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Citations for Cell Signaling Technology :
7301 - 7350 of 8705 citations for Alpaca ZAP Antibody Internalization Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... the membrane was washed 3 times with TBS-T and incubated with horseradish peroxidase-conjugated secondary antibody (Cell Signaling Technology #7074) for 1 hour ...
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bioRxiv - Cancer Biology 2022Quote: ... The chromatin was then incubated with 5 µg of antibody against KDM6A (A302-374A, Bethyl Laboratories and 33510S, Cell Signaling Technology), KDM6B (ab169197 ...
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bioRxiv - Cancer Biology 2022Quote: ... The separated proteins on the gel were transferred onto PVDF membrane and were probed for specific antibodies against Rb (cat# 9309; Cell signaling) phospho-Rb (cat# 8516 ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were incubated with the primary antibodies rabbit anti-phospho-β catenin (Ser675, 1:100, D2F1, Cell Signaling Technology, MA, USA) and mouse anti-β-catenin (1:100 ...
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bioRxiv - Biochemistry 2022Quote: ... The membranes were then probed with goat anti-rabbit and goat anti-mouse secondary antibodies (#7074, #7076, Cell Signaling, Danvers, MA), and blots were incubated with Clarity Western ECL Substrate (Cat ...
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bioRxiv - Molecular Biology 2022Quote: ... The membrane was then transferred to a secondary antibody solution containing a 1:5000 dilution of α-mouse HRP (Cell Signaling) for one hour of shaking at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... Then the sections were incubated with primary antibody of interest in the blockage solution (c-fos,1:200, Abcam/ab208942; NFκB-p65, 1:200, Cell Signaling Technology/8242 ...
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bioRxiv - Biochemistry 2022Quote: ... We incubated coverslips with primary antibodies diluted in goat serum (Anti-HA, Roche, ref #11867423001; Anti-Tom20, Cell Signaling, ref #42406S) in a humid chamber overnight at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... We also used the following commercial antibodies for western blotting and/or immunofluorescence staining: rabbit anti-WASF3 (#2806; Cell Signaling Technology), mouse anti-ARP3 (ab49671 ...
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bioRxiv - Cancer Biology 2022Quote: ... Chemiluminescent detection was performed with HRP-conjugated secondary antibodies (Santa Cruz Biotechnology Inc., catalog sc-2004; Cell Signaling Technology, catalog 7076S) and developed using Genemate Blue Ultra-Autoradiography film (VWR ...
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bioRxiv - Cell Biology 2022Quote: ... and 1:5000 dilution of HRP-conjugated rabbit anti-mouse secondary antibody (Cat. No. 7076, Lot No. 36, Cell Signaling Technology), and the blot was developed similarly.
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bioRxiv - Cell Biology 2022Quote: ... followed by blocking in 5% BSA with 0.2% Triton X-100 for 30min and incubation with primary antibody Axin1 (#2087, 1:200, Cell Signaling Technology) overnight ...
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bioRxiv - Cancer Biology 2022Quote: ... 2021) using 25μg of chromatin and 10ug of either anti-HIF1α ChIP antibody (Novus) or anti-rabbit IgG (Cell Signaling Technologies).Chromatin was immunoprecipitated overnight at 4C and HIF1α binding sites assayed via RT-qPCR using 2μl of either input ...
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bioRxiv - Developmental Biology 2022Quote: ... Primary antibodies used in this study were as follows: rabbit monoclonal active β-catenin (Cell Signaling, 19807S, 1:100 with TSA), mouse monoclonal ...
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bioRxiv - Microbiology 2022Quote: ... The cells were harvested at 48 h pt and equal amounts of proteins were analyzed by western blot for p-eIF2α or total eIF2α (both antibodies were obtained from Cell Signaling). Expression of E was detected using an HA-tag antibody (Invitrogen) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and rabbit antibodies specific for p-Erk1/2 (phospho-p44/42 MAPK) (1:1000, #4370; Cell Signaling Technology, Beverly, MA, USA), Erk1/2 (p44/42 MAPK ...
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bioRxiv - Pathology 2022Quote: ... blots were transferred to a solution containing anti-rabbit IgG HRP-linked secondary antibody (1:1000 dilution; Cell Signaling Technology 7074S) and incubated for 1 hour at room temperature ...
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bioRxiv - Plant Biology 2022Quote: ... the membrane was incubated overnight at 4°C with rabbit anti-phospho-acetyl CoA carboxylase (Ser79) antibody (#3661; Cell Signaling Technology Europe BV ...
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bioRxiv - Neuroscience 2022Quote: ... equal volumes of each fraction were analyzed by Western blotting with the following antibodies: anti-GAPDH (Cell Signaling Technology, 1:1000), anti-GFP (Cell Signaling Technology ...
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bioRxiv - Neuroscience 2022Quote: ... membranes were incubated in the appropriate horseradish peroxidase (HRP)-conjugated secondary antibodies (FosB anti-rabbit HRP, GAPDH anti-mouse HRP, Cell signaling) at 1:500 for 1 hr at room temperature ...
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bioRxiv - Physiology 2023Quote: Expression of specific proteins in polysomal profiles (Fig. 6) was determined by probing the membrane with antibodies against RsL7a (dilution 1:4000) from Cell Signaling; 4EBP (dilution 1:4000) ...
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bioRxiv - Neuroscience 2022Quote: ... Neurons were then incubated for 60-180 min at RT with HA antibody (rabbit, 1:250, Cell signaling Technology, cat #C29F4) in PBS–GS blocking solution ...
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bioRxiv - Physiology 2022Quote: ... and probed with rabbit anti-c-Src primary antibodies raised against c-Src in its non-phosphorylated (1:2,000; Cell Signaling, UK) or phosphorylated form (1:1,000 ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were reblocked with PBS Blocking Buffer and reprobed overnight at 4°C with anti-Akt antibody (Cell Signaling Technology #9272) diluted 1:1,000 in PBS antibody buffer ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibodies were prepared in 1% BSA in PBS (SHIP1, 1:100 dilution, Cell Signaling Technologies; IBA1, 1:500 dilution, abcam) and sections were incubated overnight in this solution at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... FOS immunostaining was conducted using rabbit anti-phospho-FOS monoclonal antibody (1:1000, Cat # 5348, RRID:AB_10557109; Cell Signaling Technology, MA, U.S.A.). Slices were washed in PBS 6 × 10 min each ...
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bioRxiv - Neuroscience 2022Quote: ... Targets were detected after incubation with a goat anti-rabbit secondary antibody horseradish peroxidase conjugated (1:10,000; Cell Signaling, Cat# 7074P2) for 1 hour and visualized with the chemiluminescence reagent (Immobilon Forte Western HRP Substrate ...
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bioRxiv - Molecular Biology 2022Quote: The following primary antibodies were used in this study: anti-RhoB (#sc-180, Santa Cruz Biotechnology and #63876, Cell Signaling Technology), anti-VE-cadherin (#2500 ...
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bioRxiv - Microbiology 2022Quote: ... After washing 3 times cells were blocked with 2% BSA for 1h at 37 followed by incubation with 1/100 dilution of anti-Flag monoclonal antibody (Cell Signaling) for 1.5h at RT ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were incubated with primary antibodies for 30 minutes - 1:100 Acetyl-Histone H3 (Lys27) Rabbit mAb (Cell Signaling Technology #8173) or 1:250 Acetyl-Histone H3 (Lys9 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... membranes were incubated with the following primary antibodies in the blocking buffer: rabbit anti-GAPDH (1:2,500; Cell Signaling Technology #2118) and mouse anti-HiBiT (1.0 µg/ml ...
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bioRxiv - Neuroscience 2022Quote: ... Any APP antibodies remaining on the cell surface were quenched with a non-florescent goat anti-mouse secondary antibody conjugated to horseradish peroxidase (Cell Signaling) diluted in 10% goat serum (Invitrogen ...
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bioRxiv - Physiology 2022Quote: ... GAPDH-HRP (GTX627408-01, 1:120000) and P84 (GTX70220-01, 1:1000) The secondary antibodies were Anti-rabbit IgG (Cell Signaling, 7074 ...
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bioRxiv - Cell Biology 2022Quote: The following primary antibodies and concentrations were utilized: rabbit anti-Vimentin (Cell Signaling Technology, D21H3, WB 1:1000, IF 1:200), mouse anti-Vimentin (Invitrogen ...
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bioRxiv - Cancer Biology 2022Quote: ... The secondary antibodies were as follows: horseradish peroxidase (HRP)-conjugated goat anti-mouse immunoglobulin (IgG) (1:10,000, 7076, Cell Signaling Technology), HRP-conjugated goat anti-rabbit IgG (1:10,000 ...
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bioRxiv - Cancer Biology 2022Quote: ... The washed cells were then resuspended in 200 μL of PBS containing 1% BSA and 0.1% Triton X-100 (PBSTx/BSA) containing primary antibodies [MCM2 (1:200, D7G11, Cell Signaling Technology) and ψH2AX (1:200 ...
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bioRxiv - Cell Biology 2022Quote: ... The membrane was blocked with 5% non-fat dry milk in TBST and incubated with primary antibodies against Perilipin-2 (1:5000 gift from Dr. Ronal Kuhnlein) 13 and Actin (1:1000, Cell Signaling). The western blot images were captured by a BIO-RAD workstation.
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bioRxiv - Immunology 2024Quote: ... Primary antibody was removed by several washes with TBS + 0.1% Tween-20 and then subjected to incubation with secondary antibody (anti-mouse-HRP, 7076S, Cell Signaling Technology). The detection of protein bands was then carried out using enhanced chemiluminescence (ECL ...
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bioRxiv - Neuroscience 2023Quote: ... a secondary reaction was performed by simultaneously incubating the sections with a rabbit antibody directly conjugated to Alexa Fluor 488 (1:500; catalog #4412S, Cell Signaling) for CR and with a guinea pig antibody directly conjugated to Alexa Fluor 647 (1:500 ...
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bioRxiv - Neuroscience 2023Quote: ... resolved on SDS-polyacrylamide gel electrophoresis and detected by Western blotting with the following antibodies: rabbit anti-tau (D1M9X; 1:1000, Cell Signaling), mouse anti-tau (TG5 ...
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bioRxiv - Developmental Biology 2024Quote: ... Primary antibodies used in this study are: anti-non-phospho(Ser33/37/Thr41) (active)-CTNNB1 (Cell Signaling Technology, 8814, 1:1,000), anti-(total ...
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bioRxiv - Microbiology 2023Quote: ... TMPRSS2 expression was evaluated using SimpleWestern Abby (ProteinSimple, San Jose, CA, USA) with an anti-Myc tag mouse monoclonal antibody (Cell Signaling Biotechnology ...
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bioRxiv - Physiology 2024Quote: ... the sections were incubated in secondary antibody (1:500, Alexa 488 or 594 Goat Antirabbit; Cell Signaling Technologies, Danvers, MA, USA) for 2 h at room temperature ...
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bioRxiv - Physiology 2024Quote: ... resuspended in 200 μl of AbA with primary antibody for anti-pan actin (dilution of 1:1000, 4968S, Cell Signaling Technology) with rocking in cold room for overnight ...
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bioRxiv - Cell Biology 2024Quote: ... the samples were blocked with 3% BSA in PBS overnight at 4°C and then incubated with a rabbit phospho-SMAD1/5/9 antibody at 1:400 (D5B10, Cell Signaling) and a mouse anti-YAP antibody at 1:200 (63.7 ...
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bioRxiv - Cell Biology 2024Quote: ... solution one hour at room temperature and subsequently incubated overnight 4°C with primary antibodies: anti-S6 (1:10,000; 5G10 monoclonal rabbit; Cell Signaling 2217), anti-Phospho-S6 (1:10,000 ...
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bioRxiv - Cell Biology 2024Quote: ... cells were incubated overnight at 40C with one or more of the following antibodies: rabbit anti-pMLC (ser19) (Cat# 3675, Cell Signaling), rabbit anti-paxillin (Cat# PA534910 ...
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bioRxiv - Molecular Biology 2024Quote: ... IP was performed overnight at 4° C using 5 μg antibody (anti-H3K9me3 Diagenode, ref. C15410056; anti-HA.11 BioLegend ref: 901503; anti-H3K4me3 Cell Signaling, ref ...
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bioRxiv - Neuroscience 2024Quote: ... HA and Myc tag wer detected using anti-HA (see above) an anti-Myc monoclonal antibody (Cell Signaling Technology; Cat# 92013S) at 0.035µg/mL ...
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bioRxiv - Neuroscience 2024Quote: ... Staining with anti-Pu.1 antibody in 1X Permeabilization buffer (microglia marker 1:50, Pu.1-AlexaFluor 647, 9G7 Cell Signaling) was performed for 40 min ...