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• PRDM3/16 Regulate Chromatin Accessibility Required for NKX2-1 Mediated Alveolar Epithelial Differentiation and Function

  Hua He, et al., bioRxiv - Developmental Biology 2023

  Quote: ... Blots were incubated with Rabbit mAb anti-DYKDDDK Tag (HRP Conjugated) (Cell Signaling Technology, 86861, 1:1000, RRID:AB_2800094) or NKX2-1 antibody (Seven Hills Bioreagents ...
• Interneuron migration defects during corticogenesis contribute to Dyrk1a haploinsufficiency syndrome pathogenesis via actomyosin dynamics deregulations

  Maria Victoria Hinckelmann, et al., bioRxiv - Developmental Biology 2023

  Quote: ... rabbit anti-myosin light chain 2 (Cell signaling, 1:1000), rabbit anti-phospho myosin light chain 2 (Cell signaling ...
• Control of mitophagy initiation and progression by the TBK1 adaptors NAP1 and SINTBAD

  Elias Adriaenssens, et al., bioRxiv - Cell Biology 2023

  Quote: ... or (1:1000, Cell Signaling Technology Cat# 31219, RRID:AB_2936222), anti-FIP200 (1:1000 ...
• Control of mitophagy initiation and progression by the TBK1 adaptors NAP1 and SINTBAD

  Elias Adriaenssens, et al., bioRxiv - Cell Biology 2023

  Quote: ... or (1:1000, Cell Signaling Technology Cat# 3724, RRID:AB_1549585), anti-mHSP60 (1:1000 ...
• Control of mitophagy initiation and progression by the TBK1 adaptors NAP1 and SINTBAD

  Elias Adriaenssens, et al., bioRxiv - Cell Biology 2023

  Quote: ... anti-ATG5 (1:1000, Cell Signaling Technology Cat# 12994, RRID:AB_2630393), anti-β-Actin (1:5000 ...
• Control of mitophagy initiation and progression by the TBK1 adaptors NAP1 and SINTBAD

  Elias Adriaenssens, et al., bioRxiv - Cell Biology 2023

  Quote: ... or (1:1000, Cell Signaling Technology Cat# 3013, RRID:AB_2199749), anti-phospho-TBK1 S172 (1:1000 ...
• The RNA binding protein, HNRNPA2B1, regulates IFNG signaling in macrophages

  Mays Mohammed Salih, et al., bioRxiv - Immunology 2023

  Quote: ... STAT3 (1:500, Cell Signaling, 9139S), pJAK3 (1:500 ...
• The RNA binding protein, HNRNPA2B1, regulates IFNG signaling in macrophages

  Mays Mohammed Salih, et al., bioRxiv - Immunology 2023

  Quote: ... pSTAT3 (1:500, Cell Signaling, 9138S), STAT3 (1:500 ...
• The RNA binding protein, HNRNPA2B1, regulates IFNG signaling in macrophages

  Mays Mohammed Salih, et al., bioRxiv - Immunology 2023

  Quote: ... pJAK3 (1:500, Cell Signaling, 5031S), or JAK3 (1:500 ...
• The RNA binding protein, HNRNPA2B1, regulates IFNG signaling in macrophages

  Mays Mohammed Salih, et al., bioRxiv - Immunology 2023

  Quote: ... or JAK3 (1:500, Cell Signaling, 8863S). Horseradish peroxidase-conjugated β-actin (1:500 ...
• Galectin-9 regulates dendritic cell contractility and migration via RhoA

  Guus Franken, et al., bioRxiv - Immunology 2023

  Quote: ... rabbit anti-phospho(Thr 423) Pak1 (#2601; Cell Signaling Technology) at 1:500 (v/v) ...
• Galectin-9 regulates dendritic cell contractility and migration via RhoA

  Guus Franken, et al., bioRxiv - Immunology 2023

  Quote: ... rabbit anti-GAPDH (#2118; Cell Signaling Technology) at 1:500 (v/v) ...
• The interaction between NLRP1 and oxidized TRX1 involves a transient disulfide bond

  Michael B. Geeson, et al., bioRxiv - Immunology 2023

  Quote: ... HA mouse monoclonal Ab (Cell Signaling Tech, 6E2), FLAG mouse monoclonal Ab (Sigma Aldrich ...
• The interaction between NLRP1 and oxidized TRX1 involves a transient disulfide bond

  Michael B. Geeson, et al., bioRxiv - Immunology 2023

  Quote: Antibodies used include: Thioredoxin 1 (ab86255, Abcam; 2429S, Cell Signaling Technology), DPP9 (ab42080 ...
• Nuclear corepressors NCOR1 and NCOR2 entrain thymocyte signaling, selection, and emigration

  Natalie A. David, et al., bioRxiv - Immunology 2023

  Quote: ... NCOR1 (Cell Signaling Technology 5948; 1:100), donkey anti-rabbit IgG (eBioscience 12-4739-81 ...
• Dectin-1 ligands produce distinct training phenotypes in human monocytes through differential activation of signaling networks

  Quen J. Cheng, et al., bioRxiv - Immunology 2023

  Quote: ... p-STAT1 (Rabbit IgG, 1:1000, Cell Signaling Technology 9167), and p84 (Rabbit IgG ...
• Dectin-1 ligands produce distinct training phenotypes in human monocytes through differential activation of signaling networks

  Quen J. Cheng, et al., bioRxiv - Immunology 2023

  Quote: ... The following antibodies were used for immunoblotting: p-cJUN (Rabbit IgG, 1:2000, Cell Signaling Technology 9164), pIRF-3 (Rabbit IgG ...
• Dectin-1 ligands produce distinct training phenotypes in human monocytes through differential activation of signaling networks

  Quen J. Cheng, et al., bioRxiv - Immunology 2023

  Quote: ... and an:-rabbit IgG (Goat IgG-HRP, 1:5000, Cell Signaling Technology 7074). Immunoblots were developed using chemiluminescent substrate (Supersignal West Pico Plus ...
• Replication stress underlies genomic instability at CTCF/cohesin-binding sites in cancer

  Elangoli Ebrahimkutty Faseela, et al., bioRxiv - Genomics 2023

  Quote: ... Antibodies used are: CTCF (D31H2: Cell Signaling Technology, 1µg), RAD21 (ab992 ...
• Host-derived oxidized phospholipids initiate effector-triggered immunity fostering lethality upon microbial encounter

  Marco Di Gioia, et al., bioRxiv - Immunology 2023

  Quote: ... phospho-AKT (Thr308) (1:1000, Cell Signaling Technology, Cat# 13038), AKT (pan ...
• Host-derived oxidized phospholipids initiate effector-triggered immunity fostering lethality upon microbial encounter

  Marco Di Gioia, et al., bioRxiv - Immunology 2023

  Quote: ... TBK1 (1:1000, Cell Signaling Technology, Cat# 38066), phospho-IKKε (Ser172 ...
• Host-derived oxidized phospholipids initiate effector-triggered immunity fostering lethality upon microbial encounter

  Marco Di Gioia, et al., bioRxiv - Immunology 2023

  Quote: ... phospho-NDRG1 (Thr346) (1:1000, Cell Signaling Technology, Cat# 5482), NDRG1 (1:1000 ...
• Host-derived oxidized phospholipids initiate effector-triggered immunity fostering lethality upon microbial encounter

  Marco Di Gioia, et al., bioRxiv - Immunology 2023

  Quote: ... RICTOR (1:1000, Cell Signaling Technology, Cat# 2114), phospho-PKCα/βII (Thr638/641 ...
• Host-derived oxidized phospholipids initiate effector-triggered immunity fostering lethality upon microbial encounter

  Marco Di Gioia, et al., bioRxiv - Immunology 2023

  Quote: ... p70S6K (1:1000, Cell Signaling Technology, Cat# 34475), phospho-S6 (Ser235/Ser236 ...
• Host-derived oxidized phospholipids initiate effector-triggered immunity fostering lethality upon microbial encounter

  Marco Di Gioia, et al., bioRxiv - Immunology 2023

  Quote: ... IKKε (1:1000, Cell Signaling Technology, Cat# 3416), phospho-PDK1 (Ser241 ...
• Host-derived oxidized phospholipids initiate effector-triggered immunity fostering lethality upon microbial encounter

  Marco Di Gioia, et al., bioRxiv - Immunology 2023

  Quote: ... phospho-RICTOR (Thr1135) (1:1000, Cell Signaling Technology, Cat# 3806), RICTOR (1:1000 ...
• A blood-brain-barrier penetrant AAV gene therapy rescues neurological deficits in mucolipidosis IV mice

  Madison Sangster, et al., bioRxiv - Genetics 2023

  Quote: ... and GFAP (Mouse, 1:1000; Cell Signaling Technology, Danvers, MA, Cat. ID: 3670S) were diluted in 5% NGS and 2% BSA and applied overnight at 4°C ...
• The Chordate Origins of Heart Regeneration

  Keaton J. Schuster, Lionel Christiaen, bioRxiv - Developmental Biology 2023

  Quote: ... rabbit anti-pSmad1/5/8 41D10 (Cell Signaling Technology) 1:250 ...
• High-resolution CTCF footprinting reveals impact of chromatin state on cohesin extrusion dynamics

  Corriene E. Sept, et al., bioRxiv - Genomics 2023

  Quote: ... and CTCF ChIP was performed using 1500ng of chromatin (40-70% mononucleosomes) and 500 ng of CTCF antibody (Cell Signaling, cat #: 3418). Protein A/G beads pull-down ...
• Loss of G9a does not phenocopy the requirement for Prdm12 in the development of the nociceptive neuron lineage

  Panagiotis Tsimpos, et al., bioRxiv - Developmental Biology 2023

  Quote: ... and Normal Rabbit IgG (Cell Signaling, 2729). Secondary antibodies used in this study were Goat α-mouse IgG HRP (Jackson ImmunoResearch ...
• Cdc73 majorly regulates apoptosis-inducing factor (AIF1) in Saccharomyces cerevisiae via the H3K36 methylation

  Nitu Saha, et al., bioRxiv - Genetics 2023

  Quote: ... α-H3K4Me3 (Cell Signaling), α-H3K36Me3 and goat anti-rabbit IgG secondary antibody (Invitrogen ...
• Characterization of the Pristionchus pacificus “epigenetic toolkit” reveals the evolutionary loss of the histone methyltransferase complex PRC2

  Audrey Brown, et al., bioRxiv - Genetics 2023

  Quote: ... Primary antibodies used were Rabbit Tri-Methyl-Histone H3 (C36B11) (Cell Signaling, #9733) and Histone H3 (1B1B2 ...
• The balance between ATR and DDK activities controls TopBP1-mediated locking of dormant origins at the pre-IC stage

  Stéphane Koundrioukoff, et al., bioRxiv - Genetics 2023

  Quote: Rabbit anti-CHK1 S317Ph (12302, Cell Signaling Technology)
• Extramacrochaetae regulates Notch signaling in the Drosophila eye through non-apoptotic caspase activity

  Sudershana Nair, Nicholas E. Baker, bioRxiv - Developmental Biology 2023

  Quote: ... Other antibodies: anti-phospho-Smad1/5 (rabbit, 1:100, Cell Signaling), anti- DIAP1 (mouse ...
• CEBPA restricts alveolar type 2 cell plasticity during development and injury-repair

  Dalia Hassan, Jichao Chen, bioRxiv - Developmental Biology 2023

  Quote: ... the following antibodies were used: rabbit anti-CCAAT/enhancer binding protein alpha (C/EBPA, 1:500, 8178P, Cell Signaling Technology), chicken anti-green fluorescent protein (GFP ...
• The DNA helicase FANCJ (BRIP1) functions in Double Strand Break repair processing, but not crossover formation during Prophase I of meiosis in male mice

  Tegan S. Horan, et al., bioRxiv - Genetics 2023

  Quote: ... HA-Tag (6E2) (mouse) mAb 1:100 (Cell Signaling Technology 2367); SYCP3 (mouse ...
• Rtf1-dependent transcriptional pausing regulates cardiogenesis

  Adam D. Langenbacher, et al., bioRxiv - Developmental Biology 2023

  Quote: ... Immunoprecipitations were carried out as previously described63 on approximately 5 ug of chromatin (minimum 2.5 ug, maximum 8.8 ug) using 5 ul of total RNA Pol II antibody (Cell Signaling D8L4Y). Libraries were prepared using the NEBNext Ultra II DNA Library Kit for Illumina (NEB ...
• Actin-based deformations of the nucleus control multiciliated ependymal cell differentiation

  Marianne Basso, et al., bioRxiv - Developmental Biology 2023

  Quote: ... Lapin anti-phospho-RB (Cell Signaling 8516), rabbit anti-Nesprin2 (1/100 ...
• The DNA helicase FANCJ (BRIP1) functions in Double Strand Break repair processing, but not crossover formation during Prophase I of meiosis in male mice

  Tegan S. Horan, et al., bioRxiv - Genetics 2023

  Quote: ... HA-Tag (C29F4) (rabbit) mAb 1:100 (Cell Signaling Technology 3724); HA-Tag (6E2 ...
• The DNA helicase FANCJ (BRIP1) functions in Double Strand Break repair processing, but not crossover formation during Prophase I of meiosis in male mice

  Tegan S. Horan, et al., bioRxiv - Genetics 2023

  Quote: ... HA-Tag (6E2) (mouse) mAb 1:1000 (Cell Signaling Technology 2367); TOPBP1 (rabbit ...
• Disruption of IL-17 signaling in the respiratory mucosa results in invasive streptococcal infection

  Jamie-Lee Mills, et al., bioRxiv - Immunology 2023

  Quote: ... IHC staining of spleen sections for CD4 (1:200, D7D2Z; Cell Signaling Technology) and neutrophil elastase (1:200 ...
• E-proteins set the threshold for optimal TCF1 expression during αβ T cell development

  Anjali Verma, et al., bioRxiv - Immunology 2023

  Quote: ... The resulting fragments were immunoprecipitated with 5 μg of anti-Notch antibody (Cell Signaling Technology #3608) with Protein G Dynabeads (Invitrogen 10003D) ...
• Following the p63/Keratin5 Basal Cells in the Sensory and Non-sensory Epithelia of The Vomeronasal Organ

  Noah M. LeFever, et al., bioRxiv - Developmental Biology 2023

  Quote: ... *Rabbit anti-Ki67 (1:1000, D3B5, Cell signaling Tech), *Mouse anti-Ki67 (1:500 ...
• Following the p63/Keratin5 Basal Cells in the Sensory and Non-sensory Epithelia of The Vomeronasal Organ

  Noah M. LeFever, et al., bioRxiv - Developmental Biology 2023

  Quote: ... *Mouse anti-Ki67 (1:500, 9449, Cell signaling Tech), *Mouse anti-DsRd (1:500 ...
• The L27 Domain of MPP7 enhances TAZ-YY1 Cooperation to Renew Muscle Stem Cells

  Anwen Shao, Joseph L. Kissil, Chen-Ming Fan, bioRxiv - Developmental Biology 2023

  Quote: ... or rabbit anti-FLAG antibodies (Cell Signaling), followed by HRP-conjugated goat anti-rabbit antibodies (Bio-Rad) ...
• The conserved wobble uridine tRNA thiolase Ctu1 is required to sustain development and differentiation

  YZW Yu, CQ Wang, Y Wang, H Shi, ZL Zhou, bioRxiv - Developmental Biology 2023

  Quote: ... Quantitative PCR (qPCR) analysis was performed on a QuantStudio™ 5 Real-Time PCR System (Cell Signaling Technology, MA) using the SYBR green reaction mix (Vazyme Biotech ...
• Hallmark molecular and pathological features of POLG disease are recapitulated in cerebral organoids

  Anbin Chen, et al., bioRxiv - Cell Biology 2023

  Quote: ... NeuN (rabbit, 1:500; Cell Signaling, #24307S), MAP2 (rabbit ...
• PRDM3/16 Regulate Chromatin Accessibility Required for NKX2-1 Mediated Alveolar Epithelial Differentiation and Function

  Hua He, et al., bioRxiv - Developmental Biology 2023

  Quote: ... and Multiplex-oligos for Illumina Systems (Cell Signaling Technology, 47538S). Libraries were sequenced by Genewiz on a Hiseq4000 with PE150 mode for at least 15 million reads per sample.
• Extramacrochaetae regulates Notch signaling in the Drosophila eye through non-apoptotic caspase activity

  Sudershana Nair, Nicholas E. Baker, bioRxiv - Developmental Biology 2023

  Quote: ... anti-Dcp1 (rabbit, 1:100, Cell Signaling), anti-Sens (guinea pig ...
• Dynamic Hippo pathway activity underlies mesenchymal differentiation during lung alveolar morphogenesis

  Fatima N. Chaudhry, et al., bioRxiv - Developmental Biology 2023

  Quote: ... rabbit anti-YAP (1:100 with amplification, cat. # 4912, Cell Signaling), guinea pig anti-RFP (1:100 ...