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Citations for Cell Signaling Technology :
751 - 800 of 852 citations for Human Bcl2 Associated Death Promoter BAD ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... ChIP was performed using an amount of BEL-ChIP nuclear extract corresponding to 3.4 μg of input genomic DNA in 250 μL of ChIP Buffer + 1X PIC (SimpleChIP Kit #9003, Cell Signaling Technology). Antibody was added according to the vendor’s specifications and incubated overnight with end-over-end mixing at 4°C ...
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bioRxiv - Cancer Biology 2020Quote: ... All ChIP was performed using the SimpleChIP Enzymatic Chromatin IP kit with magnetic beads according to the manufacturer’s instructions (Cell Signaling Technologies). DNA levels were quantified by qPCR amplification using Power SYBR Green Mastermix (Applied Biosystems ...
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bioRxiv - Cancer Biology 2021Quote: GTP-bound GTPase pull-down assays were performed using the Active Rac1/CDC42 Detection Kit (Cell Signaling Technology, Cat. #8815, 8819). After transfection or verteporfin treatment ...
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bioRxiv - Neuroscience 2020Quote: ... or WT N2aC plated in chamber slides using the Annexin V-FITC Early Apoptosis Detection Kit (Cell Signaling Technology, Danvers, MA). As a positive control for apoptosis and necrosis ...
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bioRxiv - Developmental Biology 2023Quote: ChIP-qPCR and ChIP-seq samples were prepared following the instructions of the SampleChIPR Enzymatic Chromatin IP Kit (Cell Signaling, #9005). Briefly ...
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bioRxiv - Genomics 2023Quote: CUT&RUN experiments were conducted using the CUT&RUN (Cleavage Under Targets & Release Using Nuclease) assay kit (Cell Signaling Technology, #86652) following the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2023Quote: A glutathione assay was developed for in-chip measurements by adapting the Cellular Glutathione Detection Assay Kit (Cell Signaling Technology #13859). The monochlorobimane reagent was prepared by reconstitution in DMSO per manufacturer’s directions ...
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bioRxiv - Molecular Biology 2023Quote: The chromatin immunoprecipitation (ChIP) assay was performed with SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) (Cell signaling technology, #9005) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... Chromatin was sheared using a Covaris Sonicator for 20 minutes in sonication buffer (SimpleChIP Plus Sonication Kit, Cell Signaling Technology, #57976) to obtain fragments in the range of 200-500 bp ...
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bioRxiv - Developmental Biology 2022Quote: ... The soft palatal tissue from E13.5 embryos was collected and fixed according to the manufacturer’s instructions based on Cut and Run assay kit (Cell Signaling, 86652). Ten μl of IgG isotype control (Cell Signaling ...
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bioRxiv - Cancer Biology 2022Quote: β-Galactosidase staining at pH 6.0 was performed with 5 × 105 cells using the Senescence β-Galactosidase Staining kit (Cell Signaling # C10841) according to the manufacturer’s protocol.
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bioRxiv - Neuroscience 2023Quote: 25 mg cortical tissues were dissected from wild type mice (n = 3) and chromatin was prepared using the SimpleChIP plus Enzymatic Chromatin IP Kit (Cell Signaling). UBE3A-bound chromatin was immunoprecipitated using rabbit anti-UBE3A antibody (Bethyl Laboratories) ...
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bioRxiv - Genetics 2022Quote: CUT&RUN (Cleavage Under Targets & Release Using Nuclease) libraries were prepared with the established CUT&RUN Assay Kit (Cell Signaling, #86652S). The following antibodies were used ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell viability was measured after 24 hours of AraC treatment using the XTT Cell Viability Kit from Cell Signaling Technology (#9095) and a Tecan Infinite 200 Pro plate reader ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were stained for β-galactosidase (β-Gal) as a marker of senescence using a commercial kit (#9860S; Cell Signaling Technology) and imaged under an inverted brightfield microscope at 10X magnification ...
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bioRxiv - Immunology 2024Quote: ... The sections were incubated with anti-PLA2G12A antibody (clone #44) biotinylated with a Biotin-Labeling Kit (Dojindo) and rabbit anti-CD4 monoclonal antibody (D7D2Z; Cell Signaling) diluted at 1:1000 at room temperature for 1 h ...
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bioRxiv - Molecular Biology 2024Quote: CUT&RUN (Cleavage Under Targets & Release Using Nuclease) was performed according to the CUT&RUN Assay Kit protocol (Cell Signaling Technology) with the following modifications ...
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bioRxiv - Cancer Biology 2024Quote: Detection of SA-β-gal activity on adherent cells was done using the Senescence β-Galactosidase Staining Kit (#9860, Cell signaling), according to manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were fixed and stained for β-Galactosidase following the protocol of the Senescence β-Galactosidase Staining Kit (Cell Signaling Technologies). Following staining ...
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bioRxiv - Cancer Biology 2024Quote: Chromatin immunoprecipitation (ChIP) was performed using the SimpleChIP® Enzymatic Chromatin Magnetic Bead IP Kit per manufacturer’s protocol (Cell Signaling #9003). At least 4×106cells were plated in quadruplicate in 10 cm dishes and grown for ∼2 days until they began to form small spheres ...
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bioRxiv - Cell Biology 2020Quote: K-ε-GG remnant containing peptides were enriched using the PTMScan® Ubiquitin Remnant Motif (K-Є-GG) Kit (Cell Signaling Technology). Crosslinking of antibodies to beads and subsequent immunopurification was performed with slight modifications as previously described [62] ...
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bioRxiv - Developmental Biology 2021Quote: ChIP assays were performed in WT and Arp5-KO RD cells using the SimpleChIP Plus Enzymatic Chromatin IP Kit (Cell Signaling Technology) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... quantified with Quantitative Colorimetric Peptide Assay (Thermo Fischer Scientific) and dried on Speed-Vacuum before enrichment with Ubiquitin Remnant Motif (K-ε-GG) Kit (Cell Signaling). After Sep Pak desalting ...
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bioRxiv - Neuroscience 2021Quote: Antibodies on these sections included Rat anti-CD3 epsilon (1:100, NBP1-26685, Novus Bio) and Rabbit anti-C-Kit (1:100, D13A2, Cell Signaling Tech).
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bioRxiv - Physiology 2020Quote: ChIP assays of frozen liver tissues of humanized mice were performed using a Simple ChIP Enzymatic Chromatin IP kit (Cell Signaling Technology) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2020Quote: ... 5 days after irradiation the plates were assayed for senescence via the Senescence β-galactosidase Cell Staining Kit (Cell Signaling, Catalog # 9860) and the staining was performed as per manufacturer’s protocol.
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bioRxiv - Plant Biology 2020Quote: ... Immuno-precipitation was performed for two replicates for each genotype (about 1 g tissue/IP) by SimpleChIP Plus Kit (Cell Signaling Technology) according to the manufacturer’s instructions ...
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Hydrogen sulfide blocks HIV rebound by maintaining mitochondrial bioenergetics and redox homeostasisbioRxiv - Microbiology 2021Quote: The chromatin immunoprecipitation (ChIP) assays was performed using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) (Cell Signaling Technologies, Inc., MA, US) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ChIP-seq experiments were performed with 108 HDFs per replicate using a SimpleChip Plus sonication chromatin IP kit (Cell Signaling Technology, 56383) according to the manufacturer directions ...
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bioRxiv - Neuroscience 2022Quote: ... mouse anti TRA-1-81 (1:200) and mouse anti-SSEA4 (1:200) (all from Cell Signaling Technology, StemLight Pluripotency Antibody Kit) to assess for pluripotency ...
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bioRxiv - Molecular Biology 2022Quote: Lysates from parental and resistant cells were used to screen activation of receptor tyrosine kinases using the PathScan® RTK Signaling Antibody Array Kit (7982, Cell Signaling) following the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2022Quote: ... Peptides were enriched for acetylation with anti-acetyl antibody conjugated to agarose beads (Acetyl-Lysine Motif Kit; Cell Signaling Technology, Danvers, MA). This process was performed according to the manufacturer protocol ...
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bioRxiv - Cancer Biology 2022Quote: Fragmental DNA for ChIP-seq was prepared using SimpleChIP®Plus Enzymatic Chromatin IP Kit (Magnetic Beads) (Cell Signaling Technologies, Cat#9005) per manufacturer’s instructions ...
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bioRxiv - Pathology 2021Quote: ... was used as secondary for 30 minutes and the signal was developed using the SignalStain DAB Substrate Kit (Cell Signaling, Danvers, MA).
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bioRxiv - Immunology 2020Quote: ... Cells were subsequently washed three times with ice-cold PBS and subcellular fractionation was carried out using a Cell Fractionation kit following manufacturer instructions (Cell Signaling, #9038).
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bioRxiv - Cell Biology 2021Quote: Acylated (either acetylated or malonylated) peptides were enriched by immunoaffinity purification using the PTMScan Acetyl-Lysine or PTMScan Malonyl-Lysine Kits (Cell Signaling Technology) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: Proliferating and Senescent mouse fibroblasts were washed twice with 1X PBS and fixed and stained for B-Gal activity using the Senescence B-Galactosidase Staining Kit per the manufacturer’s instructions (Cell Signaling Technology 9860S). The following day cells were washed twice with 1X PBS and stained with Hoechst (Thermo Fisher Scientific 62249) ...
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bioRxiv - Cell Biology 2021Quote: The staining of SA-β galactosidase in cells at pH-6 was performed using a senescence β-Galactosidase staining kit according to the instructions of the manufacturer (Cell signaling). Measurement of the absorbance at 420 nm from SA-β galactosidase activity detectable at pH 6.0 was performed as described (Lee et al. ...
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bioRxiv - Immunology 2022Quote: ... Fly heads were cut off and placed into lysis buffer from PTMScanHS Ubiquitin/SUMO Remnant Motif Kit Protocol (#59322, Cell Signaling, UK), that contained 5% SDS in TEAB buffer (T7408 ...
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bioRxiv - Cancer Biology 2023Quote: ... for 1 hr at room temperature followed by development using chromogenic substrate DAB (SignalStain® DAB Substrate Kit, Cell Signaling, Cat# 8059s) for 10 min at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... Enrichment for ubiquitinated peptides was conducted using a PTM Scan® Ubiquitin Remnant Motif [K-ε-GG] kit (Cell Signaling, Danvers, MA) following the vendor’s recommended procedures ...
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bioRxiv - Physiology 2022Quote: The chromatin immunoprecipitation assay was performed as previously reported using the SimpleChIP Enzymatic Chromatin IP Kit (Cell Signaling Technology, Cat No. 9003)47 ...
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bioRxiv - Molecular Biology 2024Quote: ... freshly prepared by trypsin-EDTA digestion were used to perform CUT&RUN with the CUT&RUN Assay Kit (catalog no. 86652, Cell Signaling Technology), according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: 200,000 primary NSCs either stimulated with IL1α (10ng/ml) or left unstimulated were used for CUT&RUN assay using the CUT&RUN kit from Cell Signaling Technologies® #86652 according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were rinsed with pre-warmed 1x PBS and fixed with 1x fixative solution provided by senescence beta-galactosidase staining kit (9860, Cell Signaling Technology) for 15 minutes ...
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bioRxiv - Cancer Biology 2022Quote: ... and acetylation were sequentially enriched and purified using PTMScan® Phospho-Tyrosine Rabbit mAb (P-Tyr-1000) Kit (Cell Signaling Technology #8803), PTMScan® Ubiquitin Remnant Motif (K-ε-GG ...
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bioRxiv - Bioengineering 2023Quote: A reduced glutathione assay was developed for in-chip measurements by adapting the Cellular Glutathione Detection Assay Kit (Cell Signaling Technology #13859). The monochlorobimane reagent was prepared by reconstitution in DMSO per manufacturer directions ...
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bioRxiv - Cancer Biology 2022Quote: The 1 mg TMT-labeled peptide fraction underwent phosphotyrosine peptide enrichment using the PTMscan HS Phospho-Tyrosine (P-Tyr-1000) Kit (Cell Signaling Technology) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... blocked and probed with the indicated antibodies (Rb Antibody Sampler Kit #9969) and β-actin from Cell Signaling Technologies (Danvers, Massachusetts, USA).
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bioRxiv - Cancer Biology 2023Quote: ... cellular and nuclear fractions from HNSCC cell lines and tissues were prepared using the cell fractionation kit purchased from Cell Signaling (#9038) following the manufacturer’s instructions ...