Labshake search
Citations for Cytoskeleton :
51 - 100 of 215 citations for Human E3 Ubiquitin Protein Ligase SMURF2 SMURF2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genetics 2024Quote: ... Protein quantification was determined via Bradford assay using PrecisionRed Advanced Protein Assay reagent (#ADV01, Cytoskeleton, Denver, CO, USA). Lysate concentration was normalized before dilution in 2X Laemmli Sample buffer with 10% v/v 2-mercaptoethanol ...
-
bioRxiv - Cell Biology 2020Quote: Purified tubulin proteins were obtained from Cytoskeleton, Inc ...
-
bioRxiv - Biochemistry 2024Quote: ... Taxol-stabilized human unmodified microtubules assembled with 1.5% biotinylated tubulin (Cytoskeleton, Inc. #T333P) and 1% HiLyte FluorTM 488-labeled tubulin (Cytoskeleton ...
-
bioRxiv - Microbiology 2019Quote: ... Whole colonic tissue was homogenized in cell lysis buffer and protein concentration was determined using the Precision Red Protein Assay (Cytoskeleton). Samples were sent to Eve Technologies for analysis using their 32-multiplex Mouse Cytokine/Chemokine Array.
-
bioRxiv - Neuroscience 2023Quote: ... The pull-down protein samples were then subjected to western blotting with the whole cell lysate and His-tagged Rac1 protein (#RC01, Cytoskeleton) serving as the controls.
-
bioRxiv - Molecular Biology 2020Quote: ... 160 nM WASP VCA domain protein (#VCG03, Cytoskeleton) and 60 nM Arp2/3 protein complex (#RP01P ...
-
bioRxiv - Microbiology 2021Quote: ... Protein concentration was determined using Precision Red (Cytoskeleton) and 60μg of protein were separated using 4-15% SDS-PAGE gels and transferred onto nitrocellulose membrane ...
-
bioRxiv - Cancer Biology 2022Quote: ... Precision Red Advanced Protein Assay (ADV02-A; Cytoskeleton), calcein AM (C1430 ...
-
bioRxiv - Cell Biology 2021Quote: ... 1 μM TRITC-labeled tubulin protein (Cytoskeleton, TL590M) and 1 mM GTP (Sigma-Aldrich ...
-
bioRxiv - Molecular Biology 2021Quote: Protein concentrations were determined by Bradford (Cytoskeleton, USA). Twenty μg of protein was resolved on polyacrylamide-SDS gels and electro-transferred to PVDF membranes.
-
bioRxiv - Developmental Biology 2022Quote: ... Protein concentration was quantified using Precision Red (Cytoskeleton) according to manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2024Quote: ... Soluble proteins were collected after a 10 minute centrifugation step at 15000 rpm and protein concentration was measured using PrecisionRed (Cytoskeleton; ADV02). 1.5 mg of protein was mixed with 25 μl of pre-equilibrated GFP-Trap_A beads (ChromoTek ...
-
bioRxiv - Molecular Biology 2020Quote: ... and 60 nM Arp2/3 protein complex (#RP01P, Cytoskeleton) were also added to the actin polymerization buffer ...
-
bioRxiv - Biochemistry 2020Quote: ... was measured using Advanced Protein Assay Reagent (Cytoskeleton, Inc.) with bovine serum albumin as a standard ...
-
bioRxiv - Developmental Biology 2021Quote: ... Protein concentration was determined using APA assay (Cytoskeleton Inc.) and 50 μg total protein were separated on SDS-PAGE followed by western blot ...
-
bioRxiv - Cell Biology 2020Quote: ... Protein concentration was measured with Precision Red Assay (Cytoskeleton). Laemmli sample buffer (Biorad ...
-
bioRxiv - Cancer Biology 2022Quote: ... Protein was quantified using the Precision Red (#ADV02; Cytoskeleton) advanced protein assay and 10-20μg was used ...
-
bioRxiv - Genetics 2024Quote: ... Protein concentration was determined using APA assay (Cytoskeleton Inc.) and 50 - 100 μg total protein was separated on SDS-PAGE followed by western blot ...
-
bioRxiv - Biochemistry 2020Quote: ... For measurements including actin (human platelet actin, 99% pure, from Cytoskeleton, Inc., catalog nr: APHL99), the buffer was 5 mM Tris ...
-
bioRxiv - Plant Biology 2019Quote: ... The purified Arp2/3 protein complex from porcine brain (Cytoskeleton) and GST-tagged VCA domain of human WASP protein (Cytoskeleton ...
-
bioRxiv - Cell Biology 2023Quote: ... and total protein concentration was measured using Precision Red (Cytoskeleton). Normalized samples were denatured with SDS loading buffer (250mM Tris [pH 6.8] ...
-
bioRxiv - Cell Biology 2023Quote: The Rhotekin-RBD Beads (Binds Active Rho Proteins) from Cytoskeleton Inc ...
-
bioRxiv - Cell Biology 2019Quote: ... and then incubated with monoclonal mouse anti-human RhoA primary antibody (# GL01A; 1:250; Cytoskeleton Inc.), followed by a polyclonal goat anti-mouse horseradish-conjugated secondary antibody (# GL02 ...
-
bioRxiv - Biophysics 2021Quote: The following cytoskeleton proteins are purchased: porcine brain tubulin (Cytoskeleton T240), HiLyte Fluor-488-labeled tubulin (Cytoskeleton TL488M-A) ...
-
bioRxiv - Cell Biology 2020Quote: ... reserving a small amount for protein quantification using Precision Red (Cytoskeleton). Prior to measuring GTPase activity ...
-
bioRxiv - Cell Biology 2021Quote: Soluble tubulin polymerization mix containing 50 μM tubulin protein (Cytoskeleton, T240), 1 μM TRITC-labeled tubulin protein (Cytoskeleton ...
-
bioRxiv - Cell Biology 2021Quote: ... 22 μl of 5 mg ml−1 tubulin protein (Cytoskeleton, T240) was mixed with 2 μl of 5 mg ml−1 TRITC-labeled tubulin protein (Cytoskeleton ...
-
bioRxiv - Genetics 2024Quote: ... Protein concentration was determined using APA assay (Cytoskeleton Inc. Denver, CO, USA) and 50 μg total protein were separated on SDS-PAGE followed by western blot ...
-
bioRxiv - Biochemistry 2023Quote: ... and protein concentrations were estimated using the Precision Red reagent (Cytoskeleton Inc). Protein samples were prepared by mixing 1X LDS (Invitrogen) ...
-
bioRxiv - Cell Biology 2021Quote: ... A mix of 20 μM of porcine brain tubulin protein (Cytoskeleton/Tebu-Bio) with 1 mM GMPCPP (Jena Bioscience ...
-
bioRxiv - Biophysics 2020Quote: 0.4 µM Myosin Motor protein S1 Fragment (Rabbit Skeletal Muscle, Cytoskeleton #CS-MYS04) was incubated either alone or with 2 µM UCS proteins for 10 minutes either at 42°C or on ice in Mg-buffer (10mM Hepes-KOH ...
-
Deletion or inhibition of PTPRO mitigates diet-induced hepatic steatosis and inflammation in obesitybioRxiv - Immunology 2022Quote: ... and Tyr-phosphorylated proteins were collected using Anti-phosphotyrosine Affinity Beads (Cytoskeleton, USA). Proteins were concentrated using the TCA/acetone precipitation method (Fic et al. ...
-
bioRxiv - Microbiology 2020Quote: ... recombinant human cofilin-1 (CFL1 P23528) and arp2/3 complex (ARPC4 Q148J6) from bovine brain were all purchased from Cytoskeleton Inc ...
-
bioRxiv - Cell Biology 2021Quote: ... Lysates were then incubated with 30 µg of Ras-GTP Binding Domain (RBD of human c-Raf kinase) fused with GST associated with beads (#RF02, Cytoskeleton) at 4°C for 1h ...
-
Gα13 loss promotes tumor progression in the KPC transgenic mouse model of advanced pancreatic cancerbioRxiv - Cancer Biology 2021Quote: ... and the protein concentration was determined using Precision Red solution (Cytoskeleton, Inc., Denver, CO) according to the manufacturer’s instructions ...
-
bioRxiv - Pathology 2023Quote: ... and the protein concentration was determined using Precision Red solution (Cytoskeleton, Inc., Denver, CO). Equal amounts of protein were separated with a 10% SDS-PAGE electrophoresis gel ...
-
bioRxiv - Cell Biology 2021Quote: Actin Polymerization Biochem Kit (Cytoskeleton) was used to investigate F-actin depolymerization by cofilin in the absence or presence of control protein BSA ...
-
bioRxiv - Plant Biology 2020Quote: ... 13 nM Arp2/3 protein complex and 15 nMWASp-VCA domain-GST purified (Cytoskeleton, USA) along with freshly purified ArF-BAR protein in concentrations of 200 ...
-
bioRxiv - Cancer Biology 2019Quote: ... Equal protein levels were confirmed by a control western blot of rabbit anti-Kinesin (Cytoskeleton) from the cleared lysates ...
-
bioRxiv - Cell Biology 2022Quote: The Microtubule Binding Protein Spin-down assay was performed according to the manufacturers’ protocol (Cytoskeleton). Briefly ...
-
bioRxiv - Cell Biology 2022Quote: ... Pyrene-labeled actin was incubated with recombinant ARP2/3 protein complex alone (Cat# RP01P, Cytoskeleton) or along with the human recombinant proteins ...
-
bioRxiv - Cancer Biology 2023Quote: ... Rabbit polyclonal antibody recognizing the Kif11/Eg5 protein (Cat. #AKIN03, discontinued) was purchased from Cytoskeleton, Inc ...
-
bioRxiv - Biophysics 2022Quote: ... provided along with the kit (Cytoskeleton) was used to detect G- and F-actins ...
-
bioRxiv - Cell Biology 2021Quote: ... was mixed with 2 μl of 5 mg ml−1 TRITC-labeled tubulin protein (Cytoskeleton, TL590M) and 1 μl of 5 mg ml−1 Biotin-XX-labeled tubulin (Pursolutions ...
-
bioRxiv - Cell Biology 2024Quote: ... The protein was then transferred to a clean tube and quantified using PrecisionRed (Cytoskeleton; ADV02-A) at OD600.
-
bioRxiv - Biochemistry 2024Quote: ... The cells were lysed and protein lysates were used for G-LISA analysis per manufacturer’s (Cytoskeleton) instructions ...
-
bioRxiv - Cancer Biology 2021Quote: Rac1 and RhoA GTPase actitation was measured by G-LISA Rac1 activation assay Kit and G-LISA RhoA activation Kit (Cytoskeleton), according to manufacturer’s protocol ...
-
bioRxiv - Neuroscience 2021Quote: ... assays were carried out using kits from Cytoskeleton, Inc ...
-
bioRxiv - Cell Biology 2020Quote: Activated RhoA pulldown kits were purchased from Cytoskeleton. Sample lysates were prepared similar to the protocol used for G-LISAs ...
-
bioRxiv - Physiology 2022Quote: ... the Cdc42 activation kit (Cytoskeleton Inc., Denver, USA) was used according to manufacturer’s instructions ...