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Citations for Cell Signaling Technology :
451 - 500 of 1703 citations for 4 4 Methylphenyl 1H pyrrole 3 carbonitrile since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... The next day sections were washed in PBS and incubated for 1h with A647-conjugated secondary goat anti-rabbit antibody (1:500, Cell Signaling).
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bioRxiv - Neuroscience 2020Quote: ... The next day sections were washed in PBS and incubated for 1h with A488-conjugated secondary goat anti-rabbit antibody (1:500, Cell Signaling).
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bioRxiv - Neuroscience 2020Quote: ... The next day sections were washed in PBS and incubated for 1h with A647-conjugated secondary goat anti-rabbit antibody (1:500, Cell Signaling). Sections were washed in PBS and incubated with Goat Fab Fragment Anti-Rabbit IgG (1:100 ...
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bioRxiv - Microbiology 2022Quote: ... After washing 3 times cells were blocked with 2% BSA for 1h at 37 followed by incubation with 1/100 dilution of anti-Flag monoclonal antibody (Cell Signaling) for 1.5h at RT ...
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bioRxiv - Biochemistry 2024Quote: ... Afterwards cells were incubated in blocking solution for 1h at 37 °C and incubated in the primary antibody (Cx36 1:2000, Chemicon, MAB3045; Sec24B 1:1000; Cell Signaling; Grasp55 1:2000 ...
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bioRxiv - Cancer Biology 2022Quote: ... then incubated in HRP-linked anti-mouse or anti-rabbit IgG antibody for 1h at 24°C with gentle rocking (1:5,000 dilution, Cell Signaling Technology). Membranes were washed in TBST and protein bands were visualized using the Pierce ECL detection system (Cat #32106 ...
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bioRxiv - Microbiology 2023Quote: ... and incubated for 1h with primary antibodies (1:4,000 dilution, anti-flag mouse -Sigma #F1804 or anti-myc mouse - Cell Signaling #2276). Membranes were then incubated for 1h with a secondary antibody (anti-mouse HRP conjugate at 1:10,000 dilution -Cell Signaling ...
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were blocked for 1h and probed with 5% nonfat dry milk with the following primary antibodies: anti-IRF8 (clone D20D8, Cat# 5628, Cell Signaling), anti-CD74 (sheep anti-mouse cd74 ...
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bioRxiv - Physiology 2024Quote: Mouse retinae were fixed in 4% formaldehyde in PBS for 1h and wholemount immunostained as described [31] using rabbit anti-P-p38 MAPK (Thr180/Tyr182) antibody (Cell Signaling) followed by Alexa Fluor 555–conjugated goat anti–rabbit antibodies (Invitrogen ...
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bioRxiv - Neuroscience 2024Quote: ... washed in TBST-Tween20 and blocked for 1h at room temperature with TBST/5% Normal Goat Serum (Cell Signaling Cat# 5425). Following blocking ...
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bioRxiv - Cancer Biology 2024Quote: ... the membrane was washed in TBS-T and incubated for 1h with goat anti-rabbit (cat. no. 7074S, Cell Signaling Technology) or goat anti-mouse (cat ...
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bioRxiv - Cell Biology 2024Quote: ... 14-3-3 (pan) E9S9M Rabbit mAb (Cell Signaling Technology), and Purified Mouse Anti-MEK1 Clone 25/MEK1 (BD Biosciences).
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bioRxiv - Genomics 2024Quote: ... SMAD2/3 (SMAD 2/3 XP, Cell Signaling Technology 8685) or isotype control (Rabbit IgG XP ...
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bioRxiv - Bioengineering 2020Quote: ... microchannels were incubated overnight at 4°C with anti-vascular endothelial-cadherin (VE-cadherin) and anti-epithelial cadherin (E-cadherin) (Cell Signaling Technologies) and primary antibodies were diluted with blocking buffer for HUVECs and BeWo cells ...
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bioRxiv - Cancer Biology 2021Quote: ... The membranes were blocked and incubated overnight at 4 °C with a mouse monoclonal anti-FGFR1 antibody (1:100; Cell Signaling Technology), a rabbit polyclonal anti-p-AKT antibody (1:500 ...
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bioRxiv - Cell Biology 2020Quote: ... for 1 h at room temperature followed by incubation overnight at 4°C with a specific primary antibody to p-p53ser15 (Cell Signaling, 9284S), β-actin (Cell Signaling ...
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bioRxiv - Cell Biology 2020Quote: ... Blots were washed 3X for 5 minutes in 1X TBST and incubated overnight at 4°C in 5% BSA w/v in 1X TBST with primary antibodies raised against phospho-S6 (Cell Signaling 4858), S6 (Cell Signaling 2217) ...
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bioRxiv - Cancer Biology 2020Quote: ... for 1 hour and probed overnight at 4°C with the primary antibodies of interest directed against: rabbit anti-BCL-2 (Cell Signaling, CST4223), rabbit anti-BCL-xL (Cell Signaling ...
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bioRxiv - Genetics 2021Quote: ... were incubated overnight at 4 degrees Celcius in a 1:1000 dilution with the following primary antibodies: anti-TP53 (Cell Signaling, 2524), anti-BRCA1 (Abclonal ...
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bioRxiv - Genetics 2021Quote: ... The following primary antibodies were used at 1:1000 dilution in PBST (0.05% Tween-20) and 5% non-fat dry milk and incubated overnight at 4°C: Myc (Cell Signaling Technology #2276), RBPJ (Cell Signaling Technology #5313) ...
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bioRxiv - Developmental Biology 2021Quote: ... cells were fixed in 4% paraformaldehyde and processed for immunostaining by standard methods using mouse anti-Flag (9A3, Cell Signaling, 1:400), rabbit anti-Myc (71D10 ...
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bioRxiv - Molecular Biology 2021Quote: ... and Tween20 (0.02%)) for 20 minutes at RT followed by treatment overnight at 4 °C with anti-γ-H2AX (Cell Signaling #9718S) diluted 1:500 in Ab mix or anti-PCNA diluted 1:1000 (DAKO #M0879) ...
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bioRxiv - Developmental Biology 2021Quote: ... 0.3% Triton X-100), incubated in rabbit anti-phospho-Histone H3-S10 at 1:2000 overnight (O/N, 4°C) (Cell Signaling 3377S), washed 8X in PBSTx (1X PBS plus 0.3% Triton X-100 ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were incubated overnight in blocking buffer at 4°C with the following primary antibodies: 1:2000 c-fos (Cell signaling technology), 1:2000 ds-red (Takara Bio) ...
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bioRxiv - Cancer Biology 2020Quote: ... Blots were incubated with the primary antibodies in TBS 0.05% Tween-20 / 5% milk overnight at 4°C and subsequently incubated with HRP coupled secondary goat anti-rabbit (7074; Cell Signaling, Switzerland) and goat anti-mouse antibody (7076 ...
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bioRxiv - Genetics 2020Quote: ... followed by overnight incubation with primary antibodies overnight at 4°C (anti-dS6K gift from Thomas Neufeld; dilution at 1:3500, anti-pS6KThr398 Cell Signaling Technologies, #9209 ...
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bioRxiv - Cancer Biology 2020Quote: ... cells grown on chamber microscope slides were fixed in 4% paraformaldehyde and treated with immunofluorescence blocking buffer (catalog #12411, Cell Signaling Technology). Staining with primary antibodies against involucrin (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2020Quote: ... blocked using 5% milk in TBST for 1 hour followed by primary antibody incubation at 4°C for overnight using the following antibodies at the appropriate dilutions– LC3 (1:1000, #2775, Cell Signaling Technologies), Atg5 (1:1000 ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were incubated with 100 µl/well of diluted H3K9ac on a shaker at 4°C overnight and then treated with Alexa Fluor 488 conjugate secondary antibody (Cell Signaling #4412) at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: ... run using standard Ventana reagents a and Vector secondaries for staining with the TrkB.T1 SPEH1_D12 scFv-Fc fusion (Pattwell et al. 2020b) at 1:500 and PTEN (Cell Signaling #9188, Lot #4) at 1:100 or TrkB (kinase specific against amino acid 810 ...
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bioRxiv - Cell Biology 2022Quote: ... The membranes were incubated with 1:5 home-made anti-p53 (overnight at +4°C) and 1:100,000 anti-Lamin B1 (1 hr at room temperature) (Cell Signaling Technology, #12586). As secondary antibodies ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were then incubated overnight at 4 °C with primary antibodies diluted in blocking buffer: anti-myosin-5a antibody (1:100, #3402, Cell Signaling Technology), or anti-myosin-6 antibody (1:200 ...
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bioRxiv - Cancer Biology 2021Quote: ... followed by an overnight incubation at 4°C with the following primary antibodies: 4EBP1 (1:1,000, Cell Signaling Technology, Cambridge, UK #9644), MYCN (1:1,000 ...
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bioRxiv - Biochemistry 2020Quote: ... The membranes were then incubated overnight at 4 °C with either rabbit anti-p-MEK antibody against Ser217/Ser221 (1:1000) (Cell Signaling, #9121), rabbit anti-p-ERK antibody against Thr202/Tyr204 (1:1000 ...
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bioRxiv - Genomics 2020Quote: ... The samples were blocked in 10% goat serum and incubated overnight at 4°C with the mouse Cas9 antibody (7A9-3A3 Cell Signaling Technologies) 1:800 dilution in 1% BSA (Supp ...
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bioRxiv - Cell Biology 2020Quote: ... for 1 h at room temperature followed by incubation overnight at 4°C with a specific primary antibody to p-p53ser15 (Cell Signaling, 9284S), β-actin (Cell Signaling ...
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bioRxiv - Cell Biology 2020Quote: Cells were trypsinized and washed with PBS and then lysed for 30 minutes on a rotating wheel at +4 °C in RIPA buffer supplemented with protease/phosphatase inhibitor cocktail (Cell Signaling, 5872). Lysates were sonicated with a Bioruptor Soni cation System (UCD200 ...
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bioRxiv - Physiology 2021Quote: ... Blots were blocked in 5% bovine serum albumin in tris-buffered saline with 0.1% Tween 20 (TBS-T) and incubated overnight at 4°C with rabbit anti-phospo-Ser505-Akt (Cell Signaling Technology 4054) or mouse anti-α-Tubulin (DSHB 12G10 ...
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bioRxiv - Developmental Biology 2021Quote: ... incubated overnight at 4°C with a 1:1000 dilution of rabbit anti-phosphorylated-SMAD1/5/8 (clone 41D10, Cell Signaling Technologies) in block ...
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bioRxiv - Physiology 2021Quote: ... Sections were blocked with 5% donkey serum in the presence of 0.1% Triton followed by overnight incubation with primary antisera at 4 degrees (pCREB, Cell Signaling; Glucagon, Abcam). After extensive washing ...
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bioRxiv - Cancer Biology 2020Quote: ... The neutrophils were then incubated overnight at 4°C with primary antibodies: anti-histone H3 (1:50 dilution; Cell Signaling Technology, 3680) and either anti-NE (1:100 dilution ...
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bioRxiv - Immunology 2020Quote: ... The cell lysates were incubated overnight at 4 °C with NEMO antibody and ChIP-Grade Protein G Magnetic Beads (Cell Signaling Technology).
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bioRxiv - Microbiology 2021Quote: ... membranes were incubated at 4°C overnight with the following primary antibodies: rabbit anti-ubiquitin (1:1000; Cell Signaling Technology no. 3933), rabbit anti-phospho-eIF2α (1:1000 ...
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bioRxiv - Microbiology 2020Quote: ... incubated overnight at 4°C with the following primary antibodies diluted 1:1000 in 5% BSA/TBST: anti-pro-IL-1β (#12703, Cell Signaling Technology) and anti-NLRP3 (AG-20B-0014-C100 ...
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bioRxiv - Molecular Biology 2021Quote: ... The membranes were blocked with 5% BSA in TBST buffer for 2 h at RT and incubated overnight at 4°C with primary antibodies against ERK1/2 (1:1000; 9102s, Cell Signaling Technology), pERK1/2 (1:1000 ...
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bioRxiv - Immunology 2022Quote: ... Membranes were blocked for 1 hour at room temperature with blocking buffer (Licor) and incubated overnight at 4°C with anti-K48-linked poly-ubiquitin antibody (Cell Signaling Technologies) and anti-β-actin antibody (Santa Cruz Biotechnologies) ...
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bioRxiv - Developmental Biology 2022Quote: ... blots were incubated overnight at 4°C in the following primary antibodies: β-III-Tubulin (TUJ1, 1:1000; Cell Signaling Technology, 5568), SUFU (1:1000 ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then incubated with primary anti-RAB5 antibody at 4 °C overnight diluted in 8% BSA in PBST (1:500 dilution, Cat. #3547, lot 7, Cell Signaling Technology). Secondary anti-rabbit Alexa Fluor 568 antibody (1:1000 dilution ...
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bioRxiv - Biophysics 2022Quote: ... 15 μg of total protein was loaded onto an SDS-PAGE (4-12%) to assess LRP6 and β-catenin protein levels using phospho- and total LRP6 (Cell Signaling), active- and total β-catenin (Cell Signaling ...
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bioRxiv - Bioengineering 2022Quote: ... the membrane was incubated overnight at 4°C or with primary antibody solution (1:1000 dilution for anti-Alix [E4T7U, Cell Signaling Technology] ...