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Citations for Cell Signaling Technology :
751 - 800 of 954 citations for Recombinant Human CRP Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... The lysate was centrifuged at 12 000 g for 15 min and the supernatant with 0.25 mg total protein was incubated with 0.5 μg anti-Ire1α (Cell Signaling) antibody for 4 hours at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... The levels of HuR protein were detected using western blot and anti-HuR antibody (Cell Signaling Technology, USA, 12582S). The probes used are listed in the Supplementary Table 6.
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were transferred to nitrocellulose membranes (Prometheus) and probed with phospho-ROS1 Y2274 (3078; 1:1,000; Cell Signaling Technology), ROS1 (D4D6 ...
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bioRxiv - Cell Biology 2024Quote: ... proteins were transferred to a 0.2um nitrocellulose membrane and stained with primary antibodies including BMI1 (6964S, Cell Signaling Technology), H4 (13919 ...
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bioRxiv - Neuroscience 2024Quote: ... Equal protein loading of blots was confirmed by immunoblotting of GAPDH (rabbit monoclonal, #2118, Cell Signaling Technology, Danvers, USA), except β-tubulin (mouse monoclonal ...
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bioRxiv - Genomics 2024Quote: ... nuclei was cleared by centrifugation and subjected to pre-clearance by incubating with Protein A agarose beads (Cell Signaling). Pre-cleared cell lysates were separately incubated with CTCF or non-specific IgG antibodies (Cell Signaling ...
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bioRxiv - Microbiology 2023Quote: ... The extent of protein knockdown was determined by immunoblotting with an anti-c-Met antibody (#8198, Cell Signaling Technology), anti-E-cadherin antibody (#3195 ...
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bioRxiv - Microbiology 2022Quote: ... For protein detection the following primary antibodies were used at the indicated dilutions: AKT (Cell Signaling, #9272, 1:1000), phospho-AKT T308 (Cell Signaling ...
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bioRxiv - Plant Biology 2023Quote: ... Proteins were transferred to supported nitrocellulose membrane and probed with p44/42 MAPK antibody from Cell Signaling Technology (CST) (9102 ...
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bioRxiv - Developmental Biology 2023Quote: ... 2 μg of protein were added to pre-washed 50 μL streptavidin Sepharose bead conjugates (Cell Signaling Technology, 3419S) and rocked overnight at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... Primary antibodies to the following proteins were used at the indicated concentrations: GABARAP/Atg8a (1:1,000, E1J4E, Cell Signaling), Arl8 (1:1000 ...
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bioRxiv - Cancer Biology 2023Quote: ... proteins were transferred on a PVDF membrane and incubated with the following antibodies: p-ERK (#9101, Cell Signaling Technology), total ERK (#9102 ...
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bioRxiv - Pathology 2024Quote: ... antibodies against the following proteins were used to probe membranes: p-SMAD2 (0.1 µg/mL, 3108S, Cell Signaling Technology), SMAD2 (0.1 µg/mL ...
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bioRxiv - Neuroscience 2024Quote: ... Protein lysates (1 mg) underwent overnight incubation with 10 µg of either PAK1 primary antibody (#2602, Cell Signaling Technology) or Rabbit IgG isotype control (#2729 ...
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bioRxiv - Neuroscience 2024Quote: ... The following primary antibodies were used: rabbit anti-Phospho-S6 Ribosomal Protein (Ser240/244) (1:2000, #5364 Cell Signaling), rabbit anti-S6 Ribosomal Protein (1:2000 #2217 Cell Signaling) ...
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bioRxiv - Molecular Biology 2024Quote: Immune complexes were collected by the addition of 80 μL of protein A-agarose beads (Cell Signaling Technology; 9863) with an additional 2 hour incubation at 4°C with mixing ...
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bioRxiv - Bioengineering 2020Quote: ... Membranes were blocked with 5% skim milk in TBST for 1 h at room temperature and incubated with anti-human p53 antibody (Cell Signaling Technologies, #9282, rabbit polyclonal, 1:1000) in TBST containing 5% skim milk (4°C ...
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bioRxiv - Genetics 2021Quote: ... and Total EIF2α protein levels with rabbit anti-EIF2a polyclonal antibody (1:10,000 dilution) (Cell Signaling Technologies, cat. number D7D3). Additional blots were probed with rat anti-MRP5 NC3 monoclonal antibody (1:200 ...
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bioRxiv - Cell Biology 2022Quote: Primary antibodies used are listed here: Rabbit anti-Phospho-S6 Ribosomal Protein (Ser235/236) (1:1000, Cell Signaling, cat. #2211), Rabbit S6 Ribosomal Protein (5G10 ...
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bioRxiv - Neuroscience 2019Quote: ... We used an antibody for phosphorylated ribosomes (Phospho-S6 Ribosomal Protein [Ser235/236] Antibody #2211, Cell Signaling, Danvers, MA, USA) as a proxy of neural activation [18] ...
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bioRxiv - Physiology 2019Quote: ... Western blotting was performed as previously described (10) and samples were analyzed for protein abundance of FoxO1 (Cell Signaling, #2880), 4-HNE (Abcam ...
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bioRxiv - Molecular Biology 2019Quote: ... Immunoblotting of immunoprecipitated proteins was performed as described13 with the following primary antibodies: IKKα (1:1000, Cell Signaling Technology #2682) and IKKβ (1:1000 ...
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bioRxiv - Systems Biology 2019Quote: ... — 10 mg of digested proteins were dissolved in 1X immunoprecipitation buffer (50 mM MOPS, 10 mM Na2HPO4, 50 mM NaCl, pH 7.2, Cell Signaling). Modified symmetric dimethyl arginine peptides ...
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bioRxiv - Cancer Biology 2021Quote: ... and 10 µg of the protein extracts were resolved by SDS-PAGE and probed using antibodies against STAT3 (1:1,000, #9139S; Cell Signaling), Phospho-STAT3 (1:2,000 ...
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bioRxiv - Neuroscience 2022Quote: ... The antibodies used on membranes containing cytosolic and nuclear protein fractions were: rabbit anti PCBP1 (1:1000, #8534, Cell Signaling), mouse anti PCNA (1:1000 ...
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bioRxiv - Cancer Biology 2022Quote: ... Antibodies against the following proteins were used in the indicated dilutions: ERK1/2 (#4695, RRID:AB_390779, Cell Signaling Technology, 1:50), JUN (#9165 ...
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bioRxiv - Plant Biology 2019Quote: ... Detection of phosphorylated proteins was performed in blocking solution (5% BSA, 0.1% [v/v] Tween-20) with anti-phosphothreonine antibodies (9381, Cell Signaling) and with anti-rabbit-HRP (W4011 ...
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bioRxiv - Cell Biology 2020Quote: ... 2 μg of fusion proteins and 8 μg of FLAG-PKD1-CA were incubated in kinase buffer (Cell Signaling Technology) for 30 min at 30°C in the presence of 200 μM ATP ...
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bioRxiv - Plant Biology 2021Quote: ... 6xHis-tagged ALI1 and 6xHis-tagged control protein were incubated with streptavidin beads (Sepharose® Bead Conjugate, Cell Signaling Technology) that were already loaded with M4 (oxo-C14-HSL tagged with biotin ...
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bioRxiv - Physiology 2021Quote: ... anti-phospho and total p38 mitogen-activated protein kinase antibody (p/total p38 1:1000 Cell Signaling Technology, Danvers, MA). After incubation ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were then transferred onto PVDF membranes and probed with the following antibodies: HIF-1α (D2U3T) (#14179, Cell Signaling Technology), BiP (C50B12 ...
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bioRxiv - Biochemistry 2022Quote: ... Rabbit anti β-actin antibody at 1:1000 dilution was employed for normalization of total protein amount (8457, Cell Signaling). For revelation ...
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bioRxiv - Plant Biology 2022Quote: ... and probed with anti-phospho-p44/42 mitogen-activated protein kinase (Erk1/2) (Thr202/Tyr204) (Cell Signaling; 1:1,000 dilution), anti-MBP (New England Biolab ...
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bioRxiv - Cell Biology 2022Quote: ... Lysates of equal protein concentration were incubated overnight in 4 °C with an anti-Src antibody (Cell Signaling Technology, #2108). Complexes were pulled out using protein-A sepharose beads (GE-Healthcare) ...
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bioRxiv - Molecular Biology 2022Quote: ... The detection of proteins of interest was performed using the following primary antibodies: anti-Ago1 (D84G10, Cell Signaling Technology, 5053), anti-Ago2 (C34C6 ...
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bioRxiv - Cell Biology 2022Quote: ... manufacturer and catalog number shown in brackets): Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E, Alexa Fluor 555, 1:200, Cell Signaling, 39855); S6 Ribosomal Protein (54D2 ...
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bioRxiv - Developmental Biology 2023Quote: ... the following antibodies were used: rabbit anti-CCAAT/enhancer binding protein alpha (C/EBPA, 1:500, 8178P, Cell Signaling Technology), chicken anti-green fluorescent protein (GFP ...
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bioRxiv - Genetics 2023Quote: ... Protein was extracted from frozen tissue by homogenization of tissue with RIPA lysis buffer and PMSF (Cell Signaling, Danvers, MA). Protein concentrations of samples were determined using Bradford Assay (Bio-Rad ...
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Targeting adipocyte ESRRA promotes osteogenesis and vascular formation in adipocyte-rich bone marrowbioRxiv - Molecular Biology 2023Quote: ... Chromatin complexes were immunoprecipitated with primary antibody as below and incubated with protein G magnetic beads (Cell Signaling Technology #9006) overnight ...
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bioRxiv - Microbiology 2023Quote: ... HA- and EGFP-tagged proteins were incubated with anti-HA antibody (1: 2,000; 2999, Cell Signaling Technology, Danvers, MA, USA) or anti-EGFP antibody (1 ...
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bioRxiv - Biochemistry 2023Quote: ... and a rabbit antibody recognizing a cellular marker protein (anti-TGN46, Abcam, ab50595, 1:600; anti-EEA1, Cell Signaling Technology, 2411 ...
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bioRxiv - Biochemistry 2023Quote: ... and blots (20-50 µg of total protein) were probed with the following antibodies: HA (3724S, Cell Signaling; 1:1500), BRCA1 (SC6954 ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell extract were analyzed after migration of 50µg protein extract by Western blotting using a primary antibody against HES1 (#11988, Cell Signaling). Signals were revealed using a rabbit peroxidase-conjugated secondary antibody (1/5000 ...
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bioRxiv - Cancer Biology 2023Quote: ... Up to 30 µg of protein lysate was separated by SDS-PAGE and transferred to nitrocellulose membranes before antibody incubation (IκBα: Cell Signaling, CS9242 ...
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bioRxiv - Neuroscience 2023Quote: ... anti-Phospho-S6 ribosomal protein (Ser240/244) (pS6) (rabbit polyclonal, #2215, RRID: AB_331682, Cell Signaling Technology; 1:1000 for WB), anti-S6 ribosomal protein (S6 ...
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bioRxiv - Cancer Biology 2023Quote: ... samples were subjected to enrichment of tyrosine phosphorylated proteins using an the PTMScan HS phopshotyrosine kit (Cell Signaling Technology, #38572) following in-solution tryptic digestion and desalting ...
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bioRxiv - Biochemistry 2023Quote: ... 53BP1FFR proteins (WT and 53BP1-PN) and γH2A.X were stained respectively with HA-Tag (C29F4) rabbit antibody (Cell Signaling Technology) and anti-ψH2A.X (Ser139 ...
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bioRxiv - Cancer Biology 2024Quote: Whole cell protein lysates were isolated by resuspending cell pellets in 1x Cell lysis buffer (Cell Signaling Technologies, Danvers, MA) with added CompleteTM Protease Inhibitor Cocktail (Millipore Sigma ...
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bioRxiv - Cancer Biology 2024Quote: ... A horseradish peroxidase-conjugated β-actin antibody was used to detect this protein loading control (clone 8H10D10, #12262 Cell Signaling).
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bioRxiv - Biochemistry 2024Quote: ... and subsequently incubated with Alexa647-labelled anti-Protein C tag antibody and Alexa488-labelled anti-HA antibody (Cell Signaling Technology) for 15 min at 4 °C ...