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Citations for Cell Signaling Technology :
801 - 850 of 852 citations for Human Bcl2 Associated Death Promoter BAD ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: CUT&RUN assay was conducted following our prior protocol[51] using 200,000 MuSCs or IMR-90 cells with the CUT&RUN assay kit (Cell Signaling Technology, 86652). In brief ...
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bioRxiv - Cell Biology 2024Quote: Hif2α and ARNT chromatin immunoprecipitation (ChIP) assays were performed using a SimpleChIP® Plus Sonication Chromatin IP Kit (Cell Signaling Technology #56383) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... The CDK and cyclin antibodies were used from the CDK and Cyclin Antibody Sampler Kits (9868 and 9869, respectively; Cell Signaling Technology).
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bioRxiv - Molecular Biology 2024Quote: ... Immunocapture of GG-modified peptides was then performed using the PTMScan® Ubiquitin Remnant Motif (K-ε-GG) Kit (Cell Signaling Technology) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: Cleavage Under Targets and Release Using Nuclease (CUT&RUN) sequencing was performed using the assay kit from Cell Signaling Technology (cat# 86652). Briefly ...
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bioRxiv - Genomics 2024Quote: ... Separate PCLS were fixed for 30 minutes in the fixative solution from the β-galactosidase staining kit (Cell Signaling Technology Cat#9860) and then washed in PBS.
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bioRxiv - Cell Biology 2024Quote: The enrichment of di-Gly peptides was performed using PTMScan® HS Ubiquitin/SUMO Remnant Motif (K-GG) Kit (Cell Signaling Technology) as previously described99 ...
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bioRxiv - Developmental Biology 2024Quote: ... assays to detect DNA binding of NKX2.2 and KLF4 were conducted using the Cell Signaling Technologies Cut&Run Assay Kit (Cell Signaling Technology, 86652S). 100,000 live cells per Cut&Run preparation were fixed using 0.1% formaldehyde for 2 minutes ...
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bioRxiv - Developmental Biology 2024Quote: ... slides were washed with 1X PBST and incubated in a 1:300 dilution of AlexaFluor 594-conjugated rabbit anti-SIX1 (generated using Alexa Fluor 594 Antibody Labeling Kit (Thermo, A20185) and a 1 mg/mL concentrated anti-rabbit SIX1 (Cell Signaling, D5S2S)) ...
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bioRxiv - Cancer Biology 2021Quote: ... anti-PSA/KLK3 (D6B1) PARP (9542) and cell cycle regulation sampler kits (9932 and 9870) were obtained from Cell signaling technologies (Danvers, MA). The secondary antibodies (IR dye 680 and IR dye 800 ...
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bioRxiv - Cancer Biology 2021Quote: ... or of 35 mg (for mouse tumors) total proteins using the PTMScan® Phospho-Tyrosine Rabbit mAb (P-Tyr-1000) Kit (Cell Signaling Technology), according to manufacturer’s protocol ...
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bioRxiv - Genomics 2020Quote: Cells were treated with doxycycline for 7 days and were fix and stained with a Senescence β-Galactosidase Staining Kit (Cell Signaling, 9860) according the manufacture’s instruction and incubated at 37 °C for overnight.
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bioRxiv - Cancer Biology 2020Quote: ... Samples were alkylated and digested with trypsin at 37°C overnight and ubiquitinated peptides were enriched with PTMScan Ubiquitin Remnant Motif (K-ε-GG) Kit (Cell Signaling Technology). After Sep Pak desalting ...
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bioRxiv - Cancer Biology 2020Quote: ... 90% of the pre-cleared lysates were subsequently added to prewashed glutathione agarose beads from Active Ras Detection kit (Cell signaling Technology, #8821) for 1 hour at 4 °C under constant rocking ...
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bioRxiv - Neuroscience 2021Quote: SA-β-gal staining was performed according to the protocol of the Senescence-β-Galactosidase Staining Kit (Cell Signaling Technology, Danvers, MA, USA). At least 200 cells were counted in each treatment group from three independent experiments.
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bioRxiv - Molecular Biology 2020Quote: ... Cell pellet was then resuspended in 1 X PBS and cell fractionation was carried out using the Cell Fractionation Kit (Cell Signaling Technology, 9038) according to manufacturer’s protocol.
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bioRxiv - Cancer Biology 2020Quote: ... The proportion of senescent cells was determined by staining for β-galactosidase activity using Senescence β-Galactosidase Staining Kit (9860, Cell Signaling Technology) as described42.
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bioRxiv - Cancer Biology 2024Quote: ... The rest of the eluate was dried and enriched for acetylated peptides using the PTMScan® HS Acetyl-Lysine Motif (Ac-K) Kit (Cell Signaling Technology) as described in the manual ...
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bioRxiv - Cancer Biology 2022Quote: SUM159 and MDA-MB-231 cells were stained for SA-β-gal detection using a Senescence β-galactosidase Staining Kit (#9860, Cell Signaling). Staining was performed by following the manufacturer’s instructions and incubating the stained samples for 48hrs at 37°C without CO2 ...
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bioRxiv - Cell Biology 2023Quote: ... Cellular lysates from FLSs were harvested and a ChIP assay was performed using the SimpleChIP® Plus Enzymatic Chromatin IP Kit (9005, Cell Signaling Technology). In brief ...
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bioRxiv - Systems Biology 2024Quote: The enrichment of lysine acetylated peptides was performed using a PTMScan HS Acetyl-Lysine Motif [Ac-K] Kit (Cell Signaling Technology, Cat #50071) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were incubated with an appropriate HRP-conjugated secondary antibody (Cell signalling technologies, cat. n. 7074) and LumiGLO® ECL kit (Cell Signaling Technology) was employed following manufacturer’s instruction before to exposure to X-ray film (CytivaTM) ...
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bioRxiv - Cancer Biology 2023Quote: ... Anti-rat secondary antibody was used for detection of primary antibodies and visualized by SignalStain DAB Substrate kit (Cell Signaling Technology, Danvers, MA). All stained tissues were blindly evaluated by pathologists ...
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bioRxiv - Cancer Biology 2023Quote: ... Frozen sections were hydrated with PBS for 10min at RT and stained using Senescence β-Galactosidase staining kit according to manufacturer’s instructions (Cell signaling technology, 9860). The sections were incubated at 37℃ in a dry incubator (no CO2 ...
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Ciliopathy interacts with neonatal anesthesia to cause non-apoptotic caspase-mediated motor deficitsbioRxiv - Neuroscience 2024Quote: ... Cryosections were used for slide-mounted immunohistochemistry for NeuN and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL Assay Kit, Cell Signaling Technologies #64936). The free floating immunohistochemical procedure consisted of incubating the vibratome slices in donkey serum blocking solution (donkey serum in 0.2% Triton-X in 0.01M PBS ...
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bioRxiv - Biochemistry 2024Quote: ... Peptides were later enriched using di-Gly antibody immunoprecipitation using the PTMScan Ubiquitin Remnant Motif (K-ε-GG) Kit (Cell Signaling catalog# 5562). Post immunoprecipitation ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples were dried by lyophilization and resuspended in IAP buffer from PTMScan HS Ubiquitin/SUMO Remnant Motif (K-ε-GG) Kit (Cell Signaling #59322). K-GG containing heavy reference peptides were pooled and diluted into the IAP buffer resuspension fat a final concentration of 1 fmol per peptide ...
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bioRxiv - Molecular Biology 2024Quote: ... tumors were fixed and SA-β-gal whole mount staining was performed overnight according to the procedures described in the Senescence Beta-Galactosidase Cell Staining Kit (Cell Signaling Technology #9860). All incubations and washes were performed with fresh and filtered solutions ...
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bioRxiv - Cancer Biology 2024Quote: ... p-c-Myc (1:500, #94015), p-β-catenin (1:300, #9564) and Ferroptosis Antibody Sampler Kit (#29650) were obtained from Cell Signaling Technology. α-4HNE was from Abcam (1:200 ...
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bioRxiv - Genetics 2021Quote: ... and myotube (4 days differentiation in low serum differentiation medium) and ChIP was performed using the Simple ChIP Enzymatic Chromatin IP Kit (Cat# 9003; Cell Signaling Technology; Danvers, MA) following the manufacturer’s protocols ...
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bioRxiv - Developmental Biology 2020Quote: ... stained with anti-F4/80-PE antibody (eBiosciences #12-4801-80) and isolated using an EasySep™ mouse PE positive selection kit that uses a magnetic bead-based purification strategy (Cell Signaling Technologies #17656) and in the presence of 2mM Icam4/av inhibitor peptide (Xue et al. ...
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bioRxiv - Neuroscience 2021Quote: Approximately 1.2 million microglia were exposed to the indicated treatments followed by cell preparation according to the SimpleChIP® Enzymatic Chromatin IP Kit (Cell Signaling Technology, #9003). Samples were cross-linked for 10 min at room temperature with 1% formaldehyde solution ...
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bioRxiv - Cell Biology 2020Quote: ... Specificity of C12FDG detecting senescent cells was determined in comparison with results obtained with the senescence β-galactosidase staining kit (Cell Signaling Technology #9860) on IMR90 cells that were treated with 100 ng/mL doxorubicin (Sigma ...
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bioRxiv - Molecular Biology 2021Quote: Enrichment of K-ε-GG peptides was performed with the PTMScan ubiquitin remnant motif (K-ε-GG) kit (Cell Signaling Technology, cat. no. 5562) containing anti–K-ε-GG antibody cross-linked to protein A beads ...
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bioRxiv - Cancer Biology 2021Quote: ChIP was performed on 5X106 cells from 2-day control and radiated gliomaspheres using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) (Cell Signaling Technology, #9005S) according to manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: SA-β-gal staining was performed using a SA-β-gal staining kit according to the manufacturer’s protocol (Cell Signaling Technology, Danvers, MA, USA). In short ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein was extracted with RIPA buffer and processed for acetylomic mass spectrometry as described previously55 using PTMScan® HS Acetyl-Lysine Motif (Ac-K) Kit (Cell Signaling Technology #46784).
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bioRxiv - Molecular Biology 2022Quote: ... Peptides were enriched for malonylation with anti-malonyl antibody conjugated to agarose beads from the Malonyl-Lysine Motif Kit (Cell Signaling Technology, Danvers, MA). This process was performed according to the manufacturer protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... The following steps were performed according to the manual of the PTMScan® HS Ubiquitin/SUMO Remnant Motif (K-ε-GG) Kit (Cell Signaling #59322). In brief ...
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bioRxiv - Cancer Biology 2023Quote: IMR90 cells seeded on the plate were fixed and stained for SA-β-gal activity with the senescence β-Galactosidase Staining Kit according to the protocol (Cell Signaling, #9860S).
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bioRxiv - Molecular Biology 2023Quote: ... Apoptotic cells were detected by TUNEL (Terminal deoxynucleotidyl transferase dUTP nick end labeling) staining using in situ apoptosis detection kit (Takata Bio Inc., Shiga, Japan) or immunostaining of cleaved caspase-3 (Cell Signaling Technology, Danvers, MA).
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bioRxiv - Microbiology 2023Quote: ... peptide samples resuspended in IAP buffer were incubated with 100uL of antibody-bead conjugates specific for acetyl-lysine (Cell Signaling Technologies, PTMScan kits #13416). Immunoprecipitation proceeded overnight at 4°C with gentle mixing ...
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bioRxiv - Immunology 2022Quote: ... Cells were fixed and permeabilized with the Foxp3 transcription factor staining kit (eBioscience, 00-5523-00) prior intracellular staining with the indicated antibodies: H3K9ac (Cell Signaling, 9649, 1:3000), H3K27ac (Cell Signaling ...
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bioRxiv - Cancer Biology 2024Quote: RAS activity was assessed by affinity pull-down of endogenous levels of GTP-bound Active-Ras as described by Active Ras detection kit (Cell Signaling Technology, cat# 8821).
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bioRxiv - Cell Biology 2024Quote: ... that were treated with PBS or Dox or transduced with adenovirus harboring shRNA scramble (shCtrl) or shRNA Atg7 (shAtg7) were stained with the SA-β-gal staining kit (Cell Signaling Technologies, #9860), according to the protocol provided by the manufacturer ...
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bioRxiv - Molecular Biology 2024Quote: ... The rest was split into 8 aliquots dried and enriched for acetylated peptides using the PTMScan® HS Acetyl-Lysine Motif (Ac-K) Kit (Cell Signaling Technology #46784) as described in the manual and peptides were eluted twice with 200µL of 50% acetonitrile (ACN) ...
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bioRxiv - Neuroscience 2020Quote: ... Astrocytes were then collected by scraping and the CNMCS Compartmental Protein Extraction Kit (BioChain Cat# K3013010 Hayward, CA) with protease/phosphatase inhibitors (PI, Cell Signaling, Cat# 5872S, Danvers, MA) was used and the nuclear fractions were isolated for each treatment condition ...
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bioRxiv - Immunology 2024Quote: ... and kept at 4 °C for up to 10 days until chromatin fragmentation using SimpleChIP® Plus Sonication Chromatin IP Kit (Cell Signaling Technology, Danvers, MA). Briefly ...
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bioRxiv - Cell Biology 2023Quote: ... The resulting DNA fragment samples were generated into libraries using the SimpleChIP® ChIP-seq DNA Library Prep Kit for Illumina® (Cell Signaling Technology, 56795) and SimpleChIP® ChIP-seq Multiplex Oligos for Illumina® (Dual Index Primers ...
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bioRxiv - Cell Biology 2024Quote: ... mouse anti-GM130 (BD transduction Laboratories, ref:610822, 1:1000, rabbit ITGB1, ITGB4, ITGA4 (from integrin Ab Sampler kit, Cell signaling ref: 4749, 1:1000), rabbit anti-EGFR (Cell Signaling ref ...