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Citations for Jackson ImmunoResearch :
3751 - 3800 of 3903 citations for Ubiquitin Conjugating Enzyme E2 K UBE2K Antibody FITC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... slides were washed and incubated for 1 h at room temperature with anti-rabbit horseradish peroxidase-conjugated antibodies (Jackson ImmunoResearch #111-035-003, 1:500). Peroxidase activity was detected using 3,3’ Diaminobenzidine substrate (BD Pharmingen #550880) ...
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bioRxiv - Physiology 2020Quote: ... The secondary antibodies were peroxidase-conjugated AnffiniPure Goat Anti-Rabbit or mouse IgG H&L (Alexa Fluor® 488; Jackson ImmunoResearch Laboratories, West Grove, PA) at 1:500 dilution ...
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bioRxiv - Cancer Biology 2020Quote: ... and secondary antibodies diluted in blocking buffer were applied for 1 hour at RT (TRITC for α-SMA; Cy5 for fibronectin, Jackson ImmunoResearch, West Grove, PA). Next ...
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bioRxiv - Biochemistry 2021Quote: ... blots were incubated 1 h at room temperature with goat anti-rabbit (1:10000) or anti-mouse (1:5000) HRP-conjugated secondary antibodies (Jackson ImmunoResearch Europe Ltd, Newmarket, UK) and signal was detected with WesternBright ECL Spray (Advansta ...
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bioRxiv - Bioengineering 2021Quote: ... Cells were then incubated at 37°C for 60 min with a secondary antibody (Alexa Fluor 594 donkey anti-rabbit IgG and Alexa Fluor 594 donkey anti-mouse IgG, 1:1000; Jackson ImmunoResearch, West Grove, PA, USA) in blocking buffer before final incubation with 4,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Cell Biology 2020Quote: ... Immunoreactivity was detected using the following secondary peroxidase conjugated antibodies: goat anti-rabbit and anti-mouse IgG-HRP (111-035-003, 115-035-003; Jackson ImmunoResearch Laboratories, Inc. Cambridge, UK) and enhanced chemiluminescent (ECL ...
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bioRxiv - Molecular Biology 2022Quote: ... Primary antibody was washed in PBS with 0.1% Tween 20 (PBT) before applying a horseradish peroxidase (HRP) conjugated anti-guinea pig antibody (Jackson ImmunoResearch Laboratories, INC., West Grove, PA) at a final concentration of 1:250 for 2 hrs at RT or ON at at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... After three washes with PBS the sample was incubated for 3-4h with a secondary donkey anti-rabbit antibody (711-005-152; Jackson ImmunoResearch, West Grove, PA, USA) that was conjugated to Alexa Fluor 647-NHS at an average degree of labeling of 1.5 ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were washed three times with diluted blocking buffer (15 min each) before incubation with secondary antibodies (Ch-647: Jackson Immunoresearch, Cat# 703-605-155) for 1 hr ...
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bioRxiv - Neuroscience 2021Quote: ... Primary antibody incubation was followed by washes and incubation with secondary antibody for 1h (HRP-conjugated goat anti-rabbit IgG, and HRP-conjugated goat anti-mouse; Jackson ImmunoResearch Laboratories, West Grove, PA). Protein bands were visualized using the ECL detection system (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... and subsequently with the fluorophore-conjugated secondary antibodies (Figure 1B5): Alexa Fluor® 488 AffiniPure Donkey Anti-Chicken 1:000 (Jackson ImmunoResearch, 703-545-155), DyLight™ 405 AffiniPure Donkey Anti-Guinea pig IgG (H+L ...
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bioRxiv - Neuroscience 2020Quote: ... followed by 2h incubation in species-specific secondary antibody directly conjugated to fluorophores (Cy3 or Cy5 from Jackson ImmunoResearch or Alexa Fluor 488 from Invitrogen). Sections were mounted in Mowiol (Calbiochem ...
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bioRxiv - Neuroscience 2021Quote: ... above tissue sections under ISH were incubated with chicken anti-NeuN antibody (1:200; Aves, Davis, CA) overnight at 4°C followed by donkey anti-chicken IgG Alexa 488 (Jackson ImmunoResearch labs, West Grove, PA) for 1 hr at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... Brains were stained with Th antibody (Santa Cruz Biotechnology, #sc-25269; 1/20 dilution; anti-mouse IgG2a secondary Fab fragment conjugated with Alexa 594 (Jackson ImmunoResearch, #115-587-186)) and nuclear staining (SYTOX-G) ...
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bioRxiv - Neuroscience 2020Quote: ... Brains were stained with β-Amyloid (6E10) antibody (Biolegend, #93049; 1/100 dilution; anti-mouse IgG1 secondary Fab fragment conjugated with Alexa 594 (Jackson ImmunoResearch, #115-587-185)) and nuclear staining (SYTOX-G).
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bioRxiv - Neuroscience 2020Quote: ... sections were rinsed in PBS and then incubated for 1 hr in the appropriate biotinylated secondary antibody (anti-mouse or anti rabbit, 1:600 Jackson ImmunoResearch, West Grove, PA, USA) in PBS with 0.4% Triton-X 100 ...
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bioRxiv - Bioengineering 2021Quote: ... strips were washed 10 times with PBST and the binding of VHH-Fcs to denatured S was probed by incubating strips with 1 mL of 100 ng/mL anti-human Ig Fc antibody-peroxidase conjugate (Jackson ImmunoResearch, Cat#016-030-084) at room temperature for 1 h ...
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bioRxiv - Neuroscience 2021Quote: ... sections were incubated for 2 h at room temperature with a secondary antibody conjugated to 6-nm gold particles (Jackson ImmunoResearch, cat # 115-195-146) diluted to 1:40 in blocking buffer ...
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bioRxiv - Neuroscience 2021Quote: ... the sections were incubated for 1 h at 35°C with a secondary antibody conjugated to 6-nm gold particles (1:40 dilution, Jackson ImmunoResearch, cat # 111-195-144) and a secondary antibody conjugated to 12-nm gold particles (1:20 dilution ...
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bioRxiv - Bioengineering 2021Quote: ... The channels were incubated at 25 °C for 60 min with the corresponding secondary antibodies AlexaFluor 594 donkey anti-rabbit IgG (1:1000; Jackson ImmunoResearch, West Grove, PA, USA) and Alexa Fluor 648 donkey anti-rat IgG (1:1000 ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were rinsed in PBS and incubated at 4°C in PBS containing 2% donkey serum and secondary antibodies conjugated to anti-rabbit Cy3 (Jackson ImmunoResearch Inc., West Grove, PA) and anti-guinea pig CF405M (Biotium ...
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bioRxiv - Neuroscience 2021Quote: ... AffiniPure minimally cross-reactive secondary antibodies used for immunofluorescent imaging were conjugated to Alexa Fluor (594, 488, 350) or AMCA (Jackson ImmunoResearch Laboratories, West Grove, PA). Secondary antibodies used for western immunoblotting were conjugated to horseradish peroxidase (Jackson ImmunoResearch Laboratories) ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells were then washed 3x with PBS and incubated with the following secondary antibodies in PBS: anti-goat Alexa Fluor® 488 (1:500, Jackson ImmunoResearch, #705-546-147), anti-rabbit Alexa Fluor® 555 (1:500 ...
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bioRxiv - Neuroscience 2021Quote: ... sections were rinsed in fresh PBS and kept in the secondary antibodies solution (donkey anti-goat Alexa Fluor 467 and donkey anti-mouse Cy3, 1:300; Jackson ImmunoResearch, West Grove, PA, USA) overnight at 4°C ...
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bioRxiv - Immunology 2021Quote: ... live cells expressing S delta 19aa were suspended in QSol Buffer (IntelliCyte Corporation) to which a cocktail of Fc-specific secondary antibodies was added: AF647 goat anti-human IgG (Jackson ImmunoResearch, Cat # 109-605-008), AF488 goat anti-human IgA (Jackson ImmunoResearch ...
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bioRxiv - Genetics 2020Quote: ... raised against a N-terminal peptide of the human myocilin protein (anti-TNT) [14] followed by incubation with a Cy2 goat anti-chicken IgY (1:1000) secondary antibody (703-225-155, Jackson ImmunoResearch, West Grove, PA, USA). Whole embryos were counterstained with DAPI (D8417 ...
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bioRxiv - Bioengineering 2023Quote: ... resuspended in 100 µL of FACS buffer containing secondary R-PE Goat Anti-Human IgG antibody diluted 1:100 (Jackson ImmunoResearch, cat #109-117-008), and incubated for 30 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... sections were washed and then incubated 3 h with a secondary antibodies cocktail of Cy3 donkey anti-rabbit (1:600; Jackson ImmunoResearch; #711-165-152, AB_2307443) and Cy2 donkey anti-chicken (1:400 ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated with appropriate fluorescently conjugated secondary antibodies (Jackson ImmunoResearch anti-guinea pig AlexaFluor647; 1:400 or Jackson ImmunoResearch antirabbit AlexaFluor594; 1:400). Slides were coverslipped in Fluro-Gel (Electron Microscopy Sciences ...
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bioRxiv - Neuroscience 2023Quote: ... donkey anti-goat or anti-mouse biotinylated secondary antibodies were incubated for 2 hours at room temperature (1:1000, Jackson ImmunoResearch, West Grove, PA, USA), followed by incubation with VECTASTAIN Elite ABC HRP solution (Vector ...
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bioRxiv - Neuroscience 2023Quote: ... then rinsed and incubated for 1 hour at RT in biotinylated donkey anti-rabbit antibody (1:250, 711-065-152, Jackson Immunoresearch Laboratories, West Grove, PA) in PBS ...
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bioRxiv - Neuroscience 2022Quote: ... and incubated in the following secondary antibodies for 1.5 h at room temperature: donkey anti chicken-A488 (1:2000, Jackson Immunoresearch cat. No. 703-545-155), donkey anti rabbit-A555 (1:2000 ...
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bioRxiv - Cell Biology 2022Quote: ... Embryos were then incubated in PBST-BSA with either Alexa fluor 546 or Alexa fluor 488 anti-mouse secondary antibody (Jackson ImmunoResearch, Ely, UK; 1:1000) at RT for 1-2 hours ...
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bioRxiv - Neuroscience 2022Quote: ... sections were rinsed in PBS and then incubated for 1 hr in a biotinylated secondary antibody (anti-rabbit, 1:600 Jackson ImmunoResearch, West Grove, PA, USA) in PBS with 0.4% Triton-X 100 ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were then washed three times and incubated for 2 h at room temperature with fluorescent secondary antibodies: Cy3 goat anti-rabbit (1/200) and/or AlexaFluor 488 goat anti-mouse (1/200; both from Jackson ImmunoResearch, West Grove, PA, USA). No signal was detected in control sections incubated in the absence of the primary antibody ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were then incubated with secondary antibodies Alexa Fluor 488-AffiniPure Goat Anti-Mouse IgG (H+L) (Jackson ImmunoResearch Labs Cat# 115-545-003, RRID:AB_2338840) and Alexa Fluor 647-AffiniPure Goat Anti-Rabbit IgG (H+L ...
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bioRxiv - Plant Biology 2024Quote: PIN1 localization in embryos transgenic for 35Sp:EGFP-ENP used PIN1 primary rabbit antibody incubation (1:1000; 4h, 37°C) and secondary rabbit Cy3-Antibodies (BSA/PBS for 3.5h at 37°C; Jackson ImmunoResearch/USA supplied by Dianova/Hamburg). After repeated washes with PBS and H20 the embryos were embedded in Citifluor antifadent mounting medium and covered with a coverslip ...
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bioRxiv - Neuroscience 2023Quote: ... sections were washed 4 x 5 min in 0.1 M PB then incubated with a biotinylated secondary antibody (biotinylated donkey anti-mouse, Jackson ImmunoResearch, 715-065-151, 1:500) for 1 hour at RT ...
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bioRxiv - Genetics 2023Quote: ... The secondary antibodies used are Alexa Fluor 647 anti-Rat IgG and Cy3 anti-Mouse IgG (Jackson Immunoresearch, 712-605-153 and 715-165-151). The dyes Phalloidin-Atto-488 (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were then incubated at 37°C for 60 min with a secondary antibody (Alexa Fluor 488 donkey anti-rabbit IgG and Alexa Fluor 594 donkey anti-mouse IgG 1:1000; Jackson ImmunoResearch, West Grove, PA, USA) in blocking buffer prior to a final incubation with 4′,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Neuroscience 2023Quote: ... tissue was washed 4×10 minutes in PBS before being incubated for two hours in secondary antibody diluted in PBS (at 1:200) [Donkey anti-rabbit Cy2 (Jackson Immunoresearch, Cat#711-225-152)] ...
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bioRxiv - Immunology 2023Quote: ... Grids were washed several times with PBS and incubated with 12-nm gold-labeled anti-human secondary antibody (Jackson ImmunoResearch, diluted 1:40 in PBS) for 1 h ...
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bioRxiv - Neuroscience 2023Quote: ... and incubated for one hour in a biotinylated secondary antibody (1:600, Biotin-SP AffiniPure Goat Anti-Rabbit IgG, Jackson Immunoresearch Labs; 111-065-003) in 0.4% Triton-X 100 in PBS ...
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bioRxiv - Neuroscience 2022Quote: ... All blots were processed for chemiluminescence by incubation with HRP-conjugated donkey anti-mouse secondary antibodies (Jackson ImmunoResearch cat. no. 715-035-151, 1:75,000) for 2 hours at 22°C ...
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bioRxiv - Bioengineering 2022Quote: ... After washing membranes 5x in TBS-T membranes were incubated with appropriate horseradish peroxidase (HRP)-conjugated secondary antibodies (1:5000, Jackson ImmunoResearch West Grove, PA, USA) for 1h at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were washed 3×5 min with PBS and stained with 1:1,000 donkey anti-chicken Alexa Fluor 488 secondary antibody (Jackson ImmunoResearch #703-545-155, RRID: AB_2340375), rinsed with PBS ...
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bioRxiv - Neuroscience 2022Quote: ... sections were rinsed in five times in PBS and then incubated for 1 hr in biotinylated secondary antibody (anti rabbit, 1:600 Jackson Immunoresearch, West Grove, PA, USA) in PBS with 0.4% Triton-X 100 ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were rinsed 3 × 5 min with PBS and incubated with AlexaFluor-labeled secondary goat-anti-mouse or goat-anti-rabbit antibodies (1:350, Jackson ImmunoResearch Laboratories, West Grove, PA) diluted in blocking buffer for 2 h at RT ...
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bioRxiv - Cancer Biology 2023Quote: ... and incubated with cells at 250 ng/mL for at least 48 hours in combination with an anti-mouse IgG secondary antibody from Jackson Immunoresearch (catalog #115-006-071) at 1 ug/mL ...
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Lysyl oxidase regulates epithelial differentiation and barrier integrity in eosinophilic esophagitisbioRxiv - Cell Biology 2023Quote: ... at 4°C overnight and then with Cy2 or Cy5-AffiniPure Donkey Anti-Mouse IgG (H+L) secondary antibody (1:500; 715-225-150 or 715-175-150; Jackson ImmunoResearch, West Grove, PA, USA) at room temperature for 1 h ...