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Citations for Cell Signaling Technology :
551 - 600 of 1703 citations for 4 4 Methylphenyl 1H pyrrole 3 carbonitrile since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... the cells were blocked with 0.2% bovine serum albumin and incubated overnight at 4°C with the anti-EEA (1:200; Cell Signaling Technology, 3288) or anti-LAMP1 (1:200 ...
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bioRxiv - Cell Biology 2024Quote: ... The membrane was then incubated under soft agitation overnight at 4°C with the primary antibodies for DNMT1 (Cell Signaling Technology, 5032) and β-actin (Cell Signaling Technology ...
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bioRxiv - Microbiology 2024Quote: ... Samples containing 10 μg of DNA were then incubated overnight at 4°C with either anti-RNA pol II antibody (mouse anti-Rpb1 CTD, Cell Signaling, 2629S), mouse anti-SP1 antibody (Santa Cruz Biotechnology ...
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bioRxiv - Genetics 2024Quote: ... The clarified nuclear extracts were incubated with IgG antibody applied as negative control or target antibody shown in Table 2 with rotation overnight at 4°C and conjugated with Chromatin immunoprecipitation-grade protein G magnetic beads (Cell Signaling Technology). After extensive washing ...
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bioRxiv - Cancer Biology 2024Quote: ... The cells were then incubated at 4 °C overnight with the following primary antibodies at 1:100 dilutions: anti-Ikzf1 (rabbit, D6N9Y, Cell Signaling Technology), anti-CD31 (mouse ...
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bioRxiv - Cancer Biology 2024Quote: ... tissue sections were incubated with 5% goat serum in PBS-T for one hour and then incubated at 4°C overnight with the following antibodies: SOX2 (23064, Cell Signaling Technology), Ki67 (ab16667 ...
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bioRxiv - Cancer Biology 2024Quote: ... The blots were then incubated separately with respective primary antibodies for overnight at 4°C [ZEB1 (1:3000) (Cell Signaling Technologies, USA); GPX4 (1:3000 ...
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bioRxiv - Developmental Biology 2024Quote: ... cells were fixed in 4% PFA for 10 minutes and processed for immunostaining by standard methods using combinations of rabbit (Cell Signaling, 71D10) or mouse anti-Myc (Cell Signaling ...
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bioRxiv - Cancer Biology 2020Quote: ... Caspase-3 (14220s) and cleaved caspase-3 (9664s) from Cell Signaling.
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bioRxiv - Biochemistry 2020Quote: ... and pan 14-3-3 loading control (#8312; Cell Signaling Technologies). Detection was achieved with HRP-conjugated donkey anti-rabbit secondary antibody (Jackson ImmunoResearch ...
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bioRxiv - Neuroscience 2020Quote: ... sections were washed in PBS and incubated for 1h with A488-conjugated secondary goat anti-rabbit antibody (1:500, Cell Signaling, USA). Similar procedures were followed for POMC labelling in brain sections from POMC-CreERT2-Ai6 mice ...
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bioRxiv - Cell Biology 2022Quote: ... membranes were incubated with the secondary antibody for 1h at room temperature (rabbit-IgG HRP-linked 1:10000, Cell Signaling Technology (#7074), mouse-IgG HRP-linked 1:10000 ...
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bioRxiv - Developmental Biology 2023Quote: ... Primary antibody incubation was performed for 1h at room temperature in phospho-p44/42 MAPK (ERK1/2) (Thr202/Thr304) antibody (#4370, 1:200, Cell Signaling Technology) or human VE-Cadherin antibody (#AF938 ...
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bioRxiv - Cancer Biology 2023Quote: ... The primary antibody incubation was followed by incubation in HRP-conjugated secondary antibody for 1h RT at room temperature (goat anti mouse IgG and goat anti-rabbit IgG, Cell Signaling Technology). Bands were detected by chemiluminescence using ECL solution (WesternBright Sirius ...
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bioRxiv - Developmental Biology 2023Quote: ... Slides were blocked in 0.5% BR (Blocking Reagent (Akoya Biociences))/TNT (TN, 0.05% Tween) for 1h at RT and incubated with a primary antibody against pSMAD1,5,9 (Cell Signaling 13820S, rabbit, 1:300) overnight at 4°C ...
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bioRxiv - Genomics 2024Quote: ... was added along with Nt.CviPII-pGL enzyme (1 unit per well) for 1h at 37°C or pAG-Mnase (1.5 μl per well, Cell Signaling Technology # 40366S). After 3 washes with NEED-seq washing buffer (20 mM KPO4 ...
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bioRxiv - Biochemistry 2024Quote: ... and incubated for 1h in 100 μL PBST with secondary anti-rabbit IgG (H+L) (Alexa Fluor® 647 Conjugate, Cell Signaling). Stained cells were then washed twice with PBST and resuspended in 300 μL of PBS with 5 μg/mL DAPI to stain cells for DNA content.
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bioRxiv - Genomics 2021Quote: ... Nuclear stain solution was prepared for 4 slides at a time: 20 µLSYTO13 (Nanostring) and 180 µL 1x TBS (Cell Signaling Technologies, 12498S) for a total volume of 200 µL/slide ...
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bioRxiv - Neuroscience 2020Quote: ... were lysed in complete RIP lysis buffer and the cell lysates were divided into equal parts and incubated (overnight with rotation, at 4°C) with anti-Pol II (Cell Signaling Technology (CST), Cat ...
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bioRxiv - Neuroscience 2020Quote: ... The membrane was then probed with the following primary antibodies diluted in 5 % BSA /PBS-T for overnight at 4 °C: β-actin (1:1000; Cell Signaling Technology), ZO-1 (1.25 μg/ml) ...
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bioRxiv - Synthetic Biology 2021Quote: ... Membranes were then incubated overnight at 4°C with rabbit anti-phosphorylated ERK1/2 antibody (Cell Signaling Technology; 9101S; LOT: 30; 1:1,000) in TBST containing 5% (w/v ...
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bioRxiv - Neuroscience 2020Quote: ... The membranes were probed overnight at 4°C using primary antibodies against p-eIF2α S51 (rabbit, Cell Signaling cat no: 9721, 1:500), anti-t-eIF2α (rabbit ...
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bioRxiv - Cancer Biology 2020Quote: ... Samples were then fixed with 4% paraformaldehyde and processed for immunostaining with EGFR (D38B1) XP rabbit mAb (4267, Cell Signaling Technology, 1:100), anti-rabbit secondary antibody Alexa Fluor 555 Conjugate (4413 ...
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bioRxiv - Cell Biology 2020Quote: SMC cultured on glass coverslips were fixed in 4% PFA prior to immunostaining with phospho-histone H2AX (γH2AX) antibody (Cell Signaling Technologies #2577) as described previously (19) ...
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bioRxiv - Cancer Biology 2022Quote: Whole cell protein was extracted from cells treated for 24 h with 1 mM aspirin and 4 μM regorafenib using radio-immunoprecipitation assay buffer (Cell Signaling Cat# 9806) supplemented with 1 mM phenylmethanesulfonyl fluoride (Cell Signaling Cat# 8553) ...
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bioRxiv - Molecular Biology 2022Quote: ... The blocked membrane was incubated overnight at 4°C with primary antibodies diluted in 1x Tris buffered saline with Tween-20 (TBST, Cell Signaling Technology: 9997S). The membrane was washed twice using TBST ...
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bioRxiv - Systems Biology 2022Quote: ... blocked in 5% dry milk (American bioInc) for 60 min followed by incubation overnight (at 4°C) with primary antibody according to manufacturer’s instruction (Cell Signaling Technology, pSMAD3 (9520S) SMAD3 (9513S) ...
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bioRxiv - Biochemistry 2022Quote: ... Membranes were blocked followed by overnight incubation at 4°C with primary antibodies: C-terminal Parkin antibody (PRK8; Cell Signaling, 1:40,000 dilution) and N-terminal Parkin antibody (https://mrcppureagents.dundee.ac.uk/reagents-view-antibodies/589302 ...
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bioRxiv - Neuroscience 2020Quote: ... Slices were then incubated for 48h at 4°C with the following primary antibodies: rabbit anti-phospho-S6 (1:1000, Cell Signaling, Cat# 5364), mouse anti-NeuN (1:400 ...
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bioRxiv - Pathology 2021Quote: ... and incubated with primary antibodies at 4°C overnight at the following concentrations: β-catenin (Cell Signaling, 8480, 1:1000 in 1% BSA), p65 (Cell Signaling ...
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bioRxiv - Cancer Biology 2020Quote: ... for 2 hours and blotted at 4°C overnight with primary antibodies as follows: rabbit anti-phospho-GSK-3β (Ser9) (5B3) (1/500 dilution; Cell Signaling; cat #9323), rabbit anti-GSK-3β (1/1000 dilution ...
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bioRxiv - Cell Biology 2020Quote: ... of embedded organoids or whole-mounted organoids were incubated overnight at 4°C with rabbit monoclonal anti-p63a antibody (D2K8X, Cell Signaling Technology, MA), polyclonal guinea pig anti-cytokeratin 8/18 (03-GP11 ...
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bioRxiv - Cell Biology 2020Quote: ... The membranes were blocked at room temperature with 1% BSA for 1 h and incubated overnight at 4 °C with primary antibodies: rabbit Collagen I antibody (Cell Signaling Technology, 84336), mouse E-cadherin antibody (Abcam ...
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Development of follicular dendritic cells in lymph nodes depends on retinoic acid mediated signalingbioRxiv - Immunology 2020Quote: ... Cells were fixed with 4% paraformaldehyde for 5 min and subsequently permeabilized with 0.2% Triton-X and stained with anti-relB (Cell Signaling Technology, Danvers, USA) followed by goat-anti-rabbit Alexa 546 (Invitrogen) ...
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bioRxiv - Neuroscience 2020Quote: ... Membranes were probed overnight at 4°C in Odyssey Blocking Buffer with antibodies recognizing PS6 (Ser240/244; rabbit monoclonal; 1:1000; Cell Signaling, Danvers, MA), total S6 ribosomal protein (mouse monoclonal ...
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IL-27R signaling serves as immunological checkpoint for NK cells to promote hepatocellular carcinomabioRxiv - Immunology 2020Quote: ... and blocked with 5% skimmed milk in TBST for 1 h followed by O/N incubation at 4 °C with appropriate primary antibody: pSTAT3 (D3A7; Cell Signaling Technology, 9145S) and STAT3 (124H6 ...
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bioRxiv - Developmental Biology 2021Quote: ... Blocked membranes were incubated overnight at 4°C with primary antibodies diluted in blocking solution: rabbit anti-Ezh2 (Cell Signaling Technologies; 1:1000), rabbit anti-H3K27me3 (Millipore ...
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bioRxiv - Neuroscience 2021Quote: ... and incubated at 4°C for 18-22 hr in a cocktail comprising anti-GSK3β mouse monoclonal antibody (1:1500; #9832, Cell Signaling, Rockford, IL), anti-phospho GSK3β (Ser9 ...
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bioRxiv - Neuroscience 2023Quote: ... chemically cross-linked to protein G-agarose beads at 4°C on a mini-rotator. Rabbit anti-GFP (TorryPines, cat. # TP401,) or normal rabbit IgG (Cell signaling, cat. # 2729) chemically cross-linked to protein G agarose beads were used as controls ...
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bioRxiv - Neuroscience 2022Quote: ... 200,000 Bead-bound cells were incubated with primary antibody overnight at 4°C (1:100 Acetyl-Histone H3 (Lys27) Rabbit mAb (Cell Signaling Technology #8173), 1:50 Acetyl-Histone H3 (Lys9 ...
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bioRxiv - Cell Biology 2023Quote: ... cells were incubated overnight at 4°C in 0.2% BSA/TBS with the following antibodies: anti-Myc mouse IgG (Cell Signaling, cat. No. 2276) for mDGKε-Myc staining and anti-STIM1 rabbit IgG (Cell Signaling ...
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bioRxiv - Cell Biology 2024Quote: ... were then added to the membrane and incubated overnight at 4°C: anti-Calnexin (1:1,000 dilution, clone C5C9, Cell Signaling Technology, Danvers, MA), anti-Mitofilin (1:500 dilution ...
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bioRxiv - Cell Biology 2024Quote: ... the membranes were incubated overnight at 4°C with the following primary antibodies: anti-AMPKα (Cell Signaling Technology, USA; diluted at 1:1000), anti-p- AMPKα (Thr172 ...
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bioRxiv - Neuroscience 2024Quote: ... or 5% non-fat dry milk in TBST for 1 h then incubated overnight at 4°C in blocking buffer containing antibodies against TMEM106B (1:500, Cell Signaling Technology 93334), TDP-43 (1:1,500 ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were blocked in 5% non-fat dry milk in TBST for 1 h and then incubated overnight at 4°C in blocking buffer containing the antibody against TMEM106B (1:500, Cell Signaling Technology 93334). The secondary antibody ...
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bioRxiv - Cell Biology 2024Quote: ... All cell pellets were then resuspended in 100 µL fresh MG medium and stained with 1.4 µM 4’,6-diamidino-2-phenylindole (DAPI) from Cell Signaling Technology (Cat #4083S) for 15 min in the dark at 30 °C in the microaerobic chamber ...
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bioRxiv - Molecular Biology 2023Quote: ... The membrane was incubated overnight at 4°C with the primary antibody mAb c-MYC (dilution 1:1000 in TBST/5% milk; Cell Signaling Technology, D84C12). Subsequently ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were then immunoblotted over night at 4°C with the indicated primary antibodies diluted in blocking buffer: Phosphorylated Stat1 (Tyr701) (D4A7) (Cell Signaling, 1:1000), STAT1 (Cell Signaling ...
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bioRxiv - Neuroscience 2024Quote: ... The incubation was performed using the free-floating method at 4°C (48 h) with rabbit anti-p75NTR(1:500; Cell signaling, MA, USA) with 0.6% Triton X-100 in PBS with 5% BSA ...
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bioRxiv - Cell Biology 2022Quote: ... then incubated with hematoxylin-eosin staining for 10min or overnight at 4℃ using the following primary antibodies: β-catenin (1:200, #8480, Cell Signaling Technology), Ki67 (1:400 ...