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Citations for Cell Signaling Technology :
1 - 50 of 681 citations for Nuclear Protein Extraction since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Cells were lysed in 500 μl of nuclear extraction buffer A (Cell Signaling Technology, Cat. 7006) on ice for 15 min and washed once with 500 μl of nuclear extraction buffer B (Cell Signaling Technology ...
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bioRxiv - Systems Biology 2022Quote: ... Immunoblot analysis was used to assess the adequacy of nuclear purification by measuring Lamin A/C (rabbit, 1:1000, Cell signaling # 2032, a nuclear protein).
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bioRxiv - Microbiology 2020Quote: ... on ice for 15 min and washed once with 500 μl of nuclear extraction buffer B (Cell Signaling Technology, Cat. 7007), and then treated with 0.5 μL of micrococcal nuclease (Cell Signaling Technology ...
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bioRxiv - Cell Biology 2019Quote: ... and protein extraction was carried out using RIPA buffer (Cell Signaling Technology, U.S.A.) supplemented with protease and phosphatase inhibitor cocktails (Roche ...
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bioRxiv - Cancer Biology 2021Quote: ... 500 μg of nuclear protein lysates were incubated with 2 μg of either Myc-Tag antibody (Cell Signaling Technology) or an endogenous NUP210-specific antibody (Bethyl Laboratories) ...
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bioRxiv - Neuroscience 2021Quote: ... Samples from nuclear fractions were incubated with antibodies for nuclear loading control CREB (1:2000, Cell Signaling). Following primary antibody incubation ...
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bioRxiv - Neuroscience 2022Quote: ... The antibodies used on membranes containing cytosolic and nuclear protein fractions were: rabbit anti PCBP1 (1:1000, #8534, Cell Signaling), mouse anti PCNA (1:1000 ...
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bioRxiv - Cancer Biology 2022Quote: ... DRAQ5 nuclear stain (Cell Signaling Technologies, #4084L) was added to the last well at a dilution of 1:100 ...
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bioRxiv - Neuroscience 2022Quote: ... Samples from nuclear fractions were incubated with antibodies for nuclear loading control histone 3 (H3; 1:1000, Cell Signaling). Whole-cell tissue samples were incubated with antibodies for phospho-MAPK (1:1000 ...
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bioRxiv - Microbiology 2021Quote: ... and laminin B1 (nuclear fraction) (Cell signaling Technology). Unintegrated nuclear DNA was isolated using the Hirt DNA isolation method ...
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bioRxiv - Biochemistry 2022Quote: ... for nuclear pluripotency markers OCT4 (Cell Signaling, #2750) and NANOG (Cell Signaling ...
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bioRxiv - Plant Biology 2019Quote: ... Protein extraction and immunoblotting with an antibody against phosphorylated MAPKs (p44/42; 9101; Cell Signaling Technology) was then performed ...
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bioRxiv - Developmental Biology 2019Quote: ... Nuclear staining was by DAPI (1:1000, Cell Signaling). Samples were imaged on a Leica TCS SP5 Scanning Confocal ...
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bioRxiv - Developmental Biology 2019Quote: ... For some experiments the nuclear stain Draq5 (Cell Signaling #4084S) was used instead of an anti-histone antibody.
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bioRxiv - Bioengineering 2021Quote: ... and nuclear fractions to HRP-tagged Histone H3 (#12648, Cell Signaling). All blots were developed using Immobilon Luminata Forte (Sigma ...
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bioRxiv - Developmental Biology 2022Quote: Immunofluorescence used anti–human nuclear KU80 antigen (Cell signaling Technology, C48E7) or anti human mitochondria antibody (anti-CoxIV ...
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bioRxiv - Neuroscience 2023Quote: ... nuclear factor kappa B NF-κB (1:500; #8282, Cell Signaling) and its phosphorylated form (S536 ...
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bioRxiv - Cancer Biology 2023Quote: ... Primary antibodies raised against proliferating cell nuclear antigen (PCNA, Cell Signaling, D3H8P), phosphorylated ERK (Cell Signaling ...
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bioRxiv - Cancer Biology 2021Quote: ... including proliferating cell nuclear antigen (PCNA) (cat#2586S, Cell Signaling Technology, Boston, MA), H2A histone family member X (H2AX)(cat#7631S ...
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bioRxiv - Cell Biology 2019Quote: ... Nuclear counterstaining was performed using 1 μg/ml Hoechst 33342 (no. 4082; Cell Signaling) at room temperature for 15 min in the dark ...
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bioRxiv - Physiology 2023Quote: ... nuclear factor kappa beta (NF-kB) (1:500, Cell Signaling Tech-nologies, Madrid, Spain).
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bioRxiv - Immunology 2023Quote: ... and nuclear fractions per manufacturer’s instructions (Cell Fractionation Kit, Cell Signaling Technology, Danvers, MA). Extracts were then separated by SDS-PAGE ...
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bioRxiv - Neuroscience 2020Quote: ... ProLong Gold Antifade Reagent with DAPI was used for nuclear staining (Cell Signaling Technology, #8961S). Stains were visualized in the High Speed Widefield confocal (UCSF Nikon core ...
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bioRxiv - Physiology 2023Quote: ... CD44 expression and nuclear localization was determined using anti-CD44 (Cell Signaling; Catalogue number: 3570S) and PECAM-1 expression using anti-PECAM-1 (Sigma Aldrich ...
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bioRxiv - Physiology 2023Quote: ... and nuclear factor erythroid 2-related factor 2 (NRF2, #12721, 1:25, Cell Signaling Technology). Antibody binding was identified by incubation with goat anti-rabbit or anti-mouse secondary antibodies (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... Astrocytes were then collected by scraping and the CNMCS Compartmental Protein Extraction Kit (BioChain Cat# K3013010 Hayward, CA) with protease/phosphatase inhibitors (PI, Cell Signaling, Cat# 5872S, Danvers, MA) was used and the nuclear fractions were isolated for each treatment condition ...
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bioRxiv - Molecular Biology 2021Quote: ... The pre-cleared nuclear lysate was incubated with anti-p300 primary antibody (70088, Cell Signaling Technology) overnight at 4°C with rotation to form immunocomplex which was subsequently incubated with the pre-washed magnetic beads for 20 min at room temperature with rotation ...
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bioRxiv - Cell Biology 2021Quote: ... Nuclear envelope (Alexa Fluor®488 Lamin A/C, clone 4C11, #8617, Cell Signaling, 1:200) and DNA damage site (Alexa Fluor®488-γ-H2A.X ...
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bioRxiv - Developmental Biology 2020Quote: ... which were done with SimpleChIP® Sonication Cell and Nuclear Lysis Buffers (Cell Signaling Technology #81804) following manufacturer’s instruction.
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bioRxiv - Cell Biology 2023Quote: ... Following nuclear staining with 4′,6-diamidino-2-phenylindole (DAPI; 4083S, Cell Signaling Technology, MA, USA), larvae were mounted in 80% glycerol between two coverslips and imaged using fluorescence confocal microscopy ...
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bioRxiv - Genetics 2019Quote: ... membrane/organelle and nuclear/cytoskeletal fractions using the Cell Fractionation Kit (Cell Signaling Technology, Danvers, MA, USA) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: Nuclear extracts from HepG2 cells were prepared and incubated with HuR antibody (Cell Signaling Technology, USA, 12582S), anti-flag antibody (Cell Signaling Technology ...
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bioRxiv - Cell Biology 2023Quote: ... Nuclear envelope marker (Alexa Fluor®488 Lamin A/C, clone 4C11, #8617, Cell Signaling, 1:200) was used to examine subnuclear regions ...
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bioRxiv - Genomics 2020Quote: ... 1mg nuclear lysate in 1.5mL RIP Buffer was incubated with 2.5µg rabbit anti-human RBBP7 antibody (Cell Signaling) or rabbit IgG isotype control antibody (I5006 ...
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bioRxiv - Cancer Biology 2024Quote: ... the collected nuclear supernatant was then combined with the IP antibodies of either 1 μg HDAC7 (Cell Signaling) or 4 μg H3.3 (Abcam ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were separated into cytoplasmic and nuclear compartments fractions that were incubated with GFP antibody (cat# 2555, Cell Signaling) which recognizes the YFP protein ...
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bioRxiv - Neuroscience 2022Quote: ... Nuclear staining was carried out by using 4’,6-diamidino-2-phenylindole (DAPI; 4083S, Cell Signaling Technology, MA, USA). Four different sections with no tissue loss were counted for the quantification of cells ...
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bioRxiv - Developmental Biology 2023Quote: ... Nuclear staining was carried out by using 4′,6-diamidino-2-phenylindole (DAPI; 4083S, Cell Signaling Technology, MA, USA). Larvae were imaged using fluorescence confocal microscopy.
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bioRxiv - Cell Biology 2023Quote: ... Post-PLA slides were further processed for immunofluorescent staining against the nuclear membrane marker (Lamin A/C, #8617, Cell Signaling). After washing three times with PBST ...
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bioRxiv - Developmental Biology 2019Quote: ... Immunohistochemistry was performed as described [24] with primary antibodies against PCNA (proliferating cell nuclear antigen) (Cell Signaling, Danvers, MA, USA, #2586) at a concentration of 1:5000 ...
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bioRxiv - Cell Biology 2021Quote: ... The slides post-PLA were further processed for immunofluorescent staining against the nuclear membrane marker (Lamin A/C, #8617, Cell Signaling). The slides were mounted with Duolink® In Situ Mounting Medium with DAPI (#DUO82040 ...
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bioRxiv - Genetics 2021Quote: ... Specific proteins were detected using 1:1000 dilution of Myc-Tag (9B11) mouse monoclonal and proliferating cell nuclear antigen (PCNA, D3H8P) XP Rabbit monoclonal primary antibodies (Cell Signaling Technology). Following primary antibody incubation ...
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bioRxiv - Cancer Biology 2022Quote: ... was used as a nuclear fraction loading control and α-Tubulin as a cytoplasmic fraction loading control (1:1000, cat# 3873; Cell signaling).
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bioRxiv - Cancer Biology 2023Quote: ... cellular and nuclear fractions from HNSCC cell lines and tissues were prepared using the cell fractionation kit purchased from Cell Signaling (#9038) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... Nuclear stain solution was prepared for 4 slides at a time: 20 µLSYTO13 (Nanostring) and 180 µL 1x TBS (Cell Signaling Technologies, 12498S) for a total volume of 200 µL/slide ...
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bioRxiv - Immunology 2020Quote: ... anti-phospho-inhibitory subunit of nuclear factor κB (NF-κB) (IκB) kinase α (IKKα) (1:1□000 dilution, C84E11, Cell Signaling Technology, USA), anti-total-IKKα (1:1□000 dilution ...
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bioRxiv - Systems Biology 2023Quote: ... the nuclear extract was pre-incubated for 20 minutes on ice with anti-cJUN rabbit mAb (clone 60A8) from Cell Signaling technologies (#9165S) or with anti-IKZF1 (clone IK14) ...
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bioRxiv - Neuroscience 2022Quote: The phospho-dependent association of overexpressed nuclear Jacob was assessed by heterologous co-immunoprecipitation from HEK293T cells pre-treated with 20 nM staurosporine (Cell Signaling Technology, Cat.: #9953S) for 3 h.
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bioRxiv - Physiology 2019Quote: ... Immunocaptured proteins were isolated using Protein-G agarose beads (Cell Signaling Technology), washed multiple times with CHAPS buffer ...
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bioRxiv - Immunology 2020Quote: ... Protein concentration was determined by BCA protein assay kit from Cell Signaling Technology. Thirty micrograms of total protein were mixed in a reducing sample buffer and used for mitochondrial apoptosis assay per the kit instruction ...