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Citations for Cytoskeleton :
51 - 100 of 341 citations for WAS Protein Family Member 3 WASF3 Antibody Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... Protein concentration was measured with Precision Red Assay (Cytoskeleton). Laemmli sample buffer (Biorad ...
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bioRxiv - Cancer Biology 2022Quote: ... Protein was quantified using the Precision Red (#ADV02; Cytoskeleton) advanced protein assay and 10-20μg was used ...
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bioRxiv - Genetics 2024Quote: ... Protein concentration was determined using APA assay (Cytoskeleton Inc.) and 50 - 100 μg total protein was separated on SDS-PAGE followed by western blot ...
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bioRxiv - Cell Biology 2023Quote: ... Protein concentration of the cleared lysates was determined by Precision Red Advanced Protein Assay Reagent (Cytoskeleton Inc.). Equal amounts of total cell lysates were loaded to 4–12% Bis-Tris or 3–8% Tris-Acetate gels (Invitrogen ...
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bioRxiv - Cell Biology 2019Quote: ... Microtubule seeds containing 10% X-rhodamine-labeled and 10% biotin-labeled tubulin (Cytoskeleton Inc.) were generated by polymerization in the presence of the non-hydrolysable GTP analogue GMPCPP (NU-405S ...
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bioRxiv - Biochemistry 2022Quote: ... paclitaxel-stabilised MTs containing 10% X-rhodamine-labelled and 10% biotin-labelled tubulin (Cytoskeleton) were polymerized as above and were left at room temperature for 48 hours before use in the TIRF assay.
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bioRxiv - Cell Biology 2022Quote: ... 18% biotin-labeled porcine tubulin and 12% rhodamine-labeled porcine tubulin (all from Cytoskeleton) was incubated with 1 mM GMPCPP (Jena Biosciences ...
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bioRxiv - Genetics 2024Quote: ... Protein quantification was determined via Bradford assay using PrecisionRed Advanced Protein Assay reagent (#ADV01, Cytoskeleton, Denver, CO, USA). Lysate concentration was normalized before dilution in 2X Laemmli Sample buffer with 10% v/v 2-mercaptoethanol ...
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bioRxiv - Cell Biology 2023Quote: ... and total protein concentration was measured using Precision Red (Cytoskeleton). Normalized samples were denatured with SDS loading buffer (250mM Tris [pH 6.8] ...
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bioRxiv - Biophysics 2019Quote: Unlabeled and labeled (biotin and TRITC) lyophilized tubulin from porcine brain were purchased from Cytoskeleton Inc (Denver ...
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bioRxiv - Plant Biology 2019Quote: The VCA domain of human WASP protein was expressed in a bacterial expression system as a GST-tagged fusion protein (Cytoskeleton). The seven protein subunits of the Arp2/3 protein complex were purified from bovine brain (Cytoskeleton) ...
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bioRxiv - Cell Biology 2024Quote: ... Soluble proteins were collected after a 10 minute centrifugation step at 15000 rpm and protein concentration was measured using PrecisionRed (Cytoskeleton; ADV02). 1.5 mg of protein was mixed with 25 μl of pre-equilibrated GFP-Trap_A beads (ChromoTek ...
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bioRxiv - Genetics 2024Quote: ... Protein concentration was determined using APA assay (Cytoskeleton Inc. Denver, CO, USA) and 50 μg total protein were separated on SDS-PAGE followed by western blot ...
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bioRxiv - Cell Biology 2023Quote: ... biotin-and HiLyte-488-labeled pig brain microtubules were polymerized in vitro using commercial tubulin sources (Cytoskeleton Inc.) and stabilized with taxol (Sigma Aldrich ...
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bioRxiv - Cancer Biology 2023Quote: ... Biotin-labeled tubulin as well as HiLyte647-labeled tubulin and TAMRA Rhodamine labeled tubulin were purchased from Cytoskeleton Inc ...
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bioRxiv - Cell Biology 2020Quote: ... Arp2/3 complex (Cytoskeleton, Inc. RP01P), and GST-WASP-VCA (Cytoskeleton ...
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bioRxiv - Cell Biology 2019Quote: ... 50 nM Arp2/3 (Cytoskeleton Inc.), 1 μM VCA (Cytoskeleton Inc. ...
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bioRxiv - Biophysics 2020Quote: ... 3 μM Rhodamine tubulin (TL590M, Cytoskeleton), and 3 mM GMPCPP (NU-405S ...
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bioRxiv - Cell Biology 2023Quote: ... 50 nM Arp2/3 (Cytoskeleton; RP01P), 1 μM VCA domain of WASP (Cytoskeleton ...
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bioRxiv - Cell Biology 2019Quote: ... stabilized microtubule seeds were prepared by polymerizing 50 μM porcine tubulin containing biotin-labeled (1:14.5) and rhodamine-labeled (1:14.5) porcine tubulins (Cytoskeleton, Inc.) in the presence of 1 mM GMPCPP at 37 °C for 30 min ...
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bioRxiv - Cell Biology 2021Quote: GMPCPP-stabilized microtubules were assembled using 50 μM unlabeled porcine tubulin containing 1:12 biotin-labeled porcine tubulin (Cytoskeleton) and polymerized in the presence of 1mM GMPCPP (Jena Bioscience ...
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Gα13 loss promotes tumor progression in the KPC transgenic mouse model of advanced pancreatic cancerbioRxiv - Cancer Biology 2021Quote: ... and the protein concentration was determined using Precision Red solution (Cytoskeleton, Inc., Denver, CO) according to the manufacturer’s instructions ...
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bioRxiv - Pathology 2023Quote: ... and the protein concentration was determined using Precision Red solution (Cytoskeleton, Inc., Denver, CO). Equal amounts of protein were separated with a 10% SDS-PAGE electrophoresis gel ...
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bioRxiv - Cell Biology 2020Quote: ... Mouse anti-SUMO2/3 (#12F3, Cytoskeleton Inc), Guinea Pig anti-SUMO2/3 (1:300 ...
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bioRxiv - Cell Biology 2019Quote: ... Mouse anti-SUMO2/3 (#12F3, Cytoskeleton Inc), and Guinea Pig anti-SUMO2/3 (1:300 ...
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bioRxiv - Cell Biology 2021Quote: ... GMPCPP-stabilized microtubule seeds were assembled using 50 μM porcine tubulin containing 1:12 biotin-labeled and 1:10 rhodamine-labeled porcine tubulins (Cytoskeleton) and polymerized in the presence of 1mM GMPCPP (Jena Bioscience ...
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bioRxiv - Cell Biology 2021Quote: ... Tubulin was labeled in 1:10 proportions with biotin or X-rhodamine according to published protocols or mixed with prelabeled tubulin purchased from Cytoskeleton, Inc.
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bioRxiv - Cell Biology 2022Quote: The Microtubule Binding Protein Spin-down assay was performed according to the manufacturers’ protocol (Cytoskeleton). Briefly ...
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bioRxiv - Cancer Biology 2023Quote: ... The isolation of ubiquitinated proteins was performed with the Signal-Seeker Ubiquitin Enrichment kit (Cytoskeleton). Cell fractionation was performed with the Qproteome Cell Compartment kit (Qiagen).
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bioRxiv - Biochemistry 2020Quote: ... was added to chambers and incubated for 8 minutes followed by 8 minutes with 50−150 nM biotin inactivated myosin (Cytoskeleton, Inc), both prepared in 50 mM Tris pH 7.5 ...
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bioRxiv - Biophysics 2022Quote: ... GMPCPP-stabilized, biotinylated microtubule seeds were assembled from bovine brain tubulin (Castoldi & Popov, 2003) and porcine biotin tubulin (Cytoskeleton, Cat #T333), and incubated on the coverslip for 5 min before washing with growth buffer (1 mM GTP in BRB80 80 mM PIPES ...
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bioRxiv - Cell Biology 2020Quote: ... Actin (2µM; 7% pyrene-labeled) was then polymerized in the presence of 20nM bovine Arp2/3 complex (Cytoskeleton Inc.) plus MBP-tagged fusion proteins in control buffer supplemented with 0.2mM ATP and 0.5mM DTT ...
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bioRxiv - Cell Biology 2023Quote: ... a reaction vessel was prepared on ice containing 3 mg/ml of >97% purified porcine tubulin (Cytoskeleton, Inc. (#T240)) and the substances being tested ...
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bioRxiv - Microbiology 2023Quote: ... IP of total SUMOylated protein fractions used either SUMO-1 or SUMO-2/3 Ab included with the Signal Seeker SUMOylation 1 or 2/3 Detection Kit (Cytoskeleton) and IPs were carried out according to the manufacturer instructions ...
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bioRxiv - Cell Biology 2021Quote: ... was mixed with 2 μl of 5 mg ml−1 TRITC-labeled tubulin protein (Cytoskeleton, TL590M) and 1 μl of 5 mg ml−1 Biotin-XX-labeled tubulin (Pursolutions ...
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bioRxiv - Cell Biology 2024Quote: ... The protein was then transferred to a clean tube and quantified using PrecisionRed (Cytoskeleton; ADV02-A) at OD600.
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bioRxiv - Biophysics 2019Quote: ... Porcine Arp2/3 complex is purchased from Cytoskeleton and used with no further purification ...
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bioRxiv - Biophysics 2021Quote: ... and Arp2/3 complex were purchased from Cytoskeleton Inc ...
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bioRxiv - Cell Biology 2024Quote: ... anti-SUMO2/3 (Asm23, Cytoskeleton, 1:200 dilution), and anti-PML (sc966 ...
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bioRxiv - Cell Biology 2022Quote: ... Protein concentrations were determined using the Precision Red Advanced Protein Assay Reagent (Cytoskeleton ADV02). Extracted proteins (25-35 μg ...
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bioRxiv - Cell Biology 2019Quote: ... and protein concentration determined using the Precision Red™ Advanced Protein Assay Reagent (Cytoskeleton). Respective samples were treated with 300nM Nocodazole for 15 min before lysis ...
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bioRxiv - Cell Biology 2022Quote: ... or along with the human recombinant proteins: WASP-VCA domain protein (Cat# VCG03, Cytoskeleton) or ERK3 protein (M31-34G ...
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bioRxiv - Cell Biology 2020Quote: Purified actin protein (Cytoskeleton, Inc.) was used for the in vitro actin polymerization reaction ...
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bioRxiv - Neuroscience 2020Quote: ... and protein concentration estimated in the supernatant using Precision Red Advanced Protein Assay (Cytoskeleton, CA). Samples were prepared at 25 µg protein/well with 2X laemmli loading buffer and 2-mercaptoethanol and run on pre-cast 4-15% gels (Biorad) ...
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bioRxiv - Immunology 2023Quote: ... Protein concentrations of crude extracts were determined using the Advanced Protein Assay (Cytoskeleton, Denver, USA).
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bioRxiv - Plant Biology 2020Quote: The actin polymerization modulation activity of the proteins was checked using Actin Polymerization Biochem Kit (Cytoskeleton, USA), using the manufacturer’s instruction ...
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bioRxiv - Biophysics 2024Quote: ... GST-tagged WCA domain of human WASP protein (GST-WCA) was purchased from Cytoskeleton (Cat. #VCG03-A) and used without further purification ...
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bioRxiv - Cell Biology 2019Quote: ... and protein concentrations were measured using the Precision Red Advanced Protein Assay Reagent (# ADV02; Cytoskeleton Inc.) and finally equalized with ice-cold lysis buffer to 1.0 mg/ml ...
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bioRxiv - Cell Biology 2019Quote: ... Equalized protein extractions were transferred to a Rho-GTP-binding protein pre-coated plate (Cytoskeleton, Inc.). The plate was placed on a microplate shaker at 300 rpm for 30 min at 4°C ...
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bioRxiv - Developmental Biology 2019Quote: ... C3 exoenzyme protein (CT03; Cytoskeleton, Inc.) was resuspended and dialyzed into PBS and injected at 1 mg/mL ...