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Citations for Cytoskeleton :
101 - 150 of 341 citations for WAS Protein Family Member 3 WASF3 Antibody Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2020Quote: ... or 3 μg/mL C3 transferase (Cytoskeleton, Inc, Denver, CO).
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bioRxiv - Cell Biology 2020Quote: ... 3 G-LISA™ Activation Assay (purchased from Cytoskeleton Inc.), according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... 20 nM bovine brain Arp2/3 complex (Cytoskeleton; cat#RP01P), and 20 nM GST-WASP were mixed with purified Fbp17SH3 ...
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bioRxiv - Systems Biology 2020Quote: ... 300µg of total cell protein was used to pull-down GTP-bound RAS/RAF-RBD complexes according to the manufacturer’s instructions (Cytoskeleton). Activated RAS or 20µg of total cell protein were separated using NuPAGE Novex Bis-Tris gels (Invitrogen/Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... Lysates were spun at maximum speed for 10mins before protein level was measured using Precision Red (Cytoskeleton, Inc., #ADV02). Samples were run using precast 4-12% NuPAGE Bis-Tris Acrylamide Gels (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: Purified tubulin proteins were obtained from Cytoskeleton, Inc ...
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bioRxiv - Developmental Biology 2022Quote: ... Protein lysates were centrifugated for 15 min at > 15000 rpm at 4°C and supernatant was collected and quantified by Precision Red Advanced Protein Assay (Cytoskeleton). 5-10 μg of protein were boiled in NuPAGE LDS sample buffer (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: ... In vitro microtubule-binding assay was performed using the microtubule-binding protein spin down assay kit (Cytoskeleton, Denver, CO, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: Muscle homogenates were prepared in buffer A as described above and an equal amount of protein from each sample was incubated with 20 µL of Rhotekin-RBD beads (Cytoskeleton) for 2 hours at 4°C and washed 4 times in ice-cold buffer containing 50 mM Tris (pH 7.4) ...
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bioRxiv - Cell Biology 2022Quote: ... and Rac or Rho activity was assessed in duplicate using the G-LISA small G-protein activation assay kit (BK128, BK124, Cytoskeleton) according to the manufacturer’s directions ...
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bioRxiv - Cell Biology 2020Quote: ... the membrane was probed using the iBind flex kit and a primary anti-RhoA antibody (Cytoskeleton) at 1:200 and HRP-conjugated secondary (1:4000) ...
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bioRxiv - Neuroscience 2023Quote: ... The pull-down protein samples were then subjected to western blotting with the whole cell lysate and His-tagged Rac1 protein (#RC01, Cytoskeleton) serving as the controls.
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bioRxiv - Molecular Biology 2020Quote: ... 160 nM WASP VCA domain protein (#VCG03, Cytoskeleton) and 60 nM Arp2/3 protein complex (#RP01P ...
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bioRxiv - Cancer Biology 2022Quote: ... Precision Red Advanced Protein Assay (ADV02-A; Cytoskeleton), calcein AM (C1430 ...
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bioRxiv - Cell Biology 2021Quote: ... 1 μM TRITC-labeled tubulin protein (Cytoskeleton, TL590M) and 1 mM GTP (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2021Quote: Protein concentrations were determined by Bradford (Cytoskeleton, USA). Twenty μg of protein was resolved on polyacrylamide-SDS gels and electro-transferred to PVDF membranes.
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bioRxiv - Biophysics 2022Quote: ... The membrane was then probed with a mouse monoclonal anti-RhoA antibody (supplied with the Cytoskeleton Kit) at a working dilution of 1:500 overnight at 4 °C with constant agitation ...
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bioRxiv - Microbiology 2019Quote: ... 5 μL of the filtered supernatant was pipetted into a clear flat-bottom UV plate and 250 μL of 1x Advanced Protein Assay Reagent (Cytoskeleton Inc., CO) was added and mixed ...
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bioRxiv - Plant Biology 2019Quote: ... and GST-tagged VCA domain of human WASP protein (Cytoskeleton) were used to initiate actin nucleation ...
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bioRxiv - Cell Biology 2023Quote: The Rhotekin-RBD Beads (Binds Active Rho Proteins) from Cytoskeleton Inc ...
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bioRxiv - Microbiology 2022Quote: ... around 3× 107 trophozoites were incubated in cell lysis buffer (Cytoskeleton Inc, Colorado, USA) with vigorous agitation at 4°C for 30 min and homogenized by a 23-gauge needle on a 5-ml syringe ...
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bioRxiv - Cell Biology 2021Quote: ... MT affinity/dead-head spin was performed by binding motor protein to newly polymerized MTs (unlabeled, 2uM Paclitaxel (Taxol (Cytoskeleton, Denver, CO, US, TXD01) -stabilized ...
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bioRxiv - Developmental Biology 2021Quote: ... Membranes were incubated in primary antibody diluted in TBST (1:500 anti-RHOA antibody, Cytoskeleton ARH04) or diluted in block (1:30,000 anti-a-alpha tubulin antibody ...
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bioRxiv - Neuroscience 2023Quote: ... then rinsed and sequentially incubated with primary antibodies to RhoA followed by HRP secondary antibodies (Cytoskeleton), and the colorimetric reaction was performed for 15 min at 37 °C ...
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bioRxiv - Biophysics 2021Quote: The following cytoskeleton proteins are purchased: porcine brain tubulin (Cytoskeleton T240), HiLyte Fluor-488-labeled tubulin (Cytoskeleton TL488M-A) ...
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bioRxiv - Cell Biology 2020Quote: ... reserving a small amount for protein quantification using Precision Red (Cytoskeleton). Prior to measuring GTPase activity ...
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bioRxiv - Cell Biology 2021Quote: Soluble tubulin polymerization mix containing 50 μM tubulin protein (Cytoskeleton, T240), 1 μM TRITC-labeled tubulin protein (Cytoskeleton ...
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bioRxiv - Cell Biology 2021Quote: ... 22 μl of 5 mg ml−1 tubulin protein (Cytoskeleton, T240) was mixed with 2 μl of 5 mg ml−1 TRITC-labeled tubulin protein (Cytoskeleton ...
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bioRxiv - Biochemistry 2023Quote: ... and protein concentrations were estimated using the Precision Red reagent (Cytoskeleton Inc). Protein samples were prepared by mixing 1X LDS (Invitrogen) ...
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bioRxiv - Biophysics 2021Quote: ... the buffer also contained 50 µM non-muscle human actin protein (Cytoskeleton, Inc) and 0.2 mM ATP and 0.2 mM CaCl2.
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bioRxiv - Cell Biology 2021Quote: ... A mix of 20 μM of porcine brain tubulin protein (Cytoskeleton/Tebu-Bio) with 1 mM GMPCPP (Jena Bioscience ...
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bioRxiv - Biophysics 2020Quote: 0.4 µM Myosin Motor protein S1 Fragment (Rabbit Skeletal Muscle, Cytoskeleton #CS-MYS04) was incubated either alone or with 2 µM UCS proteins for 10 minutes either at 42°C or on ice in Mg-buffer (10mM Hepes-KOH ...
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Deletion or inhibition of PTPRO mitigates diet-induced hepatic steatosis and inflammation in obesitybioRxiv - Immunology 2022Quote: ... and Tyr-phosphorylated proteins were collected using Anti-phosphotyrosine Affinity Beads (Cytoskeleton, USA). Proteins were concentrated using the TCA/acetone precipitation method (Fic et al. ...
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bioRxiv - Biochemistry 2024Quote: ... we used a MT binding protein spin-down assay kit (# BK029, Cytoskeleton, Inc.) according to the manufacturer’s instructions ...
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bioRxiv - Pathology 2023Quote: ... MT assays were performed using the MT Binding Protein Spin-down kit from Cytoskeleton, Inc ...
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bioRxiv - Cell Biology 2023Quote: Actin binding and polymerization were assessed using the actin binding protein kit (Cytoskeleton, BK013). Actin pellet gel bands were quantified by densitometry using FIJI.
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bioRxiv - Biophysics 2021Quote: ... Increasing concentrations of tropomyosins (0.2-3.5 μM) were mixed and incubated with 3 μM rabbit skeletal-muscle F-actin (Cytoskeleton, Inc.) in T buffer at a final reaction volume of 50 μL for 1 h at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... Monoclonal Mouse anti-RhoA antibody (Cytoskeleton inc. Cat#ARH05) was used at 1:500 dilution in TBST overnight.
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bioRxiv - Cell Biology 2020Quote: ... Monoclonal Mouse anti-RhoA antibody (Cytoskeleton inc. Cat#ARH05) was obtained through the Cytoskeleton inc Rho A activation Assay Biochem Kit and used at 1:500 ...
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bioRxiv - Immunology 2022Quote: ... Monoclonal Rac1 antibodies (Cat. # ARC03) were purchased from Cytoskeleton.
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bioRxiv - Immunology 2022Quote: Monoclonal Rac1 antibodies (Cat. # ARC03) were purchased from Cytoskeleton.
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bioRxiv - Cancer Biology 2019Quote: ... Equal protein levels were confirmed by a control western blot of rabbit anti-Kinesin (Cytoskeleton) from the cleared lysates ...
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bioRxiv - Microbiology 2020Quote: ... recombinant human cofilin-1 (CFL1 P23528) and arp2/3 complex (ARPC4 Q148J6) from bovine brain were all purchased from Cytoskeleton Inc ...
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bioRxiv - Bioengineering 2022Quote: ... The beads were cleaned 3 times in PBS and resuspended in a solution of PBS with 20 μg/ml biotinylated fibronectin (Cytoskeleton) or biotinylated BSA (Pierce ...
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bioRxiv - Neuroscience 2023Quote: ... or Cdc42 activation in vivo assay biochemical kits for the respective protein (Cytoskeleton Inc., Denver, CO). The tissues were lysed in lysis buffer (50mM Tris-HCl pH7.4 ...
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bioRxiv - Biochemistry 2024Quote: ... The cells were lysed and protein lysates were used for G-LISA analysis per manufacturer’s (Cytoskeleton) instructions ...
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bioRxiv - Bioengineering 2019Quote: HUVECs were seeded on the PEG islands and control SiO2 substrates for 3 h and then flooded with cell culture medium containing 1:1000 dilution of SiR-DNA (Cytoskeleton Inc). An additional hour was required for cells to uptake SiR-DNA to be trackable through SiR-DNA probe using Leica DMI6000 microscope ...
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CDK5 activity in retinal pigment epithelium contributes to gap junction dynamics during phagocytosisbioRxiv - Cell Biology 2023Quote: ... the samples were preincubated for either 3 h with Rho/Rac/Cdc42 Activator I (1 µg/ml, Catalog number CN04, Cytoskeleton Inc), 30 min with MEK inhibitor PD 98059 (50 µM ...
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bioRxiv - Cell Biology 2020Quote: Primary antibodies used were rabbit anti-actin polyclonal (Cytoskeleton, Denver, CO) and mouse anti–MYO-3 monoclonal (5–6 ...
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bioRxiv - Neuroscience 2021Quote: ... incubated with a polyclonal antibody against actin (1:500; Cytoskeleton Inc.) and developed by chemiluminescence ...