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Citations for Cytoskeleton :
1 - 50 of 157 citations for WesternBright MCF IR fluorescent Western blotting kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... The pull-down protein samples were then subjected to western blotting with the whole cell lysate and His-tagged Rac1 protein (#RC01, Cytoskeleton) serving as the controls.
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bioRxiv - Cancer Biology 2021Quote: ... GTP-pulldown and western blot procedures were performed according to RHOA/RAC1/CDC42 Activation Assay Combo kit (Cytoskeleton) manufacturer’s protocol ...
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bioRxiv - Bioengineering 2020Quote: ... Acti-stain™ Fluorescent Phalloidin (Cytoskeleton, Inc.) and DAPI (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... Green Fluorescent Fibronectin (Cytoskeleton, Inc HiLyte 488) was used to determine gel functionalisation in Supplementary Fig ...
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bioRxiv - Cell Biology 2021Quote: ... The laminin density was evaluated by coating the dish with laminin conjugated to a fluorescent dye (green fluorescent HiLyte 488; Cytoskeleton), which was observed under a confocal laser scanning microscope (Zeiss LSM710 ...
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bioRxiv - Neuroscience 2019Quote: ... Actin-stain 555 Fluorescent Phalloidin was from Cytoskeleton. The siRNA against CaMKIIγ was from GenePharma (Shanghai ...
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bioRxiv - Developmental Biology 2024Quote: ... or Acti-stain 488 fluorescent Phalloidin (Cytoskeleton, Inc.) in PBST for 20 min ...
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Rab35 Governs Apicobasal Polarity Through Regulation of Actin Dynamics During Sprouting AngiogenesisbioRxiv - Developmental Biology 2022Quote: Globular and filamentous actin ratios were determined by western blot as described by commercially available G-actin/ F-actin In Vivo Assay Kit (Cytoskeleton). Globular and filamentous immunocytochemistry was performed as previously described [53] ...
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bioRxiv - Cell Biology 2021Quote: ... and 6% fluorescent (X-Rhodamine or HiLyte647) tubulin (Cytoskeleton Inc) in the presence of the nonhydrolyzable GTP analogue GMPCPP (Jena Bioscience ...
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bioRxiv - Microbiology 2023Quote: ... 100 nM Acti-stain 488 Fluorescent Phalloidin (Cytoskeleton, Denver, CO, USA), and 300 nM DAPI (Merck ...
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bioRxiv - Microbiology 2023Quote: ... and ∼5% Alexa488-labeled or Alexa647-labeled fluorescent tubulin (Cytoskeleton inc.) were prepared as described64 and stabilized with final 20µM taxol (Cytoskeleton inc.) ...
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bioRxiv - Molecular Biology 2020Quote: ... and incubated with Acti-Stain 670 fluorescent phalloidin (Cytoskeleton #PHDG1, 70 nM) and 4’,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Cell Biology 2022Quote: ... Taxol-stabilized microtubules with ∼10% biotin-tubulin and ∼10% fluorescent-tubulin (Cytoskeleton) were prepared as described previously51 ...
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bioRxiv - Cell Biology 2022Quote: ... mouse monoclonal anti-CDC42 (Cytoskeleton Inc., ACD03, 1:250 for Western blot); mouse monoclonal anti-Rac1 (Merck Millipore ...
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bioRxiv - Cell Biology 2022Quote: ... osteoclasts were incubated with live imaging compatible fluorescent probes: SIR-Actin (Cytoskeleton, Inc.), LysoTrackerTM Red DND-99 (Thermo Fisher) ...
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bioRxiv - Cell Biology 2019Quote: ... media was replaced with Fluorobright fluorescent imaging media containing 500 nM sirDNA (Cytoskeleton). The chamber was then transferred to a Nikon Ti microscope fitted with an OKOlab environmental control chamber ...
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bioRxiv - Microbiology 2023Quote: ... we used Rhodamine-labelled Phalloidin or Acti-stain 488 Fluorescent Phalloidin (Cytoskeleton, Inc), per manufacturer instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... a highly specific fluorescent dye for F-actin (1:250, Cytoskeleton, Inc., CY-SC001), for 3 hours at room temperature ...
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bioRxiv - Cell Biology 2021Quote: Fluorescent microtubules were polymerized by incubating 20µM porcine brain tubulin and 2µM TMR-tubulin (Cytoskeleton) with 1mM DTT and 1mM GTP in BRB80 (80mM PIPES pH 6.8 ...
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bioRxiv - Cancer Biology 2019Quote: ... Equal protein levels were confirmed by a control western blot of rabbit anti-Kinesin (Cytoskeleton) from the cleared lysates ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were analyzed by western blot using anti-ubiquitin antibody (P4D1, Cytoskeleton AUB01-HRP, 1:1000) and visualized with Clarity Western ECL Substrate (BioRad).
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bioRxiv - Cell Biology 2021Quote: Fluorescent MTs were polymerized at 2 mg/ml by incubating tubulin and rhodamine labeled tubulin (Cytoskeleton Inc.) at 10:1 ratio in BRB80 with 1 mM DTT and 1mM GTP for 5 min on ice ...
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bioRxiv - Cell Biology 2024Quote: ... The cell-adhesive patterns were confirmed by coating with green fluorescent-HiLyte 488-Laminin (Cytoskeleton, #LMN02-A) (Extended Data Fig ...
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bioRxiv - Cell Biology 2023Quote: ... The samples were stored at −20°C prior to western blot analysis for actin (Cytoskeleton 1:1000). Standards (10 ...
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bioRxiv - Cancer Biology 2019Quote: ... Cells were stained in suspension with a 1:300 dilution of Anti-Stain 488 Fluorescent Phalloidin (Cytoskeleton, #PHDG1), gently mixed ...
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bioRxiv - Biophysics 2022Quote: ... The PDMS replicates were then coated with 50 μM fibronectin in PBS (Cytoskeleton, Inc. Green Fluorescent, HiLyte 488).
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bioRxiv - Bioengineering 2023Quote: ... we incubated the glass coverslip with a 500 µg/mL solution of green fluorescent laminin (Cytoskeleton, Inc., LMN02) for 2 hours at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... and secondary antibody at 1 h (4 °C) facilitated by an automated western blot liquid handler (Cytoskeleton GOBlot). Antibodies utilised included anti-eIF2α (abcam ...
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bioRxiv - Cancer Biology 2021Quote: ... F-actin and G-actin were quantified by SDS-PAGE and western blot using actin antibody (#BK037, Cytoskeleton Inc.).
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bioRxiv - Neuroscience 2021Quote: ... washed and the immunoprecipitated resolved on 12% SDS-PAGE and detected via Western blot analysis using Cdc42 (mouse anti-Cdc42, ACD03 Cytoskeleton; 1:250), Rac1 (mouse anti-Rac1 ...
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bioRxiv - Neuroscience 2021Quote: ... The samples were resolved on 12% SDS-PAGE and detected via Western blot analysis using actin (rabbit anti-actin, BK037 Cytoskeleton Inc.; 1:500) and visualized by ECL (Pierce ...
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bioRxiv - Biophysics 2021Quote: ... the cells were rinsed thoroughly and stained with 1:200 solution of secondary antibody in PBS for 2 hours followed by staining with Acti-Stain™ 670 Fluorescent Phalloidin (Cytoskeleton.Inc, catalog no. PHDN1). Then the cover-slides were mounted on glass slides using ProLong Diamond Antifade Mountant (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: Actin Polymerization Biochem Kit (Cytoskeleton) was used to investigate F-actin depolymerization by cofilin in the absence or presence of control protein BSA ...
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bioRxiv - Biophysics 2022Quote: ... provided along with the kit (Cytoskeleton) was used to detect G- and F-actins ...
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bioRxiv - Cancer Biology 2021Quote: Rac1 and RhoA GTPase actitation was measured by G-LISA Rac1 activation assay Kit and G-LISA RhoA activation Kit (Cytoskeleton), according to manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: ... assays were carried out using kits from Cytoskeleton, Inc ...
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bioRxiv - Cell Biology 2020Quote: Activated RhoA pulldown kits were purchased from Cytoskeleton. Sample lysates were prepared similar to the protocol used for G-LISAs ...
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bioRxiv - Physiology 2022Quote: ... the Cdc42 activation kit (Cytoskeleton Inc., Denver, USA) was used according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: Rac1 activation was measured by using a Rac1 activation assay kit (Rac1 Pull-down Activation Assay Biochem Kit bead pulldown format, Cytoskeleton, catalog #BK035), following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: Commercial ELISA kits were purchased from Cytoskeleton (G-LISA) to quantify RhoA and Rac1 activation (Cytoskeleton) ...
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bioRxiv - Cancer Biology 2022Quote: RAS Activation Assay Biochem Kit™ (BK008; Cytoskeleton, Inc) was used to assess KRAS activity following manufacturer’s instructions ...
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bioRxiv - Biophysics 2020Quote: ... the microtubules were labeled by SiR700-tubulin Kit (Cytoskeleton) at a final concentration of 800 nM in cell culture medium for 2 h before imaging.
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Membrane-dependent actin polymerization mediated by the Legionella pneumophila effector protein MavHbioRxiv - Molecular Biology 2023Quote: ... Actin polymerization biochem kit (BK003) was purchased from Cytoskeleton Inc ...
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bioRxiv - Cancer Biology 2022Quote: ... the RAS-GTP pull-down assay kit (Cytoskeleton #BK008) was used ...
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bioRxiv - Cancer Biology 2023Quote: ... G-LISA RhoA or Rac1 activation kits from Cytoskeleton were used according to the manufacturer’s protocol.
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bioRxiv - Cell Biology 2020Quote: ... the Cd42 G-LISA kit (Cytoskeleton Inc., Denver, CO, USA) was used according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... For ubiquitin pulldown assays using the SignalSeeker kit (Cytoskeleton Inc), cells were lysed in provided BlastR buffer with protease inhibitor and NEM ...
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bioRxiv - Genomics 2024Quote: ... and 500 nM SiR-tubulin Kit (Cytoskeleton, Denver, Colorado, U.S.) according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2019Quote: ... colorimetric G-LISA activity assay kit (BK128-S, Cytoskeleton, Denver, Colorado) was used according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2019Quote: Bulk Rac1 activity was measured using an assay kit (BK035, Cytoskeleton), as previously described (Hara et al. ...