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Citations for Jackson ImmunoResearch :
3701 - 3750 of 3977 citations for GPN loop GTPase 3 GPN3 Antibody Biotin since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... the membranes were incubated with horseradish peroxidase (HRP)-conjugated secondary antibodies: donkey anti-rabbit IgG-HRP (Jackson ImmunoResearch, 711-035-152; 1:5,000); donkey anti-mouse IgG-HRP (Jackson ImmunoResearch ...
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bioRxiv - Neuroscience 2024Quote: ... the tissue was washed 3 times for 5 min each with 1X PBS + 0.2% Triton-X and then incubated overnight in blocking buffer containing Alexa-Fluor-conjugated secondary antibodies (Jackson ImmunoResearch Laboratories, 1:500). Upon completion of incubation with the secondary antibodies ...
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bioRxiv - Neuroscience 2024Quote: ... sections were rinsed and incubated for 2h at RT with donkey anti-mouse Cy3 or donkey anti-rabbit Alexa 488 secondary antibodies (Jackson Immunoresearch, Suffolk, UK). Finally ...
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bioRxiv - Immunology 2024Quote: ... Then sections were washed and incubated with 7.5 μg/ml secondary AlexaFluor 488 conjugated polyclonal goat anti-rabbit IgG antibodies (Jackson ImmunoResearch, 111-545-003) in 10% NGS-TBS-1% BSA ...
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bioRxiv - Neuroscience 2024Quote: Identification of different cells and structures in spinal cord and brain was performed using primary antibodies diluted in PBS blocking solution containing 10% normal donkey serum (Jackson ImmunoResearch, Cambridge, UK) and 0.3% Triton-X100 (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2024Quote: ... the brain sections were then incubated with a secondary antibody (Rhodamine-conjugated donkey-anti-goat, # 705-025-147, 1:200, Jackson ImmunoResearch, RRID: AB_2340389) for 2 hours at RT ...
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bioRxiv - Neuroscience 2024Quote: Identification of different cells and structures in the brain was performed using primary antibodies diluted in a PBS-blocking solution containing 10% normal donkey serum (Jackson ImmunoResearch, Cambridge, UK) and 0.3% Triton-X100 (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2024Quote: ... Tissue sections were then incubated for 2 hours in the secondary antibody solution consisting of Alexa Fluor® 488 donkey anti-chicken (1:250; Jackson ImmunoResearch, AB_2340375), diluted in TBS containing 5% donkey serum ...
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bioRxiv - Neuroscience 2024Quote: ... the slices were incubated at RT for 1 h with a biotinylated goat anti-rabbit antibody (1:500; 111-065-144, Jackson ImmunoResearch Laboratories; RRID:AB_2337965) and then washed again three times in PBS for 10 min each ...
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bioRxiv - Biochemistry 2024Quote: ... the membrane was washed three times with TBS-T and an Alexa Fluor 680-conjugated goat anti-human secondary antibody (1:50,000 dilution, Jackson ImmunoResearch, 109-625-098) was added and incubated during 1 h at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... RRID: AB_562180)] and secondary antibodies [Alexa Fluor 488 donkey anti-mouse IgG (1:500, cat#715-545-150; Jackson ImmunoResearch Labs, RRID: AB_2340846) and Alexa Fluor 647 donkey anti-rabbit IgG (1:500 ...
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bioRxiv - Biochemistry 2024Quote: ... Membranes were washed three times with TBS-T and an Alexa Fluor 680-conjugated goat anti-human secondary antibody (1:50,000 dilution, Jackson ImmunoResearch, 109-625-098) was added and incubated during 1 h at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... the slides/dishes were treated with the corresponding secondary antibody: either Dye light 488-labeled anti-rabbit IgG (Jackson ImmunoResearch; dilution, 1/2000) or Alexa fluor 546-labeled anti-mouse IgG (Molecular Probes ...
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bioRxiv - Cell Biology 2024Quote: ... Coverslips were washed 3 × 5 min with PBS and incubated for 1 hour at room temperature in blocking solution containing the following donkey polyclonal secondary antibodies conjugated to Alexa dyes (Jackson ImmunoResearch; 1:300): anti-mouse IgG Alexa 488 (715-545-150) ...
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bioRxiv - Cell Biology 2024Quote: ... 0.05% Tween 20 and 0.05% sodium azide in 20 mM Tris-HCl (pH 8.0) and incubated with secondary antibodies conjugated with 18-nm colloidal gold (Jackson ImmunoResearch; 111-215-144) diluted (1:10 ...
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bioRxiv - Developmental Biology 2021Quote: ... The secondary antibodies were Alexa Fluor® 488 conjugated anti-rabbit and Alexa Fluor® 488 conjugated anti-mouse (Jackson ImmunoResearch, 1:200 diluted). After staining ...
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bioRxiv - Developmental Biology 2021Quote: ... Blots were incubated in 1:500 mouse anti-Myc (CST #2276) and 1:3000 goat anti-mouse HRP secondary antibody (Jackson ImmunoResearch #115-035-003). For the loading control ...
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bioRxiv - Developmental Biology 2020Quote: ... the embryos were incubated overnight at 4 °C in Cy3-conjugated goat anti-rabbit antibody (1:500 in BB; Jackson Immunoresearch, 111-165-144), Alexa488-conjugated goat anti-mouse (1:500 in BB ...
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bioRxiv - Physiology 2022Quote: ... followed by incubation with secondary Alexa-488-conjugated donkey anti-rabbit or Cy3-conjugated goat anti-mouse antibody (Jackson ImmunoResearch Laboratories, West Grove, PA) for 30 min ...
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bioRxiv - Neuroscience 2021Quote: ... Secondary antibodies (CY5 Donkey Anti-Mouse; Jackson ImmunoResearch Labs; Cat# 715-175-151. CY3 Donkey Anti-Chicken; Jackson ImmunoResearch Labs; Cat#703-165-155) were spun in a centrifuge for 10 minutes at 13000 rpm ...
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bioRxiv - Molecular Biology 2020Quote: ... the coverslips were incubated with Fab fragment of goat anti-mouse IgG (H+L) secondary antibody conjugated to Alexa Fluor-594 (Jackson Immunoresearch, West Grove, PA) for 1 h at RT ...
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bioRxiv - Cell Biology 2022Quote: ... Alexa Fluor 488-conjugated goat-anti-rabbit (1:500) and Alexa Fluor 647-conjuated goat-anti-mouse (1:800) secondary antibodies (Jackson Immunoresearch; West Grove, PA) were diluted in a solution consisting of 2% goat serum ...
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bioRxiv - Cell Biology 2022Quote: ... Mouse tissue sections incubated with mouse monoclonal antibodies were first incubated with a solution containing donkey anti-mouse IgG AffiniPure Fab fragments (1:25 in PBS; Jackson ImmunoResearch #715-007-003) for one hour prior to blocking ...
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bioRxiv - Systems Biology 2020Quote: ... The following secondary antibodies were used: goat anti-Rabbit IgG-Alexa 594 or Alexa 647 (Jackson ImmunoResearch, cat 111-586-003, 111-606-003), goat anti-Rat IgG-Alexa 488 (Jackson ImmunoResearch ...
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bioRxiv - Immunology 2021Quote: ... Plates were developed using 15μl/well of combination of horseradish peroxidase–conjugated antibodies in assay diluent comprising) goat anti-human IgG (Jackson ImmunoResearch Laboratories,109-035-098) at 1:10,000 dilution ...
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bioRxiv - Cell Biology 2021Quote: ... Secondary antibodies used included peroxidase-conjugated donkey anti-mouse IgG and peroxidase-conjugated goat anti-mouse IgM (all from Jackson ImmunoResearch; diluted 1:10,000). Enhanced chemiluminescence was detected using the Western Blotting Luminol Reagent (Santa Cruz Biotechnology ...
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bioRxiv - Neuroscience 2020Quote: ... The slides were incubated for 30 minutes in a 1:200 dilution of the goat anti-rabbit secondary antibody conjugated to horse radish peroxidase (Jackson ImmunoResearch #111-036-003). The brown color was produced by reaction with 3,3’-diaminobenzidine chromogen (DAB ...
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bioRxiv - Cell Biology 2021Quote: ... Slides were washed with PBS and then stained with secondary antibodies donkey anti-rabbit-AF488 and donkey anti-mouse-AF647 (1:1000, Jackson ImmunoResearch, West Grove, Pennsylvania) for one hour at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... and the following secondary antibodies at 4°C for 24 h: Alexa Fluor 488 donkey anti-chicken (1:250, Jackson ImmunoResearch, #703-545-155) and Alexa Fluor 568 donkey anti-rabbit (1:250 ...
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bioRxiv - Neuroscience 2020Quote: ... followed by 2-h room temperature incubation in Alexa 647 donkey anti-rabbit antibody (part number 711-606-152, Jackson ImmunoResearch, West Grove, PA). Sections were then washed and processed using 4”,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Biophysics 2020Quote: ... In both cases secondary staining was done by 1 h treatment at room temperature with a mix of donkey anti-mouse DNA-conjugated secondary fragment antibody (Jackson ImmunoResearch, 715-007-003) and donkey anti-rabbit DNA-conjugated secondary fragment antibody (Jackson ImmunoResearch ...
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bioRxiv - Cell Biology 2021Quote: ... The embryos were washed twice in 4X SSC / 2% BSA / 0.05% Tween20 before and after incubation with the secondary antibody (mouse anti-sheep Ig coupled to FITC, 1/200, 713-095-147, Jackson ImmunoResearch Laboratories Inc., USA) for 1h at RT ...
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bioRxiv - Cell Biology 2020Quote: ... Blots were washed 3X with TBS containing 0.1% Tween-20 and incubated for 1 hr RT with secondary antibody conjugated to horseradish peroxidase diluted into blocking buffer (Jackson ImmunoResearch—West Grove, PA). Blots were washed a final 3X and imaged using Clarity Western ECL substrate (BioRad ...
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bioRxiv - Molecular Biology 2022Quote: ... by wet transfer and imaging was done using X-ray film (FujiFilm) to detect signal from HRP-conjugated anti-mouse secondary antibody (1:7500, Jackson ImmunoResearch, #111-035-003) incubated with ECL substrate (Thermo Scientific) ...
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bioRxiv - Cancer Biology 2022Quote: ... Coverslips were washed with PBS 1X three times for 5 min at RT and incubated with Donkey anti-mouse IgG Alexa Fluor 488 secondary antibody (1:200; Jackson ImmunoResearch, 715-095-150) and Rhodamine-conjugated Phalloidin (Sigma ...
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bioRxiv - Neuroscience 2022Quote: ... The following day the sections were rinsed with 0.1M PBS and incubated with the appropriate fluorescent secondary antibodies (anti-mouse or anti-rabbit 1:1000; Jackson ImmunoResearch, West Grove, PA, USA) for 1h at RT ...
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bioRxiv - Microbiology 2022Quote: ... followed by the addition of HRP-conjugated goat anti-Syrian hamster IgG secondary antibody (Jackson Immunoresearch, Cat# 107-035-142, 1:8000 in PBST) or mouse anti-hamster-IgG2/3-HRP (Southern Biotech ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and 488/500-550 nm (green) for GFP or the donkey anti-sheep antibody conjugated to Alexa Fluor 488 (Jackson ImmunoResearch #713-545-147). Laser intensities ...
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bioRxiv - Neuroscience 2020Quote: ... Brains were stained by c-Fos antibody (CST, #2250S; 1/75 dilution; anti-rabbit IgG secondary Fab fragment conjugated with Alexa 594 (Jackson ImmunoResearch, #111-587-008)) and nuclear staining dye (SYTOX-G).
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bioRxiv - Neuroscience 2020Quote: ... Brains were stained with PV antibody (Swant, #PV235; 1/50 dilution; anti-mouse IgG1 secondary Fab fragment conjugated with Cy3 (Jackson ImmunoResearch, #115-167-185)) ...
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bioRxiv - Neuroscience 2020Quote: ... Brains were stained with ChAT antibody (abcam, #ab178850; 1/200 dilution; anti-rabbit IgG secondary Fab fragment conjugated with Alexa 594 (Jackson ImmunoResearch, #111-587-008)) and nuclear staining (SYTOX-G ...
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bioRxiv - Neuroscience 2020Quote: ... the sections were incubated for 4-6 h in the following secondary antibodies: donkey anti-rat 488 (1: 500; Jackson Immunoresearch, 712-546-153), donkey anti-rabbit-Cy3 (1:500 ...
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bioRxiv - Microbiology 2021Quote: ... followed by the addition of HRP-conjugated goat anti-Syrian hamster IgG secondary antibody (Jackson immunoresearch, Cat# 107-035-142, 1:8000 in PBST) or mouse anti-hamster-IgG2/3-HRP (Southern Biotech ...
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Serotonin Modulates an Inhibitory Input to the Central Amygdala from the Ventral Periaqueductal GraybioRxiv - Neuroscience 2022Quote: ... and then incubated in a secondary antibody solution made with donkey anti-chicken IgY conjugated to Alexa-Fluor 488 (Jackson Immunoresearch, West Grove, PA) diluted 1:200 in PBS for 2 hours ...
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bioRxiv - Cell Biology 2022Quote: ... specimens were washed three times for 90 min in PBS-T and incubated overnight with anti-rabbit fluorescein isothiocyanate-conjugated (FITC) secondary antibody (Jackson ImmunoResearch, 711-095-152) diluted 1:200-250 ...
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bioRxiv - Cell Biology 2022Quote: ... The primary antibody was rabbit monoclonal anti-mouse caveolin-1 (1:100) and Alexa Fluor® 647 Fab1 fragment goat anti-rabbit (Jackson Immunoresearch; 1:100) was used as the secondary to further improve spatial resolution.
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bioRxiv - Cancer Biology 2020Quote: ... Detection was done with either HRP-conjugated goat anti-mouse or anti-rabbit secondary antibodies (Jackson ImmunoResearch cat#211-032-171, 111-035-144), followed by enhanced chemiluminescence (EZ-ECL ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The membrane was washed three times with TBST for 10 min and incubated for 1 hour with 1:10,000 diluted peroxidase-conjugated anti-mouse or anti-rabbit antibody (Jackson ImmunoResearch, West Grove, PA, USA) in 5% skim milk in TBST ...
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bioRxiv - Genetics 2020Quote: ... membranes were incubated for 1 hr at room temperature with species-specific horseradish peroxidase conjugated secondary antibodies (1:10000, Jackson ImmunoResearch, West Grove, PA) diluted in 2% non-fat milk in TBST ...
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bioRxiv - Cell Biology 2021Quote: ... derivatized as described previously,52 with anti-hu Fc mixed with equimolar anti-mu Fc antibodies (cat # 109-005-008 and 115-005-008, respectively; Jackson ImmunoResearch, West Grove, PA) at pH 5.0 ...