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• The Spread of Interferon-γ in Melanomas is Highly Spatially Confined, Driving Non-Genetic Variability in Tumor Cells

  Edoardo Centofanti, et al., bioRxiv - Systems Biology 2023

  Quote: ... Primary antibodies (anti-pSTAT1, clone 58D6, Cell Signaling Technologies) are applied at a dilution of 1:200 in blocking buffer in moist chambers for 1h at room temperature ...
• The Spread of Interferon-γ in Melanomas is Highly Spatially Confined, Driving Non-Genetic Variability in Tumor Cells

  Edoardo Centofanti, et al., bioRxiv - Systems Biology 2023

  Quote: ... B16-F10 cells were fixed as above and stained with anti-pSTAT1 (58D6: Cell Signaling Technologies). Live B16-F10 cells were stained with anti-MHC-I H2-Kb (AF6-88.5 ...
• Bromodomain-containing Protein 4 Regulates Innate Inflammation in Airway Epithelial Cells via Modulation of Alternative Splicing

  Morgan Mann, et al., bioRxiv - Systems Biology 2023

  Quote: ... 2 μg α-RelA antibody (Cell Signaling, Danvers, MA, USA, Cat. # 8242) was added to each extract ...
• Bromodomain-containing Protein 4 Regulates Innate Inflammation in Airway Epithelial Cells via Modulation of Alternative Splicing

  Morgan Mann, et al., bioRxiv - Systems Biology 2023

  Quote: ... Constant volumes of sample were incubated overnight with 2 μg α-BRD4 antibody (Cell Signaling, Danvers, MA, USA, Cat. # 13440) or a nonspecific isotype control (LSBio ...
• Comprehensive comparative assessment of the Arabidopsis thaliana MLO2-calmodulin interaction by various in vitro and in vivo protein-protein interaction assays

  Kira von Bongartz, et al., bioRxiv - Plant Biology 2023

  Quote: ... mouse α-His (Cell Signaling Technology; used in 1:1,000 dilution), rat α-HA (Hoffmann-La Roche AG ...
• Src-Family B Kinase Inhibition Promotes Lung Endothelial Dysfunction and Guides Drug Discovery

  Adam M. Andruska, et al., bioRxiv - Molecular Biology 2023

  Quote: ... phopho-P65 RelA (Cell Signaling Technologies 93H1,1:200). Appropriate secondary antibodies were used at a 1:250 dilution in a 5% serum PBS buffer for 1 hour ...
• Interferon-induced lysosomal membrane permeabilization and death cause cDC1-deserts in tumors

  E. Aerakis, et al., bioRxiv - Immunology 2023

  Quote: ... CD208/DC-LAMP rat monoclonal antibody (eurobio #Cat.DDX0192P-100) (1/100) and Cathepsin B rabbit antibody (Cell Signaling Cat.D1C7Y)(1/300 ...
• Exploring the Connection between Autophagy and Heat-Stress Tolerance in Drosophila melanogaster

  Quentin Willot, et al., bioRxiv - Physiology 2023

  Quote: ... Membranes were washed three times with TBS-T for 5 min and incubated with peroxidase-linked anti mouse or rabbit IgG at a 1:10,000 dilution in TBS-T (#7076/#7074S, Cell Signaling, Germany) for one hour at room temperature ...
• Profiling of the drug resistance of thousands of Src tyrosine kinase mutants uncovers a regulatory network that couples autoinhibition to catalytic domain dynamics

  Sujata Chakraborty, et al., bioRxiv - Biochemistry 2023

  Quote: ... All samples were separated via SDS–PAGE and visualized by western blotting with Src antibody (Cell Signaling, #2109 for WT and #2102 for SrcEEI) on Li-Cor Odyssey ...
• Tumor cell-adipocyte gap junctions activate lipolysis in breast cancer

  Roman Camarda, et al., bioRxiv - Cancer Biology 2023

  Quote: ... pHSL S563 (4139, Cell Signaling, 1:1000), HSL (4107 ...
• Distinct cellular origins and differentiation process account for distinct oncogenic and clinical behaviors of leiomyosarcomas

  Elodie Darbo, et al., bioRxiv - Cancer Biology 2023

  Quote: ... The anti-PTEN antibody (1:200, 9559, RRID:AB_390810, Clone 138G6, Cell Signaling Technology, Danvers, MA, USA) was diluted in Prep kit 26 (783-2876 ...
• Importance of Glutamine in Synaptic Vesicles Revealed by Functional Studies of SLC6A17 and Its Mutations Pathogenic for Intellectual Disability

  Xiaobo Jia, et al., bioRxiv - Neuroscience 2023

  Quote: ... The following antibodies were purchased from Cell Signaling Technology: rabbit anti-LAMP2 (cat.49067 for IB) ...
• Novel repertoire of tau biosensors to monitor pathological tau transformation and seeding activity in living cells

  Erika Cecon, et al., bioRxiv - Cell Biology 2023

  Quote: ... and immunoblotted with primary antibodies against the HA tag (rabbit, dilution 1:1000, cat. 3724S; Cell Signaling), or anti-phospho tau antibody AT8 (MN1020 ...
• Novel repertoire of tau biosensors to monitor pathological tau transformation and seeding activity in living cells

  Erika Cecon, et al., bioRxiv - Cell Biology 2023

  Quote: ... Tau-tau sensor molecules interaction was assessed using two monoclonal antibodies against the HA tag (mouse, Biolegend cat. 901514; rabbit, Cell Signaling 3724S), and two PLA probes (anti-rabbit-PLUS ...
• Novel repertoire of tau biosensors to monitor pathological tau transformation and seeding activity in living cells

  Erika Cecon, et al., bioRxiv - Cell Biology 2023

  Quote: ... Cells were then incubated (overnight, 4°C) with monoclonal anti-HA antibody (rabbit, dilution 1:500, cat. 3724S; Cell Signaling), or anti-tubulin (rat ...
• Tumor cell-adipocyte gap junctions activate lipolysis in breast cancer

  Roman Camarda, et al., bioRxiv - Cancer Biology 2023

  Quote: ... HSL (4107, Cell Signaling, 1:1000), HNF4α (ab41898 ...
• Human FAM111A inhibits vaccinia virus replication by degrading viral DNA-binding protein I3 and is antagonized by poxvirus host range factor SPI-1

  Junda Zhu, et al., bioRxiv - Microbiology 2023

  Quote: ... rabbit anti-LC3B antibody (#3868) and rabbit anti-ATG16L1 antibody (#8089) were purchased from Cell Signaling Technology; Goat anti-mouse IgG Alexa Fluor 488 (#A32723 ...
• Human FAM111A inhibits vaccinia virus replication by degrading viral DNA-binding protein I3 and is antagonized by poxvirus host range factor SPI-1

  Junda Zhu, et al., bioRxiv - Microbiology 2023

  Quote: ... The expression of ATG16L1 was determined by Western blotting using an anti-ATG16L1 antibody (Cell Signaling Technology).
• S-adenosylmethionine synthases specify distinct H3K4me3 populations and gene expression patterns during heat stress

  Adwait A. Godbole, et al., bioRxiv - Cell Biology 2023

  Quote: ... 1 uL anti-H3K4me3 antibody (Cell Signaling Technology, catalogue number C42D8) was added to the suspension and allowed to bind overnight at 4°C on a nutator shaker ...
• S-adenosylmethionine synthases specify distinct H3K4me3 populations and gene expression patterns during heat stress

  Adwait A. Godbole, et al., bioRxiv - Cell Biology 2023

  Quote: For H3K4me3 (Cell Signaling Technology, catalogue number C42D8) staining ...
• 5-HT_1A and 5-HT_2B receptor interaction and co-clustering regulates serotonergic neuron excitability

  Amina Benhadda, et al., bioRxiv - Neuroscience 2023

  Quote: ... Blots were probed with rabbit anti-HA (1:1,000; Cell Signaling#3724, RRID AB_1549585) or mouse anti-myc (1:1,000 ...
• 5-HT_1A and 5-HT_2B receptor interaction and co-clustering regulates serotonergic neuron excitability

  Amina Benhadda, et al., bioRxiv - Neuroscience 2023

  Quote: ... or the mouse anti-myc antibody (1:500; Cell Signaling #2276, RRID AB_331783) in PBS supplemented with Goat Serum (GS ...
• 5-HT_1A and 5-HT_2B receptor interaction and co-clustering regulates serotonergic neuron excitability

  Amina Benhadda, et al., bioRxiv - Neuroscience 2023

  Quote: ... Blots were probed with rabbit anti-HA (1:1,000; Cell Signaling#3724, RRID AB_1549585) or mouse anti-myc (1:1,000 ...
• 5-HT_1A and 5-HT_2B receptor interaction and co-clustering regulates serotonergic neuron excitability

  Amina Benhadda, et al., bioRxiv - Neuroscience 2023

  Quote: ... Respective expression was assessed by immunostaining using a mouse anti-HA antibody (1:500; Cell Signaling#2367, RRID AB_10691311 and a rabbit anti-Flag (1:500; Cell Signaling#14793 RRID AB_2572291 followed by Cy5-conjugated donkey anti-mouse (1.9 µg/ml ...
• 5-HT_1A and 5-HT_2B receptor interaction and co-clustering regulates serotonergic neuron excitability

  Amina Benhadda, et al., bioRxiv - Neuroscience 2023

  Quote: ... or mouse anti-myc (1:1,000; Cell Signaling#2276, RRID AB_331783). Secondary antibodies (1:10,000 ...
• 5-HT_1A and 5-HT_2B receptor interaction and co-clustering regulates serotonergic neuron excitability

  Amina Benhadda, et al., bioRxiv - Neuroscience 2023

  Quote: ... Neurons were then incubated for 1 h with either the rabbit anti-HA antibody (1:500; Cell Signaling #3724, RRID AB_1549585) or the mouse anti-myc antibody (1:500 ...
• 5-HT_1A and 5-HT_2B receptor interaction and co-clustering regulates serotonergic neuron excitability

  Amina Benhadda, et al., bioRxiv - Neuroscience 2023

  Quote: ... Lysates were co-imunoprecipitated with anti-HA beads (HA-Tag C29F4 Rabbit mAb Sepharose® Bead Conjugate Cell Signaling #3956 RRID:AB_10695091) overnight at 4 °C under gentle agitation ...
• Phosphorylation of pyruvate dehydrogenase marks the inhibition of in vivo neuronal activity

  Dong Yang, et al., bioRxiv - Neuroscience 2023

  Quote: ... rabbit monoclonal anti-pPDH (Ser293) (1:1000; Cell Signaling #37115) in Figure S2F ...
• Phosphorylation of pyruvate dehydrogenase marks the inhibition of in vivo neuronal activity

  Dong Yang, et al., bioRxiv - Neuroscience 2023

  Quote: ... and then incubated with Alexa Fluor 647 direct-conjugated rabbit monoclonal anti-pPDH (Ser293) (#37115, custom conjugated by Cell Signaling).
• Phosphorylation of pyruvate dehydrogenase marks the inhibition of in vivo neuronal activity

  Dong Yang, et al., bioRxiv - Neuroscience 2023

  Quote: ... slices were stained with rabbit monoclonal anti-pPDH (Ser293, Cell Signaling #37115) and mouse monoclonal anti-cFos (Santa Cruz Biotechnology #sc-271243) ...
• Phosphorylation of pyruvate dehydrogenase marks the inhibition of in vivo neuronal activity

  Dong Yang, et al., bioRxiv - Neuroscience 2023

  Quote: ... rabbit anti-Orexin (1:100, Cell Signaling #16743), rabbit anti-MCH (1:200 ...
• Phosphorylation of pyruvate dehydrogenase marks the inhibition of in vivo neuronal activity

  Dong Yang, et al., bioRxiv - Neuroscience 2023

  Quote: The primary antibodies used include: rabbit monoclonal anti-pPDH (Ser293) (1:500, Cell Signaling #37115), mouse monoclonal anti-cFos (1:500 ...
• A Consequence of Immature Breathing induces Persistent Changes in Hippocampal Synaptic Plasticity and Behavior: A Role of Pro-Oxidant State and NMDA Receptor Imbalance

  Alejandra Arias-Cavieres, Alfredo J. Garcia III, bioRxiv - Neuroscience 2023

  Quote: ... anti-rabbit GluN2B (1:2000; Cell Signaling Technology Cat# 14544, RRID:AB_2798506), anti-rabbit NOX2 (1:2000 ...
• A Consequence of Immature Breathing induces Persistent Changes in Hippocampal Synaptic Plasticity and Behavior: A Role of Pro-Oxidant State and NMDA Receptor Imbalance

  Alejandra Arias-Cavieres, Alfredo J. Garcia III, bioRxiv - Neuroscience 2023

  Quote: ... anti-rabbit GluN2A (1:2000; Cell Signaling Technology Cat# 4205, RRID:AB_2112295), anti-rabbit GluN2B (1:2000 ...
• Automated Enrichment of Phosphotyrosine Peptides for High-Throughput Proteomics

  Alexis Chang, et al., bioRxiv - Systems Biology 2023

  Quote: ... p-Tyr-100 and p-Tyr-1000 antibodies were purchased as magnetic bead conjugates from Cell Signaling Technology® and mixed in a 1:1 ratio by volume ...
• Effects of Dopamine Receptor Antagonists and Radiation on Mouse Neural Stem/Progenitor Cells

  Ling He, et al., bioRxiv - Neuroscience 2023

  Quote: ... Slides were mounted with ProLong® Gold Antifade Reagent with DAPI (Cell signaling, Cat #8961S). Images were captured and merged at 20X using a digital microscope (BZ-9000 ...
• PIC recruitment by synthetic reader-actuators to polycomb-silenced genes blocks triple-negative breast cancer invasion

  Natecia L. Williams, et al., bioRxiv - Synthetic Biology 2023

  Quote: ... rabbit anti-GAPDH 1:1000 (Cell Signaling Technology, 2118S). Secondary staining was performed with 1:30000 goat anti-rabbit IRDye 800CW-conjugated (LI-COR BioScience ...
• PIC recruitment by synthetic reader-actuators to polycomb-silenced genes blocks triple-negative breast cancer invasion

  Natecia L. Williams, et al., bioRxiv - Synthetic Biology 2023

  Quote: ... rabbit anti-VIN 1:1000 (Cell Signaling Technology, 13901S), rabbit anti-GAPDH 1:1000 (Cell Signaling Technology ...
• Phosphoproteomics of cellular mechanosensing reveals NFATC4 as a regulator of myofibroblast activity

  Laura F. Mattner, et al., bioRxiv - Systems Biology 2023

  Quote: ... Anti-Phospho-Myosin Light Chain 2 (Thr18/Ser19) (1:2000, Cell Signaling #3674), Anti-Phospho-FoxO3a (S7 ...
• Phosphoproteomics of cellular mechanosensing reveals NFATC4 as a regulator of myofibroblast activity

  Laura F. Mattner, et al., bioRxiv - Systems Biology 2023

  Quote: ... Anti-Phospho-CBX3 (Ser93) (1:1000, Cell Signaling #2600), Anti-rabbit IgG ...
• Neutralizing Antibodies with Neurotropic Factor Treatment Maintain Neurodevelopmental Gene Expression Upon Exposure to Human Cytomegalovirus

  Benjamin S. O’Brien, et al., bioRxiv - Neuroscience 2023

  Quote: ... blots were incubated in blocking buffer for 1 hour and probed using primaries Lamin B/C (Cell Signaling, 4777S), STAT3 (Cell Signaling ...
• A role for Toll-like receptor 3 in lung vascular remodeling associated with SARS-CoV-2 infection

  Daniela Farkas, et al., bioRxiv - Molecular Biology 2023

  Quote: ... TLR3 (6961, Cell Signaling Technology, Danvers, MA).
• Targeted cancer therapy induces APOBEC fuelling the evolution of drug resistance

  Manasi K. Mayekar, et al., bioRxiv - Cancer Biology 2023

  Quote: ... anti-TUBB (#2146) and anti-histone H3 (#9715) were purchased from Cell Signaling Technology.
• Targeted cancer therapy induces APOBEC fuelling the evolution of drug resistance

  Manasi K. Mayekar, et al., bioRxiv - Cancer Biology 2023

  Quote: ... IHC was performed using the following primary antibodies: EGFRL858R mutant specific (Cell Signaling: 3197, 43B2), APOBEC3B (5210-87-13)57 ...
• Extravascular spaces are the primary reservoir of antigenic diversity in Trypanosoma brucei infection

  Alexander K. Beaver, et al., bioRxiv - Microbiology 2023

  Quote: ... secondary antibody staining was performed for 10 minutes at 4°C with Anti-Rabbit IgG conjugated to Alexa Fluor® 488 fluorescent dye (Cell Signaling Technology, 4412S). Finally ...
• Short-term molecular consequences of chromosome mis-segregation for genome stability

  Lorenza Garribba, et al., bioRxiv - Cell Biology 2023

  Quote: hTERT RPE-1 Cdc7 wt or as cells or hTERT RPE-1 cells treated as above were harvested and lysed using RIPA buffer (1x, Cell Signaling Technology) supplemented with protease inhibitor cocktail (Roche ...
• Short-term molecular consequences of chromosome mis-segregation for genome stability

  Lorenza Garribba, et al., bioRxiv - Cell Biology 2023

  Quote: ... GAPDH (1:2000, #2118, Cell Signaling Technology) and vinculin (1:5000 ...
• Short-term molecular consequences of chromosome mis-segregation for genome stability

  Lorenza Garribba, et al., bioRxiv - Cell Biology 2023

  Quote: ... anti-pChk1 (#133D3, Cell Signaling Technology) 1:50 ...
• Short-term molecular consequences of chromosome mis-segregation for genome stability

  Lorenza Garribba, et al., bioRxiv - Cell Biology 2023

  Quote: ... cells were stained using the Senescence β-Galactosidase Staining Kit (Cell Signaling Technology) following manufacturer’s instructions and images were acquired using the EVOS imaging system (Thermo Fisher Scientific).
• Targeting the Cx26/NANOG/Focal Adhesion Kinase Complex via Cell-Penetrating Peptides in Triple-Negative Breast Cancers

  Erin E. Mulkearns-Hubert, et al., bioRxiv - Cancer Biology 2023

  Quote: ... Membranes were blocked in 5% non-fat milk in TBST for 1 h and incubated with the appropriate primary antibody overnight at 4°C: anti-NANOG (D73G4) (Cell Signaling #4903; 1:1000), anti-FAK (D2R2E ...