6 4 METHOXY PHENYL 3 METHYL IMIDAZO 2 1 B THIAZOLE 5 CARBALDEHYDE citations for Cell Signaling Technology

DNA density is a better indicator of a nuclear bleb than lamin B loss

Samantha Bunner, et al., bioRxiv - Cell Biology 2024

Quote: ... The secondary antibody solution contained goat anti-mouse antibodies FITCI at 1:1000 dilution and goat anti-rabbit dilution TRITCI at 1:1000 dilution (from Cell Signaling Technologies) and incubated in humidity chambers at 37°C for 30 minutes ...

A novel PSMB8 isoform associated with multiple sclerosis lesions induces P-body formation

Benjamin C. Shaw, Jessica L. Williams, bioRxiv - Molecular Biology 2024

Quote: ... for 1 h at room temperature and blotted with an antibody against phosphorylated upstream frameshift protein 1 (phospho-UPF1) (pUPF1; 1:1000, Cell Signaling Technologies) overnight at 4°C ...

Haploidy-linked cell proliferation defects limit larval growth in Zebrafish

Kan Yaguchi, et al., bioRxiv - Developmental Biology 2024

Quote: ... Alexa Fluor 488-conjugated rabbit monoclonal anti-phospho-histone H3 (pH3) (1:200 for IF and 1:50 for flow cytometry; D2C8; Cell Signaling Technology); and fluorescence (Alexa Fluor 488 or Alexa Fluor 594 ...

Mechanotransductive feedback control of endothelial cell motility and vascular morphogenesis

Devon E. Mason, et al., bioRxiv - Cell Biology 2024

Quote: ... Primary antibodies were detected secondaries antibodies diluted in PBS with 1% goat serum: polyclonal Alexafluor 594-conjugated anti-rabbit IgG (1:1000, Cell Signaling, 8889), and polyclonal Alexafluor 488-conjugated anti-mouse IgG (1:1000 ...

Loss of Lkb1 cooperates with Braf^V600E and UV radiation increasing melanoma multiplicity and neural-like dedifferentiation

Kimberley McGrail, et al., bioRxiv - Cancer Biology 2024

Quote: ... p-S6S235/236 (#2211; 1:500) and p-4EBP1T37/46 (#2855; 1:1000) antibodies were obtained from Cell Signaling (Danvers, MA, USA). Cre antibody (NB100-56135 ...

Leveraging the p53 signaling pathway as a radio-sensitization strategy in endometrial cancer

Roberto Vargas, et al., bioRxiv - Cancer Biology 2023

Quote: ... 1:3000), anti-p21 Waf1/Cip1 (clone 12D1, #2947, 1:3000), anti-Vinculin (clone E1E9V, #13901, 1:5000) were from Cell Signaling Technology. Goat anti-rabbit antibody linked to horseradish peroxidase (HRP ...

Topological design principle for the robustness of necroptosis biphasic, emergent, and coexistent (BEC) dynamics

Fei Xu, et al., bioRxiv - Systems Biology 2023

Quote: ... Anti-caspase-8 antibody (4790, dilution 1:1000) and anti-cleaved caspase-8 antibody (8592, dilution 1:500) were purchased from Cell Signaling Technology. Anti-p-RIP3 antibody (ab222320 ...

Neuron-astrocyte metabolic coupling facilitates spinal plasticity and maintenance of persistent pain

Sebastián Marty-Lombardi, et al., bioRxiv - Neuroscience 2022

Quote: ... The sections were incubated with primary antibody (pS6 Cell Signaling 2215, 1:1000; GFAP Cell Signaling #3670, 1:500; NeuN Cell Signaling D4G40 ...

In vivo functional genomics identifies essentiality of potassium homeostasis in medulloblastoma

Jerry J. Fan, et al., bioRxiv - Cancer Biology 2023

Quote: ... Membranes were then washed in TTBS/1% skim milk, incubated with secondary antibodies (1:5000 anti-mouse IgG, HRP-linked antibody (Cell Signaling #7076S)) ...

Molecular cartography uncovers evolutionary and microenvironmental dynamics in sporadic colorectal tumors

Cody N. Heiser, et al., bioRxiv - Systems Biology 2023

Quote: ... rinsed in wash buffer and incubated for one hour in the primary antibodies (PAK4 Santa Cruz Biotechnology sc-390507 1:75 primary dilution, TGFBI Abcam ab170874 1:300 primary dilution, DDR1 Cell Signaling #5583 primary dilution 1:2000 ...

polo affects cell fate upon ionizing radiation in Drosophila hematopoietic progenitors by negatively regulating lok

Tram Thi Ngoc Nguyen, et al., bioRxiv - Developmental Biology 2023

Quote: ... The following primary antibodies were used in this study: rabbit anti-cleaved Dcp-1 (Asp215) (1:250, Cell Signaling, Danvers, MA, USA), rabbit anti-peroxidasin (Pxn ...

Nutrient deprivation differentially affects gene expression, immunity, and pathogen susceptibility across symbiotic states in a model cnidarian

Maria Valadez-Ingersoll, et al., bioRxiv - Genomics 2023

Quote: ... The membrane was washed five times with TBST and then incubated for 1 h with secondary goat-anti-rabbit-HRP conjugate (1:4000, Cell Signaling Technology) in TBST with 5% non-fat powdered milk ...

Transcriptional suppression of sphingolipid catabolism controls pathogen resistance in C. elegans

Mohamad A. Nasrallah, et al., bioRxiv - Immunology 2023

Quote: ... blocked with 5% fat-free milk in TBST and probed with a 1:1000 dilution of an antibody that recognizes the doubly-phosphorylated TGY motif of PMK-1 (Cell Signaling Technology) or a monoclonal anti-tubulin antibody (Sigma-Aldrich ...

Radiation therapy promotes unsaturated fatty acids to maintain survival of glioblastoma

Mara De Martino, et al., bioRxiv - Cell Biology 2022

Quote: ... m-h-anti-CHOP 1:1000, m-h-anti-fatty acid synthase 1:100, cat#3180T; m-h-anti-vinculin 1:1000, cat#13901S; Cell Signaling Technology) at 4°C overnight ...

H3.1^Cys96 oxidation by mitochondrial ROS promotes chromatin remodeling, breast cancer progression to metastasis and multi-drug resistance

Flavio R. Palma, et al., bioRxiv - Cancer Biology 2022

Quote: ... Membranes were incubated with appropriate primary antibodies (phospho-ATM, 1:1000, Cell Signaling Technology, #13050; E-Cadherin, 1:1000, Cell Signaling Technology, #3195 ...

SARS-CoV-2 spike-induced syncytia are senescent and contribute to exacerbated heart failure

Luming Wan, et al., bioRxiv - Cell Biology 2023

Quote: ... anti-phospho-IRF3 (4947S, 1:1,000 dilution) and anti-phospho-TBK1 (5483T, 1:2,000 dilution) were purchased from Cell Signaling Technology (Massachusetts, USA). Anti-phospho-p65 (A19653 ...

Quantitative flow cytometric selection of tau conformational nanobodies specific for pathological aggregates

Jennifer M. Zupancic, et al., bioRxiv - Bioengineering 2023

Quote: ... Yeast cells were combined with QD-fibril complexes in a total volume of 200 μL with 1% milk and 1:1000 mouse anti-Myc antibody (Cell Signaling, 2276S) and allowed to incubate with end-over-end mixing at room temperature for approximately 3 h ...

SPLIT HAND/FOOT VARIANTS FAIL TO RESCUE PRDM1A MUTANT CRANIOFACIAL DEFECTS

Brittany T. Truong, et al., bioRxiv - Developmental Biology 2023

Quote: ... and incubated overnight at 4°C with rotation in the respective antibody (IgG [1:100; Jackson ImmunoResearch, 111-005-003, RRID: AB_2337913], H3K27ac [1:66; Cell Signaling Technology, 4353S ...

Inhibiting endothelial cell Mst1 attenuates acute lung injury in mice

Zhi-Fu Guo, et al., bioRxiv - Physiology 2023

Quote: ... 1:1000 dilution), antianti-p-MOB1 (#8699, 1:1000 dilution), and anti-MOB1 (#13730, 1:1000 dilution) antibodies were purchased from Cell Signaling Technology. Anti-phospho-Mst1 (AP0906 ...

A TrkB and TrkC partial agonist restores deficits in synaptic function and promotes activity-dependent synaptic and microglial transcriptomic changes in a late-stage Alzheimer’s mouse model

Amira Latif-Hernandez, et al., bioRxiv - Neuroscience 2023

Quote: ... rabbit polyclonal CaMKII (1:2000, 3362), rabbit monoclonal anti-phopho-GluA1Ser831 (1:2000, 75574) and rabbit monoclonal GluA1 (1:2000, 13185) purchased from Cell Signaling Technology, Inc. ...

Injury induced renal fibrosis promotes cystogenesis and cyst growth in adult mice with autosomal dominant polycystic kidney disease

Ming Wu, et al., bioRxiv - Pathology 2023

Quote: ... 0.01%Tween 20) for 1 hour at room temperature and was followed by incubation with anti-EZH2 (1:1000, 5246S, Cell Signaling Technology) antibody ...

GCN5L1-mediated acetylation prevents Rictor degradation in cardiac cells after hypoxic stress

Paramesha Bugga, et al., bioRxiv - Cell Biology 2023

Quote: ... Cat. 97166 [D4C6R], mouse mAb, 1:1000; Akt, Cell Signaling, Cat. 4685 [11E7], rabbit mAb, 1:1000; p-Akt [Ser473], Cell Signaling, Cat ...

C-type natriuretic peptide improves maternally aged oocytes quality by inhibiting excessive PINK1/Parkin-mediated mitophagy

Hui Zhang, et al., bioRxiv - Developmental Biology 2023

Quote: ... followed by incubation with primary antibodies at 4 °C overnight (the primary antibodies were rabbit anti-GAPDH antibody, 1:2000, Cell Signaling, Cat#5174; rabbit anti-p62 antibody, 1:1000, Cell Signaling, Cat#23214 ...

The renal capsule, a vibrant and adaptive cell environment of the kidney in homeostasis and aging

Ben Korin, et al., bioRxiv - Immunology 2023

Quote: ... The Ce3D™ Permeabilization/Blocking Buffer was discarded and replaced with Alexa Fluor 647 conjugated anti-mouse Iba-1 (1:100, 78060S, Cell Signaling Technology) in Ce3D™ Antibody Diluent Buffer (48 hr ...

Synaptic Activity Causes Minute-scale Changes in BAF Complex Composition and Function

S. Gourisankar, et al., bioRxiv - Molecular Biology 2023

Quote: ... then stained with 1:2000 chicken anti-GFP (Aves #GFP-1020) for visualization of dendrites and 1:3200 rabbit anti-cFos (Cell Signaling 9FG) to validate stimulation ...

Unravelling biological processess and EGFR pathway regulation by the receptor-like protein tyrosine phosphatase PTPRH in non-small cell lung cancer

Mylena M. O. Ortiz, et al., bioRxiv - Cancer Biology 2024

Quote: ... The coverslips were then incubated with primary antibodies against BioID2 (1:5000, BioFront #BID2-CP-100) or HA tag (1:800, Cell Signaling #3724) diluted in 1% BSA/ PBS-Tween 0.1% for one hour in a humidified chamber at RT ...

Engineered 3D Hydrogel Matrices to Modulate Trophoblast Stem Cell-Derived Placental Organoid Phenotype

Emily M. Slaby, et al., bioRxiv - Bioengineering 2024

Quote: ... Hydrogels were stained with primary antibodies (mouse anti-KRT7, 1:300, NSJ Bioreagents catalog #V2656; rabbit anti-Ki67, 1:400, Cell Signaling catalog #9129 ...

Inhibition of Inflammatory Osteoclasts Enhances CGRP⁺TrkA⁺ Signaling and Accelerates Callus Remodeling in Osteoporotic Fractures

Yuexia Shu, et al., bioRxiv - Pathology 2024

Quote: ... the cells are stained with specific fluorescent dyes using antibodies targeting Cx3cr1 (1:100, NBP2-68616, Novus) and Ly6C (1:100, 88920, Cell Signaling Technology). Once stained ...

LEDGF interacts with the NID of MeCP2 and modulates MeCP2 condensates

Saskia Lesire, et al., bioRxiv - Molecular Biology 2024

Quote: ... Subsequently membranes were incubated with primary antibodies (1:500 rabbit anti-LEDGF-PWWP [Abcam; ab177159], 1:1000 rabbit anti-MeCP2 [Cell Signaling Technology; 3456S] ...

T cell responsiveness to IL-10 defines the immunomodulatory effect of costimulation blockade via anti-CD154 and impacts transplant survival

Marcos Iglesias, et al., bioRxiv - Immunology 2024

Quote: ... Protein expression levels were detected with the following antibodies: Jak-1 (6G4) and phospho-Jak-1 (Tyr1034/1035) (from Cell Signaling Technology), IL-10Rα (A3 ...

A series of dual-reporter vectors for ratiometric analysis of protein abundance in plants

Aashima Khosla, et al., bioRxiv - Plant Biology 2020

Quote: ... and horse anti-mouse (1:5000, Cell Signaling, #7076). Blots were developed using an Azure Radiance Plus chemiluminescent substrate (#AC2102).

SARS-CoV-2 infection induces a pro-inflammatory cytokine response through cGAS-STING and NF-κB

Christopher J. Neufeldt, et al., bioRxiv - Microbiology 2020

Quote: ... Rabbit anti-IkB (Cell Signaling: 9242s, WB - 1:1000); Sheep and-TGN46 (Biorad:AHP500G ...

Nucleus translocation of tRNA synthetase mediates late integrated stress response