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Citations for Cell Signaling Technology :
51 - 100 of 744 citations for Nuclear Protein Extraction since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2019Quote: ... Immunocaptured proteins were isolated using Protein-G agarose beads (Cell Signaling Technology), washed multiple times with CHAPS buffer ...
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bioRxiv - Immunology 2020Quote: ... Protein concentration was determined by BCA protein assay kit from Cell Signaling Technology. Thirty micrograms of total protein were mixed in a reducing sample buffer and used for mitochondrial apoptosis assay per the kit instruction ...
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bioRxiv - Cancer Biology 2021Quote: ... protein lysis buffer (Cell Signaling) containing a protease inhibitor cocktail was added and the mixture homogenized ...
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bioRxiv - Cell Biology 2023Quote: ... we used either HRP-conjugated protein A or HRP-conjugated protein G (both from Cell Signaling) because of high background from the immunoglobulin bands when we used secondary antibodies ...
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bioRxiv - Microbiology 2020Quote: ... S6 Ribosomal protein (5G10, Cell Signaling), BIM (C34C5 ...
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bioRxiv - Immunology 2019Quote: ... Protein A agarose beads (Cell signaling) were used for IgG pulldown ...
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bioRxiv - Physiology 2022Quote: ... and Biotinylated Protein ladder (Cell Signaling) were included on the gels ...
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bioRxiv - Molecular Biology 2022Quote: ... protein lysis buffer (Cell Signaling Technology) containing a protease inhibitor cocktail was added ...
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bioRxiv - Cell Biology 2023Quote: ... one for each protein (Cell Signaling). Each membrane piece was then incubated overnight at 4 °C ...
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bioRxiv - Immunology 2019Quote: ... Quantified protein (between 200-500 µg) was incubated with protein-specific or isotype control antibody (Cell Signaling Technology) in lysis buffer at 4°C overnight with head over tail rotation ...
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bioRxiv - Immunology 2020Quote: ... HA-tagged proteins were immunoprecipitated with anti-HA and ChIP-Grade Protein G Magnetic Beads (Cell Signaling Technology). Flag-tagged proteins were immunoprecipitated with Anti-FLAG M2 Magnetic Beads (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins bound to Protein-A Dynabeads were pulled down using a magnetic separation rack (Cell Signaling, no. 14654). After 5 washes in NP-40 lysis buffer ...
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bioRxiv - Immunology 2023Quote: The protein concentration was adjusted to 30 μg by adding protein lysis buffer (Cell Signaling Technology, Danvers, MA) and 4x loading buffer (Bio-Rad ...
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bioRxiv - Cell Biology 2024Quote: ... 1 mg of total protein was pre-cleared by Pierce Protein A/G Magnetic Beads (Cell Signaling Technology) for 1 hour at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: Protein A magnetic beads (Cell Signaling Technology) were pre-washed with cell lysis buffer twice ...
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bioRxiv - Developmental Biology 2021Quote: ... containing a Protein A slurry (Cell Signaling). A non-specific antiserum (control IgG ...
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bioRxiv - Cancer Biology 2022Quote: ... S6 ribosomal protein (2217; Cell Signaling Technology), Akt1 (2938 ...
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bioRxiv - Immunology 2021Quote: ... coupled to Protein G beads (Cell Signaling) overnight with rotation at 4°C ...
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bioRxiv - Developmental Biology 2021Quote: ... containing a Protein A slurry (Cell Signaling). A non-specific antiserum (control IgG ...
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bioRxiv - Cancer Biology 2022Quote: ... protein A/G agarose beads (Cell Signaling) were added and allowed to rotate for another 2h ...
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bioRxiv - Immunology 2020Quote: ... The same amount of protein was immunoprecipitated with anti-NEMO and ChIP-Grade Protein G Magnetic Beads (Cell Signaling Technology). The IKKα/β kinase activity in the IP complex was tested by using IKKβ Kinase Enzyme Kit (Promega ...
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bioRxiv - Biochemistry 2024Quote: ... that combines the IgG binding domain of both Protein A and Protein G were used to cross-link anti-Kv11.1 antibodies (Cell Signaling). Stock beads with a concentration of 10 mg/ml were washed four times with 10 volumes of Dulbecco’s Phosphate Buffered Saline (DPBS ...
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bioRxiv - Biochemistry 2022Quote: Protein A agarose beads (Cell Signaling Technologies 9863S) were washed and incubated in NET150 buffer (20 mM Tris HCl ph ...
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bioRxiv - Developmental Biology 2022Quote: ... S6 Ribosomal Protein (1:1000, Cell Signaling #2217), LEF1 (1:250 ...
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bioRxiv - Cell Biology 2022Quote: ... ribosomal protein S6 (#2217 CST) from Cell Signaling. Mouse monoclonal anti-beta Actin AC-15 HRP (#AB49900 ...
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bioRxiv - Molecular Biology 2021Quote: ... or directed to T6B-fusion protein (Cell Signaling anti-FLAG #8146S ...
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bioRxiv - Cell Biology 2022Quote: ... 30 μl Protein G agarose beads (Cell Signaling) were washed in IP buffer and blocked with bovine serum albumin (BSA) ...
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bioRxiv - Biochemistry 2019Quote: ... When blotting for total proteins (Cell Signaling Technology; EGFR #4267 ...
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bioRxiv - Cell Biology 2020Quote: ... or Protein A HRP conjugate (Cell Signaling Technology) diluted at 1:5000 in blocking buffer for 45 min at room temperature ...
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bioRxiv - Cancer Biology 2020Quote: ... S6 ribosomal protein (Cell Signaling 2217, 1:1000), phospho-S1859 CAD (Cell Signaling 70307 ...
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bioRxiv - Cell Biology 2021Quote: ... S6 ribosomal protein mouse mAB (2317S, Cell Signaling), phospho-p70 S6 kinase (Thr389 ...
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bioRxiv - Cancer Biology 2022Quote: ... Protein A/G agarose beads (Cell Signaling Technology) were then added and incubated for 2 hr at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... S6 ribosomal protein (1:1000; 54D2; Cell Signaling), BIM (1:1000 ...
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bioRxiv - Cancer Biology 2024Quote: ... S6 Ribosomal protein (Cell Signaling Technologies, Cat. # 2217S), p4EBP1 (S65 ...
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were separated by SDS–polyacrylamide gel electrophoresis and the proteins were detected with the following antibodies: LOXL2 (Cell Signaling; 99680S), BRD4 (Abcam ...
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bioRxiv - Microbiology 2023Quote: ... SARS-CoV-2 spike protein-specific IgG was measured using SARS-CoV2 spike protein serological IgG ELISA kit (Cell Signaling Technology) per manufacturer’s instructions.
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bioRxiv - Microbiology 2024Quote: ... The following antibodies were used for viral protein analysis: SARS-CoV-2 anti-Spike and anti-N protein (cat #569996 and #33336, respectively, Cell Signaling) and anti-β-actin (Sigma – cat #a2228) ...
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bioRxiv - Neuroscience 2021Quote: ... Total protein concentration was determined by a BCA assay (Pierce) 60 μl Protein A beads with 50% slurry (Cell Signaling Technology, #9863S) were then added and incubated for 6 hours to pull down the antibody-protein complex ...
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bioRxiv - Neuroscience 2022Quote: ... or DIV12 neurons in the presence of recombinant protein (see recombinant protein below) or C1q-blocking antibody (Recombinant Anti-C1q antibody (ab182451)) / IgG (Normal Rabbit IgG,Cell Signaling Technology). For LPS induced activation of microglia ...
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bioRxiv - Molecular Biology 2023Quote: ... and myc- and HA-tagged proteins were immunoprecipitated with Protein A/G beads (GeneBio, 22202B-1) coated with anti-myc antibody (Cell signaling, 2276S) at a dilution of 1:250 or anti-HA antibody (Cell signaling ...
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MIIP downregulation promotes colorectal cancer progression via inducing adjacent adipocytes browningbioRxiv - Cancer Biology 2023Quote: ... Protein lysates containing 1 mg total protein were precleaned and precipitated with indicated antibody or IgG (Cell Signaling Technology, Danvers, MA, US) for 6 h at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... The resulting mixtures of protein-DNA and antibody were further incubated with ChIP-Grade Protein G Magnetic Beads (9006, Cell Signaling Technology). After sequential washing with low- and high-salt solutions ...
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bioRxiv - Developmental Biology 2020Quote: ... Total protein was extracted with RIPA buffer (Cell Signaling) with cOmplete protease inhibitor (Roche ...
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bioRxiv - Physiology 2019Quote: ... mTOR and the reference protein β-actin (Cell Signaling). Protein quantification was determined using fluorescence ...
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bioRxiv - Cell Biology 2022Quote: ... ChIP-Grade protein G magnetic beads (Cell Signaling, 9006S), 25 μl per IP ...
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bioRxiv - Microbiology 2020Quote: ... P-6S Ribosomal protein (Ser235/236, D57.2.2E Cell Signaling), S6 Ribosomal protein (5G10 ...
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bioRxiv - Neuroscience 2022Quote: ... PhosphoSitePlus: Protein Modification Site (Cell Signaling Technology; RRID: SCR_001837), Image J (RRID ...
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bioRxiv - Microbiology 2019Quote: ... rabbit anti-protein disulfide-isomerase (PDI, Cell Signaling C81H6) for labeling ER was used at 1:200 ...
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bioRxiv - Physiology 2019Quote: ... phospho-S6 ribosomal protein (1:1000; #5364; Cell Signaling), and histone H3 protein (1:1000 ...
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bioRxiv - Cell Biology 2020Quote: ... 20 µl of Protein A agarose beads (Cell Signaling) were added and incubation continued for 3-5 hours at 4°C ...