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Citations for Jackson ImmunoResearch :
3801 - 3850 of 3977 citations for GPN loop GTPase 3 GPN3 Antibody Biotin since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... 1:5,000 dilution in PBST) and goat anti-rabbit secondary antibody peroxidase conjugate (1:20,000 dilution in PBST; Jackson ImmunoResearch Laboratories Inc., 111-035-003).
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bioRxiv - Neuroscience 2023Quote: ... Immunostaining was performed by incubating sections in blocking solution (PBS, 10% Donkey Serum, 1% Triton-100, 1h) followed by primary (overnight, 4°C) and secondary antibodies (1-2h, Jackson ImmunoResearch or ThermoFisher Scientific) incubation ...
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bioRxiv - Cell Biology 2022Quote: ... Membranes were rinsed 3 × 5 min with TBS-T and incubated with IRDye goat-anti-mouse or goat-anti-rabbit secondary antibodies (1:10,000 Jackson ImmunoResearch Laboratories, West Grove, PA) diluted in 5% (w/v ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were rinsed with PBS and non-specific antibody binding sites were blocked with 10% normal goat serum (Jackson ImmunoResearch Laboratories, West Grove, PA) diluted in PBS (blocking buffer ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were incubated with the following secondary antibodies at the indicated dilutions: Cy3-conjugated goat anti-mouse (Jackson ImmunoResearch 115-165-003, 1:1000), Cy3-conjugated goat anti-rat (Jackson ImmunoResearch 112-165-003 ...
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bioRxiv - Cell Biology 2023Quote: ... Tissue sections were thoroughly washed and incubated with Alexa Fluor®594 (Ex: 591 nm, Em: 614 nm) conjugated secondary antibody (Jackson ImmunoResearch Lab, Inc.), at a dilution of 1:700 for 1 h at room temperature in dark ...
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bioRxiv - Cell Biology 2023Quote: ... The cells were incubated with the secondary antibody (goat-anti-mouse, FITC conjugated, 1:200 in PBSA, Jackson ImmunoResearch Europe Ltd, #115-095-146) for 1 hour at room temperature in the dark ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated for 30 min in a Horse Radish Peroxidase conjugated goat anti-guinea pig secondary antibody (1:100; Cat No. 106-035-003, Jackson ImmunoResearch; West Grove, Pennsylvania) diluted in TNB buffer ...
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bioRxiv - Immunology 2023Quote: ... HRP-conjugated anti-mouse IgG (Cat #115-035-146) and anti-mouse IgM (Cat #115-035-075) antibodies were obtained from Jackson Immunoresearch (West Grove, Pennsylvania). Anti-mouse IgM conjugated to Cy3 was from Jackson Immunoresearch (West Grove ...
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bioRxiv - Bioengineering 2023Quote: ... the beads were washed once with ice cold PBSB and incubated with a 1:300 dilution of goat anti-alpaca IgG H+L (also reactive for llama antibodies) Alexa Fluor 647 (Jackson ImmunoResearch, 128-605-160) on ice for 4 min ...
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bioRxiv - Systems Biology 2023Quote: ... Cells were then washed and stained for 1 hour at room temperature with the following secondary antibodies: anti-hamster Cy3 (Jackson ImmunoResearch 127165160, 1:500), anti-rabbit AlexFluor 488 (Invitrogen A11008 ...
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bioRxiv - Developmental Biology 2023Quote: ... The cells were then washed with PBS three times (5 minutes each) and incubated with Alexa Fluor 488 anti-mouse secondary antibody (1:1000, Jackson Immunoresearch #115-545-146) at 4°C overnight ...
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bioRxiv - Bioengineering 2024Quote: ... Next day, the cells on the slides were incubated with secondary antibodies (donkey anti-rabbit, 1:500) (Jackson ImmunoResearch, Cat. No. 711-605-152) conjugated with Cy5 for 2 h at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... washed 3 times in PB 0.1 M, a secondary antibody solution (PB 0.1M, Triton 0.3%, NGS 2%, goat anti-rabbit Cyanine Cy3TM (Jackson Immunoresearch, 111-165-003, RRID: AB_2338000), goat anti-mouse Cy5TM (Jackson Immunoresearch ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were then extensively washed with PBS and incubated for 45 minutes at 37°C in a humidified chamber with the secondary anti-rat IgG Alexa 594 antibody (1:500; Jackson ImmunoResearch, #112-585-167) diluted in 3% BSA in PBS ...
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bioRxiv - Neuroscience 2024Quote: ... The sections were then incubated in the same fresh medium containing biotinylated goat anti-rabbit IgG antibody (1:1,000; Jackson ImmunoResearch, West Grove, PA, USA) for 2 h at room temperature ...
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bioRxiv - Immunology 2024Quote: ... slides/wells were washed in 0.01-M PBS and incubated in the same blocking buffer but with appropriate secondary antibodies (647 donkey anti-guinea pig, and 594 donkey anti-rabbit, Jackson Immunoresearch, West Grove, PA). The gelatin-embedded DRG sections in the wells were then placed carefully on gelatin-coated slides ...
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bioRxiv - Immunology 2024Quote: ... goat anti-mouse secondary detection antibody conjugated to HRP was diluted 1:10,000 for IgG (peroxidase conjugated goat anti-mouse IgG H&L, Jackson ImmunoResearch, cat. 115-035-003) and 1:5,000 for IgA (peroxidase conjugated goat anti-mouse IgA ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated overnight at 4°C in primary antibody solution containing 2% Normal Donkey Serum (NDS; Jackson Immunoresearch Laboratories Inc. 017-000-121) + 1xPBS + 0.03% Triton + primary antibody (rabbit anti-P2RY12 ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were then rinsed with PBS and incubated with Cy3-conjugated goat anti-rabbit secondary antibody (Millipore Sigma, Billerica, MA; Jackson Immunoresearch, Philadelphia, PA) diluted 1:800 in BSA-PBS (1%) ...
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bioRxiv - Neuroscience 2024Quote: ... Slides were then placed in secondary antibodies, Alpha-Bungarotoxin AF555 1:500 (ThermoFischer, #B35451) and Donkey anti-Rabbit AF488 1:450 (Jackson ImmunoResearch, #711-545-152), diluted in PBS1X for 2.5 hours at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were incubated in secondary antibody solution (0.1% Triton 100X in PBS) for 2 hours at room temperature with the following secondary antibodies: Cy3 donkey anti-rabbit IgG (1:1000, Jackson ImmunoResearch, #711-165-152) and Cy5 donkey anti-guinea pig IgG (1:1000 ...
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Anatomical characterisation of somatostatin-expressing neurons belonging to the anterolateral systembioRxiv - Neuroscience 2024Quote: ... and then for 2-18 hours in appropriate species-specific secondary antibodies that were raised in donkey and conjugated to Alexa 488 or Rhodamine Red (from Jackson Immunoresearch, West Grove, PA) or to Alexa 555-plus (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2024Quote: ... sections were washed 4x in PBT and incubated for 90 min in secondary antibody (Alexafluor 488 AffiniPure Donkey Anti-Rabbit, 1:1000, Jackson Immunoresearch # 711-545-152). The secondary antibody was then washed off with PBT in 3X10 min washes ...
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bioRxiv - Neuroscience 2024Quote: ... The brain sections were rinsed for three times with PBS and incubated with the secondary antibody AlexaFluor 488 donkey anti-rabbit IgG (1:500, cat#: 711-545-152, Jackson ImmunoResearch Labs, RRID: AB_2313584) and Cy5-conjugated streptavidin (1:1000 ...
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bioRxiv - Cell Biology 2024Quote: ... Tissue sections were washed with PBS-T and incubated in the dark with the appropriate Alexa Fluor® -594 or -647 conjugated secondary antibody (1:700; Jackson ImmunoResearch Laboratories, Inc.) for one hour at room temperature ...
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bioRxiv - Biophysics 2021Quote: ... Secondary antibody: Alexa Fluor® 488 AffiniPure F(ab’)2 Fragment Donkey Anti-Mouse IgG (H+L) (Jackson ImmunoResearch Laboratories Inc., code. 715-546-151).
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bioRxiv - Cell Biology 2020Quote: ... cells immunostained for E-cadherin with Alexa Fluor 488 secondary antibody were blocked with 26 µg/ml of AffiniPure Fab Fragment Goat Anti-Mouse IgG (H+L) (Jackson ImmunoResearch, Inc, West Grove, PA) in 5% NGS in PBS overnight before being stained for LAMP1 with Alexa Fluor 594-conjugated goat anti-mouse secondary antibody (Invitrogen).
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bioRxiv - Cell Biology 2020Quote: ... Coverslips were washed with PBS and immunostained in PBG with secondary antibodies conjugated to fluorescein isothiocyanate (FITC) (donkey anti-mouse IgG; Jackson ImmunoResearch Lab, Inc., 1:125) or Cy3 (donkey anti-rabbit ...
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bioRxiv - Bioengineering 2022Quote: ... The cells were then washed and incubated at 37°C for 60 min with the appropriate secondary antibody (Alexa Fluor 488 donkey anti-rabbit IgG and Alexa Fluor 594 donkey anti-mouse IgG 1:1000; Jackson ImmunoResearch, West Grove, PA, USA) in blocking buffer prior to a final incubation with 4,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Immunology 2021Quote: ... slides were washed and incubated for 1 h at room temperature with anti-rabbit horseradish peroxidase-conjugated antibodies (Jackson ImmunoResearch #111-035-003, 1:500). Peroxidase activity was detected using 3,3’ Diaminobenzidine substrate (BD Pharmingen #550880) ...
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bioRxiv - Physiology 2020Quote: ... The secondary antibodies were peroxidase-conjugated AnffiniPure Goat Anti-Rabbit or mouse IgG H&L (Alexa Fluor® 488; Jackson ImmunoResearch Laboratories, West Grove, PA) at 1:500 dilution ...
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bioRxiv - Cancer Biology 2020Quote: ... and secondary antibodies diluted in blocking buffer were applied for 1 hour at RT (TRITC for α-SMA; Cy5 for fibronectin, Jackson ImmunoResearch, West Grove, PA). Next ...
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bioRxiv - Biochemistry 2021Quote: ... blots were incubated 1 h at room temperature with goat anti-rabbit (1:10000) or anti-mouse (1:5000) HRP-conjugated secondary antibodies (Jackson ImmunoResearch Europe Ltd, Newmarket, UK) and signal was detected with WesternBright ECL Spray (Advansta ...
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bioRxiv - Bioengineering 2021Quote: ... Cells were then incubated at 37°C for 60 min with a secondary antibody (Alexa Fluor 594 donkey anti-rabbit IgG and Alexa Fluor 594 donkey anti-mouse IgG, 1:1000; Jackson ImmunoResearch, West Grove, PA, USA) in blocking buffer before final incubation with 4,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Cell Biology 2020Quote: ... Immunoreactivity was detected using the following secondary peroxidase conjugated antibodies: goat anti-rabbit and anti-mouse IgG-HRP (111-035-003, 115-035-003; Jackson ImmunoResearch Laboratories, Inc. Cambridge, UK) and enhanced chemiluminescent (ECL ...
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bioRxiv - Molecular Biology 2022Quote: ... Primary antibody was washed in PBS with 0.1% Tween 20 (PBT) before applying a horseradish peroxidase (HRP) conjugated anti-guinea pig antibody (Jackson ImmunoResearch Laboratories, INC., West Grove, PA) at a final concentration of 1:250 for 2 hrs at RT or ON at at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were washed three times with diluted blocking buffer (15 min each) before incubation with secondary antibodies (Ch-647: Jackson Immunoresearch, Cat# 703-605-155) for 1 hr ...
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bioRxiv - Neuroscience 2021Quote: ... Primary antibody incubation was followed by washes and incubation with secondary antibody for 1h (HRP-conjugated goat anti-rabbit IgG, and HRP-conjugated goat anti-mouse; Jackson ImmunoResearch Laboratories, West Grove, PA). Protein bands were visualized using the ECL detection system (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... and subsequently with the fluorophore-conjugated secondary antibodies (Figure 1B5): Alexa Fluor® 488 AffiniPure Donkey Anti-Chicken 1:000 (Jackson ImmunoResearch, 703-545-155), DyLight™ 405 AffiniPure Donkey Anti-Guinea pig IgG (H+L ...
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bioRxiv - Neuroscience 2020Quote: ... followed by 2h incubation in species-specific secondary antibody directly conjugated to fluorophores (Cy3 or Cy5 from Jackson ImmunoResearch or Alexa Fluor 488 from Invitrogen). Sections were mounted in Mowiol (Calbiochem ...
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bioRxiv - Neuroscience 2021Quote: ... above tissue sections under ISH were incubated with chicken anti-NeuN antibody (1:200; Aves, Davis, CA) overnight at 4°C followed by donkey anti-chicken IgG Alexa 488 (Jackson ImmunoResearch labs, West Grove, PA) for 1 hr at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... Brains were stained with Th antibody (Santa Cruz Biotechnology, #sc-25269; 1/20 dilution; anti-mouse IgG2a secondary Fab fragment conjugated with Alexa 594 (Jackson ImmunoResearch, #115-587-186)) and nuclear staining (SYTOX-G) ...
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bioRxiv - Neuroscience 2020Quote: ... Brains were stained with β-Amyloid (6E10) antibody (Biolegend, #93049; 1/100 dilution; anti-mouse IgG1 secondary Fab fragment conjugated with Alexa 594 (Jackson ImmunoResearch, #115-587-185)) and nuclear staining (SYTOX-G).
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bioRxiv - Neuroscience 2020Quote: ... sections were rinsed in PBS and then incubated for 1 hr in the appropriate biotinylated secondary antibody (anti-mouse or anti rabbit, 1:600 Jackson ImmunoResearch, West Grove, PA, USA) in PBS with 0.4% Triton-X 100 ...
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bioRxiv - Bioengineering 2021Quote: ... strips were washed 10 times with PBST and the binding of VHH-Fcs to denatured S was probed by incubating strips with 1 mL of 100 ng/mL anti-human Ig Fc antibody-peroxidase conjugate (Jackson ImmunoResearch, Cat#016-030-084) at room temperature for 1 h ...
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bioRxiv - Neuroscience 2021Quote: ... sections were incubated for 2 h at room temperature with a secondary antibody conjugated to 6-nm gold particles (Jackson ImmunoResearch, cat # 115-195-146) diluted to 1:40 in blocking buffer ...
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bioRxiv - Neuroscience 2021Quote: ... the sections were incubated for 1 h at 35°C with a secondary antibody conjugated to 6-nm gold particles (1:40 dilution, Jackson ImmunoResearch, cat # 111-195-144) and a secondary antibody conjugated to 12-nm gold particles (1:20 dilution ...
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bioRxiv - Bioengineering 2021Quote: ... The channels were incubated at 25 °C for 60 min with the corresponding secondary antibodies AlexaFluor 594 donkey anti-rabbit IgG (1:1000; Jackson ImmunoResearch, West Grove, PA, USA) and Alexa Fluor 648 donkey anti-rat IgG (1:1000 ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were rinsed in PBS and incubated at 4°C in PBS containing 2% donkey serum and secondary antibodies conjugated to anti-rabbit Cy3 (Jackson ImmunoResearch Inc., West Grove, PA) and anti-guinea pig CF405M (Biotium ...