either CD19 antibody #3574 (Cell Signaling; 1:1000 dilution) or GAPDH-HRP Conjugate (Cell Signaling ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were then incubated overnight at 4°C in the following primary antibodies: rabbit anti-cleaved NOTCH1 (Val 1744) at 1:1000 (Cell signaling technology, Cat #: 4147S), rabbit anti-NOTCH1 (D1E11 ...
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bioRxiv - Developmental Biology 2023Quote: ... and the samples were incubated with the following primary antibodies overnight at 4°C: Rabbit anti-p44/42 MAPK (Erk1/2) (1:1000, 4695, Cell Signaling Technology, MA, USA), rabbit anti-phospho-p44/42 MAPK (Erk1/2 ...
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bioRxiv - Cell Biology 2023Quote: ... and an equivalent of 1 µg of DNA-normalized DPCs was resolved using SDS-acrylamide gradient gel with the addition of 4 M urea (Cell Signaling, #38650, 1: 1000). DNA detection was performed by applying 2 ng of the sample collected for Pico Green detection to a nylon membrane and detecting with the α-dsDNA antibody (abcam ab27156 ...
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bioRxiv - Microbiology 2023Quote: ... and the remaining volume was rotated overnight at 4 °C with 45 μl of anti-C-myc magnetic beads from Cell Signaling Technology (Cat. 5698) or Pierce (Cat ...
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bioRxiv - Genomics 2023Quote: ... membranes were incubated overnight at 4°C with different primary antibodies in TBS-T against LEF-1 (1:500, Antibody# 2230, Cell Signaling Technology, MA, USA), and β-actin (1:30000 ...
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bioRxiv - Genetics 2023Quote: ... 0.1% Tween 20 and 5% BSA overnight at 4 °C with anti-Runx2 antibody (RUNX2 D1H7 Rabbit mAb, Cell Signaling Technology, 84866 1:2000) and anti-β-actin antibody (Rabbit Anti-beta Actin ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were then incubated with 200 μl of 1XDPBS + 1% BSA + 0.1% Tween-20 containing 4 μl of Alexa Fluor 488-conjugated phospho-histone H3 (Ser10) antibody (Cell Signaling Technologies, 1:50 dilution) for 90 minutes at room temperature ...
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bioRxiv - Immunology 2023Quote: ... The blocked membrane was cut and then incubated with shaking overnight at 4°C with either CD19 antibody #3574 (Cell Signaling; 1:1000 dilution), EAAT3 antibody (Cell Signaling ...
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bioRxiv - Developmental Biology 2023Quote: ... embryos were incubated overnight at 4°C with the primary antibodies (Mettl3 Abcam ab195352 1/200; N-Myc Cell Signaling Technology D1V2A 1/200). EU fluorescence coupling was performed following the manufacturer ’s instructions for Click-iT™ RNA Alexa Fluor 488 Imaging Kit ...
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bioRxiv - Biochemistry 2023Quote: ... Coverslips were then blocked with 5% BSA for 60 minutes at room temperature and incubated overnight at 4°C with primary antibody γH2AX (rabbit monoclonal; Cell Signaling 9718T, 1:400). Texas red species-specific secondary antibodies (Jackson ImmunoResearch Laboratories Inc. ...
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bioRxiv - Neuroscience 2023Quote: ... the membrane was blocked in 5% nonfat milk in PBST (PBS and 0.1% TWEEN20) and incubated overnight at 4°C with primary antibodies mouse anti-GFP Living Color (Cell Signaling Technology 2955, 1:1000) to detect TDP-43 YFP and rabbit anti-Beta-Actin (Cell Signaling Technology 4967S ...
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bioRxiv - Cell Biology 2023Quote: ... Embryos were incubated for 1 hr in blocking buffer (PBS-Triton, FBS 10%) then overnight at 4°C with anti-Yap antibody (Cell Signaling, #4912, 1:200) in blocking buffer ...
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bioRxiv - Physiology 2023Quote: ... Neurons were then incubated overnight at 4°C in 1% bovine serum albumin in PBS with pCREB (Ser133) (87G3) Rabbit mAb (1:333, Cell Signaling, Cat No. #9198). Neurons were then thrice rinsed with PBS ...
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bioRxiv - Neuroscience 2023Quote: ... was incubated overnight at 4°C followed by corresponding horseradish peroxidase (HRP)-linked anti-mouse (Cell Signaling Technology, 7076V, 1:2000 dilution in TBST) or anti-rabbit (Cell Signaling Technology ...
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bioRxiv - Microbiology 2023Quote: ... followed by blocking in milk and further overnight probing at 4 °C with a primary antibody specific for Cre (1:500; Cell Signaling Technology, mAb #12830) or GAPDH (1:5000 ...
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Shear stress and very low levels of ligand synergize to activate ALK1 signaling in endothelial cellsbioRxiv - Cell Biology 2023Quote: ... Cells were incubated overnight at 4° C with rabbit anti-pSMAD1/5/9 (1:600 dilution, Cat: 13820, Cell Signaling Technology, Danvers, MA USA). The secondary antibodies ...
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bioRxiv - Immunology 2023Quote: ... and incubated overnight at 4°C with mild agitation in 5% milk in TBST plus indicated antibody: cytochrome c (1:750, rabbit, Cell Signaling Technology, Cat. 11940), GAPDH (1:10000 ...
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bioRxiv - Cell Biology 2024Quote: ... 500 μg of lysates were incubated overnight at 4 °C on a rotating wheel with an anti-HA antibody (Cell Signaling Technology, 1:100). The next day ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The tissues were then incubated overnight at 4°C with anti-DeltaFosB primary antibody (DeltaFOSB; rabbit; Cell Signaling Technology, ref. 22509; 1:1000 dilution) or anti-Fos antibody (c-Fos ...
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bioRxiv - Cell Biology 2024Quote: ... The supernatants (Triton X-100 insoluble fraction) were collected and analyzed on 4-20% SDS-PAGE with anti-ubiquitin (Cell Signaling Technologies P4D1, #3936) and anti-Ref(2)P/p62 (Abcam #178840 ...
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bioRxiv - Cell Biology 2024Quote: ... The membrane was incubated at 4°C overnight with the following primary antibodies: anti-phospho-MEK1/2 (Cell Signaling Technology, Inc., MA, USA, #9122), anti-MEK1/2 (Santa Cruz biotechnology ...
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bioRxiv - Neuroscience 2024Quote: ... samples were incubated for 48 hours at 4°C in PBS-T-containing rabbit anti-IGF1R β antibody (1:1000; Cell signaling, Cat#9750), which has been previously validated [46] ...