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Citations for Cell Signaling Technology :
6501 - 6550 of 10000+ citations for 7 CHLORO 1 METHYL 1H PYRAZOLO 4 3 B PYRIDINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2019Quote: ... anti-histone H3 (Cat #4499P, 1:1000 dilution), and anti-histone H4 (Cat #13919P, 1:1000 dilution) were from Cell Signaling. β-actin antibody was from Sigma (Cat #A5441 ...
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bioRxiv - Neuroscience 2019Quote: ... Then the slices were incubated for 24 h at 4°C with primary antibodies, anti-mouse TH (dilution 1:1000, M318, millipore) and anti-rabbit c-Fos (dilution 1:200, 2250, Cell Signaling). The secondary antibodies ...
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bioRxiv - Synthetic Biology 2019Quote: ... Blots were incubated with primary antibodies (HA-Tag #2367, dilution 1:1000; myc-Tag #2276, dilution 1:1000; Cell Signaling Technology; Vincullin ab129002 ...
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bioRxiv - Cell Biology 2020Quote: ... which was performed as previously described (27,28) using the following antibodies: rabbit monoclonal anti-Caveolin-1 (1:100, Cell Signaling #3267); mouse monoclonal anti-Glutamine Synthetase (for Müller glia-specific marker ...
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bioRxiv - Cancer Biology 2020Quote: ... anti-β-catenin Ab (1:500) and anti-p-p53 (phosphorylated on S20) Ab (1:500) were purchased from Cell Signaling. Mouse anti-CA9 Ab (M75 ...
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bioRxiv - Neuroscience 2020Quote: ... The next day sections were washed in PBS and incubated for 1 h with A647-conjugated secondary goat anti-rabbit antibody (1:500, Cell Signaling). Sections were then mounted and cover-slipped.
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bioRxiv - Cell Biology 2020Quote: ... Cell pellets were loosed and resuspended by flick in 100 μl 1% BSA/PBS with 1 μl Alexa Fluor 488 conjugated phospho-Histone H3 (Ser10) antibody (DC28, Cell Signaling). After incubation at room temperature for 1 hour with occasional flick ...
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bioRxiv - Neuroscience 2019Quote: ... and/or immunoblotting (IB) at the indicated dilutions: Rabbit anti-peIF2a (for PLA 1:6000 #44728G Invitrogen, and IB, 1:1000, Cell Signaling), mouse anti-eIF2a (IB ...
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bioRxiv - Cancer Biology 2020Quote: ... For primary antibodies against [p] ERK (diluted 1:500) and total ERK (tERK; diluted 1:500; Cell Signaling Technology, Danvers, MA), antibodies were diluted in 0.1% TBST with 5% BSA ...
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bioRxiv - Cell Biology 2021Quote: ... 1:100 for Cut&Tag), DDX5 (9877T, 1:1,000 for WB), and RBBP5 (13171S, 1:200 for ChIP) from Cell Signaling Technology; PCBP2 (NBP2-19715 ...
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bioRxiv - Cell Biology 2021Quote: ... Primary antibodies used in the study were: anti-acetylated lysine (PTM Biolabs, 1:1000, and Cell Signaling Technology #9441, 1:1000), anti-malonylated lysine (PTM Biolabs ...
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bioRxiv - Biophysics 2020Quote: ... Immunoprecipitates were analyzed by Western blot as described above using the following antibodies: anti-HA rabbit polyclonal (1:1000) and anti-Shh rabbit monoclonal (1:1000, Cell signaling).
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Patient-associated mutations in Drosophila Alk perturb neuronal differentiation and promote survivalbioRxiv - Cancer Biology 2022Quote: ... Primary antibodies used: gp-anti-Alk (1:3000) (Loren et al., 2001) and rb anti-tubulin (1:5000; Cell Signaling, #11H10). After washing ...
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bioRxiv - Cell Biology 2022Quote: ... cells were incubated with the following primary antibodies overnight: rabbit anti-RAD18 NB100-61063 (Novus) (1:200) and mouse anti-biotin (D5A7) (1:200) (Cell signaling). The rest of the protocol follows Proximity Ligation Assay as above.
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bioRxiv - Cell Biology 2021Quote: ... AMPK and pAMPK were stained using primary antibody polyclonal AMPK (1:1000, 2532, from cell signaling) and anti-phosphorylated AMPK (Thr 172) (1:1000, 2535, Cell Signaling), β-actin (1:2000 ...
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bioRxiv - Neuroscience 2021Quote: ... goat anti-chicken 488 (Life Technology A11039 at 1/200) and goat anti-rabbit 488 (Cell Signaling Technology #4412 1/200).
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bioRxiv - Neuroscience 2022Quote: ... rabbit anti-HA tag (C29F4) monoclonal antibody (1:1000 for NaV1.6 and 1:2000 for NF186; Cell Signaling, cat. no. 3724); mouse anti-ankyrin G antibody (1:50 ...
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bioRxiv - Pathology 2022Quote: ... Then membranes were incubated with peroxidase-conjugated secondary antibodies for 1 h at room temperature: anti-rabbit immunoglobulin G (IgG) (AB_2099233; Cat.#7074, 1:2000, Cell Signaling Technology), anti-mouse IgG (AB_330924 ...
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bioRxiv - Developmental Biology 2022Quote: Larval wing imaginal discs were stained using the following antibodies: Rabbit anti-cleaved Drosophila Dcp-1 (1:300, Cell Signaling Technology), Rabbit-anti-p-eIF2α (1:300 ...
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bioRxiv - Neuroscience 2019Quote: ... Primary antibodies were incubated overnight at room temperature (1/100 rat anti-BrdU OBT0030 AbD Serotec; 1/400 rabbit anti-Caspase #9661 Cell Signaling; 1/250 rabbit anti-OTX2 ab92326 Abcam ...
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bioRxiv - Developmental Biology 2019Quote: ... mouse anti-Isl1/2 (Developmental Studies Hybridoma Bank, 1:100) and rabbit anti-β3-tubulin (Cell Signaling Technology, 5568S 1:200).
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bioRxiv - Immunology 2019Quote: ... The concentrated supernatant was mixed with SDS loading buffer for western blotting analysis with antibodies for detection of activated caspase-1 (D5782 1:500; Cell Signaling) or mature IL-1β (Asp116 1:500 ...
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bioRxiv - Neuroscience 2020Quote: ... including the primary antibodies (mouse anti-Iba1 antibody, Abcam, 1:400; rabbit anti-iNOS antibody, Abcam, 1:50; mouse anti-GFAP antibody, Cell signaling, 1:400 ...
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bioRxiv - Synthetic Biology 2021Quote: ... and incubated in 5% non-fat dry milk in 1× PBS and 0.1% Tween 20 with the primary antibodies (PHC1 Cell Signaling #13505 1:1000; Rybp Cell Signaling D8J7W 1:1000 ...
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bioRxiv - Microbiology 2020Quote: ... Nanobodies were incubated for 1 h at RT and detected using Mouse-anti-Myc antibody (1:1000, 9B11 clone, Cell Signaling). US28 was visualized with the rabbit-anti-US28 antibody (1:1000 ...
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bioRxiv - Immunology 2020Quote: ... The drying product was dissolved in 100 μl PBS and mixed with SDS loading buffer for western blotting analysis with antibodies for detection of activated caspase-1 (D5782 1:500, Cell Signaling), mature IL-1β (Asp116 1:500 ...
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bioRxiv - Microbiology 2019Quote: ... and then incubated for 1 h with 1:1,000 dilution of secondary HRP-conjugated goat anti-rabbit IgG antibody (Cell Signaling Technology). Images of the immunoblots were developed with ECLTM Western Blotting Detection Reagent (GE Healthcare).
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bioRxiv - Developmental Biology 2021Quote: ... and 0.05% Tween 20] plus 5% BSA before incubation with the primary antibody (1:1000 for mouse monoclonal 12C11 anti-REST and 1:2000 for anti-LaminB1 rabbit polyclonal, Cell Signaling). Anti-rabbit IgG or anti-mouse IgG conjugated to horseradish peroxidase (1:2000 ...
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bioRxiv - Cell Biology 2019Quote: ... which was used at a 1:50 dilution in combination with a secondary anti-mouse HRP antibody (1:10,000, Cell Signaling #7076S). ECL Select (GE Healthcare ...
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bioRxiv - Bioengineering 2020Quote: ... HRP conjugated goat anti-mouse (1:4000) and goat anti-rabbit (1:4000) secondary antibodies were obtained from Cell Signaling Technology, Danvers ...
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bioRxiv - Cell Biology 2019Quote: ... anti-Mena (1:300, mouse monoclonal, a kind gift from Frank Gertler) and anti-Cre Recombinase (1:300, Cell Signaling 12830). Secondary antibodies used were goat anti-mouse or anti-rabbit IgG conjugated to Alexa Fluor-488 ...
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bioRxiv - Physiology 2021Quote: ... Primary antibodies against p-Akt Ser473 (1:100 dilution; 4060) and p-AMPK Thr172 (1:200 dilution; 50081) from Cell Signaling were attempted ...
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bioRxiv - Physiology 2021Quote: ... anti-phospho and total IkB kinase antibody (p/total IkB 1:1000, 1:500, CST9246S, CST924, Cell Signaling Technology, Danvers, MA), anti-phospho and total panTrk (1:1000 ...
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bioRxiv - Microbiology 2021Quote: ... and 1:1000 dilution for c-Myc(c-Myc(9E10) Santacruz) and 1:1000 dilution for both Cek1/Mkc1(9102S Cell Signaling) and 1:1500 dilution for both phospho-Cek1/phosphor-Mkc1(4307T Cell Signaling) ...
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bioRxiv - Neuroscience 2022Quote: ... Then the sections were incubated with primary antibody of interest in the blockage solution (c-fos,1:200, Abcam/ab208942; NFκB-p65, 1:200, Cell Signaling Technology/8242 ...
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bioRxiv - Physiology 2022Quote: ... GAPDH-HRP (GTX627408-01, 1:120000) and P84 (GTX70220-01, 1:1000) The secondary antibodies were Anti-rabbit IgG (Cell Signaling, 7074 ...
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bioRxiv - Cell Biology 2022Quote: The following primary antibodies and concentrations were utilized: rabbit anti-Vimentin (Cell Signaling Technology, D21H3, WB 1:1000, IF 1:200), mouse anti-Vimentin (Invitrogen ...
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bioRxiv - Cancer Biology 2022Quote: ... The washed cells were then resuspended in 200 μL of PBS containing 1% BSA and 0.1% Triton X-100 (PBSTx/BSA) containing primary antibodies [MCM2 (1:200, D7G11, Cell Signaling Technology) and ψH2AX (1:200 ...
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bioRxiv - Cell Biology 2022Quote: ... The membrane was blocked with 5% non-fat dry milk in TBST and incubated with primary antibodies against Perilipin-2 (1:5000 gift from Dr. Ronal Kuhnlein) 13 and Actin (1:1000, Cell Signaling). The western blot images were captured by a BIO-RAD workstation.
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bioRxiv - Cancer Biology 2024Quote: ... Primary antibodies include PKM1 and PKM2 (7067S, dilution 1:500, and 4053S, dilution 1:1000, respectively, Cell Signaling Technology, Danvers, MA), p63 and FOXA2 (ab735 and ab108422 ...
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bioRxiv - Cell Biology 2024Quote: ... following incubation with the primary antibodies anti-p53 (1:500, mouse, (Ab-6) Pantropic OP43 Sigma-Aldrich) and anti-GAPDH (1:1000, rabbit, 14C10 Cell Signaling) for 1h at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... Respective expression was assessed by immunostaining using a mouse anti-HA antibody (1:500; Cell Signaling#2367, RRID AB_10691311 and a rabbit anti-Flag (1:500; Cell Signaling#14793 RRID AB_2572291 followed by Cy5-conjugated donkey anti-mouse (1.9 µg/ml ...
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bioRxiv - Neuroscience 2023Quote: ... Neurons were then incubated for 1 h with either the rabbit anti-HA antibody (1:500; Cell Signaling #3724, RRID AB_1549585) or the mouse anti-myc antibody (1:500 ...
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bioRxiv - Physiology 2023Quote: ... Secondary antibodies were diluted in 5% blocking solution and membranes were incubated for 1 h at room temperature (1:1000 donkey anti-rabbit IgG, HRP-linked Antibody, #7074S, Cell Signaling). Proteins were detected based on HRP substrate-based enhanced chemiluminescence (#WBKLS0500 ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were blocked with PBS-0.1% Tween 20 (PBS-T) containing 5% milk and were incubated with rabbit monoclonal anti-CYLD (1:500, #8462 Cell Signaling) and mouse monoclonal anti-β-actin (1:5,000 ...
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bioRxiv - Molecular Biology 2023Quote: ... blotted onto nitrocellulose membranes, and probed with anti-ZSWIM8 (1:400; Cat #PA5-59492, Thermo) or anti-GAPDH (1:1000; Cat #2118, Cell Signaling) followed by HRP-conjugated anti-rabbit secondary (1:10,000 ...
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bioRxiv - Cancer Biology 2023Quote: ... phospho-H2AX (S139) (20E3) rabbit mAb (1:400 for standard immunofluorescence, 1:100 for FFPE tissue staining, Cell Signaling Technology #9718); p21 (12D1 ...
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bioRxiv - Developmental Biology 2023Quote: ... Blocking solution was replaced with 1° antibody (Ab) (Phospho-Smad1/5/9 Rabbit mAb #13820, Cell Signaling Technology 13820T, 1:300) in 10%NGS/PBS-Tx and the slides were incubated overnight at 4°C ...
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bioRxiv - Immunology 2023Quote: ... Membranes were blocked with TBS containing 1 % casein and 2 % BSA followed by incubation with primary antibodies (survivin 1:2000, 71G4B7, Cell Signaling; beta actin 1:5000 sc-4778 ...
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bioRxiv - Microbiology 2023Quote: ... A competitor solution was prepared by mixing soluble human IgG1-Fc fused Eph receptor/s (150 nM sEphB2-Fc or 1 μM sEphB3-Fc plus 1μM sEphB4-Fc) and anti-myc Alexa 647 (clone 9B11, 1/250 dilution, Cell Signaling Technology) in PBS-BSA ...