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Citations for Cytoskeleton :
251 - 300 of 319 citations for Actin Alpha 2 Smooth Muscle ACTA2 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... Actin (2µM; 7% pyrene-labeled) was then polymerized in the presence of 20nM bovine Arp2/3 complex (Cytoskeleton Inc.) plus MBP-tagged fusion proteins in control buffer supplemented with 0.2mM ATP and 0.5mM DTT ...
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bioRxiv - Cell Biology 2022Quote: ... before incubating for 5 minutes at room temperature in 40 ng/µl rhodamine-labeled actin (Cat# AR05, Cytoskeleton, Inc.) freshly prepared in cytoskeletal buffer ...
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bioRxiv - Biophysics 2021Quote: ... the cells were rinsed thoroughly and stained with 1:200 solution of secondary antibody in PBS for 2 hours followed by staining with Acti-Stain™ 670 Fluorescent Phalloidin (Cytoskeleton.Inc, catalog no. PHDN1). Then the cover-slides were mounted on glass slides using ProLong Diamond Antifade Mountant (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: G- and F-actin were fractionated and enriched using a commercial in vivo assay biochem kit (Cytoskeleton Inc, Colorado, USA), according to the manufacturer’s instructions with minor modifications ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were then washed three times and incubated with SiR-actin (100 nM in PBS; Cytoskeleton; catalog number: CY-SC001) at 4°C until imaging (minimum length of staining ...
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bioRxiv - Developmental Biology 2021Quote: ... cleaved proteins (quantified via a Bradford Assay) were incubated at room temperature for one hour with 0 or 5μM polymerized chicken F-actin (Cytoskeleton, Inc.). BSA was used a negative control ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were then washed three times and incubated with SiR-actin (100 nM in PBS; Cytoskeleton; catalog number: CY-SC001) at 4°C until imaging (minimum length of staining ...
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bioRxiv - Biochemistry 2023Quote: ... 5 mM guanidine carbonate and 1.2 mM ATP (from Actin Polymerization Buffer, cat. BSA02, and ATP, cat. BSA04, Cytoskeleton Inc.). Samples were incubated at 37 °C for 2 h.
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bioRxiv - Cell Biology 2023Quote: ... Samples were then washed three times in PBS and stored in PBS containing SiR-actin (100 nM; Cytoskeleton; CY-SC001) at 4°C until imaging ...
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bioRxiv - Cell Biology 2019Quote: ... The supernatant was carefully removed and the pellet was resuspended in a volume of F-actin depolymerization buffer (FAD-02, Cytoskeleton Inc.) matching the volume of the supernatant ...
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bioRxiv - Neuroscience 2023Quote: ... Quantification of structural change (i.e., neurite shape and growth) was performed using f-actin labeling with rhodamine phalloidin (Cytoskeleton PHDG1-A). All morphometric measures were conducted and quantified using ImageJ (NIH ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were then washed three times in PBS and stored in PBS containing SIR-actin (100 nM; Cytoskeleton; catalogue number: CY-SC001) at 4°C until imaging ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were then washed three times in PBS and stored in PBS containing SiR-actin (100 nM; Cytoskeleton; catalog number: CY-SC001) at 4°C until imaging ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were then washed three times in PBS and stored in PBS containing SiR-actin (100 nM; Cytoskeleton; catalog number: CY-SC001) at 4°C until imaging ...
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bioRxiv - Biophysics 2022Quote: BJ-5ta cells were seeded on RGD-54pN or LDVP-54pN surfaces for 40 min and 20 nM SiR-actin and 10 µM verapamil (Cytoskeleton, CY-SC001) were added 20 min before imaging ...
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bioRxiv - Cell Biology 2021Quote: ... 2 μM unlabeled porcine tubulin (Cytoskeleton) prepared in binding buffer containing 10 mM DTT and 0.2 mM PMSF was added and incubated overnight on a shaker in a cold room ...
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bioRxiv - Cell Biology 2019Quote: ... HCLE cells were pre-loaded with either CellMask™ Deep Red Plasma membrane stain as described above or 1 μM of SiR-Actin Spirochrome probe (Cytoskeleton Inc., Denver, CO) for 10 minutes at 37°C and 5% CO2 ...
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bioRxiv - Cell Biology 2022Quote: ... CT04 (Cytoskeleton; 2 µg/mL; vehicle: dH2O) was added to the media for 2 hours as pre-treatment plus an additional 4 hours with or without Wnt7a.
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bioRxiv - Microbiology 2023Quote: ... IP of total SUMOylated protein fractions used either SUMO-1 or SUMO-2/3 Ab included with the Signal Seeker SUMOylation 1 or 2/3 Detection Kit (Cytoskeleton) and IPs were carried out according to the manufacturer instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... and 2‰ MemGlow 560 (Fluorogenic Membrane Probe, Cytoskeleton), and imaged on the imaging setup described below.
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bioRxiv - Cell Biology 2022Quote: ... 2 µL of 10 mg/mL unlabeled tubulin (Cytoskeleton) was mixed with 2 µL o f 1 mg/mL biotin-tubulin and 2 µL of 1 mg/mL dye-labeled tubulin on ice ...
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bioRxiv - Developmental Biology 2021Quote: ... Membranes were incubated in primary antibody diluted in TBST (1:500 anti-RHOA antibody, Cytoskeleton ARH04) or diluted in block (1:30,000 anti-a-alpha tubulin antibody ...
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bioRxiv - Neuroscience 2023Quote: ... then rinsed and sequentially incubated with primary antibodies to RhoA followed by HRP secondary antibodies (Cytoskeleton), and the colorimetric reaction was performed for 15 min at 37 °C ...
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bioRxiv - Cancer Biology 2020Quote: ... or 2 μg/mL Rho A Inhibitor I (Cytoskeleton, Inc.) conditions and imaged immediately after designated incubation time as per commercial protocol (Cytoskeleton Inc.) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... testes were stained with 2 μM SiR-Actin59 (Cytoskeleton, Inc.) for 2-3 hours at room temperature and incubated with Hoechst 33342 (Invitrogen ...
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bioRxiv - Neuroscience 2022Quote: ... 2 μL of 1 mg/mL biotinylated tubulin (Cytoskeleton Inc., #T333P), and 2 μL of 10 mg/mL unlabeled tubulin (Cytoskeleton Inc. ...
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Formation of a giant unilocular vacuole via macropinocytosis-like process confers anoikis resistancebioRxiv - Cell Biology 2024Quote: ... Rho inhibitor I (Cytoskeleton, CT04-A, used at 2 μg/ml), Y-27632 (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... WT Caco-2 cells were treated with 1ug/ml CN03 (Cytoskeleton, Inc) or 1μg/ml C3 Transferase (Cytoskeleton ...
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bioRxiv - Neuroscience 2022Quote: ... and 2 μL of 10 mg/mL unlabeled tubulin (Cytoskeleton Inc., #T240) was mixed on ice ...
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bioRxiv - Cell Biology 2020Quote: Colchicine-competition assays were performed with 2 μM purified tubulin (Cytoskeleton, Inc) taken from a 10 mg/mL stock prepared in tubulin buffer as above ...
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bioRxiv - Cell Biology 2020Quote: ... Monoclonal Mouse anti-RhoA antibody (Cytoskeleton inc. Cat#ARH05) was used at 1:500 dilution in TBST overnight.
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bioRxiv - Cell Biology 2020Quote: ... Monoclonal Mouse anti-RhoA antibody (Cytoskeleton inc. Cat#ARH05) was obtained through the Cytoskeleton inc Rho A activation Assay Biochem Kit and used at 1:500 ...
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bioRxiv - Immunology 2022Quote: ... Monoclonal Rac1 antibodies (Cat. # ARC03) were purchased from Cytoskeleton.
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bioRxiv - Immunology 2022Quote: Monoclonal Rac1 antibodies (Cat. # ARC03) were purchased from Cytoskeleton.
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bioRxiv - Microbiology 2020Quote: ... The treatment consisted of 2 µg/ml of Rho inhibitor I (Cytoskeleton, CT04) for 2 h or 20 µM of Y27632 (Sigma ...
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bioRxiv - Biophysics 2023Quote: ... cells were incubated 2 hours with C3-transferase (Cytoskeleton Inc., cat. CT03-A) at a concentration of 1μg/mL ...
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bioRxiv - Molecular Biology 2019Quote: Mouse antibodies against Myc-tag (M185-3L/M047-3, MBL, Nagoya, Japan), rabbit antibody against GFP-tag (D110008, BBI, Shanghai, China) and sheep antibody against Tubulin (ATN02, Cytoskeleton, Denver, United States) were subjected to Western blot according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... Monoclonal mouse anti-Cdc42 monoclonal antibody (1:250, Cytoskeleton, Cat. # ACD03), anti-Rac1 (1:250 ...
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bioRxiv - Biochemistry 2020Quote: ... provided as a lyophilized powder and resuspended at 2 mM anhydrous DMSO was purchased from Cytoskeleton Inc ...
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bioRxiv - Cell Biology 2021Quote: ... was mixed with 2 μl of 5 mg ml−1 TRITC-labeled tubulin protein (Cytoskeleton, TL590M) and 1 μl of 5 mg ml−1 Biotin-XX-labeled tubulin (Pursolutions ...
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bioRxiv - Cell Biology 2022Quote: ... Microtubules were made by mixing 1 μl of HiLyte Fluor 488 tubulin (2 mg/ml, Cytoskeleton), 2 μl biotinylated tubulin (2 mg/ml ...
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bioRxiv - Molecular Biology 2023Quote: Microtubules were made by mixing 1.5 μL of 2 mg/mL HiLyte Fluor 488 tubulin (Cytoskeleton), 2 μL of 2 mg/mL ...
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bioRxiv - Cell Biology 2021Quote: ... and incubation in secondary antibody and Acti-Stain 670 Phalloidin (Cytoskeleton, inc), plus Hoechst (Thermo Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... moderate amounts of anti-ac-K antibody agarose beads (Cytoskeleton, Inc., USA) were added to the lysate and incubated for 12 h at 4°C ...
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bioRxiv - Cell Biology 2021Quote: Fluorescent MTs were polymerized at 2 mg/ml by incubating tubulin and rhodamine labeled tubulin (Cytoskeleton Inc.) at 10:1 ratio in BRB80 with 1 mM DTT and 1mM GTP for 5 min on ice ...
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bioRxiv - Cell Biology 2023Quote: ... 2 mM EGTA) supplemented with 4.51% of sucrose to adjust osmotic pressure (Cytoskeleton buffer with sucrose; CBS) and then fixed in 3% PFA in CBS for 15 min ...
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bioRxiv - Cancer Biology 2021Quote: ... Primary antibodies used were as follows: mouse anti-RAC1 (Cytoskeleton #ARC03, 1:250); mouse anti-RHOA (Cytoskeleton #ARH05 ...
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bioRxiv - Cell Biology 2021Quote: ... The following primary antibodies were used: rabbit anti-cofilin (Cytoskeleton ACFL02; 1:500), rabbit anti-Ser3-phosphorylated cofilin (Cell Signaling Technology #3311 ...
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bioRxiv - Cancer Biology 2023Quote: ... The primary antibodies used were mouse anti-Rac1 (1:500, Cytoskeleton Inc, #ARC03) and rabbit anti-GAPDH (1:3000 ...
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bioRxiv - Microbiology 2023Quote: ... the samples were washed 2× with IF buffer and incubated with rhodamine phalloidin (PHDR1) (1:500) (Cytoskeleton, Inc.) for 30 minutes in the dark at 4 °C ...