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Citations for Cell Signaling Technology :
801 - 850 of 1039 citations for Ephrin type A receptor 4 EphA4 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... for 1 hour and probed overnight at 4°C with the primary antibodies of interest directed against: rabbit anti-BCL-2 (Cell Signaling, CST4223), rabbit anti-BCL-xL (Cell Signaling ...
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bioRxiv - Genetics 2021Quote: ... were incubated overnight at 4 degrees Celcius in a 1:1000 dilution with the following primary antibodies: anti-TP53 (Cell Signaling, 2524), anti-BRCA1 (Abclonal ...
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bioRxiv - Genetics 2021Quote: ... The following primary antibodies were used at 1:1000 dilution in PBST (0.05% Tween-20) and 5% non-fat dry milk and incubated overnight at 4°C: Myc (Cell Signaling Technology #2276), RBPJ (Cell Signaling Technology #5313) ...
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bioRxiv - Developmental Biology 2021Quote: ... cells were fixed in 4% paraformaldehyde and processed for immunostaining by standard methods using mouse anti-Flag (9A3, Cell Signaling, 1:400), rabbit anti-Myc (71D10 ...
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bioRxiv - Molecular Biology 2021Quote: ... and Tween20 (0.02%)) for 20 minutes at RT followed by treatment overnight at 4 °C with anti-γ-H2AX (Cell Signaling #9718S) diluted 1:500 in Ab mix or anti-PCNA diluted 1:1000 (DAKO #M0879) ...
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bioRxiv - Developmental Biology 2021Quote: ... 0.3% Triton X-100), incubated in rabbit anti-phospho-Histone H3-S10 at 1:2000 overnight (O/N, 4°C) (Cell Signaling 3377S), washed 8X in PBSTx (1X PBS plus 0.3% Triton X-100 ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were incubated overnight in blocking buffer at 4°C with the following primary antibodies: 1:2000 c-fos (Cell signaling technology), 1:2000 ds-red (Takara Bio) ...
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bioRxiv - Developmental Biology 2019Quote: ... Embryos/explants were then blocked in PBS+ 10% FBS and 1% Triton X-100 and incubated overnight at 4°C with an anti-pSmad2/3 antibody (Cell Signaling #8828) at 1:1000 in block ...
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bioRxiv - Neuroscience 2019Quote: ... They were then incubated at 4°C overnight with the following primary antibodies diluted in buffer G: rabbit polyclonal anti-cleaved caspase-3 (Asp175) (Cell Signaling, #9661) at 1:250 ...
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bioRxiv - Cancer Biology 2020Quote: ... Blots were incubated with the primary antibodies in TBS 0.05% Tween-20 / 5% milk overnight at 4°C and subsequently incubated with HRP coupled secondary goat anti-rabbit (7074; Cell Signaling, Switzerland) and goat anti-mouse antibody (7076 ...
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bioRxiv - Molecular Biology 2019Quote: ... Membranes were blocked in 5% milk/PBS-T buffer for 30 min and incubated either overnight at 4°C or for 1 hour at room temperature with the following antibodies: Chd1 (Cell Signaling, 4351), Top2β (Santa Cruz Biotechnology ...
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bioRxiv - Genetics 2020Quote: ... followed by overnight incubation with primary antibodies overnight at 4°C (anti-dS6K gift from Thomas Neufeld; dilution at 1:3500, anti-pS6KThr398 Cell Signaling Technologies, #9209 ...
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bioRxiv - Cancer Biology 2020Quote: ... cells grown on chamber microscope slides were fixed in 4% paraformaldehyde and treated with immunofluorescence blocking buffer (catalog #12411, Cell Signaling Technology). Staining with primary antibodies against involucrin (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2020Quote: ... blocked using 5% milk in TBST for 1 hour followed by primary antibody incubation at 4°C for overnight using the following antibodies at the appropriate dilutions– LC3 (1:1000, #2775, Cell Signaling Technologies), Atg5 (1:1000 ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were incubated with 100 µl/well of diluted H3K9ac on a shaker at 4°C overnight and then treated with Alexa Fluor 488 conjugate secondary antibody (Cell Signaling #4412) at room temperature ...
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bioRxiv - Physiology 2022Quote: ... antibody in 0.1% TBST containing 3% BSA overnight at +4°C followed by incubation with secondary HRP-linked anti-rabbit antibody (Cell Signaling, #7074). Immunoblotted proteins were detected with ProSignal Pico ECL Reagent (Genesee Scientific ...
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bioRxiv - Cancer Biology 2022Quote: ... run using standard Ventana reagents a and Vector secondaries for staining with the TrkB.T1 SPEH1_D12 scFv-Fc fusion (Pattwell et al. 2020b) at 1:500 and PTEN (Cell Signaling #9188, Lot #4) at 1:100 or TrkB (kinase specific against amino acid 810 ...
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bioRxiv - Cell Biology 2022Quote: ... The membranes were incubated with 1:5 home-made anti-p53 (overnight at +4°C) and 1:100,000 anti-Lamin B1 (1 hr at room temperature) (Cell Signaling Technology, #12586). As secondary antibodies ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were then incubated overnight at 4 °C with primary antibodies diluted in blocking buffer: anti-myosin-5a antibody (1:100, #3402, Cell Signaling Technology), or anti-myosin-6 antibody (1:200 ...
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bioRxiv - Cancer Biology 2021Quote: ... followed by an overnight incubation at 4°C with the following primary antibodies: 4EBP1 (1:1,000, Cell Signaling Technology, Cambridge, UK #9644), MYCN (1:1,000 ...
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bioRxiv - Biochemistry 2020Quote: ... The membranes were then incubated overnight at 4 °C with either rabbit anti-p-MEK antibody against Ser217/Ser221 (1:1000) (Cell Signaling, #9121), rabbit anti-p-ERK antibody against Thr202/Tyr204 (1:1000 ...
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bioRxiv - Genomics 2020Quote: ... The samples were blocked in 10% goat serum and incubated overnight at 4°C with the mouse Cas9 antibody (7A9-3A3 Cell Signaling Technologies) 1:800 dilution in 1% BSA (Supp ...
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bioRxiv - Cell Biology 2020Quote: ... for 1 h at room temperature followed by incubation overnight at 4°C with a specific primary antibody to p-p53ser15 (Cell Signaling, 9284S), β-actin (Cell Signaling ...
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bioRxiv - Cell Biology 2019Quote: ... Filters were incubated at 4 °C with primary antiserum diluted in 5% milk TBST as follows: FLAG antiserum (1:1000, Cell Signaling Technology), α-tubulin antiserum (1 ...
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bioRxiv - Cell Biology 2019Quote: ... Samples were then incubated overnight on a plate rocker at 4° C in a 1:200 dilution of anti-phospho-Erk1/2 (Thr202/Tyr204) rabbit monoclonal antibody (Cell Signaling 4370) in blocking buffer ...
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bioRxiv - Genetics 2019Quote: ... Membranes were blocked in 5% non-fat milk and incubated overnight at 4 °C with primary antibodies: anti-GPR151 (LSBio # LS-B6760-50) or anti-beta-actin (Cell Signaling #3700). Subsequently ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were then stained overnight at 4°C with AlexaFluor 647-conjugated anti-β-catenin antibody L54E2 at 1:300 dilution supplemented with 3% BSA (2677S, Cell Signaling). Between and after each of these steps ...
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bioRxiv - Cell Biology 2020Quote: Cells were trypsinized and washed with PBS and then lysed for 30 minutes on a rotating wheel at +4 °C in RIPA buffer supplemented with protease/phosphatase inhibitor cocktail (Cell Signaling, 5872). Lysates were sonicated with a Bioruptor Soni cation System (UCD200 ...
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bioRxiv - Physiology 2021Quote: ... Blots were blocked in 5% bovine serum albumin in tris-buffered saline with 0.1% Tween 20 (TBS-T) and incubated overnight at 4°C with rabbit anti-phospo-Ser505-Akt (Cell Signaling Technology 4054) or mouse anti-α-Tubulin (DSHB 12G10 ...
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bioRxiv - Developmental Biology 2021Quote: ... incubated overnight at 4°C with a 1:1000 dilution of rabbit anti-phosphorylated-SMAD1/5/8 (clone 41D10, Cell Signaling Technologies) in block ...
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bioRxiv - Physiology 2021Quote: ... Sections were blocked with 5% donkey serum in the presence of 0.1% Triton followed by overnight incubation with primary antisera at 4 degrees (pCREB, Cell Signaling; Glucagon, Abcam). After extensive washing ...
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bioRxiv - Cancer Biology 2020Quote: ... The neutrophils were then incubated overnight at 4°C with primary antibodies: anti-histone H3 (1:50 dilution; Cell Signaling Technology, 3680) and either anti-NE (1:100 dilution ...
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bioRxiv - Immunology 2020Quote: ... The cell lysates were incubated overnight at 4 °C with NEMO antibody and ChIP-Grade Protein G Magnetic Beads (Cell Signaling Technology).
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bioRxiv - Microbiology 2021Quote: ... membranes were incubated at 4°C overnight with the following primary antibodies: rabbit anti-ubiquitin (1:1000; Cell Signaling Technology no. 3933), rabbit anti-phospho-eIF2α (1:1000 ...
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bioRxiv - Microbiology 2020Quote: ... incubated overnight at 4°C with the following primary antibodies diluted 1:1000 in 5% BSA/TBST: anti-pro-IL-1β (#12703, Cell Signaling Technology) and anti-NLRP3 (AG-20B-0014-C100 ...
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bioRxiv - Molecular Biology 2021Quote: ... The membranes were blocked with 5% BSA in TBST buffer for 2 h at RT and incubated overnight at 4°C with primary antibodies against ERK1/2 (1:1000; 9102s, Cell Signaling Technology), pERK1/2 (1:1000 ...
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bioRxiv - Cell Biology 2021Quote: ... and 10 µg/mL of aprotinin and leupeptin] and incubated (16 hrs., 4°C) with either 3 µg non-immune control rabbit IgG (Cell Signaling #272) or with 3 µg of ERRα antibody (Cell Signaling #13826 ...
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bioRxiv - Immunology 2022Quote: ... Membranes were blocked for 1 hour at room temperature with blocking buffer (Licor) and incubated overnight at 4°C with anti-K48-linked poly-ubiquitin antibody (Cell Signaling Technologies) and anti-β-actin antibody (Santa Cruz Biotechnologies) ...
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bioRxiv - Developmental Biology 2022Quote: ... blots were incubated overnight at 4°C in the following primary antibodies: β-III-Tubulin (TUJ1, 1:1000; Cell Signaling Technology, 5568), SUFU (1:1000 ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then incubated with primary anti-RAB5 antibody at 4 °C overnight diluted in 8% BSA in PBST (1:500 dilution, Cat. #3547, lot 7, Cell Signaling Technology). Secondary anti-rabbit Alexa Fluor 568 antibody (1:1000 dilution ...
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bioRxiv - Biophysics 2022Quote: ... 15 μg of total protein was loaded onto an SDS-PAGE (4-12%) to assess LRP6 and β-catenin protein levels using phospho- and total LRP6 (Cell Signaling), active- and total β-catenin (Cell Signaling ...
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bioRxiv - Bioengineering 2022Quote: ... the membrane was incubated overnight at 4°C or with primary antibody solution (1:1000 dilution for anti-Alix [E4T7U, Cell Signaling Technology] ...
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bioRxiv - Cancer Biology 2022Quote: ... followed by 20% swine serum in PBS and then incubated with primary antibodies overnight at 4 °C (γH2AX ((#9718, Cell Signaling Technologies). The following day ...
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bioRxiv - Developmental Biology 2022Quote: ... Magnetic beads were allowed to bind with 5 μg of antibody (Histone H3 Lysine 4 trimethylation; H3K4me3; Cell Signaling Technology, cat. #9751) by incubating for 8 hours at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... for 1h at room temperature and incubated over night at 4°C with the following antibodies diluted in blocking solution: anti-RSK2 (1/1000, Cell Signaling Technology); anti-RPS6 (1/1000 ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were incubated with primary antibodies diluted in blocking buffer over night at 4°C (SHIP1, 1:500 dilution, Cell Signaling Technologies; IBA1 ...
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bioRxiv - Immunology 2024Quote: ... then stained at 4°C overnight with various combinations of the following antibodies: rabbit anti-NeuN (1:1000, Cell Signaling Technologies, 12943S), rabbit anti-Iba1 (1:1000 ...
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bioRxiv - Neuroscience 2023Quote: ... for 1 hour and then incubated overnight at 4°C with anti-p-AKT (Ser473) antibody (1:1000 dilution, RRID: AB_2315049, Cell Signaling Technology 4060T) and anti-H3 antibody (internal control ...
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bioRxiv - Cancer Biology 2024Quote: ... 4×106 cells were fixed in methanol and incubated with anti-pS6 (Ser235/236) rabbit antibody (1:100, Cell Signaling Technology #2211), followed by incubation with cell surface markers and secondary Alexa Fluor 647 goat anti-rabbit (1:200 ...
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bioRxiv - Neuroscience 2024Quote: ... sheared chromatin was centrifuged at 16,000 x g for 20 minutes at 4 °C and dissolved in 1x ChIP buffer (Cell Signaling Technology, #57976). 2% input was taken and chromatin was incubated with antibodies overnight at 4°C ...