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Citations for Cell Signaling Technology :
5001 - 5050 of 10000+ citations for Mouse Anti Bovine Coronavirus Spike Antibody 5A4 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... samples were incubated with the following primary antibodies for 1 hour at RT: rabbit anti-PPAR-gamma 1:200 (Cell Signaling 2443S), mouse anti-SMA 1:300 (Sigma A5228 ...
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bioRxiv - Genetics 2021Quote: ... were incubated overnight at 4 degrees Celcius in a 1:1000 dilution with the following primary antibodies: anti-TP53 (Cell Signaling, 2524), anti-BRCA1 (Abclonal ...
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bioRxiv - Neuroscience 2021Quote: ... for 1 hour at room temperature before transferred to the anti-pH3 antibody solution (1:200 in blocking solution; #9706, Cell Signaling; RRID:AB_331748) at 4°C for 3 days ...
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bioRxiv - Cell Biology 2022Quote: ... Primary and secondary antibody incubations were done in 5% milk/PBS using rabbit anti-HA at 1:500 (Cell Signaling, Cat #: 3724S) and anti-Rabbit HRP at 1:10,000 (BioRad ...
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bioRxiv - Cancer Biology 2020Quote: ... The equivalent of 5 mg of protein was further enriched for phosphorylated tyrosine peptides by overnight immunoprecipitation (pY-IP) using a cocktail of anti-phosphotyrosine antibodies (4G10, Millipore; PT-66, Sigma;p-Tyr-100, Cell Signaling Technology).
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bioRxiv - Neuroscience 2019Quote: ... Cells then underwent immunocytochemical processing similar to that described above using primary antibodies against activated c-Jun-N-terminal kinase (rabbit anti-pJNK, 1:100, Cell Signaling Technology) or phosphorylated p38 MAPK (rabbit anti-p-p38 ...
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bioRxiv - Cancer Biology 2019Quote: ... the membranes were washed four times with TBST (5 minutes gentle rocking per wash) and incubated overnight with primary antibody Rabbit anti-Gli-1 (Cell signaling, #C68H3); 1:250 dilution in TBST-Milk at 4°C with gentle rocking ...
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bioRxiv - Developmental Biology 2019Quote: ... Embryos/explants were then blocked in PBS+ 10% FBS and 1% Triton X-100 and incubated overnight at 4°C with an anti-pSmad2/3 antibody (Cell Signaling #8828) at 1:1000 in block ...
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bioRxiv - Neuroscience 2019Quote: ... They were then incubated at 4°C overnight with the following primary antibodies diluted in buffer G: rabbit polyclonal anti-cleaved caspase-3 (Asp175) (Cell Signaling, #9661) at 1:250 ...
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bioRxiv - Biophysics 2019Quote: ... We also probed for the presence of eRF1 using Western blotting after the ribosome pelleting step (anti-eRF1 antibody from Cell Signaling Technology).
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bioRxiv - Systems Biology 2020Quote: ... we washed the cells 5 × 5 minutes with 5% BSA-PBS then incubated the cells at room temperature for 1 hour in 5% BSA-PBS containing 1:500 donkey anti-mouse secondary antibody conjugated to Cy3 (Jackson 715-165-150) and 1:500 goat anti-rabbit secondary antibody conjugated to Alexa Fluor 594 (Cell Signaling #8889). After the secondary incubation ...
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bioRxiv - Cell Biology 2020Quote: ... Antibody was added to each tube (10μL anti-Histone H3, Cell Signaling Technology, cat #4620; 4μg Normal Rabbit IgG, Cell Signaling Technology, cat #2729 ...
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bioRxiv - Cell Biology 2020Quote: ... Protein separation was achieved via SDS-PAGE and proteins were transferred to nitrocellulose for immunoblotting with the following primary antibody dilutions: rabbit monoclonal anti–Caveolin-1 (1:1000; Cell Signaling #3267), mouse monoclonal anti–Actin (1:1000 ...
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bioRxiv - Neuroscience 2020Quote: ... Gapdh was used as a loading control and was detected using a rabbit monoclonal anti-Gapdh antibody (1:2000, Cell Signaling Technology).
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bioRxiv - Neuroscience 2020Quote: ... sections were washed in PBS and incubated for 1h with A488-conjugated secondary goat anti-rabbit antibody (1:500, Cell Signaling, USA). Similar procedures were followed for POMC labelling in brain sections from POMC-CreERT2-Ai6 mice ...
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bioRxiv - Immunology 2020Quote: ... Membranes were blocked with 5% milk in 5% tris-buffered saline with 0.01% Tween-20 (TBST) for 1 hour at room temperature then incubated overnight with the primary antibodies anti-GAPDH (Cell Signaling Technology) and anti-IVNS1ABP (Atlas Antibodies) ...
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bioRxiv - Immunology 2020Quote: ... Membranes were then washed 3x with TBST at room temperature then incubated with secondary anti-rabbit HRP-conjugated antibody (Cell Signaling Technology) for 1 hour ...
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bioRxiv - Neuroscience 2021Quote: ... sections were incubated in a rabbit anti-HA monoclonal antibody (1:250 in blocking buffer; catalog no. 3724, Cell Signaling, Danvers, MA) for 48 h at 4°C ...
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bioRxiv - Bioengineering 2021Quote: ... Protein level could also be measured by immunofluorescence using fluorescently labeled antibodies (anti-Flag-Alexa 647, #15009; Cell Signaling, Danvers, MA, USA). These antibodies were flowed into the device and incubated with the immobilized proteins under the ‘button’ for 20 min at RT ...
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bioRxiv - Microbiology 2021Quote: ... The membranes were blocked with 5% non-fat milk/PBS for 30 min and incubated with primary antibodies as follows: anti-PDGFRA (Cell Signaling, 3174T), anti-COL1A1 (Proteintech ...
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bioRxiv - Microbiology 2020Quote: ... Membranes were blocked with 3% BSA or 5% milk and probed with the following primary antibodies-1:1000 rabbit anti-Flag (Cell Signaling technology); 1:100 chicken anti-E.histolytica CSN5 (custom) ...
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bioRxiv - Microbiology 2020Quote: ... Flare Biotech LLC) following by HRP conjugated goat anti-rabbit antibody (1:10,000 Ab in 3 % BSA in TBST; Cell Signaling Technology Inc). Images obtained were analyzed via ImageJ software.
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bioRxiv - Cancer Biology 2020Quote: ... Antibodies were first titrated on CD34+ fetal liver lysates and subsequently used at the following dilutions: Anti-GATA1 (Cell Signaling, 1:5), anti-STAG2 (Cell Signaling ...
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bioRxiv - Cancer Biology 2020Quote: ... Membranes were blocked in skim milk or BSA and probed overnight with the following antibodies: Anti-AML1 (Cell Signaling Technology, Cat# 4336); anti-acetyl-histone H3 (Millipore ...
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bioRxiv - Cell Biology 2021Quote: ... the cells were fixed with 4 % paraformaldehyde and 0.2 % picric acid in phosphate buffer (pH 6.9) for 30 minutes at room temperature and incubated with peroxidase-conjugated anti-rabbit antibody (Cell Signaling technology #7074) overnight at 4 °C ...
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bioRxiv - Cell Biology 2022Quote: ... Membranes were saturated and blots were probed with the following primary antibodies: 1:700 rabbit anti-βArr2 (Cell signaling Cat. No. 3857); 1:1000 rabbit anti-GSK3β (Cell signaling Cat ...
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bioRxiv - Cell Biology 2022Quote: Larval tissues were stained with standard immunohistochemical procedures using rabbit anti-Phospho-JNK polyclonal antibody (Cell Signaling Technology, Cat #4668, 1:100), chicken anti-β-galactosidase antibody (Abcam ...
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bioRxiv - Microbiology 2022Quote: ... cells were washed three times with PBS and incubated with a horseradish peroxidase (HRP)-conjugated anti-rabbit secondary antibody (Cell Signaling #7074) for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... except cells were cross-linked with formaldehyde (1%) for 5 min and cross-linked chromatin immunoprecipitated using an anti-HA rabbit monoclonal antibody (Cell Signaling Technology). Knock-in ESCs were first adapted to serum-free 2i/LIF condition as described before ChIP (15) ...
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bioRxiv - Physiology 2022Quote: ... antibody in 0.1% TBST containing 3% BSA overnight at +4°C followed by incubation with secondary HRP-linked anti-rabbit antibody (Cell Signaling, #7074). Immunoblotted proteins were detected with ProSignal Pico ECL Reagent (Genesee Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... membranes were washed for 10 minutes thrice with 1×TBST before incubation with horseradish peroxidase (HRP)-conjugated secondary antibodies (Goat Anti-Rabbit – Cell Signaling Technology, Danvers ...
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bioRxiv - Cancer Biology 2022Quote: The following antibodies were used for immunoblotting (IB) at 1:1,000 unless otherwise stated: Anti-AXL (8661S, Cell Signaling, IHC 1:500), anti-phospho AXL y702 (5724 ...
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bioRxiv - Molecular Biology 2022Quote: ... Peptides were enriched for acetylation with anti-acetyl antibody conjugated to agarose beads (Acetyl-Lysine Motif Kit; Cell Signaling Technology, Danvers, MA). This process was performed according to the manufacturer protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... Sections were blocked with 5% goat serum in tris-buffered saline and 0.1% Tween® 20 detergent (TBST) for 30 min and stained with anti-HER2 antibodies at 1:500 dilution (Cell Signaling #21650). After three washes with TBST ...
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bioRxiv - Cancer Biology 2022Quote: ... Endogenous peroxidase inhibition and blocking with normal horse serum was also carried out before the incubation with primary antibodies (anti-rabbit caspase3, 1:100, Cell signaling #9662), anti-mouse ki67 ...
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bioRxiv - Neuroscience 2020Quote: ... membranes were washed three times with 1xTBST before incubating with horseradish peroxidase (HRP)-conjugated secondary antibodies (Goat Anti-Rabbit – Cell Signaling Technology, Danvers ...
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bioRxiv - Neuroscience 2021Quote: ... the sections were incubated with a secondary antibody goat anti-rabbit conjugated to a horseradish peroxidase (HRP) (1:500, 7074S Cell Signaling Technology) during 2 hours at RT followed by a 10 minutes incubation at RT with TSA plus Biotin System (Biotin TSA 1:250 ...
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bioRxiv - Neuroscience 2021Quote: ... the sections were incubated with a secondary antibody goat anti-rabbit conjugated to a horseradish peroxidase (HRP) (1:500, 7074S Cell Signaling Technology) during 2 hours at RT followed by a 10 minutes incubation at RT with TSA Biotin System (Biotin TSA 1:100 ...
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bioRxiv - Biochemistry 2020Quote: ... The membranes were then incubated overnight at 4 °C with either rabbit anti-p-MEK antibody against Ser217/Ser221 (1:1000) (Cell Signaling, #9121), rabbit anti-p-ERK antibody against Thr202/Tyr204 (1:1000 ...
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bioRxiv - Cell Biology 2021Quote: ... The final concentrations of the primary and secondary antibodies and blocking solutions used in the brain sections are as follows: Rabbit anti-cFos (Cell signaling 2250S), donkey anti-rabbit 647 (Jackson ImmunoResearch 711-605-152 ...
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bioRxiv - Neuroscience 2022Quote: ... slide mounted sections were incubated overnight at room temperature with a rabbit anti-cFos monoclonal primary antibody (9F6, Cell Signaling, 1:8000), diluted in 0.1M phosphate buffer at pH 7.4 ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were further incubated with α-Syn rabbit polyclonal antibody 1:500 (US biological) followed by goat anti-rabbit secondary at 1:1000 (Cell signaling) after which treated with TMB substrate ...
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bioRxiv - Cancer Biology 2022Quote: ... Expression of γ-H2AX was measured by Western blotting using anti-γ-H2AX (Ser139, antibody # 2577; Cell Signaling Technology, Inc., Danvers, MA) as reported by us previously42,44,47.
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bioRxiv - Molecular Biology 2022Quote: ... the same amount of each cellular lysate was analyzed by gel electrophoresis and western blot using anti-WDR5 antibody (D9E1I; Cell Signaling #13105), anti-HSP90 antibody (F-8 ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Immunofluorescent staining was performed as previously described (Ibrahim et al 2019) and the following antibodies were used: rabbit anti-insulin (C27C9, 1:200, Cell Signaling #3014), mouse anti-insulin (L6B10 ...
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bioRxiv - Cell Biology 2019Quote: ... Samples were then incubated overnight on a plate rocker at 4° C in a 1:200 dilution of anti-phospho-Erk1/2 (Thr202/Tyr204) rabbit monoclonal antibody (Cell Signaling 4370) in blocking buffer ...
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bioRxiv - Immunology 2020Quote: ... GRP78) or anti-rabbit-HRP (TMPRSS2) conjugated secondary antibodies at 1:3000 for 2hrs at room temperature (Cell Signaling, Danvers, MA, USA). Visualization of TMPRSS2 ...
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bioRxiv - Systems Biology 2021Quote: ... one million cells from each library were stained with a 1:25 dilution of anti-HA AlexaFluor647 conjugated antibody (Cell Signaling Technology) in Buffer A (20 mM HEPES pH 7.5 ...
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bioRxiv - Neuroscience 2020Quote: ... 20 µg of total brain protein were loaded and anti-GFAP antibody (D1F4Q) XP Rabbit mAb (1:3000; Cell Signaling Technology, 12389), anti-Iba1 antibody (1:1000 ...
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bioRxiv - Neuroscience 2020Quote: ... membranes were washed three times with 1xTBST before incubating with horseradish peroxidase (HRP)-conjugated secondary antibodies (Goat Anti-Rabbit – Cell Signaling Technology, Danvers ...