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Citations for Cell Signaling Technology :
5101 - 5150 of 10000+ citations for Rat Isocitrate Dehydrogenase 1 Soluble IDH1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Neurons were then incubated for 1 h with either the rabbit anti-HA antibody (1:500; Cell Signaling #3724, RRID AB_1549585) or the mouse anti-myc antibody (1:500 ...
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bioRxiv - Neuroscience 2022Quote: ... and then overnight at 4°C with the primary antibody (mouse anti-β-ACTIN, 1:30,000, Sigma; rabbit anti-AKT1, 1:1000, Cell Signaling; rabbit anti-AKT2 ...
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bioRxiv - Physiology 2023Quote: ... Secondary antibodies were diluted in 5% blocking solution and membranes were incubated for 1 h at room temperature (1:1000 donkey anti-rabbit IgG, HRP-linked Antibody, #7074S, Cell Signaling). Proteins were detected based on HRP substrate-based enhanced chemiluminescence (#WBKLS0500 ...
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bioRxiv - Molecular Biology 2023Quote: ... blotted onto nitrocellulose membranes, and probed with anti-ZSWIM8 (1:400; Cat #PA5-59492, Thermo) or anti-GAPDH (1:1000; Cat #2118, Cell Signaling) followed by HRP-conjugated anti-rabbit secondary (1:10,000 ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were blocked with PBS-0.1% Tween 20 (PBS-T) containing 5% milk and were incubated with rabbit monoclonal anti-CYLD (1:500, #8462 Cell Signaling) and mouse monoclonal anti-β-actin (1:5,000 ...
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bioRxiv - Cancer Biology 2023Quote: ... phospho-H2AX (S139) (20E3) rabbit mAb (1:400 for standard immunofluorescence, 1:100 for FFPE tissue staining, Cell Signaling Technology #9718); p21 (12D1 ...
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bioRxiv - Developmental Biology 2023Quote: ... Blocking solution was replaced with 1° antibody (Ab) (Phospho-Smad1/5/9 Rabbit mAb #13820, Cell Signaling Technology 13820T, 1:300) in 10%NGS/PBS-Tx and the slides were incubated overnight at 4°C ...
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bioRxiv - Immunology 2023Quote: ... Membranes were blocked with TBS containing 1 % casein and 2 % BSA followed by incubation with primary antibodies (survivin 1:2000, 71G4B7, Cell Signaling; beta actin 1:5000 sc-4778 ...
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bioRxiv - Microbiology 2023Quote: ... 3% BSA overnight at 4 □C (rabbit α-LC3B 1:1000 – Cell Signaling/ #4108, rabbit α-Atg5 1:1000 – Cell Signaling/#2630 ...
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bioRxiv - Microbiology 2023Quote: ... A competitor solution was prepared by mixing soluble human IgG1-Fc fused Eph receptor/s (150 nM sEphB2-Fc or 1 μM sEphB3-Fc plus 1μM sEphB4-Fc) and anti-myc Alexa 647 (clone 9B11, 1/250 dilution, Cell Signaling Technology) in PBS-BSA ...
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bioRxiv - Cancer Biology 2023Quote: ... each membrane was probed with the appropriate horseradish peroxidase-conjugated secondary antibody (anti-mouse dilution 1:5,000; GTX213112-01, Gene Tex; anti-rabbit dilution 1:3000; 7074S, Cell Signaling Technology), and incubated for 1□h at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... the sections were labeled with mouse anti-GFP (1:500) antibody and rabbit anti-FOXP1 antibody (Cell Signaling Technology; 1:400) and detected by biotinylated secondary antibody/streptavidin-Alexa Fluor 488 (GFP ...
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bioRxiv - Immunology 2023Quote: ... 0,05% Tween-20 and 1% NGS in PBS) with diluted primary antibodies: (LSD1, 1:200, Cell Signaling Technology, Cat. No. 2184S), (MUC2 ...
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bioRxiv - Genomics 2023Quote: Antibodies against HA-Tag (3724S, 1:200 for immunofluorescence), citrate synthase (14309S, 1:1,000 for Western blot) were from Cell Signaling Technology; SLC25A12 (ab200201 ...
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bioRxiv - Cell Biology 2024Quote: ... WB 1:1000), LC3B (#2775, WB: 1:1000), GABARAPL1 (#26632, WB: 1:1000) and ATG4B (5299, WB :1000) were purchased from Cell Signaling. Beta-actin (A2228 ...
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bioRxiv - Neuroscience 2024Quote: ... and incubated with horseradish peroxidase-conjugated secondary IgGs (1:1,000 for rabbit #170-6515, mouse #170-6516, Bio-Rad, CA or 1:2,000 for #7076s, Cell signaling, MA), for 1hour at RT ...
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bioRxiv - Cell Biology 2024Quote: ... The following antibodies were used: p-PERK (Eli Lilly; 1:50) (73) and PERK (Cell Signaling Technology, 1:200, Cat. #3192).
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bioRxiv - Microbiology 2024Quote: ... Parasites were stained overnight with 13.1 mice monoclonal anti-P28 (1:1000) and rabbit anti-HA (1:250, Cell Signaling Technologies) antibodies ...
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bioRxiv - Cell Biology 2019Quote: ... 1D4, DSHB), mouse anti-Fasciclin III (1:100; 7G10, DSHB) and rabbit anti-phospho-JNK/SAPK (1:100; 81E11 Cell Signaling Technology). Appropriate secondary antibodies conjugated either with Cy3 (1:200 ...
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bioRxiv - Cell Biology 2020Quote: ... The Developmental Studies Hybridoma Bank at the University of Iowa) and Rabbit anti-cleaved Drosophila Dcp-1 (Asp216) (1:100; Cell Signaling Technologies). Secondary antibodies were used at a dilution of 1:500 ...
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bioRxiv - Cell Biology 2020Quote: ... The primary antibody against phosphorylated RSKS-1 is anti-phospho-p70 S6 kinase (Thr389) (1A5) Mouse mAb (Cell Signaling, #9206, 1:1,000), which detects the conserved residue Thr404 in worms and was tested for its specificity using wildtype and rsks-1 deletion mutant lysates ...
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bioRxiv - Cell Biology 2020Quote: ... samples were incubated with the following primary antibodies for 1 hour at RT: rabbit anti-PPAR-gamma 1:200 (Cell Signaling 2443S), mouse anti-SMA 1:300 (Sigma A5228 ...
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bioRxiv - Developmental Biology 2021Quote: ... the cells were washed with chilled FACS buffer (DPBS with 1% FBS and 2mM EDTA) and then incubated with the mouse anti-SSEA4 antibody (1: 50; 4755S, Cell Signaling Technology) on ice for 30 minutes ...
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bioRxiv - Neuroscience 2021Quote: ... Then the cells were blocked in 10% donkey serum for 2 h and incubated with primary antibodies (goat anti-Iba1, 1:400, Abcam; mouse anti-CD68, 1:400, Cell Signaling Technology; rabbit anti-Arg-1 ...
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bioRxiv - Neuroscience 2021Quote: ... for 1 hour at room temperature before transferred to the anti-pH3 antibody solution (1:200 in blocking solution; #9706, Cell Signaling; RRID:AB_331748) at 4°C for 3 days ...
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bioRxiv - Molecular Biology 2021Quote: ... the same amount of protein digestion products was subjected to Western blotting analysis probing with PEP7h antibody (1:5000) or Gapdh-HRP (D16H11, 1:5000, Cell Signaling Technology).
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bioRxiv - Neuroscience 2019Quote: ... 11A5(Anderson et al., 2006) (mouse monoclonal 11A5 kindly provided by Imago Pharmaceuticals, 1:25,000) and D1R1R (rabbit mAb #20706S, Cell signaling. 1:1000), NF-L (mouse mAb #2835 ...
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bioRxiv - Cell Biology 2019Quote: ... rabbit polyclonal SDHA (#11998, 1:1000) and rabbit monoclonal COXIV (3E11 clone, #4850, 1:1000) antibodies were purchased from Cell Signaling Technology. The donkey anti-rabbit (NA934 ...
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bioRxiv - Cell Biology 2019Quote: ... 1:100 for IF) and Rabbit anti-GFP (#2956, 1:100 for IF and 1:1000 for WB, respectively) were from Cell Signaling Technology.
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bioRxiv - Cell Biology 2020Quote: ... WB 1:1000), rabbit anti-TYRO3 (5585, WB 1:1000), rabbit anti-phospho-AXL (Tyr702) (5724, WB 1:1000) all from Cell Signaling Technology; mouse anti-α-tubulin (T5168 ...
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bioRxiv - Neuroscience 2020Quote: ... rabbit anti-ERK1/2 (dilution 1:2000) and rabbit α-tubulin (dilution 1:2000) all purchased from Cell Signaling Technology (Danvers, MA). The phospho-ERK1/2 expression was evaluated first using a 680-nm secondary anti-mouse antibody ...
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bioRxiv - Cell Biology 2020Quote: ... Protein separation was achieved via SDS-PAGE and proteins were transferred to nitrocellulose for immunoblotting with the following primary antibody dilutions: rabbit monoclonal anti–Caveolin-1 (1:1000; Cell Signaling #3267), mouse monoclonal anti–Actin (1:1000 ...
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bioRxiv - Biophysics 2021Quote: ... and stained with 1:50 RBD-sfGFP expression medium (prepared as previously described and 1:250 anti-myc Alexa 647 (clone 9B11, Cell Signaling Technology) in PBS-BSA ...
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bioRxiv - Bioengineering 2020Quote: ... and blocked with 1% BSA for 1 hour prior to incubation with primary antibodies raised against OCT4 (Cell Signaling, Cat. # 28901:800), NANOG (Cell Signaling ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were then stained with APC-conjugated CD45 (1:25 dilution; Miltenyi) and Alexa Fluor 488-conjugated pan-keratin (1:25 dilution, Cell Signaling Technology) antibodies for 10 min at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... anti-mCherry (AB0081; 1:300; SICGEN) for neuronal morphology and anti-phospho-Iϰβ-α (14D4 rabbit monoclonal antibody; 1:500; Cell Signaling) for labeling the AIS ...
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bioRxiv - Cell Biology 2021Quote: ... and Cleaved Caspase 3 (#9664, 1:400 dilution for IF) and anti-Rsk2 (#5528; 1:200 dilution for IF) were purchased from Cell Signaling Technology. Anti-Tubulin α (66031-1-Ig ...
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bioRxiv - Zoology 2021Quote: ... followed by sequential incubations at 4°C in one of the primary antibodies (1:1000 in blocking buffer) and an anti-rabbit HRP-conjugated secondary antibody (1:2000 in blocking buffer (Cell Signaling Technology). Visualization of identified total protein and phosphorylated protein bands was completed using an Amershan ECL Plus detection kit (GE Healthcare ...
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bioRxiv - Microbiology 2020Quote: ... 1:1,000, 9251), anti-c-jun (60A8, 1:1,000, 9165), anti-phospho-c-jun (S63, 1:1,000, 9261) (Cell Signaling, Beverly, MA, USA); anti-BECN1/Beclin-1 (1:1,000 ...
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bioRxiv - Microbiology 2019Quote: ... 1:3000 diluted in 1% BSA) and mouse monoclonal anti- HA (6E2) tag antibody (1:1000 dilution in 3% bovine serum albumin, Cell signaling, #2367) for 1hr at RT ...
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bioRxiv - Cell Biology 2021Quote: ... Membranes were washed three times with TBS-T buffer and incubated at RT for 1 hour with horseradish peroxidase-conjugated secondary anti-mouse or anti-rabbit antibodies (1:5000; Cell Signaling Technology). The membranes were washed three times in TBS-T ...
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bioRxiv - Cell Biology 2021Quote: ... from DSHB; anti-FoxO3 (1/1000; cat. no. 2497) and anti-phospho-Hsp27(Ser82) (1/1000; cat. no.2406) from Cell Signaling Technology; anti-HA (1/1000 ...
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bioRxiv - Cell Biology 2022Quote: ... and then subjected to western blot analysis using anti-Prdx5 (1:500, Ab Frontier) and anti-hnRNPK (1:500, Cell Signaling Technology) antibodies.
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bioRxiv - Cancer Biology 2022Quote: The following antibodies were used for immunoblotting (IB) at 1:1,000 unless otherwise stated: Anti-AXL (8661S, Cell Signaling, IHC 1:500), anti-phospho AXL y702 (5724 ...
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bioRxiv - Cell Biology 2022Quote: ... The membranes were incubated with 1:5 home-made anti-p53 (overnight at +4°C) and 1:100,000 anti-Lamin B1 (1 hr at room temperature) (Cell Signaling Technology, #12586). As secondary antibodies ...
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bioRxiv - Developmental Biology 2022Quote: ... 0.1% Tween-20 in PBS) for at least 1 h and then incubated in 1:100 anti-pSmad1/5/9 (Cell Signaling, #13820) in FBS-PBST at 4°C overnight ...
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bioRxiv - Cancer Biology 2019Quote: ... and incubated for 1 hour at 25°C with a solution containing 1/400 goat α-rabbit-Alexa-488 (Cell Signaling 4412S) and 1 μM Hoechst 34580 to detect nuclear material (H21486 Molecular Probes. ...
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bioRxiv - Cancer Biology 2020Quote: ... as manufacturer’s recommended protocol following overnight incubation with anti-PAX8 mouse antibody 1:250 (Novus: NBP2-29903) and anti-SOX17 rabbit antibody 1:250 (Cell Signaling: 81778S) at 4 °C ...
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bioRxiv - Microbiology 2022Quote: ... 1 μL of each substrate solution was added to 1 mL of PBS or RIPA lysis buffer (Cell Signaling Technology, Danvers, MA) for NLuc experiments ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were further incubated with α-Syn rabbit polyclonal antibody 1:500 (US biological) followed by goat anti-rabbit secondary at 1:1000 (Cell signaling) after which treated with TMB substrate ...