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Citations for Cytoskeleton :
101 - 150 of 215 citations for Human E3 Ubiquitin Protein Ligase SMURF2 SMURF2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2020Quote: ... 300µg of total cell protein was used to pull-down GTP-bound RAS/RAF-RBD complexes according to the manufacturer’s instructions (Cytoskeleton). Activated RAS or 20µg of total cell protein were separated using NuPAGE Novex Bis-Tris gels (Invitrogen/Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... Lysates were spun at maximum speed for 10mins before protein level was measured using Precision Red (Cytoskeleton, Inc., #ADV02). Samples were run using precast 4-12% NuPAGE Bis-Tris Acrylamide Gels (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: Rac1 activation was measured by using a Rac1 activation assay kit (Rac1 Pull-down Activation Assay Biochem Kit bead pulldown format, Cytoskeleton, catalog #BK035), following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: RAS Activation Assay Biochem Kit™ (BK008; Cytoskeleton, Inc) was used to assess KRAS activity following manufacturer’s instructions ...
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bioRxiv - Biophysics 2020Quote: ... the microtubules were labeled by SiR700-tubulin Kit (Cytoskeleton) at a final concentration of 800 nM in cell culture medium for 2 h before imaging.
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Membrane-dependent actin polymerization mediated by the Legionella pneumophila effector protein MavHbioRxiv - Molecular Biology 2023Quote: ... Actin polymerization biochem kit (BK003) was purchased from Cytoskeleton Inc ...
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bioRxiv - Cancer Biology 2022Quote: ... the RAS-GTP pull-down assay kit (Cytoskeleton #BK008) was used ...
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bioRxiv - Cancer Biology 2023Quote: ... G-LISA RhoA or Rac1 activation kits from Cytoskeleton were used according to the manufacturer’s protocol.
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bioRxiv - Developmental Biology 2022Quote: ... Protein lysates were centrifugated for 15 min at > 15000 rpm at 4°C and supernatant was collected and quantified by Precision Red Advanced Protein Assay (Cytoskeleton). 5-10 μg of protein were boiled in NuPAGE LDS sample buffer (Invitrogen ...
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bioRxiv - Cancer Biology 2021Quote: ... F-actin and G-actin protein samples were collected according to the manufacturer’s instructions (#BK037, Cytoskeleton Inc., Denver, CO, USA). F-actin and G-actin were quantified by SDS-PAGE and western blot using actin antibody (#BK037 ...
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bioRxiv - Cell Biology 2020Quote: Muscle homogenates were prepared in buffer A as described above and an equal amount of protein from each sample was incubated with 20 µL of Rhotekin-RBD beads (Cytoskeleton) for 2 hours at 4°C and washed 4 times in ice-cold buffer containing 50 mM Tris (pH 7.4) ...
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bioRxiv - Developmental Biology 2023Quote: ... Supernatant was collected upon 15min of centrifugation at >15000 rpm at 4°C and quantified by Precision Red Advanced Protein Assay (Cytoskeleton). Equal amounts of protein were boiled in NuPAGE LDS sample buffer (Invitrogen ...
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bioRxiv - Cell Biology 2020Quote: ... the Cd42 G-LISA kit (Cytoskeleton Inc., Denver, CO, USA) was used according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... and 500 nM SiR-tubulin Kit (Cytoskeleton, Denver, Colorado, U.S.) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2019Quote: ... 5 μL of the filtered supernatant was pipetted into a clear flat-bottom UV plate and 250 μL of 1x Advanced Protein Assay Reagent (Cytoskeleton Inc., CO) was added and mixed ...
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bioRxiv - Cell Biology 2019Quote: ... colorimetric G-LISA activity assay kit (BK128-S, Cytoskeleton, Denver, Colorado) was used according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2019Quote: Bulk Rac1 activity was measured using an assay kit (BK035, Cytoskeleton), as previously described (Hara et al. ...
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bioRxiv - Neuroscience 2022Quote: ... G-LISA RhoA activation assay biochem kit (Absorbance based) (Cytoskeleton, BK124) was used according to the manufacturer’s protocol ...
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bioRxiv - Biophysics 2022Quote: The RhoA Activation Assay Biochem Kit (bead pull-down format, Cytoskeleton) was used for performing the pull-down assay on 2D monolayers that were either uninduced or induced for the (DN ...
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bioRxiv - Cell Biology 2023Quote: RhoA GAP assays were performed using a kit provided by Cytoskeleton Inc (Denver Colorado Cat no ...
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bioRxiv - Biochemistry 2022Quote: ... The integrated signal measured from the area of the dynein bands is extracted using ImageJ and calibrated using known masses of purified dynein protein (CS-DN01, Cytoskeleton, Denver, CO, USA) that serve as standards ...
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bioRxiv - Cell Biology 2021Quote: ... MT affinity/dead-head spin was performed by binding motor protein to newly polymerized MTs (unlabeled, 2uM Paclitaxel (Taxol (Cytoskeleton, Denver, CO, US, TXD01) -stabilized ...
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bioRxiv - Cell Biology 2020Quote: ... a commercial kit was used following the manufacturer’s instructions (027BK034-S, Cytoskeleton). Briefly ...
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bioRxiv - Bioengineering 2019Quote: ... transfected cells were stained by SiR-DNA nuclear staining kit (Cytoskeleton. Inc) for 2 hours before imaging ...
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bioRxiv - Biophysics 2022Quote: The G-actin/F-actin In Vivo Assay Kit (cat. #BK037, Cytoskeleton) was used to determine the F/G-actin ratio ...
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bioRxiv - Bioengineering 2022Quote: ... For F-actin staining by SiR-Actin Kit (#CY-SC001, Cytoskeleton, Inc.), the staining solution was prepared by diluting SiR-actin and verapamil (a broad-spectrum efflux pump inhibitor ...
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bioRxiv - Cell Biology 2022Quote: ... ubiquitination enrichment immunoprecipitation was performed using Signal-Seeker Ubiquitination Detection kit (Cytoskeleton) as per the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: For Airy-SIM imaging: We use SiR Tubulin Kit (Cytoskeleton, CY-SC002) to label tubulin in live COS-7 cells under the conconcentration of 1μM ...
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bioRxiv - Cancer Biology 2022Quote: RAS activity was analyzed with a Ras Activation Assay Biochem Kit (Cytoskeleton, BK008). The QGY cell lines containing the control group ...
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bioRxiv - Cell Biology 2022Quote: Actin polymerization assay was performed using actin polymerization biochem kit (Cat# BK003, Cytoskeleton) according to the provided protocol ...
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bioRxiv - Neuroscience 2023Quote: ... and the Cdc42 pull-down activation assay kit (#BK034) were purchased from Cytoskeleton, Inc ...
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bioRxiv - Cell Biology 2023Quote: The high throughput screen (HTS) tubulin polymerization assay kit (Cat. BKOO4P, Cytoskeleton Inc.) was carried out as per the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... a Ras GTP-ase immunosorbent assay (G-LISA) Activation Assay Kit (#BK131, Cytoskeleton) was used ...
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bioRxiv - Cell Biology 2023Quote: In vitro actin polymerization was assessed using the actin polymerization kit (Cytoskeleton, BK003). Briefly ...
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bioRxiv - Bioengineering 2023Quote: ... Actin fibers were stained adding Sir-Actin and Verapamil (Spirochrome Cytoskeleton Kit - SC006) in the primary droplet media at respectively 1 µM and 10 µM ...
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bioRxiv - Neuroscience 2024Quote: ... RhoA activity was detected using the RhoA G-LISA Activation Assay Kit (Cytoskeleton) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2019Quote: ... a G-LISA RhoA Activation Assay Biochem kit (# BK124; Cytoskeleton Inc., Denver, CO, USA) was used according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2020Quote: The microtubule polymerisations were all performed with reagents from the kit purchased from Cytoskeleton, Inc ...
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Autocrine Sfrp1 inhibits lung fibroblast invasion during transition to injury induced myofibroblastsbioRxiv - Cell Biology 2022Quote: Activation of RhoA was quantified using the RhoA G-LISA assay kit (Cytoskeleton Inc.) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... we used the RhoA/Rac1/Cdc42 Activation Assay Combo Biochem Kit (Cytoskeleton, Inc., BK030). The pull-down was performed following manufacturer’s instructions.
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bioRxiv - Cancer Biology 2022Quote: We performed Rac1–GTP pull-down assay using the Rac1 activation kit (# BK035-S, Cytoskeleton) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: RhoA activities were determined using G-LISA™ RhoA Activation Assay Biochem Kit™ (Cytoskeleton) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... For microtubules specific fractionation we used a microtubules/tubulin in vivo assay kit (Cytoskeleton, #BK038) according to vendor specifications ...
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bioRxiv - Cancer Biology 2023Quote: RAC1 activation was examined using the Cdc42 Activation Assay Biochem Kit (BK034, Cytoskeleton, Denver, USA) following them anufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... The following steps were performed using the Cytoskeleton Ras-activation assay biochem kit (Cytoskeleton, #BK008) according to the manufacturer’s instructions ...
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Mapping chromatin state and transcriptional response in CIC-DUX4 undifferentiated round cell sarcomabioRxiv - Cancer Biology 2023Quote: The RAS GST-RBD activation kit was obtained from Cytoskeleton (Denver, CO, USA; cat #BK008). The protocol was performed following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... we used the G-actin/F-actin in vivo assay kit (Cytoskeleton Inc., Denver, USA) according to manufacturer’s protocol ...
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bioRxiv - Bioengineering 2024Quote: ... and samples were collected using the “Microtubules/Tubulin In Vivo Assay Kit” (Cytoskeleton, Cat. #BK038) as per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: For GTP-RhoA pull down assay the Rho Activation Assay biochem kit (Cytoskeleton inc, Cat# BK036) was used as described in the product manual ...
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bioRxiv - Cell Biology 2020Quote: Rho activation assay was performed using a RhoA Pull-down Activation Assay Biochem Kit from Cytoskeleton Inc (BK036-S) ...