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Citations for Cytoskeleton :
101 - 150 of 157 citations for WesternBright MCF IR fluorescent Western blotting kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2022Quote: ... The membrane was then probed with a mouse monoclonal anti-RhoA antibody (supplied with the Cytoskeleton Kit) at a working dilution of 1:500 overnight at 4 °C with constant agitation ...
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bioRxiv - Cell Biology 2023Quote: ... All samples were processed using the “G-actin / F-actin In Vivo Assay Kit” (Cytoskeleton Inc., #BK037) which involves lysing cells in a filament-supporting buffer ...
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bioRxiv - Cell Biology 2024Quote: ... and pten– cells was assessed using a pull-down assay kit (Cat. #BK008-S; Cytoskeleton, Denver, CO) following the manufacturer’s instructions as previously described (Kirolos and Gomer ...
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bioRxiv - Neuroscience 2024Quote: ... G- and F-actin was detected by G-Actin/F-Actin In Vivo Assay Biochem Kit (Cytoskeleton) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: Activated Rac1 was measured using the absorbance-based G-LISA Rac1 Activation Assay Biochem Kit (BK128, Cytoskeleton, USA). Briefly ...
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bioRxiv - Cell Biology 2019Quote: MT polymerization assays were performed using a fluorescence-based Tubulin Polymerization kit (Cytoskeleton Inc, Denver CO, Cat. # BK011P) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... cells lysates were prepared according to manufacturer’s instructions (RhoA Pull-Down Activation Assay Biochem Kit; Cytoskeleton Inc., BK036). Specifically ...
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bioRxiv - Microbiology 2020Quote: The microtubules binding experiments were performed with the Microtubule Binding Protein Spin-Down Assay Biochem Kit (Cytoskeleton, Inc.) following manufacturer protocol.
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bioRxiv - Microbiology 2022Quote: The levels of active GTP-bound Rac1 were measured by using Rac1 G-LISA kit (Cytoskeleton Inc., U.S.A) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were imaged post 24hr of transfection with SiR-actin (Cat. no. CY-SC001 Spirochrome kit, Cytoskeleton, Inc) staining in the complete media ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Active RhoA levels were measured using the RhoA Pull-down Activation Assay Biochem Kit (BK036-S, Cytoskeleton Inc.) using GST Rhotekin beads ...
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bioRxiv - Cancer Biology 2021Quote: RHOA activity was measured on LN-F lysates using the RHOA G-LISA Activation Assay kit (#BK124, Cytoskeleton). Data are the mean ± SEM of arbitrary units of RHOA activity.
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bioRxiv - Developmental Biology 2019Quote: Actin pelleting experiments were performed using the G-Actin/F-actin In Vivo Assay Biochem Kit (Cytoskeleton, Inc.) with modifications ...
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bioRxiv - Cell Biology 2021Quote: Cdc42 and Rac1 Pull-Down assays were carried out using Cdc42/Rac1 Activation Assay Biochem Kits (Cytoskeleton, inc), following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: Actin bundling assays were carried out using the Actin Binding Protein Spin-Down Assay Biochem Kit (Cytoskeleton Inc.) according to protocol of the manufacturer.
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bioRxiv - Cell Biology 2024Quote: ... Rho-GTP was measured using the RhoA G-LISA Activation Assay Kit (Colorimetric Format; Cat# BK124) from Cytoskeleton, according to the manufacturer’s protocol.
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bioRxiv - Cancer Biology 2021Quote: ... the Rac1- and cdc42 Small GTPase Activation Assay kits (G-LISA) were used (027BK127-S, 027BK128-S, Cytoskeleton, Inc) following the company’s guidelines.
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bioRxiv - Cell Biology 2020Quote: ... and G-LISA assay was performed as per the manufacturer’s instructions (Rac1 and Cdc42 G-LISA activation assay kits, Cytoskeleton).
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bioRxiv - Cell Biology 2021Quote: The pyrene-actin polymerization and depolymerization assays were performed using the Actin Polymerization Biochem Kit (Cytoskeleton, Inc., Denver, CO) following the manufacturer protocol ...
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bioRxiv - Systems Biology 2022Quote: The Rho activity was measured and quantified by using the RhoA Activation Assay Biochem Kit (cat. no. BK036; Cytoskeleton) based on the Rhotekin pull-down assay as per the manufacturer’s instructions as described previously(39) ...
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bioRxiv - Biochemistry 2022Quote: ... colorimetric Rac1 and RhoA G-LISA Activation Assay Kits (BK128-S and BK124-S Cytoskeleton Inc., Denver CO, USA) were used according to the manufacturer’s instructions.
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bioRxiv - Plant Biology 2021Quote: F-actin disruption assays were carried out using the Actin Binding Protein Spin-Down Assay Biochem kit (Cytoskeleton, USA) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: Actin protein levels were calculated using a G-actin/F-actin in vivo Assay Biochem Kit as per manufacturer’s instructions (Cytoskeleton). Briefly ...
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bioRxiv - Immunology 2021Quote: ... GTP-bound form of CDC42 was pulled down for analysis using the Cdc42 Pull-Down Activation Assay Kit (Cytoskeleton). FLAG-tagged protein pull-down assays were performed using DDDDK tagged protein purification kit (MBL) ...
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bioRxiv - Cancer Biology 2023Quote: GTP-bound RAS levels were assessed with a RAS G-LISA Assay Kit per manufacturer’s instructions (Cytoskeleton INC., #BK131). 9 ξ 105 cells were plated in a 3D 24-well plate ...
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bioRxiv - Cell Biology 2023Quote: ... GTP-bound ARF6 was detected in cell lysates using the ARF6 Activation Assay Biochem Kit™ (Cytoskeleton, BK-033) according to manufacturer’s instructions.
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bioRxiv - Cell Biology 2020Quote: For the determination of active GTP bound form of Cdc42 we used the G-LISA kit for Cdc42 from Cytoskeleton according to the protocol of the manufacturer.
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bioRxiv - Biophysics 2020Quote: ... Rac1-GTP and Cdc42-GTP using the RhoA and Cdc42 pull-down activation assay biochem kit (PN:BK036-S and BK034-S, Cytoskeleton, Inc.) following the protocol provided by the manufacturer ...
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bioRxiv - Cancer Biology 2020Quote: ... Lysate preparation and measurement of RHOA-GTP levels were carried out with the RHOA G-LISA activation assay kit (#BK124, Cytoskeleton) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2022Quote: G- and F-actin were fractionated and enriched using a commercial in vivo assay biochem kit (Cytoskeleton Inc, Colorado, USA), according to the manufacturer’s instructions with minor modifications ...
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bioRxiv - Molecular Biology 2020Quote: ... Intracellular Rac1 activity was determined using a Rac1 G-LISA Activation Assay Kit (Colorimetric format) (Cytoskeleton, catalog BK128, Denver, CO) using 25μg total protein per the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... Intracellular RhoA activity was determined using a RhoA G-LISA Activation Assay Kit (Colorimetric format) (Cytoskeleton, catalog BK124, Denver, CO) using 20μg total protein per the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... In vitro microtubule-binding assay was performed using the microtubule-binding protein spin down assay kit (Cytoskeleton, Denver, CO, USA) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: The protocol of G and F actin measurement mostly followed the instructions of the “G-actin /F-actin In Vivo Assay Kit” from Cytoskeleton Inc ...
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bioRxiv - Cell Biology 2022Quote: ... and Rac or Rho activity was assessed in duplicate using the G-LISA small G-protein activation assay kit (BK128, BK124, Cytoskeleton) according to the manufacturer’s directions ...
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bioRxiv - Microbiology 2023Quote: ... IP of total SUMOylated protein fractions used either SUMO-1 or SUMO-2/3 Ab included with the Signal Seeker SUMOylation 1 or 2/3 Detection Kit (Cytoskeleton) and IPs were carried out according to the manufacturer instructions ...
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bioRxiv - Cell Biology 2023Quote: RhoAGTP and Rac1GTP pull down as-says were performed using the RhoA and Rac1 Activation Assay Biochem Kit (Cytoskeleton BK030) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2021Quote: Activation of ARF6 (ARF6-GTP) was quantitated using the Arf6 Pull-Down Activation Assay Biochem Kit from Cytoskeleton (BK033-S, USA) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2019Quote: G/F-actin assay was performed according to the manufacturer’s instructions (G/F-actin In Vivo Assay Kit, Cytoskeleton, Denver, CO). G-and F-actin bands on western blots were scanned by densitometry and the ratios of free G-actin to F-actin were calculated.
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bioRxiv - Microbiology 2022Quote: ... 110 μl of LAS2 buffer was prepared according to the protocol of a G-actin/F-actin In Vivo Assay Biochem Kit (Cytoskeleton, Inc.) and mixed with the pellet ...
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bioRxiv - Cell Biology 2023Quote: The F-actin/G-actin ratio was measured using G-Actin/F-Actin In Vivo Assay Biochem Kit (#BK037, Cytoskeleton, Inc.), following the manufacturer’s instructions.
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bioRxiv - Neuroscience 2023Quote: F- and G-actin fractions from lysates were prepared using a G-Actin/F-Actin in Vivo Assay Biochem kit (Cytoskeleton, #BK037) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: Western blots to determine the ratio of G-actin to F-actin were performed on retinal lysates that were processed using a G-actin/F-actin In Vivo Assay Kit (Cytoskeleton, Inc.) following the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2023Quote: ... NIH3T3 cells were imaged using four wavelengths (405 nm for tagBFP; 488 nm for Citrine; 561 nm for mCherry or FusionRed; 674 nm for SiR700-Tubulin Kit, #CY-SC014, Cytoskeleton Inc.).
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bioRxiv - Neuroscience 2024Quote: Active GTP-bound GTPase levels in brain lysates were measured using G-LISA activation assay kits for Rac1 (Cytoskeleton, Inc., BK128) and RhoA (Cytoskeleton ...
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bioRxiv - Cell Biology 2022Quote: ... cells were incubated for 30 min with 0.5 μM of SiR-actin or 60 min with 0.5 μM of SiR-tubulin and 10 μM of verapamil in culture medium (SiR Cytoskeleton Kit, Spirochrome SC006).
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bioRxiv - Neuroscience 2020Quote: ... and RAC1 were performed through the use of an ELISA-based Rho-GTPase activation assay kit (Cytoskeleton, Inc, catalog# BK124, BK127, BK128). Briefly ...
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bioRxiv - Neuroscience 2020Quote: RhoA activation was assessed with the G-LISA activation kit in accordance with the manufacturer’s instructions (Cytoskeleton Inc., catalog no. BK124-S). Active GTP-bound RhoA was detected with HRP-conjugated anti-RhoA antibodies ...
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bioRxiv - Cell Biology 2023Quote: Measurements of the microtubule-stimulated ATPase activities of KIF1A MD-NC-CC1-FHA and various mutants were performed by using the HTS Kinesin ATPase Endpoint Assay Biochem Kit (Cytoskeleton, Inc., BK053). Briefly ...
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bioRxiv - Cancer Biology 2023Quote: ... Total protein was extracted from multiple cell cultures (G411, G729, G797) using the RAC1 Activation Assay Biochem Kit per manufacturer’s instructions (Cytoskeleton Inc, #BK035-S) five days post transduction with lentiviral non-targeting or GJB2 shRNA ...