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Citations for Jackson ImmunoResearch :
3851 - 3900 of 4145 citations for 7 chloro 5 methylpyrazolo 1 5 A pyrimidine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... This was followed by further rinses in 0.01 M PBS (3 × 10min) and a 12h incubation at 4 °C in biotin-streptavidin conjugated AffiniPure IgG (anti-rabbit-streptavidin Jackson Immunoresearch, 1:200), followed by three washes with 0.01 M PBS ...
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bioRxiv - Immunology 2024Quote: ... washed and overlaid with HRP-conjugated goat anti-mouse secondary antibodies diluted 1:5,000 in blocking solution for 1h at RT (Jackson ImmunoResearch, #115-035-003) and subsequently washed ...
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bioRxiv - Pathology 2023Quote: ... sections were washed with 1X TBS and stained with Alexa Fluor 594 conjugated donkey anti mouse secondary antibody (1:1000, Jackson Immunoresearch, RRID: AB_2340857) and Hoechst 33342 (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... or Alexa 647-labeled species-specific secondary antibodies for 2 hr at a dilution of 1:200 (Invitrogen; Jackson ImmunoResearch, West Grove, PA). Sections were then mounted on slides (ProLong Gold ...
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bioRxiv - Immunology 2022Quote: ... Purified Anti-Mouse CD16/CD32 (Fc Shield) (Clone:2.4G2)) and Goat Anti-Armenian Hamster IgG H+L (1:50; Jackson ImmunoResearch: 127-005-099) in RPMI 10% FCS for 15 minutes at 4°C ...
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bioRxiv - Immunology 2022Quote: ... Purified Anti-Mouse CD16/CD32 (Fc Shield) (Clone:2.4G2)) and Goat Anti-Armenian Hamster IgG H+L (1:50; Jackson ImmunoResearch: 127-005-099) for 15 minutes at 4°C ...
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bioRxiv - Immunology 2022Quote: ... Purified Anti-Mouse CD16/CD32 (Fc Shield) (Clone:2.4G2)) and Goat Anti-Armenian Hamster IgG H+L (1:50; Jackson ImmunoResearch: 127-005-099) for 15 minutes at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... Membranes were subsequently washed with PBS-T and incubated with horseradish peroxidase (HRP)-conjugated secondary antibody goat anti-mouse-IgG (115-035-146, Jackson ImmunoResearch, 1:2,000) for 1 h at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... membranes were incubated with an anti-rabbit IgG horseradish peroxidase-conjugated (HPR) secondary antibody (1:10,000) (cat #211032171, Jackson ImmunoResearch, West Grove, PA) and an anti-mouse IRDye 800CW (1:10,000 ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibody incubation was followed by incubation for 2 h at room temperature with PBS-T containing the following secondary antibodies: AlexaFluor-488-conjugated donkey anti-rabbit (1:1000, Jackson ImmunoResearch Laboratories, 711545152), AlexaFluor-594-conjugated donkey anti-rabbit (1:1000 ...
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bioRxiv - Immunology 2023Quote: ... for 10 minutes and probed for 1 hour at room temperature with the horseradish peroxidase-conjugated mouse anti-rabbit IgG monoclonal antibody at a 1:5,000 dilution (Jackson ImmunoResearch, 211-035-109). Membranes were washed three more times in TBST before protein expression was detected using the Pierce ECL Plus Substrate (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were washed three times in PBS for 10 min each and then incubated in donkey anti-rabbit Alexa 488 and donkey anti-mouse Alexa 647 (Jackson ImmunoResearch, 1:1000) for 2 h at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... molecular weight bands were visualized by peroxidase-conjugated anti-mouse or anti-rabbit secondary antibody (1:5000; Jackson ImmunoResearch Laboratories, West Grove, PA). ECL-plus (Western Lighting ...
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bioRxiv - Bioengineering 2023Quote: ... The plates were washed four times and then incubated with Peroxidase AffiniPure Goat Anti-Human IgG (H+L) (Jackson ImmunoResearch, 1:10,000 dilution) at room temperature for 1 hour ...
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bioRxiv - Neuroscience 2023Quote: ... Ximbio #155259) and the secondary antibody set included either AlexaFluor 594-conjugated donkey anti-chicken IgY (1:500; Jackson ImmunoResearch #703-585-155) or AlexaFluor 546-conjugated goat anti-chicken IgY (1:1000 ...
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bioRxiv - Neuroscience 2023Quote: ... all membranes were incubated with HRP-conjugated goat anti-mouse or anti-rabbit secondary antibody (Jackson ImmunoResearch, West Grove, PA; 1:2000 dilution) corresponding to the primary antibody and developed with the enhanced chemiluminescence method ...
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bioRxiv - Neuroscience 2023Quote: ... slices were incubated for 2 hours with a secondary anti-rabbit antibody conjugated to Alexa 647 (1:500; Jackson Immunoresearch, 111-605-045) with 5% normal goat serum in PBS + 0.3% Tween-20.
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bioRxiv - Neuroscience 2023Quote: ... and then incubated in donkey anti-mouse Cy3 secondary antibody (1:200 in PBS, 715-165-020, RRID: AB_2340811; Jackson ImmunoResearch, West Grove, PA) for 1 hr ...
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bioRxiv - Neuroscience 2023Quote: ... and then probed for 2 hours with goat anti-HRP conjugated Alexa Fluor 647 (1:1000, or 0.5 mg/mL, Jackson ImmunoResearch, West Grove, PA). The anti-HRP antibody recognizes a glycoprotein epitope that is present throughout the fruit fly nervous system ...
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated with a mix of secondary antibodies (1:200 each; Alexa Fluor 488-conjugated goat anti-guinea pig, Molecular Probes; Rhodamine Red™-X-conjugated donkey anti-rabbit, Jackson ImmunoResearch) diluted in 0.05 M TBS ...
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bioRxiv - Immunology 2023Quote: ... clone CMYC-45F) to label for RBD expression and 1:200 PE-conjugated goat anti-human-IgG (Jackson ImmunoResearch, cat. 109-115-098) to label for bound mAb.
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bioRxiv - Neuroscience 2023Quote: ... Sections were then rinsed in 0.1M PBS and incubated in donkey anti-rabbit horseradish peroxidase-conjugated secondary antibody (1:500, catalog #711-036-152, Jackson ImmunoResearch; RRID: AB_2340590) for 1hr ...
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bioRxiv - Pathology 2023Quote: ... Alexa Fluor 594 AffiniPure F(ab’)[Fragment Goat Anti-Rabbit IgG (H+L) (1:200, 111-586-144, Jackson ImmunoResearch, West Groove, PA), Alexa Fluor 488 AffiniPure F(ab’)[Fragment Donkey Anti-Goat IgG (H+L ...
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bioRxiv - Neuroscience 2023Quote: ... Mouse-on-mouse interference was reduced using Affinpure donkey polyclonal anti-mouse IgG Fab fragments at a 1:20 concentration (715-007-003; Jackson ImmunoResearch Laboratories Inc) for two hours preceding incubation with the unconjugated primary antibodies targeting dsRNA and SARS-CoV-2 spike as described above.
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bioRxiv - Cancer Biology 2023Quote: ... the membranes were incubated with alkaline phosphatase-conjugated goat anti-rabbit IgG (1:10000) (Catalog: 111-055- 144, RRID: AB_2337953, Jackson ImmunoResearch West Grove, PA) and stained using 1-Step NBT/BCIP solution (ThermoFisher).
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bioRxiv - Developmental Biology 2023Quote: ... Cells were washed 3x with 1 mL of FACS buffer before 30 minutes of staining in FACS buffer with a 1:1000 dilution of anti-mouse IgM Alexaflour-488 secondary antibody (Jackson Immunoresearch, Cat#715545140) on ice ...
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bioRxiv - Bioengineering 2023Quote: ... The beads were then incubated with a 1:300 dilution of goat anti-human Fc Alexa Fluor 647 (Jackson ImmunoResearch 109-605-098) in PBSB on ice for 4 min ...
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bioRxiv - Bioengineering 2023Quote: ... The beads were then incubated with a 1:300 dilution of goat anti-human Fc Alexa Fluor 647 (Jackson ImmunoResearch, 109-605-098) in PBSB on ice for 4 min ...
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bioRxiv - Immunology 2023Quote: ... The CD40 binding signal was detected by adding a 1:5,000 dilution of goat anti-human Fab/F(ab’)2 IgG secondary-HRP antibody (Jackson ImmunoResearch, 109-035-006) or a 1:20,000 dilution of goat anti-human Fc IgG secondary-HRP antibody (Jackson ImmunoResearch ...
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bioRxiv - Immunology 2023Quote: ... and the presence of IgG was detected using anti-mouse IgG-specific horseradish peroxidase (HRP; 1:1500; Jackson ImmunoResearch, West Grove, PA, USA). The presence of rabbit anti-mouse IgG-specific HRP was visualized using trimethylboron substrate and measured using absorbance at 450 nm with a reference wavelength of 570 nm ...
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bioRxiv - Microbiology 2023Quote: ... the membranes were incubated with horseradish peroxidase (HRP)-conjugated secondary antibodies: donkey anti-rabbit IgG-HRP (Jackson ImmunoResearch, 711-035-152; 1:5,000); donkey anti-mouse IgG-HRP (Jackson ImmunoResearch ...
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bioRxiv - Neuroscience 2023Quote: ... Secondary antibodies were made in donkey or goat and conjugated with: A488 (anti-chicken, 1:500, Jackson Immunoresearch, Ely, UK, 703-545-155); A488 (anti-goat ...
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bioRxiv - Immunology 2023Quote: ... Membranes were washed three times for 10 min and incubated with a 1:10,000 dilution of horseradish peroxidase-conjugated anti-rabbit antibodies (Jackson ImmunoResearch, West Grove, PA) at room temperature for 2 hrs ...
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bioRxiv - Cell Biology 2023Quote: ... The membrane was washed 3X 5 minutes in TBS + 0.1% Tween and then incubated for 1 hour in milk containing donkey-anti-mouse IgG secondary antibodies conjugated with HRP (Jackson ImmunoResearch Laboratories, Inc.) diluted at 1:5000 in block ...
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bioRxiv - Neuroscience 2023Quote: ... 1:75,000) against human kisspeptin-54 (aa 68-121 of NP_002247.3) (9, 53) using biotinylated secondary antibodies (Jackson ImmunoResearch Europe, Cambridgeshire, UK; 1:500), the ABC Elite reagent (Vector ...
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bioRxiv - Neuroscience 2023Quote: ... The following secondary antibodies were used at a 1:1,000 dilution for staining: Alexa Fluor 647 AffiniPure donkey anti-rabbit IgG (H+L) (Jackson ImmunoResearch, 711-605-152), Alexa Fluor 647 AffiniPure donkey anti-guinea pig IgG (H+L ...
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bioRxiv - Neuroscience 2023Quote: ... pH 7.4) and incubated with a biotinylated goat anti-rat polyclonal secondary antibody (1:300; Jackson ImmunoResearch, Baltimore, USA, cat# 112-065-167) in TBS containing 0.05% Triton X-100 for 90 min at RT ...
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bioRxiv - Biophysics 2023Quote: ... Membranes were then washed with TBST (3 times for 15 min) and incubated with anti-rabbit-HRP (111-036-045; Jackson ImmunoResearch; 1:5000) secondary antibody ...
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bioRxiv - Neuroscience 2023Quote: ... sections were washed 3 x 15 min with 0.1 M PBS prior to a 2-hour incubation in secondary antibodies FITC (1:50 in 0.1 M PBS, Jackson ImmunoResearch, 703-095-155), Cy3 (1:50 in antibody diluent ...
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bioRxiv - Neuroscience 2023Quote: ... followed by tyramine signal amplification and treatment with an Alexa Fluor 647-conjugated streptavidin antibody (1:200; Jackson ImmunoResearch Laboratories; RRID: AB_ 2341101). After rinsing with three PBS exchanges (10 min each) ...
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bioRxiv - Genomics 2023Quote: ... 3 times at room temperature and then incubated with secondary antibodies: Cy3 donkey anti-goat (Jackson ImmunoResearch, Cat# 715-165-150, 1:500), Alexa Fluor 488 donkey anti-mouse (Jackson ImmunoResearch ...
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bioRxiv - Neuroscience 2023Quote: ... 019-19741) and secondary Biotin-SP-conjugated AffiniPure Donkey Anti-Rabbit IgG (H+L) at 1:250 (Jackson ImmunoResearch cat: 711-065-152). For oligodendrocytes ...
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bioRxiv - Neuroscience 2024Quote: ... The wells were probed with horseradish peroxidase (HRP)-conjugated secondary antibody [goat anti-mouse-IgG (Jackson ImmunoResearch, catalog #115-005-003; 1:4000)] for 1 hr ...
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bioRxiv - Neuroscience 2024Quote: ... the tissue was washed 3 times for 5 min each with 1X PBS + 0.2% Triton-X and then incubated overnight in blocking buffer containing Alexa-Fluor-conjugated secondary antibodies (Jackson ImmunoResearch Laboratories, 1:500). Upon completion of incubation with the secondary antibodies ...
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bioRxiv - Microbiology 2024Quote: ... membranes were washed 3 times with PBS-T before incubation with HRP-conjugated secondary antibodies (Jackson ImmunoResearch Laboratories, 115-035-146; 1:5000) for 1 h at room temperature ...
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bioRxiv - Molecular Biology 2024Quote: ... sections were incubated with biotin-SP-conjugated goat anti-mouse IgG1 (1:1,000) (catalog #: 111-065-003; Jackson ImmunoResearch; West Grove, PA, USA) in 2.5% BSA for 90 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... Coverslips were then incubated with primary antibodies diluted in the antibody diluent as following: mouse anti-biotin (Jackson ImmunoResearch, #200-002-211, 1:2,000), rabbit anti-biotin (Bethyl Laboratories ...
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bioRxiv - Developmental Biology 2021Quote: ... The secondary antibodies were Alexa Fluor® 488 conjugated anti-rabbit and Alexa Fluor® 488 conjugated anti-mouse (Jackson ImmunoResearch, 1:200 diluted). After staining ...
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bioRxiv - Developmental Biology 2020Quote: ... the embryos were incubated overnight at 4 °C in Cy3-conjugated goat anti-rabbit antibody (1:500 in BB; Jackson Immunoresearch, 111-165-144), Alexa488-conjugated goat anti-mouse (1:500 in BB ...
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bioRxiv - Molecular Biology 2020Quote: ... Primary antibody against DPF-3 was used at 1:200 dilution and anti-rat secondary antibodies fused to HRP (Jackson ImmunoResearch, 712-035-150) were used at 1:2000 dilution ...