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• The loss of antioxidant activities impairs intestinal epithelium homeostasis by altering lipid metabolism

  Javier Ramos-León, et al., bioRxiv - Molecular Biology 2023

  Quote: ... anti-HGMCS2 (1:100; Cat # 20940, Cell Signaling Technology, USA), anti-ATGL (1:100 ...
• The loss of antioxidant activities impairs intestinal epithelium homeostasis by altering lipid metabolism

  Javier Ramos-León, et al., bioRxiv - Molecular Biology 2023

  Quote: ... anti-Olfm4 (1:100; Cat # 39141, Cell Signaling Technology, USA), anti-HGMCS2 (1:100 ...
• Cytotrophoblast cells are selectively permissive and favor Zika virus, but no other related flavivirus, invasion to the placental stroma

  Mercedes Viettri, et al., bioRxiv - Microbiology 2023

  Quote: ... The following primary antibodies were used: a rabbit Mab S6 ribosomal protein (Cell Signaling®: 2217) and a rabbit Mab Phospho-S6 ribosomal protein (Ser235/236 ...
• GSE1 links the HDAC1/CoREST co-repressor complex to DNA damage

  Terezia Vcelkova, et al., bioRxiv - Molecular Biology 2023

  Quote: ... using the PTMScan Acetyl-Lysine Motif Kit (Cell Signaling Technology, 11663V1). The flow-through of the Acetyl-Lysine immunoprecipitation was kept for phosphorylated peptide enrichment ...
• Su(var)3-9 mediates age-dependent increase in H3K9 methylation on TDP-43 promoter triggering neurodegeneration

  Marta Marzullo, et al., bioRxiv - Molecular Biology 2023

  Quote: ... all diluted in TBS-T: anti-p-p53 (Ser 15; 1:1000, Santa Cell Signaling), anti-p53 (1:1000 ...
• The Respiratory Syncytial Virus M2-2 protein is targeted for proteasome degradation and inhibits translation and stress granules assembly

  Orlando Bonito Scudero, et al., bioRxiv - Microbiology 2023

  Quote: ... anti-Ubiquitin rabbit (#3369 – Cell Signaling, 1:1000 WB), anti-Puromycin mouse (MABE343 – Sigma ...
• The Respiratory Syncytial Virus M2-2 protein is targeted for proteasome degradation and inhibits translation and stress granules assembly

  Orlando Bonito Scudero, et al., bioRxiv - Microbiology 2023

  Quote: ... anti-Phospho-eiF2α rabbit (#3398 – Cell Signaling, 1:1000 WB), anti-eiF2α rabbit (#5324 – Cell Signaling ...
• The Respiratory Syncytial Virus M2-2 protein is targeted for proteasome degradation and inhibits translation and stress granules assembly

  Orlando Bonito Scudero, et al., bioRxiv - Microbiology 2023

  Quote: ... anti-4E-BP1 rabbit (#9452 – Cell Signaling, 1:1000 WB), anti-Mouse HRP (ab97023 – Abcam ...
• Early mitochondrial dysfunction proceeds neuroinflammation, synaptic alteration, and autophagy impairment in hippocampus of App knock-in Alzheimer mouse models

  Luana Naia, et al., bioRxiv - Neuroscience 2023

  Quote: ... ULK1 (1:500) (Cell signaling, Cat. no. 8054), pSer555-ULK1 (1:500 ...
• Early mitochondrial dysfunction proceeds neuroinflammation, synaptic alteration, and autophagy impairment in hippocampus of App knock-in Alzheimer mouse models

  Luana Naia, et al., bioRxiv - Neuroscience 2023

  Quote: ... pSer757-ULK1 (1:500) (Cell Signaling, Cat. no. 14202), β3 tubulin (1:2,000 ...
• Early mitochondrial dysfunction proceeds neuroinflammation, synaptic alteration, and autophagy impairment in hippocampus of App knock-in Alzheimer mouse models

  Luana Naia, et al., bioRxiv - Neuroscience 2023

  Quote: ... pSer555-ULK1 (1:500) (Cell Signaling, Cat. no. 5869), pSer757-ULK1 (1:500 ...
• Continued dysfunction of capillary pericytes promotes no-reflow after experimental stroke in vivo

  J Shrouder, et al., bioRxiv - Neuroscience 2023

  Quote: ... sections were incubated with primary antibody buffer with 1:200 rabbit anti-Ki67 (D3B5, Cell Signaling) for 2 days on a rotary shaker at 4°C ...
• Zika virus infection induces IL-1β-mediated inflammatory responses by macrophages in the brain of an adult mouse model

  Gi Uk Jeong, et al., bioRxiv - Microbiology 2023

  Quote: ... anti-p44/42 MAPK (9102S, Cell Signaling Technology), anti-phospho-p44/42 MAPK (4370S ...
• Ehrlichia Notch signaling induction promotes XIAP stability and inhibits apoptosis

  LaNisha L. Patterson, et al., bioRxiv - Microbiology 2023

  Quote: ... XIAP and NICD antibodies (Cell Signaling Technology) were used to determine interactions ...
• Ehrlichia Notch signaling induction promotes XIAP stability and inhibits apoptosis

  LaNisha L. Patterson, et al., bioRxiv - Microbiology 2023

  Quote: ... rabbit monoclonal α-Caspase-7 (9494S; Cell Signaling Technology), rabbit monoclonal α-Caspase-8 (4790T ...
• Zika virus infection induces IL-1β-mediated inflammatory responses by macrophages in the brain of an adult mouse model

  Gi Uk Jeong, et al., bioRxiv - Microbiology 2023

  Quote: ... anti-phospho-p38 MAPK (9211S, Cell Signaling Technology), anti-p44/42 MAPK (9102S ...
• Zika virus infection induces IL-1β-mediated inflammatory responses by macrophages in the brain of an adult mouse model

  Gi Uk Jeong, et al., bioRxiv - Microbiology 2023

  Quote: ... anti-C/EBP-β (90081S, Cell Signaling Technology), anti-C/EBP-β (LAP ...
• Ehrlichia Notch signaling induction promotes XIAP stability and inhibits apoptosis

  LaNisha L. Patterson, et al., bioRxiv - Microbiology 2023

  Quote: ... and Staurosporine (9953S; Cell Signaling Technology).
• Zika virus infection induces IL-1β-mediated inflammatory responses by macrophages in the brain of an adult mouse model

  Gi Uk Jeong, et al., bioRxiv - Microbiology 2023

  Quote: ... anti-phospho-C/EBP-β (Thr235) (3084S, Cell Signaling Technology), anti-p38 MAPK (9212S ...
• Gas6 ameliorates intestinal mucosal immunosenescence to prevent the translocation of a gut pathobiont, Klebsiella pneumoniae, to the liver

  Hitoshi Tsugawa, et al., bioRxiv - Microbiology 2023

  Quote: ... and anti-GAPDH (Cell Signaling Technologies, Danvers, MA, USA, cat# 2118S). The following antibodies were used for flow cytometry analysis ...
• Multimodal HLA-I genotype regulation by human cytomegalovirus US10 and resulting surface patterning

  Carolin Gerke, et al., bioRxiv - Microbiology 2023

  Quote: ... Incubation with specific antibodies was followed by peroxidase-conjugated secondary antibodies and detection using SignalFire ECL Reagent (Cell Signaling Technology) and the ChemiDoc XRS System (Bio-Rad).
• In vitro production of cat-restricted Toxoplasma pre-sexual stages by epigenetic reprogramming

  Ana Vera Antunes, et al., bioRxiv - Microbiology 2023

  Quote: ... rabbit anti-FLAG (Cell Signaling Technology, RRID: AB_2798687), mouse anti-MYC clone 9B11 (RRID ...
• Long-read sequencing and profiling of RNA-binding proteins reveals the pathogenic mechanism of aberrant splicing of an SCN1A poison exon in epilepsy

  Hannah C Happ, et al., bioRxiv - Genetics 2023

  Quote: ... Beta-actin (1:1000, Cell Signaling Technologies #3700S) was also used as additional sample loading control ...
• Regulation of oncogene-induced senescence by the MRE11 and TREX1 nucleases

  Hervé Técher, et al., bioRxiv - Molecular Biology 2023

  Quote: ... Then were processed with commercial SA-β-galactosidase staining kits following provider instructions (Kit #9860 from Cell signaling).
• Regulation of oncogene-induced senescence by the MRE11 and TREX1 nucleases

  Hervé Técher, et al., bioRxiv - Molecular Biology 2023

  Quote: ... anti-cGAS (1/500, Cell Signaling, #15102), anti-p53 (1/500 ...
• Regulation of oncogene-induced senescence by the MRE11 and TREX1 nucleases

  Hervé Técher, et al., bioRxiv - Molecular Biology 2023

  Quote: ... Immunostaining was performed overnight at 4°C with rabbit anti-cGAS D1D3G (1:400, Cell Signaling, #15102) and secondary goat anti-rabbit coupled to Alexa 546 (1:500 ...
• A monoclonal antibody raised against human EZH2 cross-reacts with the RNA-binding protein SAFB

  Rachel E. Cherney, et al., bioRxiv - Molecular Biology 2023

  Quote: ... 10μL EZH2 antibody (Cell Signaling #5246) per 10 million cells was added ...
• High-resolution structure of mammalian PI31–20S proteasome complex reveals mechanism of proteasome inhibition

  Hao-Chi Hsu, et al., bioRxiv - Molecular Biology 2023

  Quote: ... Rabbit polyclonal antibody against human β tubulin was purchased from Cell Signaling Technology (2146) and used at 1:1000 dilution ...
• A monoclonal antibody raised against human EZH2 cross-reacts with the RNA-binding protein SAFB

  Rachel E. Cherney, et al., bioRxiv - Molecular Biology 2023

  Quote: ... were washed 3 times in blocking buffer (0.5% BSA in 1xPBS) and incubated overnight at 4°C with 7.5μL antibody (anti-EZH2, Cell Signaling #5246 ...
• A monoclonal antibody raised against human EZH2 cross-reacts with the RNA-binding protein SAFB

  Rachel E. Cherney, et al., bioRxiv - Molecular Biology 2023

  Quote: ... and incubated overnight at 4°C with 7.5μL antibody (anti-EZH2, Cell Signaling #5246; SAFB, Bethyl A300-812A; SUZ12, Cell Signaling #3737) or 3μL (7.5μg ...
• A monoclonal antibody raised against human EZH2 cross-reacts with the RNA-binding protein SAFB

  Rachel E. Cherney, et al., bioRxiv - Molecular Biology 2023

  Quote: ... were washed 3 times in blocking buffer (0.5% BSA in 1xPBS) and incubated overnight at 4°C with 20μL EZH2 antibody (anti-EZH2, Cell Signaling #5246) or 20μg IgG (anti-IgG ...
• A monoclonal antibody raised against human EZH2 cross-reacts with the RNA-binding protein SAFB

  Rachel E. Cherney, et al., bioRxiv - Molecular Biology 2023

  Quote: ... were washed three times in blocking buffer (0.5% BSA in 1xPBS) and incubated overnight at 4°C with 10μL antibody (anti-SAFB, Bethyl 812-300A; EZH2, Cell Signaling #5246) or 10μg IgG (Invitrogen #02-6502) ...
• A monoclonal antibody raised against human EZH2 cross-reacts with the RNA-binding protein SAFB

  Rachel E. Cherney, et al., bioRxiv - Molecular Biology 2023

  Quote: ... EZH2 (Cell Signaling #3147, 1:1000), SAFB (Bethyl A300-812A ...
• Resistance Training Diminishes Mitochondrial Adaptations to Subsequent Endurance Training

  Paulo H. C. Mesquita, et al., bioRxiv - Molecular Biology 2023

  Quote: ... or anti-mouse IgG (Cell Signaling Technology Cat# 7076, RRID:AB_330924). Membranes were then washed in TBST ...
• A role for N6-methyldeoxyadenosine in C. elegans mitochondrial genome regulation

  Lantana K. Grub, et al., bioRxiv - Genetics 2023

  Quote: ... 1:2000 Goat anti-Rabbit IgG HRP-linked (Cell Signaling, 7074S), for 1 hr at room temperature ...
• A role for N6-methyldeoxyadenosine in C. elegans mitochondrial genome regulation

  Lantana K. Grub, et al., bioRxiv - Genetics 2023

  Quote: ... or control IgG (Cell Signaling, 2729S) was diluted in 200 µL PBS supplemented with 0.02% Tween ...
• Oncogenic reactivation of young L1s is a hallmark of colon cancer

  Devin Neu, et al., bioRxiv - Genomics 2023

  Quote: ... Membranes were then washed with PBST and incubated with HRP-conjugated secondary antibodies (Santa Cruz Biotechnology Inc., catalog sc-2004; Cell Signaling Technology, catalog 7076S) at room temperature for 1 hour ...
• Resistance Training Diminishes Mitochondrial Adaptations to Subsequent Endurance Training

  Paulo H. C. Mesquita, et al., bioRxiv - Molecular Biology 2023

  Quote: ... membranes were washed three times in TBST for 5 minutes and incubated for 1 hour with horseradish peroxidase-conjugated anti-rabbit IgG (Cell Signaling Technology Cat# 7074, RRID:AB_2099233) or anti-mouse IgG (Cell Signaling Technology Cat# 7076 ...
• Resistance Training Diminishes Mitochondrial Adaptations to Subsequent Endurance Training

  Paulo H. C. Mesquita, et al., bioRxiv - Molecular Biology 2023

  Quote: ... MFN1 (Cell Signaling Technology Cat# 14739, RRID:AB_2744531), MFN2 (BioVision Cat# 3882– 100 ...
• Resistance Training Diminishes Mitochondrial Adaptations to Subsequent Endurance Training

  Paulo H. C. Mesquita, et al., bioRxiv - Molecular Biology 2023

  Quote: ... and PARKIN (Cell Signaling Technology Cat# 2132, RRID:AB_10693040). Following primary antibody incubations ...
• mRNA capping enzyme exports to cytoplasm, localizes to stress granules and maintains cap homeostasis of the target mRNAs

  Anakshi Gayen, et al., bioRxiv - Molecular Biology 2023

  Quote: ... Cell viability was measured from the cells growing in 96-well plate using XTT assay kit (Cell Signaling Technology) following manufacturer’s instruction using BioTek Synergy microplate reader.
• Mrc1^Claspin is essential for heterochromatin maintenance in Schizosaccharomyces pombe

  Kei Kawakami, et al., bioRxiv - Molecular Biology 2023

  Quote: ... and anti-myc (9B11, Cell signaling Technology, Danvers, MA, USA) were used as primary antibodies ...
• KIBRA repairs synaptic plasticity and promotes resilience to tauopathy-related memory loss

  Grant Kauwe, et al., bioRxiv - Neuroscience 2023

  Quote: ... anti-PSD95 (2507, Cell Signaling Technology, RRID: AB_561221), anti-HA (H6908 ...
• YAP and TAZ differentially regulate postnatal cortical progenitor proliferation and astrocyte differentiation

  Jessie Chen, et al., bioRxiv - Neuroscience 2023

  Quote: ... LATS1 (Cell Signaling, 1:1000), pGSK3b (Cell Signaling 9336 ...
• Genetically perturbed myelin as a risk factor for neuroinflammation-driven axon degeneration

  Janos Groh, et al., bioRxiv - Neuroscience 2023

  Quote: ... Free-floating sections were blocked using 5% BSA and 5% donkey serum in PBS containing 0.3% Triton X-100 and incubated overnight at 4 °C with a combination of the following antibodies and stains: rabbit anti-pMLC Thr18/Ser19 (1:100, catalog no. 3674; Cell Signaling); mouse anti- CASPR (1:1000 ...
• YAP and TAZ differentially regulate postnatal cortical progenitor proliferation and astrocyte differentiation

  Jessie Chen, et al., bioRxiv - Neuroscience 2023

  Quote: ... GSK3b (Cell Signaling 9315, 1:500). Secondary antibodies were used at 1:2000 ...
• YAP and TAZ differentially regulate postnatal cortical progenitor proliferation and astrocyte differentiation

  Jessie Chen, et al., bioRxiv - Neuroscience 2023

  Quote: ... TAZ (Cell Signaling 83669S, 1:500), pTAZ (CS 59971 ...
• Mitochondrial DNA released by senescent cells triggers immunosuppression in cancer

  Ping Lai, et al., bioRxiv - Cell Biology 2023

  Quote: ... Senescence-associated SA-β-gal staining was performed using Senescence β-Galactosidase Staining Kit (Cell Signaling Technology, 9860) according to the manufacturer’s instructions ...
• Genetically perturbed myelin as a risk factor for neuroinflammation-driven axon degeneration

  Janos Groh, et al., bioRxiv - Neuroscience 2023

  Quote: ... rabbit anti-cleaved caspase 3 (1:300, catalog no. 9664; Cell Signaling); rabbit anti-pMLC Thr18/Ser19 (1:100 ...
• Germ granule association drives small RNA specificity for a nuclear Argonaute protein

  Shihui Chen, Carolyn M. Phillips, bioRxiv - Molecular Biology 2023

  Quote: ... anti-H3K27me3 antibody (Cell Signaling 9733T) was used at 1:50 for positive control ...