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Citations for Jackson ImmunoResearch :
3701 - 3750 of 3903 citations for Ubiquitin Conjugating Enzyme E2 K UBE2K Antibody FITC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... cells were incubated with PBS three times as previously described in this section followed by incubation with P/B/T containing goat anti-rabbit antibody conjugated to Alexa Fluor 647 (Jackson ImmunoResearch, 111-605-144) for 2 hours at room temperature protected from light ...
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bioRxiv - Developmental Biology 2023Quote: ... Embryos were washed four times with PBST and incubated with donkey anti-goat Cy3 tertiary antibody (1:500, Jackson ImmunoResearch, catalog # 705-165-147) in PBST overnight at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were subsequently washed with saponin buffer or FACS buffer and incubated for 1 hr with secondary antibodies: goat anti-rabbit Alexa 488 (1:500, Jackson Immunoresearch, 111-545-144) or goat anti-mouse IgM-conjugated Alexa 488 (1:500 ...
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated overnight at 4°C in primary antibody solution containing 2% Normal Donkey Serum (NDS; Jackson Immunoresearch Laboratories Inc. 017-000-121) + 1xPBS + .03% Triton + primary antibodies (chicken anti-Iba1 ...
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bioRxiv - Molecular Biology 2023Quote: ... then for one hour with a horse-radish peroxidase-conjugated goat anti-mouse or anti-rabbit antibody (Jackson ImmunoResearch Laboratories, West Grove, PA, USA). After washing ...
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bioRxiv - Neuroscience 2023Quote: ... the tissue was washed six times in PBS (5 min each) then incubated with a biotinylated goat anti-rabbit antibody (1:500; Jackson ImmunoResearch Laboratories; RRID: AB_2337965) prepared in NDS for 1 hr (RT) ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were rinsed three times in 1x PBS and incubated in secondary antibodies of 1:500 goat anti-rabbit Alexa Fluor 647 (Jackson ImmunoResearch; 111-605-144) or 1:500 donkey anti-chicken Alexa Fluor 488 (Jackson ImmunoResearch ...
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bioRxiv - Neuroscience 2023Quote: ... Slides were then washed 4 × 30s in a tris buffer solution and the secondary biotinylated mouse adsorbed donkey anti-rabbit antibody (Jackson ImmunoResearch, 711-065-152) applied to all sections and incubated for 30 min ...
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bioRxiv - Neuroscience 2023Quote: ... They were then incubated overnight at room temperature with a secondary antibody solution consisting of 12-nm Colloidal Gold Affini Pure Goat Anti-Rabbit IgG (Jackson ImmunoResearch, 111-205-144) at a 1:30 dilution in the blocking solution ...
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bioRxiv - Neuroscience 2023Quote: ... The sections were then incubated in the same fresh medium containing biotinylated goat anti-rabbit IgG antibody (1:1,000; Jackson ImmunoResearch, West Grove, PA, USA) for 2 h at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... CAR expression post-transduction was validated and quantified using a flow cytometry antibody specific to the human F(ab’)2 fragment (Jackson ImmunoResearch #109-606-006).
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bioRxiv - Cell Biology 2023Quote: ... the blot was washed 3 times with 1× TBST for 15 min each at RT and then incubated with horseradish peroxidase–conjugated goat anti-mouse IgG antibody (1:20000, Jackson ImmunoResearch 115-035-003) diluted in 5% non-fat milk for 1 h at RT ...
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bioRxiv - Immunology 2022Quote: ... The sections were then washed in distilled water followed by wash buffer and incubated with alkaline phosphatase conjugated donkey anti-mouse and horse radish peroxidase conjugated donkey anti-rabbit secondary antibodies (both Jackson ImmunoResearch, West Grove, PA) at 1:100 dilution (prepared in tris buffered saline with 0.5% bovine serum albumin ...
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bioRxiv - Biophysics 2022Quote: ... VCAM-1/Fc chimeric proteins and anti-human Fc antibodies (recombinant human VCAM-1/Fc chimera protein, Fisher 862-VC-100; Fcγ-specific goat anti-human IgG, Jackson Immunoresearch 109-005-008) or poly-L-lysine (Sigma P4707).
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bioRxiv - Microbiology 2022Quote: ... The grids were washed on three drops of PBS before being incubated for 12 min with a 5 nm gold-labelled anti-rabbit secondary antibody (Jackson ImmunoResearch West Grove, PA) at dilutions of 1:10 ...
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bioRxiv - Neuroscience 2022Quote: ... and secondary antibodies were Alexa 488 anti-Rabbit and Alexa 594 anti-Goat (1:500, Jackson Immunoresearch, #111-545-144 and #705-585-147). In tissue from D2-GFP mice ...
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bioRxiv - Molecular Biology 2023Quote: ... Coverslips were washed three times with phosphate-buffered saline (PBS) and then incubated with anti-mouse or anti-rabbit Cy3 conjugated secondary antibodies (Jackson ImmunoResearch, dilution 1:1000) in antibody buffer for one hour at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... The primary antibody was visualized by Alexa Fluor 488 AffiniPure Donkey Anti-Guinea Pig IgG (H+L) (1:1,000, Jackson ImmunoResearch Laboratories, 706-545-148).
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bioRxiv - Neuroscience 2023Quote: ... The slides were washed 3x for 30 min in PBS and incubated in a secondary antibody solution containing 1:500 dilution of anti-rabbit Cy3-conjugated (Jackson ImmunoResearch, 111-165-144) and anti-mouse Cy-5 conjugated (Jackson ImmunoResearch ...
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bioRxiv - Neuroscience 2023Quote: ... slices were washed again in PBS-triton and incubated for 2 hours with the secondary antibodies at 1:1000 dilution (Alexa Fluor 488 anti-chicken (Jackson ImmunoResearch 703-545-155) and Alexa Fluor 568 anti-rabbit (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... The cells were washed 3x with PBS and incubated for 1 h at RT in a secondary antibody solution containing 1:500 dilution of anti-rabbit Cy3-conjugated (Jackson ImmunoResearch, 111-165-144) and anti-mouse Cy-5 conjugated (Jackson ImmunoResearch ...
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bioRxiv - Biochemistry 2022Quote: ... 1:5,000 dilution in PBST) and goat anti-rabbit secondary antibody peroxidase conjugate (1:20,000 dilution in PBST; Jackson ImmunoResearch Laboratories Inc., 111-035-003).
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bioRxiv - Neuroscience 2023Quote: ... Immunostaining was performed by incubating sections in blocking solution (PBS, 10% Donkey Serum, 1% Triton-100, 1h) followed by primary (overnight, 4°C) and secondary antibodies (1-2h, Jackson ImmunoResearch or ThermoFisher Scientific) incubation ...
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bioRxiv - Cell Biology 2022Quote: ... Membranes were rinsed 3 × 5 min with TBS-T and incubated with IRDye goat-anti-mouse or goat-anti-rabbit secondary antibodies (1:10,000 Jackson ImmunoResearch Laboratories, West Grove, PA) diluted in 5% (w/v ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were rinsed with PBS and non-specific antibody binding sites were blocked with 10% normal goat serum (Jackson ImmunoResearch Laboratories, West Grove, PA) diluted in PBS (blocking buffer ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were incubated with the following secondary antibodies at the indicated dilutions: Cy3-conjugated goat anti-mouse (Jackson ImmunoResearch 115-165-003, 1:1000), Cy3-conjugated goat anti-rat (Jackson ImmunoResearch 112-165-003 ...
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bioRxiv - Cell Biology 2023Quote: ... Tissue sections were thoroughly washed and incubated with Alexa Fluor®594 (Ex: 591 nm, Em: 614 nm) conjugated secondary antibody (Jackson ImmunoResearch Lab, Inc.), at a dilution of 1:700 for 1 h at room temperature in dark ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated for 30 min in a Horse Radish Peroxidase conjugated goat anti-guinea pig secondary antibody (1:100; Cat No. 106-035-003, Jackson ImmunoResearch; West Grove, Pennsylvania) diluted in TNB buffer ...
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bioRxiv - Immunology 2023Quote: ... HRP-conjugated anti-mouse IgG (Cat #115-035-146) and anti-mouse IgM (Cat #115-035-075) antibodies were obtained from Jackson Immunoresearch (West Grove, Pennsylvania). Anti-mouse IgM conjugated to Cy3 was from Jackson Immunoresearch (West Grove ...
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bioRxiv - Bioengineering 2023Quote: ... the beads were washed once with ice cold PBSB and incubated with a 1:300 dilution of goat anti-alpaca IgG H+L (also reactive for llama antibodies) Alexa Fluor 647 (Jackson ImmunoResearch, 128-605-160) on ice for 4 min ...
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bioRxiv - Systems Biology 2023Quote: ... Cells were then washed and stained for 1 hour at room temperature with the following secondary antibodies: anti-hamster Cy3 (Jackson ImmunoResearch 127165160, 1:500), anti-rabbit AlexFluor 488 (Invitrogen A11008 ...
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bioRxiv - Developmental Biology 2023Quote: ... The cells were then washed with PBS three times (5 minutes each) and incubated with Alexa Fluor 488 anti-mouse secondary antibody (1:1000, Jackson Immunoresearch #115-545-146) at 4°C overnight ...
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bioRxiv - Bioengineering 2024Quote: ... Next day, the cells on the slides were incubated with secondary antibodies (donkey anti-rabbit, 1:500) (Jackson ImmunoResearch, Cat. No. 711-605-152) conjugated with Cy5 for 2 h at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... washed 3 times in PB 0.1 M, a secondary antibody solution (PB 0.1M, Triton 0.3%, NGS 2%, goat anti-rabbit Cyanine Cy3TM (Jackson Immunoresearch, 111-165-003, RRID: AB_2338000), goat anti-mouse Cy5TM (Jackson Immunoresearch ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were then extensively washed with PBS and incubated for 45 minutes at 37°C in a humidified chamber with the secondary anti-rat IgG Alexa 594 antibody (1:500; Jackson ImmunoResearch, #112-585-167) diluted in 3% BSA in PBS ...
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bioRxiv - Neuroscience 2024Quote: ... The sections were then incubated in the same fresh medium containing biotinylated goat anti-rabbit IgG antibody (1:1,000; Jackson ImmunoResearch, West Grove, PA, USA) for 2 h at room temperature ...
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bioRxiv - Immunology 2024Quote: ... slides/wells were washed in 0.01-M PBS and incubated in the same blocking buffer but with appropriate secondary antibodies (647 donkey anti-guinea pig, and 594 donkey anti-rabbit, Jackson Immunoresearch, West Grove, PA). The gelatin-embedded DRG sections in the wells were then placed carefully on gelatin-coated slides ...
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bioRxiv - Immunology 2024Quote: ... goat anti-mouse secondary detection antibody conjugated to HRP was diluted 1:10,000 for IgG (peroxidase conjugated goat anti-mouse IgG H&L, Jackson ImmunoResearch, cat. 115-035-003) and 1:5,000 for IgA (peroxidase conjugated goat anti-mouse IgA ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated overnight at 4°C in primary antibody solution containing 2% Normal Donkey Serum (NDS; Jackson Immunoresearch Laboratories Inc. 017-000-121) + 1xPBS + 0.03% Triton + primary antibody (rabbit anti-P2RY12 ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were then rinsed with PBS and incubated with Cy3-conjugated goat anti-rabbit secondary antibody (Millipore Sigma, Billerica, MA; Jackson Immunoresearch, Philadelphia, PA) diluted 1:800 in BSA-PBS (1%) ...
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bioRxiv - Neuroscience 2024Quote: ... sections were washed in PBS (3×5 min) and incubated overnight with secondary antibody (peroxidase conjugated donkey-anti-rabbit 1:500, Jackson Immunoresearch, Baltimore, PA, USA). The Tyramide Signal Amplification procedure was then employed ...
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bioRxiv - Neuroscience 2024Quote: ... Slides were then placed in secondary antibodies, Alpha-Bungarotoxin AF555 1:500 (ThermoFischer, #B35451) and Donkey anti-Rabbit AF488 1:450 (Jackson ImmunoResearch, #711-545-152), diluted in PBS1X for 2.5 hours at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were incubated in secondary antibody solution (0.1% Triton 100X in PBS) for 2 hours at room temperature with the following secondary antibodies: Cy3 donkey anti-rabbit IgG (1:1000, Jackson ImmunoResearch, #711-165-152) and Cy5 donkey anti-guinea pig IgG (1:1000 ...
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Anatomical characterisation of somatostatin-expressing neurons belonging to the anterolateral systembioRxiv - Neuroscience 2024Quote: ... and then for 2-18 hours in appropriate species-specific secondary antibodies that were raised in donkey and conjugated to Alexa 488 or Rhodamine Red (from Jackson Immunoresearch, West Grove, PA) or to Alexa 555-plus (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2024Quote: ... sections were washed 4x in PBT and incubated for 90 min in secondary antibody (Alexafluor 488 AffiniPure Donkey Anti-Rabbit, 1:1000, Jackson Immunoresearch # 711-545-152). The secondary antibody was then washed off with PBT in 3X10 min washes ...
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bioRxiv - Neuroscience 2024Quote: ... The brain sections were rinsed for three times with PBS and incubated with the secondary antibody AlexaFluor 488 donkey anti-rabbit IgG (1:500, cat#: 711-545-152, Jackson ImmunoResearch Labs, RRID: AB_2313584) and Cy5-conjugated streptavidin (1:1000 ...
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bioRxiv - Cell Biology 2024Quote: ... Tissue sections were washed with PBS-T and incubated in the dark with the appropriate Alexa Fluor® -594 or -647 conjugated secondary antibody (1:700; Jackson ImmunoResearch Laboratories, Inc.) for one hour at room temperature ...
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bioRxiv - Biophysics 2021Quote: ... Secondary antibody: Alexa Fluor® 488 AffiniPure F(ab’)2 Fragment Donkey Anti-Mouse IgG (H+L) (Jackson ImmunoResearch Laboratories Inc., code. 715-546-151).
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bioRxiv - Cell Biology 2020Quote: ... cells immunostained for E-cadherin with Alexa Fluor 488 secondary antibody were blocked with 26 µg/ml of AffiniPure Fab Fragment Goat Anti-Mouse IgG (H+L) (Jackson ImmunoResearch, Inc, West Grove, PA) in 5% NGS in PBS overnight before being stained for LAMP1 with Alexa Fluor 594-conjugated goat anti-mouse secondary antibody (Invitrogen).
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bioRxiv - Bioengineering 2022Quote: ... The cells were then washed and incubated at 37°C for 60 min with the appropriate secondary antibody (Alexa Fluor 488 donkey anti-rabbit IgG and Alexa Fluor 594 donkey anti-mouse IgG 1:1000; Jackson ImmunoResearch, West Grove, PA, USA) in blocking buffer prior to a final incubation with 4,6-diamidino-2-phenylindole (DAPI ...