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Citations for Cell Signaling Technology :
5051 - 5100 of 10000+ citations for Rat Isocitrate Dehydrogenase 1 Soluble IDH1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2020Quote: ... The sections were cooled to room temperature and blocked with 20% fetal bovine serum for 45 minutes and then immunostained overnight with 1:100 dilution of primary antibody to PECAM-1 (Cell Signaling), SCF (Abcam) ...
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bioRxiv - Neuroscience 2019Quote: ... anti-histone H3 (Cat #4499P, 1:1000 dilution), and anti-histone H4 (Cat #13919P, 1:1000 dilution) were from Cell Signaling. β-actin antibody was from Sigma (Cat #A5441 ...
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bioRxiv - Neuroscience 2019Quote: ... Then the slices were incubated for 24 h at 4°C with primary antibodies, anti-mouse TH (dilution 1:1000, M318, millipore) and anti-rabbit c-Fos (dilution 1:200, 2250, Cell Signaling). The secondary antibodies ...
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bioRxiv - Synthetic Biology 2019Quote: ... Blots were incubated with primary antibodies (HA-Tag #2367, dilution 1:1000; myc-Tag #2276, dilution 1:1000; Cell Signaling Technology; Vincullin ab129002 ...
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bioRxiv - Cell Biology 2020Quote: ... which was performed as previously described (27,28) using the following antibodies: rabbit monoclonal anti-Caveolin-1 (1:100, Cell Signaling #3267); mouse monoclonal anti-Glutamine Synthetase (for Müller glia-specific marker ...
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bioRxiv - Cancer Biology 2020Quote: ... anti-β-catenin Ab (1:500) and anti-p-p53 (phosphorylated on S20) Ab (1:500) were purchased from Cell Signaling. Mouse anti-CA9 Ab (M75 ...
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bioRxiv - Neuroscience 2020Quote: ... The next day sections were washed in PBS and incubated for 1 h with A647-conjugated secondary goat anti-rabbit antibody (1:500, Cell Signaling). Sections were then mounted and cover-slipped.
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bioRxiv - Cell Biology 2020Quote: ... Cell pellets were loosed and resuspended by flick in 100 μl 1% BSA/PBS with 1 μl Alexa Fluor 488 conjugated phospho-Histone H3 (Ser10) antibody (DC28, Cell Signaling). After incubation at room temperature for 1 hour with occasional flick ...
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bioRxiv - Neuroscience 2020Quote: ... They were then incubated overnight at 4°C with the following primary antibodies diluted in PBS - 0.3% Triton X100 - 1% normal serum: rabbit anti-cleaved caspase 3 (1/400, Cell Signaling, 9661), rabbit anti-doublecortin (1/2000 ...
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bioRxiv - Neuroscience 2019Quote: ... and/or immunoblotting (IB) at the indicated dilutions: Rabbit anti-peIF2a (for PLA 1:6000 #44728G Invitrogen, and IB, 1:1000, Cell Signaling), mouse anti-eIF2a (IB ...
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bioRxiv - Molecular Biology 2021Quote: ... Mouse lung cryosections were stained overnight with anti-BrdU (1:3, BD Biosciences, or 1:1000 Cell Signaling Technology, Danvers, MA) and incubated with Alexa Fluo 488-conjugated secondary antibody (1:200 ...
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bioRxiv - Cancer Biology 2020Quote: ... For primary antibodies against [p] ERK (diluted 1:500) and total ERK (tERK; diluted 1:500; Cell Signaling Technology, Danvers, MA), antibodies were diluted in 0.1% TBST with 5% BSA ...
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bioRxiv - Cell Biology 2021Quote: ... 1:100 for Cut&Tag), DDX5 (9877T, 1:1,000 for WB), and RBBP5 (13171S, 1:200 for ChIP) from Cell Signaling Technology; PCBP2 (NBP2-19715 ...
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bioRxiv - Cell Biology 2021Quote: ... Primary antibodies used in the study were: anti-acetylated lysine (PTM Biolabs, 1:1000, and Cell Signaling Technology #9441, 1:1000), anti-malonylated lysine (PTM Biolabs ...
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bioRxiv - Biophysics 2020Quote: ... Immunoprecipitates were analyzed by Western blot as described above using the following antibodies: anti-HA rabbit polyclonal (1:1000) and anti-Shh rabbit monoclonal (1:1000, Cell signaling).
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Patient-associated mutations in Drosophila Alk perturb neuronal differentiation and promote survivalbioRxiv - Cancer Biology 2022Quote: ... Primary antibodies used: gp-anti-Alk (1:3000) (Loren et al., 2001) and rb anti-tubulin (1:5000; Cell Signaling, #11H10). After washing ...
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bioRxiv - Developmental Biology 2022Quote: ... Discs were incubated with primary antibodies overnight at 4°C: rabbit anti-Cleaved Drosophila cDcp-1 (1:200, Cell Signaling, #9578S), rabbit anti-fkh (1:200 ...
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bioRxiv - Cell Biology 2022Quote: ... cells were incubated with the following primary antibodies overnight: rabbit anti-RAD18 NB100-61063 (Novus) (1:200) and mouse anti-biotin (D5A7) (1:200) (Cell signaling). The rest of the protocol follows Proximity Ligation Assay as above.
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bioRxiv - Cell Biology 2021Quote: ... AMPK and pAMPK were stained using primary antibody polyclonal AMPK (1:1000, 2532, from cell signaling) and anti-phosphorylated AMPK (Thr 172) (1:1000, 2535, Cell Signaling), β-actin (1:2000 ...
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bioRxiv - Neuroscience 2021Quote: ... goat anti-chicken 488 (Life Technology A11039 at 1/200) and goat anti-rabbit 488 (Cell Signaling Technology #4412 1/200).
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bioRxiv - Neuroscience 2022Quote: ... rabbit anti-HA tag (C29F4) monoclonal antibody (1:1000 for NaV1.6 and 1:2000 for NF186; Cell Signaling, cat. no. 3724); mouse anti-ankyrin G antibody (1:50 ...
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bioRxiv - Pathology 2022Quote: ... Then membranes were incubated with peroxidase-conjugated secondary antibodies for 1 h at room temperature: anti-rabbit immunoglobulin G (IgG) (AB_2099233; Cat.#7074, 1:2000, Cell Signaling Technology), anti-mouse IgG (AB_330924 ...
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bioRxiv - Developmental Biology 2022Quote: Larval wing imaginal discs were stained using the following antibodies: Rabbit anti-cleaved Drosophila Dcp-1 (1:300, Cell Signaling Technology), Rabbit-anti-p-eIF2α (1:300 ...
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bioRxiv - Developmental Biology 2019Quote: ... mouse anti-Isl1/2 (Developmental Studies Hybridoma Bank, 1:100) and rabbit anti-β3-tubulin (Cell Signaling Technology, 5568S 1:200).
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bioRxiv - Immunology 2019Quote: ... The concentrated supernatant was mixed with SDS loading buffer for western blotting analysis with antibodies for detection of activated caspase-1 (D5782 1:500; Cell Signaling) or mature IL-1β (Asp116 1:500 ...
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bioRxiv - Neuroscience 2020Quote: ... including the primary antibodies (mouse anti-Iba1 antibody, Abcam, 1:400; rabbit anti-iNOS antibody, Abcam, 1:50; mouse anti-GFAP antibody, Cell signaling, 1:400 ...
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bioRxiv - Synthetic Biology 2021Quote: ... and incubated in 5% non-fat dry milk in 1× PBS and 0.1% Tween 20 with the primary antibodies (PHC1 Cell Signaling #13505 1:1000; Rybp Cell Signaling D8J7W 1:1000 ...
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bioRxiv - Microbiology 2020Quote: ... Nanobodies were incubated for 1 h at RT and detected using Mouse-anti-Myc antibody (1:1000, 9B11 clone, Cell Signaling). US28 was visualized with the rabbit-anti-US28 antibody (1:1000 ...
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bioRxiv - Immunology 2020Quote: ... The drying product was dissolved in 100 μl PBS and mixed with SDS loading buffer for western blotting analysis with antibodies for detection of activated caspase-1 (D5782 1:500, Cell Signaling), mature IL-1β (Asp116 1:500 ...
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bioRxiv - Microbiology 2019Quote: ... and then incubated for 1 h with 1:1,000 dilution of secondary HRP-conjugated goat anti-rabbit IgG antibody (Cell Signaling Technology). Images of the immunoblots were developed with ECLTM Western Blotting Detection Reagent (GE Healthcare).
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bioRxiv - Developmental Biology 2021Quote: ... and 0.05% Tween 20] plus 5% BSA before incubation with the primary antibody (1:1000 for mouse monoclonal 12C11 anti-REST and 1:2000 for anti-LaminB1 rabbit polyclonal, Cell Signaling). Anti-rabbit IgG or anti-mouse IgG conjugated to horseradish peroxidase (1:2000 ...
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bioRxiv - Cell Biology 2019Quote: ... which was used at a 1:50 dilution in combination with a secondary anti-mouse HRP antibody (1:10,000, Cell Signaling #7076S). ECL Select (GE Healthcare ...
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bioRxiv - Bioengineering 2020Quote: ... HRP conjugated goat anti-mouse (1:4000) and goat anti-rabbit (1:4000) secondary antibodies were obtained from Cell Signaling Technology, Danvers ...
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bioRxiv - Cell Biology 2019Quote: ... anti-Mena (1:300, mouse monoclonal, a kind gift from Frank Gertler) and anti-Cre Recombinase (1:300, Cell Signaling 12830). Secondary antibodies used were goat anti-mouse or anti-rabbit IgG conjugated to Alexa Fluor-488 ...
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bioRxiv - Physiology 2021Quote: ... Primary antibodies against p-Akt Ser473 (1:100 dilution; 4060) and p-AMPK Thr172 (1:200 dilution; 50081) from Cell Signaling were attempted ...
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bioRxiv - Physiology 2021Quote: ... anti-phospho and total IkB kinase antibody (p/total IkB 1:1000, 1:500, CST9246S, CST924, Cell Signaling Technology, Danvers, MA), anti-phospho and total panTrk (1:1000 ...
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bioRxiv - Microbiology 2021Quote: ... and 1:1000 dilution for c-Myc(c-Myc(9E10) Santacruz) and 1:1000 dilution for both Cek1/Mkc1(9102S Cell Signaling) and 1:1500 dilution for both phospho-Cek1/phosphor-Mkc1(4307T Cell Signaling) ...
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bioRxiv - Neuroscience 2022Quote: ... Then the sections were incubated with primary antibody of interest in the blockage solution (c-fos,1:200, Abcam/ab208942; NFκB-p65, 1:200, Cell Signaling Technology/8242 ...
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bioRxiv - Physiology 2022Quote: ... GAPDH-HRP (GTX627408-01, 1:120000) and P84 (GTX70220-01, 1:1000) The secondary antibodies were Anti-rabbit IgG (Cell Signaling, 7074 ...
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bioRxiv - Cell Biology 2022Quote: The following primary antibodies and concentrations were utilized: rabbit anti-Vimentin (Cell Signaling Technology, D21H3, WB 1:1000, IF 1:200), mouse anti-Vimentin (Invitrogen ...
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bioRxiv - Cancer Biology 2022Quote: ... #9188),Ki-67 (1:1,000 dilution; Novocastra; NCL-KI-67-P) and Cleaved Caspase 3 (1:200 dilution; Cell Signaling, #9661). p-CREB 1:100 dilution ...
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bioRxiv - Cancer Biology 2022Quote: ... The washed cells were then resuspended in 200 μL of PBS containing 1% BSA and 0.1% Triton X-100 (PBSTx/BSA) containing primary antibodies [MCM2 (1:200, D7G11, Cell Signaling Technology) and ψH2AX (1:200 ...
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bioRxiv - Cell Biology 2022Quote: ... The membrane was blocked with 5% non-fat dry milk in TBST and incubated with primary antibodies against Perilipin-2 (1:5000 gift from Dr. Ronal Kuhnlein) 13 and Actin (1:1000, Cell Signaling). The western blot images were captured by a BIO-RAD workstation.
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bioRxiv - Molecular Biology 2024Quote: ... anti-LC3A/B (D3U4C) XP (rabbit monoclonal, #12741, RRID: AB_2617131, Cell Signaling Technology; 1:200 for IHC, 1:2000 for WB), anti-Nwd1 (rabbit polyclonal generated via immunization with the recombinant mouse Nwd1 protein [18] ...
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bioRxiv - Cancer Biology 2024Quote: ... Primary antibodies include PKM1 and PKM2 (7067S, dilution 1:500, and 4053S, dilution 1:1000, respectively, Cell Signaling Technology, Danvers, MA), p63 and FOXA2 (ab735 and ab108422 ...
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bioRxiv - Cell Biology 2024Quote: ... following incubation with the primary antibodies anti-p53 (1:500, mouse, (Ab-6) Pantropic OP43 Sigma-Aldrich) and anti-GAPDH (1:1000, rabbit, 14C10 Cell Signaling) for 1h at room temperature ...
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bioRxiv - Biochemistry 2024Quote: ... Afterwards cells were incubated in blocking solution for 1h at 37 °C and incubated in the primary antibody (Cx36 1:2000, Chemicon, MAB3045; Sec24B 1:1000; Cell Signaling; Grasp55 1:2000 ...
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bioRxiv - Neuroscience 2023Quote: ... Respective expression was assessed by immunostaining using a mouse anti-HA antibody (1:500; Cell Signaling#2367, RRID AB_10691311 and a rabbit anti-Flag (1:500; Cell Signaling#14793 RRID AB_2572291 followed by Cy5-conjugated donkey anti-mouse (1.9 µg/ml ...
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bioRxiv - Neuroscience 2023Quote: ... Neurons were then incubated for 1 h with either the rabbit anti-HA antibody (1:500; Cell Signaling #3724, RRID AB_1549585) or the mouse anti-myc antibody (1:500 ...
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bioRxiv - Neuroscience 2022Quote: ... and then overnight at 4°C with the primary antibody (mouse anti-β-ACTIN, 1:30,000, Sigma; rabbit anti-AKT1, 1:1000, Cell Signaling; rabbit anti-AKT2 ...