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Citations for Jackson ImmunoResearch :
3601 - 3650 of 4145 citations for 7 chloro 5 methylpyrazolo 1 5 A pyrimidine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... containing 1:1000 dilutions of the goat anti-Rabbit Alexa 488 and goat anti-Rat Alexa 594 antibodies or a 1:400 dilution of the donkey anti-mouse Alexa 488 or of the donkey anti-mouse Alexa 594 (Jackson ImmunoResearch), together with Hoechst staining (30 min ...
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bioRxiv - Neuroscience 2022Quote: ... retinas were incubated for 3 hours at room temperature with Alexa Fluor-conjugated secondary antibodies (1:1,000; Thermo Fisher Scientific or Jackson ImmunoResearch). Whole retinas were flattened with photoreceptor side down onto nitrocellulose filters ...
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bioRxiv - Neuroscience 2023Quote: ... brain slices were incubated with a secondary antibody (Secondary antibody for c-Fos staining, Cy3-Goat anti-Guinea pig, 1:500 dilution, Jackson ImmunoResearch, Cat ...
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bioRxiv - Neuroscience 2023Quote: ... Highly cross-adsorbed Alexa dye-conjugated secondary antibodies generated in donkey toward the appropriate species (1:500; Alexa488, Alexa594, and Alexa647; Jackson ImmunoResearch) were used as detection antibodies ...
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bioRxiv - Molecular Biology 2022Quote: ... which was detected by incubation with rhodamine-tagged anti-rabbit IgG antibody (red, 1:200) (Jackson ImmunoResearch; Cat:111-025-003) for 1 h at RT ...
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bioRxiv - Plant Biology 2023Quote: ... Chemiluminescent detection was performed after incubation with a 1/10000 dilution of Peroxidase AffiniPure goat anti-rabbit or anti-mouse IgG (H+L; Jackson ImmunoResearch) in 1 % non-fat milk in TBS for 1 h at room temperature ...
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bioRxiv - Developmental Biology 2023Quote: ... double WMIF was performed as described in (Soh et al, 2020) with goat α-rabbit-horseradish peroxidase (1:500, AB_2307391, Jackson ImmunoResearch) and TSA fluorescein detection of pSmad2 activity (Soh et al ...
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bioRxiv - Cancer Biology 2023Quote: ... and the membrane was washed with 0.1%TBS-T and was incubated for 2hr at room temperature with respective secondary anti-mouse or anti-rabbit antibody conjugated with horseradish peroxidase (HRP) (1:5000, Jackson ImmunoResearch Laboratories ...
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bioRxiv - Cancer Biology 2023Quote: ... B cells were plated at a concentration of 5L×L105 cells per 200LμL in round-bottom 96-well plates treated with 0.1% B16F10 melanoma cell lysis or 10 µg/mL goat anti-mouse IgM F(ab’)2 (115-006-020, Jackson ImmunoResearch). B cells were treated with NR-V04 or DMSO for 24 hours and collected for flow cytometry analysis.
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bioRxiv - Cell Biology 2023Quote: ... Immunofluorescence visualization was performed with Alexa 488 or 590 or 647-conjugated secondary antibodies (1:500 dilution; Cat. Nos. 112545143; 111585144; 111605144, Jackson ImmunoResearch). Sections were counterstained with Hoechst 33342 (2□μg/mL dilution ...
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bioRxiv - Genetics 2023Quote: ... the brains are incubated with secondary antibodies 1:500 Donkey anti-Rat fluorophore 647 (Jackson ImmunoResearch Laboratories Inc., 712-605-153) and 1:1000 DAPI for one hour at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... slides were washed in 0.01M PBS and incubated in the same blocking buffer but with appropriate secondary antibodies (647 donkey anti-guinea pig, 1:400, 488 donkey anti-mouse and 594 donkey-anti rabbit, Jackson Immunoresearch). The slides were then rinsed in 0.01M PBS and cover slipped using Fluro media with DAPI to stain nuclei ...
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bioRxiv - Molecular Biology 2023Quote: ... Second antibodies used were the Alexa Fluor 488 donkey anti-Rabbit IgG (H+L) (Jackson ImmunoResearch, 711-545-152, 1:1000). Nuclei were stained with Hoechst33342 (Sigma-Aldrich ...
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bioRxiv - Immunology 2023Quote: ... sections were incubated with one or several of the following cross-adsorbed secondary antibodies at 1:250: Goat anti-rabbit-Alexa647 (Jackson ImmunoResearch), Goat anti-mouse-Alexa647 (Jackson ImmunoResearch) ...
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bioRxiv - Developmental Biology 2023Quote: ... The secondary antibodies were Fluorescein (FITC) AffiniPure donkey anti-rabbit IgG (1:200, 711-096-152, Jackson Immunoresearch Laboratories, PA, USA) and Cy5 AffiniPure donkey anti-mouse IgG (1:200 ...
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bioRxiv - Pathology 2023Quote: ... the slides were incubated with anti-CD31 antibody (rat) for 32 minutes at 37 °C followed by rabbit anti-rat (1:500; Jackson ImmunoResearch) and detected using the OmniMap system (Ventana ...
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bioRxiv - Molecular Biology 2023Quote: ... slides were incubated for 90 minutes in secondary 1:1000 biotin solution (anti-mouse IgG1, Jackson ImmunoResearch; West Grove, PA, USA), followed by a 60-minute incubation with secondary 1:500 streptavidin (SA-HRP ...
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bioRxiv - Immunology 2023Quote: ... incubated for 1h at RT with HRP-conjugated goat anti-mouse secondary antibodies (115-035-003, dilution 1:5000, Jackson ImmunoResearch) or anti-rabbit (111-035-003 ...
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bioRxiv - Neuroscience 2023Quote: ... we incubated sections for 2 hours in a PBT-NDS-azide solution containing a 1:500 dilution of either donkey anti-rabbit (#711-065-152) or donkey anti-rat (#712-065-153; Jackson ImmunoResearch) secondary antibody ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then washed 3 times in PBS and immediately transferred to the secondary antibody solution: Cy3 goat anti-mouse (1:500, Jackson ImmunoResearch) for 2 hours at home temperature ...
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bioRxiv - Immunology 2023Quote: ... Indo bound/Indo free ratio baseline was recorded for 1 min before addition of pre-warmed anti-mouse IgM F(ab’)2 (Jackson ImmunoResearch) to a final concentration of 2 or 10 μg/ml ...
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bioRxiv - Neuroscience 2023Quote: ... then blocked for 45 min before 2 h incubation at room temperature in Alexa Fluor 488-conjugated donkey anti-chicken IgG (1:400 in blocking solution, Jackson ImmunoResearch). Sections were rinsed in PBS after the final incubation and mounted with Vectashield Hardset Mounting Medium with DAPI (Vector Labs) ...
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bioRxiv - Cell Biology 2022Quote: ... Primary antibodies were washed with PBS1x and then detected using donkey anti-rabbit or anti-mouse FITC or Cy3 (1:500) coupled secondary antibodies (Jackson ImmunoResearch). The sections were washed with PBS1x and mounted ...
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bioRxiv - Cell Biology 2023Quote: ... The membranes were washed for 30 min and subsequently incubated with secondary antibody HRP-Goat anti-rabbit IgG (polyclonal, 1:5000 dilutions, Jackson ImmunoResearch) for 2 h at RT ...
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bioRxiv - Cell Biology 2022Quote: ... sections were blocked for 1 hour at room temperature in blocking buffer: 10% normal donkey serum (Jackson ImmunoResearch, 017-000-121) in PBST (0.1% TritonX-100 in PBS) ...
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bioRxiv - Immunology 2023Quote: ... incubated for 1h at RT with HRP-conjugated goat anti-mouse secondary antibodies (115-035-003, dilution 1:5000, Jackson ImmunoResearch) or anti-rabbit (111-035-003 ...
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bioRxiv - Immunology 2023Quote: ... the membranes were washed for 30 min and subsequently incubated in secondary antibody HRP-Goat anti-rabbit IgG (polyclonal, 1:5000 dilution, Jackson Immunoresearch) for 2h at RT ...
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bioRxiv - Neuroscience 2023Quote: ... larvae were incubated in the dark at 4°C for 24 h with secondary antibodies (1:500, Alexa Fluors 488, 555, 594 and 647, Jackson Immunoresearch). For CASK immunostaining experiments larvae were incubated in Hoechst stain (1:8000 ...
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bioRxiv - Physiology 2023Quote: ... membranes were washed with TBS-tween and then probed for 1 hour at room temperature with anti-mouse or anti-rabbit secondary antibodies (Jackson ImmunoResearch, West Grove ...
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bioRxiv - Neuroscience 2022Quote: ... Secondary antibodies used include: Donkey secondary antibodies conjugated to Alexa Fluor 488/647 or Rhodamine Red-X (1:250, Jackson ImmunoResearch), horse anti-mouse DyLight 649 (1:250 ...
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bioRxiv - Neuroscience 2023Quote: ... the sections were incubated for 1 h with a biotinylated donkey antibody to guinea pig IgG (10 μg/ml; 706-065-148, Jackson ImmunoResearch). The sections were further incubated with Alexa594-conjugated streptavidin (2.5 μg/ml).
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bioRxiv - Neuroscience 2023Quote: ... floating sections were blocked for 1 hour at room temperature in blocking solution (3% normal goat serum (NGS, Jackson ImmunoResearch Laboratories), 0.3% Triton X-100 (Fisher) ...
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bioRxiv - Physiology 2023Quote: ... sections were incubated for 90 min in secondary biotin solution (anti-mouse IgG1, 1:1000, Jackson ImmunoResearch; West Grove, PA, USA), followed by a 60-min incubation with a secondary cocktail containing AF647 anti-mouse IgG2b (1:200 ...
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bioRxiv - Neuroscience 2023Quote: ... Brains were then incubated in a block solution containing a donkey anti-rat or donkey anti-mouse secondary antibody conjugated with Cy5 or Cy3 (1:400 dilution, Jackson ImmunoResearch) for 2 hours at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... was used followed by a donkey anti-chicken secondary antibody conjugated to AF488 (1:500; AB_2340375, Jackson Immunoresearch, West Grove, USA).
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bioRxiv - Neuroscience 2023Quote: ... the cerebellar slices were incubated in an HRP-conjugated anti-rabbit IgG (1:100, 111-035-144; Jackson ImmunoResearch, Pennsylvania, USA) for 1 hour at RT and washed with PBS three times for 3 minutes each at RT ...
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bioRxiv - Neuroscience 2023Quote: ... the membranes were washed with TBST and incubated with Peroxidase AffiniPure anti-rabbit and mouse IgG antibodies (1:10,000, Jackson ImmunoResearch, USA) for 1 h at RT followed by further washes with TBST as described earlier ...
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated for 1 h in PBS with secondary antibodies: Alexa Fluor 488 donkey anti-chicken or Alexa Fluor 594 donkey anti-rabbit (1:500, Jackson ImmunoResearch). Tissue was washed 3 times in PBS ...
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bioRxiv - Neuroscience 2023Quote: ... The membranes were then incubated with a species-specific secondary antibody: anti-rabbit (Jackson Immunoresearch, 111-035-144; 1:10 000) and anti-chicken (Jackson Immunoresearch ...
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bioRxiv - Neuroscience 2023Quote: ... The sections were then rinsed in PBS and incubated for 2 hours in Alexa Fluor 488-conjugated donkey anti-guinea pig antibody (1/100, Jackson ImmunoResearch) diluted in blocking solution and washed in PBS ...
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bioRxiv - Neuroscience 2023Quote: ... followed by incubation with the primary antibodies overnight at 4°C and then the proper secondary antibodies (1:500, Alexa Fluor 488, 594, 647 conjugated; Jackson ImmunoResearch) for 2 h at room temperature in the same blocking solution ...
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bioRxiv - Neuroscience 2023Quote: ... All sections were rinsed three times for 15 min in PBS-T and incubated for 4 hours at ∼20 °C in secondary antibodies in the blocking solution (Cy3 donkey anti-guinea pig, 1:400, Jackson ImmunoResearch, catalog no ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were washed three times in PBST and incubated with secondary antibodies—Alexa Fluor 594 goat anti-rabbit IgG H&L (1:250; Jackson ImmunoResearch, West Grove ...
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bioRxiv - Cancer Biology 2023Quote: ... at a 1:25 dilu,on followed by bio,nylated goat an,-rat an,body secondary (Jackson ImmunoResearch 112-065-167) and streptavidin-HRP (Jackson ImmunoResearch 016-030-084) ...
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bioRxiv - Neuroscience 2023Quote: ... Sections used for electrophysiology (see below) were fixed overnight in AntigenFix before biocytin was revealed with Alexa647-conjugated-streptavidin (1/200, Jackson ImmunoResearch). Images of biocytin-filled neurons were acquired as 3D stacks on a confocal microscope (Leica ...
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bioRxiv - Neuroscience 2023Quote: ... Fluorescence was visualized using 30-min secondary incubation in either Alexa Fluor 488-conjugated donkey anti-rabbit or Cy3-conjugated donkey anti-mouse secondary (1:1000, Jackson Immunoresearch). Following each incubation step ...
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bioRxiv - Neuroscience 2023Quote: ... and secondary Biotin-SP-conjugated AffiniPure Donkey Anti-Rabbit IgG (H+L) at 1:250 (Jackson ImmunoResearch cat: 711-065-152).
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bioRxiv - Developmental Biology 2024Quote: ... the membranes were incubated with a peroxidase-AffiniPure Donkey α-Rabbit IgG (H+L) (Jackson ImmunoResearch, 711-035-152, 1:5,000) or Peroxidase AffiniPure Goat Anti-Rat IgG (H+L ...
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bioRxiv - Microbiology 2023Quote: ... The secondary antibodies used were 488-conjugated Affinipure Goat Anti-Rabbit IgG(H+L) (Jackson ImmunoResearch, 111-545-003, 1:1000) and 594-conjugated Goat Anti-Mouse IgG (H+L ...
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bioRxiv - Evolutionary Biology 2024Quote: ... peroxide blocked for 30m (RT) then incubated with biotinylated goat anti-rabbit secondary antibody (Jackson ImmunoResearch 111-065-144, 1:5000) for 1h (RT) ...