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Citations for Cytoskeleton :
151 - 200 of 319 citations for Actin Alpha 2 Smooth Muscle ACTA2 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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The T-cell niche tunes immune function through modulation of the cytoskeleton and TCR-antigen forcesbioRxiv - Bioengineering 2024Quote: ... and SiR-Actin (Cytoskeleton, Inc # CY-SC001). Samples were run on Amnis ImagestreamX MK II at 60x in EDF mode and data was analyzed in IDEAS software.
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bioRxiv - Cell Biology 2021Quote: The pyrene-actin polymerization and depolymerization assays were performed using the Actin Polymerization Biochem Kit (Cytoskeleton, Inc., Denver, CO) following the manufacturer protocol ...
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bioRxiv - Biophysics 2020Quote: ... 10% rhodamine-labeled F-actin was prepared by co-polymerizing 0.9 μM unlabeled actin monomers and 0.1 μM rhodamine-labeled actin monomers (Cytoskeleton, AR05) in the presence of G-Mg (2 mM Tris-HCl pH 8.0 ...
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bioRxiv - Plant Biology 2021Quote: F-actin disruption assays were carried out using the Actin Binding Protein Spin-Down Assay Biochem kit (Cytoskeleton, USA) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: Actin protein levels were calculated using a G-actin/F-actin in vivo Assay Biochem Kit as per manufacturer’s instructions (Cytoskeleton). Briefly ...
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bioRxiv - Neuroscience 2022Quote: ... Day 8 iTF-Microglia were stained for F-actin using 25 nM SiR-actin (Cytoskeleton, Inc; Cat. No. CY-SC001) probe ...
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Rab35 Governs Apicobasal Polarity Through Regulation of Actin Dynamics During Sprouting AngiogenesisbioRxiv - Developmental Biology 2022Quote: Globular and filamentous actin ratios were determined by western blot as described by commercially available G-actin/ F-actin In Vivo Assay Kit (Cytoskeleton). Globular and filamentous immunocytochemistry was performed as previously described [53] ...
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bioRxiv - Cell Biology 2021Quote: ... actomyosin contractility of MECs stably expressing an inducible ROCK construct was induced overnight using 4-hydroxytamoxifen and the actin cortex of these cells was labelled with SiR-actin dye (Cytoskeleton) according to manufacturer’s instructions prior to laser ablation.
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bioRxiv - Cancer Biology 2021Quote: ... F-actin and G-actin protein samples were collected according to the manufacturer’s instructions (#BK037, Cytoskeleton Inc., Denver, CO, USA). F-actin and G-actin were quantified by SDS-PAGE and western blot using actin antibody (#BK037 ...
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bioRxiv - Cell Biology 2022Quote: ... cells were washed with 1xPBS and lysed in appropriate volume of lysis and F-actin stabilization buffer supplemented with 1mM ATP and protease inhibitor mixture for F-actin stabilization (Cytoskeleton). Lysates were centrifuged at 100 000 x g at 37°C for 1 h to separate the G-actin fraction in the supernatants and pelleted F- actin ...
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bioRxiv - Biochemistry 2023Quote: ... was added to either supplemented actin buffer alone or in the presence of 0.4 mg mL-1 G-actin (cat. AKL99, Cytoskeleton Inc.). The final ligand concentration was 2.5 μM ...
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bioRxiv - Biophysics 2021Quote: ... pH 7.5 (actin polymerization buffer, Cytoskeleton, Inc. BSA02) for an hour ...
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bioRxiv - Neuroscience 2019Quote: ... and 5 μM β-actin (human platelets; Cytoskeleton) whose pH was adjusted to 7.25 with KOH ...
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bioRxiv - Neuroscience 2020Quote: ... Supernatants were incubated with biotinylated-actin (Cytoskeleton, Inc), previously prepared as indicated by the manufacturer’s ...
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bioRxiv - Neuroscience 2019Quote: ... Actin-stain 555 Fluorescent Phalloidin was from Cytoskeleton. The siRNA against CaMKIIγ was from GenePharma (Shanghai ...
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bioRxiv - Cell Biology 2019Quote: ... with 8.5% biotin-G-actin (AB07; Cytoskeleton Inc.) and 8.5% Rhodamine-G-actin (APHR ...
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bioRxiv - Cell Biology 2019Quote: ... and 8.5% Rhodamine-G-actin (APHR; Cytoskeleton Inc.) in the presence of F-actin buffer (20 mM Hepes pH 7.4 ...
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bioRxiv - Developmental Biology 2023Quote: ... SiR-actin (100 nM; Cytoskeleton Inc. CY-SC001) was added to hiPSCs in hydrogels for 12 hr following manufacturer’s protocol ...
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bioRxiv - Biophysics 2023Quote: ... 0.3 nM TMR-phalloidin-labeled biotinylated actin (Cytoskeleton) filaments ...
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bioRxiv - Cell Biology 2023Quote: NBD-actin (ATP bound) was purchased from Cytoskeleton. To prepare ADP-actin ...
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bioRxiv - Genetics 2023Quote: ... and SiR-actin probe (1:1000, Cytoskeleton, SC001) in PBS+ for 1 hour at room temperature ...
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The T-cell niche tunes immune function through modulation of the cytoskeleton and TCR-antigen forcesbioRxiv - Bioengineering 2024Quote: ... SiR-Actin (# CY-SC001) was purchased from Cytoskeleton, Inc ...
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bioRxiv - Developmental Biology 2024Quote: ... sir-actin (1/2000, Cytoskeleton, n°CY-SC001) and DAPI for 1h at RT ...
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bioRxiv - Bioengineering 2023Quote: ... Lipids accumulated in HLOs and cytoskeleton were stained with 2 μM BODIPY® 493/503 and 1 μM SiR-Actin (Cytoskeleton Inc., CO, USA), respectively ...
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bioRxiv - Neuroscience 2019Quote: G/F-actin assay was performed according to the manufacturer’s instructions (G/F-actin In Vivo Assay Kit, Cytoskeleton, Denver, CO). G-and F-actin bands on western blots were scanned by densitometry and the ratios of free G-actin to F-actin were calculated.
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bioRxiv - Neuroscience 2021Quote: Hippocampal and cortical tissue from WT and HTZ mice were lysed and homogenized in conditions that stabilize filamentous (F-) and monomeric (G-) actin using an F/G actin commercial assay (Cytoskeleton Inc.). Lysed extracts were ultracentrifuged at 100,000 g for 1 h at 37 °C in order to separate pellets (F-actin ...
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bioRxiv - Microbiology 2022Quote: ... 110 μl of LAS2 buffer was prepared according to the protocol of a G-actin/F-actin In Vivo Assay Biochem Kit (Cytoskeleton, Inc.) and mixed with the pellet ...
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bioRxiv - Cell Biology 2019Quote: ... Increase in fluorescence of pyrene-labeled actin (Cytoskeleton, USA) was measured with excitation at 366 nm and emission at 407 nm.
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bioRxiv - Microbiology 2019Quote: ... 1 μM SiR-actin (CY-SC001, Cytoskeleton, Denver, CO) was added to SCG cultures one hour prior to fixation ...
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bioRxiv - Cell Biology 2021Quote: ... cells were pre-incubated with 50nM siR-actin (Cytoskeleton) for 1hr prior to trypsinization and immediate transfer to an imaging chamber ...
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bioRxiv - Developmental Biology 2022Quote: ... Then 0.5µl of SiR-actin (Cytoskeleton Inc. Denver, CO) (1mM ...
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bioRxiv - Biophysics 2022Quote: ... 20 μg aliquots of lyophilized rhodamine actin (Cytoskeleton AR05) were resuspended in 18 μL of G-Ca buffer and 2 μL of milli-Q water ...
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bioRxiv - Cell Biology 2021Quote: ... with or without 1µM SiR-Actin (CY-SC001, Cytoskeleton) or 2 nM Calyculin A (101932-71-2 ...
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bioRxiv - Developmental Biology 2021Quote: ... Actin-stain 670 phalloidin (1:140, Cytoskeleton, PHDN1-A), DAPI (5 μM).
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bioRxiv - Cancer Biology 2021Quote: ... propidium iodide and 500 nM SiR-Actin (Cytoskeleton, Inc) for 6 h and imaged again (144 h timepoint) ...
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bioRxiv - Cell Biology 2022Quote: Cell tracing dyes including SPY555-Actin (Cytoskeleton CY-SC202), SPY650-Tubulin (Cytoskeleton CY-SC503 ...
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Membrane-dependent actin polymerization mediated by the Legionella pneumophila effector protein MavHbioRxiv - Molecular Biology 2023Quote: ... Actin polymerization biochem kit (BK003) was purchased from Cytoskeleton Inc ...
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bioRxiv - Cell Biology 2023Quote: ... cells were overlaid with SIR-Actin (Cytoskeleton, CY-SC001) at a final concentration of 1 µM in FluoroBrite DMEM containing 10% FBS and incubated for 1 h ...
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bioRxiv - Developmental Biology 2023Quote: ... ovaries were respectively incubated with SiR-actin (Cytoskeleton TM) at 1:150 and with Wheat Germ Agglutinin (WGA ...
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bioRxiv - Cell Biology 2023Quote: ... F-Actin was stained using Actistain (1:150, Cytoskeleton) for 1h at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... The samples were resolved on 12% SDS-PAGE and detected via Western blot analysis using actin (rabbit anti-actin, BK037 Cytoskeleton Inc.; 1:500) and visualized by ECL (Pierce ...
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bioRxiv - Biochemistry 2021Quote: ... F-actin was labeled with rhodamine-phalloidin (Cytoskeleton, Inc. PHDR1) for 20 min and followed by 3x PBS wash ...
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bioRxiv - Molecular Biology 2020Quote: ... Actin filaments were stained using Phalloidin-Alexafluor 555 (Cytoskeleton Inc) and nuclei with DAPI ...
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bioRxiv - Cell Biology 2021Quote: ... Tissue sections were stained for F-actin by phalloidin (Cytoskeleton), ezrin (Santa Cruz Biotechnology) ...
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bioRxiv - Physiology 2020Quote: ... and 1:1,000 diluted SiR-Actin (Cytoskeleton, Inc.; CY-SC001) in PBST at 4°C overnight ...
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bioRxiv - Biophysics 2023Quote: ... all experimental protocols were identical except 10% biotin-actin (Cytoskeleton) was added to 2 μM G-actin and stabilized with tetramethylrhodamine isothiocyanate-labeled phalloidin in KMg25 buffer (60 mM MOPS pH 7.0 ...
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Trypanosoma brucei colonises the tsetse gut via an immature peritrophic matrix in the proventriculusbioRxiv - Microbiology 2019Quote: ... stained with SiR-actin (1:1000 dilution in PBS, Cytoskeleton Inc.) for 4 hours ...
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bioRxiv - Molecular Biology 2020Quote: ... and actin fibers were stained with Acti-stain 555 (Cytoskeleton Inc). Images were obtained in a Nikon A1R confocal microscope ...
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bioRxiv - Molecular Biology 2022Quote: ... and F-actin with Acti-stain 670 phalloidin (#PHDN1-A, Cytoskeleton).
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bioRxiv - Biochemistry 2023Quote: ... 0.2 mM CaCl2 (from General Actin Buffer, cat. BSA01, Cytoskeleton Inc.), 50 mM KCl ...