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Citations for Cell Signaling Technology :
801 - 850 of 954 citations for Recombinant Human CRP Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2024Quote: ... The synthesized proteins were confirmed by immunoblotting with an anti-FLAG antibody (Fujifilm-Wako) or Streptavidin-HRP (Cell Signaling Technology).
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bioRxiv - Microbiology 2020Quote: ... an amount of digested lysate equivalent to 10 mg of protein was subjected to ubiquitin remnant immunoprecipitation according to the manufacturer’s protocol (Cell Signaling Technologies). For phosphorylation analysis ...
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bioRxiv - Cancer Biology 2021Quote: ... 50 µg total protein was resolved in 8% non-reducing SDS-PAGE gel and blot with PKM2 antibody (Cell Signaling, 3198s).
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bioRxiv - Cell Biology 2020Quote: ... The pull-down assay of ZFP982-MYC tagged protein was performed through co-Immunoprecipitation (co-IP) using MYC-Tag (9B11) antibody (#2276, Cell Signaling). For MYC-co-IP ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were blocked with Q-PBS and probed with antibodies against the following proteins: p-EGFR Y1068 (#3777S, 1:200, Cell Signaling), MET (#AF276 ...
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bioRxiv - Genetics 2021Quote: ... the samples were incubated with 30 ml of ChIP Grade Protein G magnetic beads (Cat# 9006S; Cell Signaling Technology; Danvers, MA) for 2 hours at 4°C under gentle rotation ...
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bioRxiv - Plant Biology 2022Quote: ... The prey and bait proteins were detected using anti-His (1:1000, No. #2365; Cell Signaling Technology [CST], Danvers, MA, USA) and anti-GST (1:1000 ...
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bioRxiv - Molecular Biology 2021Quote: ... Expression of Anti-HA fusion protein was examined by staining cells with rabbit Anti-HA (1:250; #3724, Cell Signaling Technology) and Alexa Fluor@ 488 donkey anti-rabbit IgG (1:2000 ...
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bioRxiv - Molecular Biology 2019Quote: ATM protein expression was assessed by SDS-PAGE and western blot using a rabbit anti-ATM antibody (1:1000; 2873S; Cell Signaling). Horseradish peroxidase-conjugated α-rabbit antibody (1:5000 ...
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bioRxiv - Cancer Biology 2019Quote: ... The resulting phosphorylated RB protein was detected by western blotting using anti-p-Rb (S807/S811) antibody (Cell Signaling Technology, 8516S).
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bioRxiv - Cell Biology 2021Quote: ... Protein accumulation rate upon proteasome inhibition was measured after 3-hour treatment with 10μM of MG-132 (Cell Signaling Technology #2194) in the RPMI medium.
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bioRxiv - Plant Biology 2020Quote: ... and chemiluminescence protein detection was done as described with anti-mouse or anti-rabbit antibodies conjugated with horseradish peroxidase (HRP) from Cell Signaling Corp ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were transferred to nitrocellulose using standard methods and membranes were blocked in 5% non-fat dry milk (Cell Signaling Technology) in TBS with 0.2% Tween-20 (Boston BioProducts) ...
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bioRxiv - Cancer Biology 2021Quote: ... 100ug of protein was incubated with 6 uL of ubiquitin antibody and protease/phosphatase inhibitors (Cell Signaling Technologies, Danvers, MA, USA) overnight ...
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bioRxiv - Biochemistry 2022Quote: Cell lysates for protein analysis were prepared by scraping cells in 1x Cell Lysis Buffer (Cell Signaling Technology, Danvers, MA, USA) containing 1x protease/phosphatase inhibitor (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... For immunoprecipitation assays, cell lysates were incubated with Protein G Agarose (cat # 20398, Pierce) and anti-Flag antibody (cat # 2368, Cell Signaling) at 4°C overnight ...
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bioRxiv - Cell Biology 2022Quote: ... 10-15 μg of proteins were separated by SDS-PAGE and immunoblotted using the following antibodies: p-ERK1/2 (9101, Cell Signaling), ERK1/2 (4695 ...
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bioRxiv - Developmental Biology 2021Quote: ... Quantification of relative densitometry was obtained by normalizing to the background and to loading control proteins (ACTB, from Cell Signaling Technology) using Image Lab Software (Bio-Rad).
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bioRxiv - Microbiology 2020Quote: ... an amount of digested lysate equivalent to 10 mg of protein was subjected to ubiquitin remnant immunoprecipitation according to the manufacturer’s protocol (Cell Signaling Technologies). For phosphorylation analysis ...
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bioRxiv - Molecular Biology 2020Quote: Western blot was performed after chemoenzymatic labeling to detect O-GlcNAcylated proteins using α-streptavidin-HRP (Cell Signaling Technologies, Danvers, MA) (1:3000) ...
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bioRxiv - Plant Biology 2021Quote: ... and the interaction between coexpressed proteins was examined by immunoblot analyses using a mouse anti-Myc monoclonal antibody (1:10,000; Cell Signaling Technology), a rat anti-RFP monoclonal antibody (1:10,000 ...
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bioRxiv - Microbiology 2020Quote: ... Lysed samples were centrifuged and incubated for 3 h with Pierce Protein A/G Magnetic beads (88802) which were pre-incubated over night with V5 antibody (Cell signaling E9H80 ...
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bioRxiv - Cell Biology 2021Quote: ... the membrane was incubated with antibody that recognizes proteins IDH 1 (1:1000) and IDH 2 (1:1000) (Cell Signaling, MA) and α-Tubulin (Abcam ...
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bioRxiv - Plant Biology 2019Quote: ... SDS-PAGE gels and the interaction between co-expressed proteins determined by immunoblot analysis using a mouse anti-MYC monoclonal antibody (1:10,000; Cell Signaling Technology) and a mouse anti-GFP monoclonal antibody (1:5,000 ...
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bioRxiv - Cell Biology 2021Quote: ... The protein A agarose bead was added with 450 µl of IP buffer and 5 µl of Myc antibody (Cell Signaling). The mixture was then incubated on rotator at 4°C for 4 h ...
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bioRxiv - Molecular Biology 2021Quote: ... Individual ChIP experiments were performed on pre-cleared chromatin using antibody-coupled ChIP grade Protein G magnetic beads (Cell Signaling Technology). POLR3A antibody was obtained from Abcam (ab96328 lot#GR318563) ...
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bioRxiv - Microbiology 2020Quote: ... FLAG-tagged and myc-tagged proteins were detected with rabbit anti-DYKDDDDK (FLAG) monoclonal antibody and rabbit anti-myc monoclonal antibody (Cell signaling), respectively ...
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bioRxiv - Biochemistry 2021Quote: ... Primary antibody solution mix contains two primary antibodies raised in two different species (mouse and rabbit) targeting the proteins of interest: rabbit anti-mitofusin 2 (1:50, mAb D2D10, Cell Signaling), rabbit anti-VDAC (1:200 ...
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bioRxiv - Neuroscience 2021Quote: Antibodies for detection of cell surface proteins by western blot of cell lysates were used as provided and were from the following sources: anti-ErbB3 (Cell Signaling, HER3/ErbB3 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... MCF7 cell lysate as prepared above was diluted to 0.8 mg/mL total protein in 1:3 lysis buffer:dilution buffer with 0.5 nM rabbit anti-BRD4 antibody (Cell Signaling Technology; E2A7X) and 1 nM CoraFluor-1-labeled anti-rabbit nano secondary ...
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bioRxiv - Cancer Biology 2020Quote: ... Protein levels were detected using antibodies against Intracellular Domain of Notch1 (ICN) (Val1744 Cell Signaling Technology #4147, D3B8, 1:500 dilution) and β-actin (Millipore-Sigma ...
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bioRxiv - Cancer Biology 2022Quote: ... The separated proteins on the gel were transferred onto PVDF membrane and were probed for specific antibodies against Rb (cat# 9309; Cell signaling) phospho-Rb (cat# 8516 ...
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bioRxiv - Cell Biology 2022Quote: ... protein blots were incubated with the following secondary antibodies Anti-Mouse IgG HRP Linked Antibody (Cell Signaling, Cat # 7076S, 1:2500) and Anti-Rabbit IgG HRP Linked Antibody (Cell Signaling ...
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bioRxiv - Genetics 2022Quote: ... Membranes were blocked in 4% BSA in 1X TBS and probed with S6 ribosomal protein 1:1000 (2217, Cell Signaling Technologies), phosphor-S6 ribosomal protein 1:1000 (2215 ...
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bioRxiv - Microbiology 2022Quote: ... The cells were harvested at 48 h pt and equal amounts of proteins were analyzed by western blot for p-eIF2α or total eIF2α (both antibodies were obtained from Cell Signaling). Expression of E was detected using an HA-tag antibody (Invitrogen) ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were transferred to nitrocellulose membranes at 100 volts for 90 minutes in cold 1X transfer buffer (Cell Signaling Technology, 12539S). Membranes were blocked with 5% (w/v ...
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bioRxiv - Microbiology 2023Quote: ... ADP-ribosylated proteins were visualised by chemiluminescence using the SignalFire ECL Reagent or the SignalFire Elite ECL Reagent (Cell Signaling Technology) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... proteins were transferred to nitrocellulose membrane (Cytiva) and revealed using following antibodies: rabbit AGR2 (Cell Signaling Technologies, Clone D9V2F, 1/1000), mouse FLAG-M2-HRP (Sigma ...
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bioRxiv - Microbiology 2023Quote: ... Protein accumulation in the purified fractions was confirmed with western blot using anti-MBP tag (8G1) mouse monoclonal antibody (Cell Signaling) according to the manufacturer’s instructions.
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bioRxiv - Animal Behavior and Cognition 2023Quote: Primary antibodies/concentrations used were as follows: rabbit anti-phospho-S6 ribosomal protein (Serine240/244) (1:100, Cell Signaling Technologies, #2215), rabbit anti-phospho-S6 ribosomal protein (Serine244/247 ...
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bioRxiv - Cell Biology 2023Quote: Primary antibodies against following proteins were used: MYPT1 (rabbit IgG mAb clone D6C1, #8574) from Cell Signaling Technology (Leiden, The Netherlands), MYPT1 (mouse IgG1 mAb clone C-6 ...
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bioRxiv - Microbiology 2023Quote: Samples used for measuring HMGB1 protein levels in the stool and colonic mucus were homogenized in cell lysis buffer (Cell Signaling) containing complete protease inhibitor (Roche ...
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bioRxiv - Neuroscience 2024Quote: ... every 8th section of the brain was processed for double-fluorescent immunostaining for Ku80 and one of the following: Microtubule-associated protein 2 (MAP2; 8707, Cell Signaling), doublecortin (ab18723 ...
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bioRxiv - Cell Biology 2020Quote: ... The supernatants were collected and incubated with a 30 μl bed volume of protein-A-sepharose beads and 1.5 μl of α-IGF-1 receptor β (D23H3) XP (Cell Signaling) per sample ...
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bioRxiv - Molecular Biology 2019Quote: 500 μg of unstimulated HT29 lysate was immunoprecipitated onto Protein A/G coated wells (Pierce #15138) following the kinase assay protocol but with 0.5 μg of IKKα (Cell Signaling Technology #2682) or 0.5 μg of naïve rabbit IgG (Jackson Immunoresearch #011-000-002) ...
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bioRxiv - Immunology 2021Quote: ... Western blotting was used as the output assay to determine levels of the ribosomal protein S6 kinase (p-S6K) using an S6K and p-S6K antibodies (Cell Signaling Technology). To assess the impact of mTOR1 inhibition rapamycin (20ng/mL ...
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bioRxiv - Microbiology 2020Quote: ... 5 mg of tissue from distal ileal mucosal scrapings was lysed in 250 μL of ice-cold protein lysis buffer (Cell Signaling Technology Cell Lysis Buffer ...
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bioRxiv - Bioengineering 2021Quote: ... Protein level could also be measured by immunofluorescence using fluorescently labeled antibodies (anti-Flag-Alexa 647, #15009; Cell Signaling, Danvers, MA, USA). These antibodies were flowed into the device and incubated with the immobilized proteins under the ‘button’ for 20 min at RT ...
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bioRxiv - Plant Biology 2021Quote: ... The phosphorylated MAPK3 and MAPK6 proteins were detected using polyclonal anti-phospho-p44/42 MAPK (Erk1/2, 1:1000; Cell Signaling Technology), and the detection of the total MAPK3 and 6 protein levels were by using polyclonal anti-MAPK3 (1:1000 ...
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bioRxiv - Cancer Biology 2020Quote: ... Ten to 50 μg of proteins were separated by 10 % SDS-PAGE and visualized with primary Slug or E-cadherin antibody (C19G7, Cell Signaling Technology, #9585 ...