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Citations for Cell Signaling Technology :
5201 - 5250 of 10000+ citations for Mouse Anti Bovine Coronavirus Spike Antibody 5A4 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... The permeabilized cells then were incubated with an anti-HA AlexaFluor®488 conjugated primary antibody (1:800) from Cell Signaling (ID: 2350S). The cells were also stained with DAPI to detect DNA and then were viewed using an Evos FL Cell Imaging System from Thermofisher (100 x objective) ...
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bioRxiv - Cell Biology 2021Quote: ... Sonicated chromatin was diluted and added to 0.2 ml PCR tube containing primary antibodies (3ug of rabbit anti-NKX6.1 cat. 54551 (Cell Signaling Technology, Danvers, MA, USA); 3 μg of rabbit-anti-acetyl-histone H3-cat ...
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bioRxiv - Genomics 2022Quote: Antibodies used for CUT&Tag or MulTI-Tag in this study were as follows: Rabbit Anti-H3K27me3 (Cell Signaling Technologies CST9733S, Lot 16), Mouse anti-RNA PolIIS5P (Abcam ab5408 ...
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bioRxiv - Cancer Biology 2022Quote: ... The following antibodies were used for cleavage under targets & release using nuclease (CUT&RUN) assays: anti-H3K27ac (8173, Cell Signaling Technology, 1:100) and anti-H3K4me1 (5326 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Immunoblotting analysis was performed with the following monoclonal primary antibodies: rabbit anti p-mTOR (1:1000; #5536, Cell Signaling Technology, Danvers, MA, USA) and rabbit anti mTOR (1:1000 ...
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bioRxiv - Microbiology 2022Quote: ... The fluorescent-labelled Alexa Fluor 647 (AF647) goat anti-Rabbit IgG monoclonal antibody was from Cell Signaling Technologies (MA, USA; Cat. n° 4414).
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bioRxiv - Immunology 2022Quote: ... the membrane was incubated for one hour at RT with peroxidase-conjugated goat anti-rabbit secondary antibody (Cell Signaling Technology, #7074, 1:1000) and washed again ...
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bioRxiv - Neuroscience 2020Quote: ... Slices were then incubated for 48h at 4°C with the following primary antibodies: rabbit anti-phospho-S6 (1:1000, Cell Signaling, Cat# 5364), mouse anti-NeuN (1:400 ...
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bioRxiv - Neuroscience 2021Quote: Primary antibodies for western blots were diluted as follows: rabbit anti-Phospho-AKT Ser473 (D9E) (Cell Signaling Technology, USA; RRID: AB_2797780; 1:2000), mouse anti-Pan-AKT (40D4 ...
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bioRxiv - Cancer Biology 2020Quote: ... 50 μL of empty Dynabeads® Protein A was washed by Citrate-Phosphate Buffer (pH 5.0) and then incubated with 15ul Rabbit Monoclonal anti-UPF1 antibody (Cell Signaling Technology, Cat# 12040) or 5ug Normal Rabbit IgG Polyclonal Antibody (Merck ...
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bioRxiv - Cancer Biology 2020Quote: ... 50 μL of empty Dynabeads® Protein A were washed by Citrate-Phosphate Buffer (pH 5.0) and then incubated with 15ul Rabbit Monoclonal anti-UPF1 antibody (Cell Signaling TECHNOLOGY, Cat# 12040) or 5ug Rabbit polyclonal anti-phospho-Upf1 (Ser1127 ...
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bioRxiv - Neuroscience 2020Quote: ... Biotinylated proteins were boiled with SDS loading buffer and then resolved with SDS-PAGE and subjected to Western blot analysis using anti-MeCP2 antibody (Cell Signaling Technology, 3456). To quantify the level of O-GlcNAcylation ...
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bioRxiv - Neuroscience 2020Quote: ... Western Blot was performed using an anti-Scn2a antibody (Alomone, Jerusalem, Israel) and a β-actin control (Cell Signaling Technology, Danvers, MA, USA).
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bioRxiv - Cancer Biology 2020Quote: ... for 2 hours and blotted at 4°C overnight with primary antibodies as follows: rabbit anti-phospho-GSK-3β (Ser9) (5B3) (1/500 dilution; Cell Signaling; cat #9323), rabbit anti-GSK-3β (1/1000 dilution ...
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bioRxiv - Immunology 2022Quote: ... cells were blocked for 1h with 5% BSA in PBS and then stained with rabbit anti-pPLCγ1 antibody (Cell Signaling, #2821, 1/100) overnight at 4C ...
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bioRxiv - Neuroscience 2019Quote: ... Primary antibodies against pSTAT3 and pAKT were detected by using a conformation-specific HRP-conjugated anti-rabbit IgG secondary antibody (1:4,000; Cell Signaling Technologies; RRID: AB_10892860). Antigen-antibody complexes were visualized by using enhanced chemiluminescence substrate (Bio-Rad ...
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bioRxiv - Biochemistry 2021Quote: ... boiled in sample buffer for 5 min and subjected to immunoblot assay with anti-HA antibody (Cell Signaling Technology, Inc., #3724, Danvers, MA).
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bioRxiv - Cell Biology 2020Quote: ... of embedded organoids or whole-mounted organoids were incubated overnight at 4°C with rabbit monoclonal anti-p63a antibody (D2K8X, Cell Signaling Technology, MA), polyclonal guinea pig anti-cytokeratin 8/18 (03-GP11 ...
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bioRxiv - Microbiology 2020Quote: ... Arf6 excision in endothelial cells was verified by isolating lung endothelial cells as previously described (28) and performing a Western blot to assess ARF6 levels using anti-ARF6 antibody from Cell Signaling Technology (#3546) (S5 Figure B).
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bioRxiv - Genetics 2021Quote: ... Membranes were rinsed with TBST for 30 min before incubation with horseradish peroxidase-conjugated anti-rabbit secondary antibody (Cell Signaling; 7074S, 1:5000) for 1 h ...
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bioRxiv - Cell Biology 2020Quote: ... Co-IP was carried out in the same way above using NE-4C and HepG2 cells and an anti-PRMT1/Prmt1 antibody (Cell Signaling Technology, #2449). Precipitated proteins were subjected to SDS-PAGE ...
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bioRxiv - Biochemistry 2020Quote: ... The membrane was washed 1× TBST three times for 10 min each followed by incubation with anti-rabbit IgG horseradish-peroxidase secondary antibody conjugate (1:3000 dilution; Cell Signaling Technology, 7074S) in 1× TBS for 2 h ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein bound beads were boiled with 1X SDS loading buffer and resolved in 10% SDS-PAGE and analyzed by with 1:1000 dilution of rabbit anti-PAX9 polyclonal antibody (Cell Signaling Technology, USA) or 1:1000 dilution of mouse anti-FLAGM2 monoclonal antibody (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2021Quote: Co-immunoprecipitation of endogenous PARP1 and SET8 were performed with 200 µg of total extract from crosslinked (1% formaldehyde for 10 min) HCT116 or HeLa cells using anti-PARP1 antibody (Cell Signaling Technology # 9532), anti-SET8 antibody (Santa Cruz Biotechnology # sc-515433 ...
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The Kinase Chemogenomic Set (KCGS): An open science resource for kinase vulnerability identificationbioRxiv - Cell Biology 2019Quote: ... and a 1:1000 dilution of anti-phospho-histone H3 (pHH3) Alexa 488 (Ser10, clone D2C8) conjugated antibody (Cell Signaling Technologies, Danvers, MA). Fixed cells were imaged with a 10x objective using an IXM-C microscope and analyzed using MetaXpress software (Molecular Devices ...
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bioRxiv - Physiology 2021Quote: ... anti-phospho and total extracellular signal-regulated kinase antibody (p/total ERK 1-2 1:1000 CST4396, CST4696, Cell Signaling Technology, Danvers, MA), anti-phospho and total protein kinase B antibody (AKT 1:1000 ...
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bioRxiv - Cell Biology 2021Quote: ... through an Azure c600 gel imaging system and bands were quantified with an Azurespot software (Dublin, CA, USA). Anti-SEC24A antibody (Cat. 9678, RRID:AB_10949103) was purchased from Cell Signaling Technology (Danvers, MA, USA). Anti-fibronectin 1 antibody (15613-1-AP ...
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bioRxiv - Molecular Biology 2021Quote: ... 9ug/ml) obtained from LS Bio (Seattle,WA) followed by horseradish peroxidase linked anti-rabbit secondary antibody (Cell Signaling Technology Inc., Beverly, MA). Slides were developed with DAB substrate ...
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bioRxiv - Cell Biology 2021Quote: ... rabbit monoclonal anti-SorLA antibody specific to the N-terminus of human SorLA protein (1:1,000; D8D4G #79322; Cell Signaling, Danvers, MA, USA) for the detection of SorLA759 proteins ...
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bioRxiv - Developmental Biology 2021Quote: ... Blocked membranes were incubated overnight at 4°C with primary antibodies diluted in blocking solution: rabbit anti-Ezh2 (Cell Signaling Technologies; 1:1000), rabbit anti-H3K27me3 (Millipore ...
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bioRxiv - Cancer Biology 2022Quote: ... The slides were incubated overnight with the following primary antibodies at the indicated dilutions: rabbit monoclonal anti-HA 1:1000 (Cell Signaling Technology 3724), rabbit polyclonal anti-PTEN 1:400 (Thermo Fisher 51-2400) ...
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bioRxiv - Microbiology 2022Quote: ... The membrane was washed 4x with PBST for 5 minutes each and incubated for 1 hour in the dark at room temperature with anti-rabbit IgG HRP-linked secondary antibody (Cell Signaling, Danvers, MA) diluted 2000-fold in 5% milk ...
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bioRxiv - Microbiology 2022Quote: ... and then incubated at room temperature with HRP-conjugated secondary antibodies ((goat anti-rabbit HRP conjugated, Cell Signaling, Cat #: 7074, 1:5,000 dilution) (goat anti-mouse HRP conjugated ...
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bioRxiv - Plant Biology 2022Quote: ... was analyzed for MAPK phosphorylation by immunoblotting using the primary antibody anti-pERK MAPK (Phospho-p44/p42 MAPK, ERK1/2,Thr202/Tyr204, D13.14.4E; Cell Signaling Technology, Danvers, MA, USA) at a dilution of 1:2,500 and the secondary antibody (Goat anti-rabbit IgG (H+L)-HRP conjugate ...
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bioRxiv - Neuroscience 2022Quote: ... and then immunoprecipitated for 3 hours at 4°C by agarose beads preincubated with rabbit anti-HTT D7F7 antibody (Cell Signaling, Cat#5656). To remove the non-specific binding ...
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bioRxiv - Neuroscience 2022Quote: ... blots were incubated for two hours in 5% milk in 1X TBST for blocking followed by an overnight incubation with rabbit anti-c-Fos antibody (1:1000, Cat. No. 2250, Cell Signaling Technology, USA) at 4°C ...
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bioRxiv - Physiology 2022Quote: ... for 1 hour at RT and then incubated O/N with primary antibody for c-Fos (Rabbit anti-cFos, Cell Signaling, Cat# 2250) in blocking solution at RT ...
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bioRxiv - Molecular Biology 2024Quote: All cargo constructs include an epitope tag or tandem tag (FLAG or 3xFLAG) for assay by anti-FLAG primary antibodies (Cell Signaling Technology, 2368). HEK293FT cells were transfected at 24 well ...
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bioRxiv - Cancer Biology 2024Quote: ... and incubated with anti-human N-Myc rabbit monoclonal antibody (#51705, Cell Signaling Technology, Inc., MA, USA at a dilution of 1:300) and S100 beta (EP32 ...
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bioRxiv - Cell Biology 2024Quote: ... the membranes were incubated overnight at 4°C with the following primary antibodies: anti-AMPKα (Cell Signaling Technology, USA; diluted at 1:1000), anti-p- AMPKα (Thr172 ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were later stripped by incubating in 0.5M NaOH for 10 min and then re-probed for total Akt levels by incubating with anti-Akt (C67E7) antibody (1:1000 dilution, Cell Signaling Technology 4691T). The density of each immunoreactive phospho-protein band was expressed as a fraction of the band for the total AKT protein in the same lane.
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bioRxiv - Immunology 2023Quote: ... Membranes were incubated for 1 h at room temperature with appropriate secondary antibodies: goat anti-rabbit (1:2000, Cell Signaling Technology, Cat. 7074), goat anti-rat (1:10000 ...
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bioRxiv - Synthetic Biology 2023Quote: ... Primary staining was performed on separate blots with the following antibody dilutions in 5% BSA/TBST: rabbit anti-EZH2 1:1000 (Cell Signaling Technology, 5246S), rabbit anti-CBX2 1:500 (Bethyl Laboratories ...
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bioRxiv - Cell Biology 2022Quote: ... the following primary antibodies were used: rabbit polyclonal anti-phospho-Ezrin (Thr567)/Radixin (Thr564)/Moesin (Thr558) at 1:250 (Cell Signaling Technology, #3141), rabbit polyclonal anti-phospho-Myosin Light Chain 2 (Ser19 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... anti-Rab11 (1:50; CST, C45B10, #3288) or anti-LAMP1 (1:100; CST, D2D11 XP, #9091) rabbit monoclonal antibodies were purchased from Cell Signaling Technology (CST). Alexa 488 goat anti-rabbit secondary antibody (1:1000 ...
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bioRxiv - Microbiology 2023Quote: ... Cells were then blocked with immunofluorescence (IF) block buffer (3% BSA in PBS) for 1 hour and incubated with primary antibodies: rabbit anti-γH2A.x (Cell Signaling 9718, 1:2000), rabbit anti-RPA32 (GeneTex GTX113004 1:2000) ...
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bioRxiv - Immunology 2022Quote: ... Cas9 expression was validated by flow cytometry using intracellular staining of cas9 protein with anti-cas9 monoclonal antibody (7A9-3A3, Cell Signaling, cat #48796). Cas9 activity was assessed by transducing the cas9-BV2 cells with pXPR_047 ...
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bioRxiv - Cell Biology 2023Quote: ... in PBS−/−] at room temperature for 1.5 h and subsequently incubated with primary rabbit anti-MPO antibody (Cell Signaling, clone: E1E7I, 1:500) and primary mouse anti-HLA-G antibody (BD ...
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bioRxiv - Genomics 2023Quote: ... Correct expression of the mAID-Dam-Lamin B1 fusion protein as well as the wildtype Lamin B1 protein was confirmed using Immunofluorescence staining using an anti-HA antibody at 1 in 500 dilution (Cell Signaling Technologies, C29F4) and an anti-LaminB1 antibody at 1 in 500 dilution (Abcam ...
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bioRxiv - Genetics 2022Quote: ... Neurons were subsequently blocked with 5% normal goat serum in 1x PBS for 1 h and then incubated with primary antibodies as follows: anti-GFP (Cell signaling, #2555S, R), anti-Gephyrin (1:500 ...