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Citations for Jackson ImmunoResearch :
2851 - 2900 of 2920 citations for AKT1 2 3 Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: ... Staining with primary antibodies was performed for 24 h (at 4 °C) with subsequently 2 h incubation with secondary antibodies (Jackson ImmunoResearch, West Grove, PA) diluted with 1 % BSA ...
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bioRxiv - Molecular Biology 2023Quote: ... After washing once in PBS-T (PBS with 0.2% Tween-20), cells were blocked with blocking buffer (PBS-T, 2% BSA, and 5% goat serum (Jackson Immunoresearch/Dianova, 017-000-121) for 1 hour at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... 1×106 cells in 100 μl were stained with APC conjugated anti-human F(ab’)2 fragment specific antibody (Jackson ImmunoResearch, West Grove, PA) at a dilution of 1:100 and incubated for 1 hour at room temperature ...
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bioRxiv - Developmental Biology 2023Quote: ... After washing once in PBS-T (PBS with 0.2% Tween-20), cells were blocked with blocking buffer (PBS-T, 2% BSA and 5% goat serum (Jackson Immunoresearch/Dianova, 017-000-121) for 1 hour at room temperature ...
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bioRxiv - Genomics 2023Quote: ... for 1 hour, followed by incubation with primary antibodies (overnight, 4°C) and secondary antibodies (1-2 hours, from Jackson ImmunoResearch or ThermoFisher Scientific). The slides were then mounted with coverslips using VECTASHIELD (Vector Labs ...
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bioRxiv - Immunology 2023Quote: Raft-APEX2 A20 D1.3 cells were starved for 20 min in serum-free media and incubated with either 10 μg/ml of goat anti-mouse IgM F(ab’)2 fragments (Jackson ImmunoResearch 115-006-020) for 10 min or 0.1 ...
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bioRxiv - Immunology 2023Quote: ... for 45 min and activated with 0 or 10 μg/ml of goat anti-mouse IgM F(ab’)2 fragments (Jackson ImmunoResearch 115-006-020) for 5 ...
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bioRxiv - Bioengineering 2023Quote: ... incubated on ice with a 1:200 dilution of goat anti-mouse AlexaFluor 488 and a 1:1000 dilution of donkey anti-chicken IgY F(ab)’2 Alexa Fluor 647 (Jackson ImmunoResearch, 703-606-155) on ice for 4 min ...
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bioRxiv - Bioengineering 2023Quote: ... The beads were then incubated with a 1:1000 dilution of donkey anti-chicken IgY F(ab)’2 Alexa Fluor 647 (Jackson ImmunoResearch, 703-606-155) on ice for 4 min ...
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bioRxiv - Immunology 2023Quote: ... and then stimulated with soluble or pre-adsorbed plate-bound (both at 10 μg/ml) goat anti-mouse IgM F(ab’)2 fragments (Jackson ImmunoResearch 115-006-020) for 15 min in the presence of TAK981 inhibitor and lysed by addition of 5x SDS lysis buffer to the final concentration of 2% SDS ...
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bioRxiv - Immunology 2023Quote: ... then incubated at 37 °C for 5 min and fixed for processing as above with exception of staining with Atto488-labelled goat anti-mouse IgM F(ab’)2 fragments (Jackson ImmunoResearch 115-006-020) instead of WGA-Atto488 ...
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated overnight at 4°C in primary antibody solution containing 2% Normal Donkey Serum (NDS; Jackson Immunoresearch Laboratories Inc. 017-000-121) + 1xPBS + .03% Triton + primary antibodies (chicken anti-Iba1 ...
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bioRxiv - Cancer Biology 2023Quote: ... CAR expression post-transduction was validated and quantified using a flow cytometry antibody specific to the human F(ab’)2 fragment (Jackson ImmunoResearch #109-606-006).
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Localization of Cadherins in the postnatal cochlear epithelium and their relation to space formationbioRxiv - Cell Biology 2024Quote: Tissues were washed in PBS/0.5% Triton and blocked at for at room temperature (RT) 1 hour with 2% Donkey serum (Jackson ImmunoResearch, West Grove, PA, USA) in PBS/0.5% Triton ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated overnight at 4°C in primary antibody solution containing 2% Normal Donkey Serum (NDS; Jackson Immunoresearch Laboratories Inc. 017-000-121) + 1xPBS + 0.03% Triton + primary antibody (rabbit anti-P2RY12 ...
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bioRxiv - Neuroscience 2020Quote: ... Slides were washed 4X for 5 min each in T-Buffer (TBS containing 0.05% Triton-X100) followed by incubation in Alexa Fluor 647-conjugated donkey anti-rabbit IgG (Jackson ImmunoResearch, West Grove, PA, diluted at 1:200) for 2 h at room temperature in a humidified chamber ...
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bioRxiv - Neuroscience 2019Quote: ... Sections were rinsed three times for 10 minutes in TBS and incubated for 45 minutes with goat Cy3-coupled anti-rabbit (1:500, Jackson ImmunoResearch Labs Cat# 111-165-003, RRID:AB_2338000), goat Alexa Fluor 647-coupled anti-guinea pig (1:500 ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were washed three times for 10 min each in PBS before they were incubated in secondary 488 goat anti-rabbit (1:1000 working dilution; Jackson ImmunoResearch Labs Cat# 111-095-144, RRID:AB_2337978) and 647 goat anti-mouse (1:500 working dilution ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were washed three times for 10 min each in PBS before they were incubated in secondary 594 goat anti-rabbit (1:1000 working dilution; Jackson ImmunoResearch Labs Cat# 111-585-144, RRID:AB_2307325) in PBS and 0.3% Triton X ...
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bioRxiv - Neuroscience 2020Quote: ... tissue was washed 3x with 0.1M PBS and then incubated with a solution containing 1:500 Biotin-SP-conjugated Donkey anti-rabbit secondary antibody (Jackson ImmunoResearch Labs Cat# 711-065-152, RRID:AB_2340593). After washing ...
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bioRxiv - Systems Biology 2022Quote: ... blots were incubated with secondary antibody peroxidase-conjugated affinipure goat anti-mouse lgG and peroxidase-conjugated affinipure goat anti-rabbit lgG (H+L, Jackson ImmunoResearch, 115-035-003, 111-035-144) for 1 h at room temperature ...
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bioRxiv - Cancer Biology 2020Quote: ... slides were incubated with the secondary antibody (biotin-SP-conjugated AffiniPure donkey anti-mouse, anti-rabbit or anti-goat, Jackson ImmunoResearch, all 1:500 in PBS-T) for 40 min ...
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bioRxiv - Neuroscience 2020Quote: ... primary antibodies followed by washing and incubation with fluorescence-labeled donkey anti-rabbit AlexaFluor488 and donkey anti-mouse or goat Cy3 secondary antibodies 1:500 (Jackson ImmunoResearch Laboratories Inc.; West Grove, PA, USA) for 2 hours at RT.
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bioRxiv - Neuroscience 2019Quote: ... followed by PBS washes and incubation for 1 hr with goat anti-rabbit IgG conjugated with Cy5 (1:100; Jackson ImmunoResearch Labs Cat# 111-175-144, RRID:AB_2338013) in PBS with 2% BSA ...
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bioRxiv - Cell Biology 2021Quote: ... The slides were then washed 3 times in PBST for 5 minutes and incubated with TRITC-conjugated goat anti-rabbit (for α-GLH-1) or TRITC-conjugated goat anti-mouse (for K76) (Jackson ImmunoResearch laboratories Inc., West Grove, PA). Slides were incubated for two hours in a dark humidity chamber and then washed 3 times in PBST for 5 minutes ...
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bioRxiv - Microbiology 2022Quote: ... before resuspending in 25 μl of 0.2 µm-filtered PBS containing polyclonal Alexa Fluor 647 Rabbit Anti-Mouse IgG (Jackson ImmunoResearch, 15 µg/ml, 315-605-003, AB_2340239) or monoclonal Brilliant Violet 421 Rat Anti-Mouse IgA (BD Bioscience ...
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bioRxiv - Neuroscience 2022Quote: ... 3x for 5 min and incubated at room temperature with blocking buffer containing goat anti-rabbit HRP secondary antibody (Jackson Immunoresearch labs, #111-035-003, 1:5000). Blots were rinsed in wash buffer 3x for 5 min ...
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bioRxiv - Neuroscience 2022Quote: ... slices were incubated with secondary antibody (goat anti-rabbit Alexa488, Jackson ImmunoResearch, CatLog 111-545-144, 1:2000 dilution; Donkey anti-mouse Cy3, Jackson ImmunoResearch, Lo#125797, 1:2000 dilution). Images were acquired with Nikon A1 confocal microscope equipped with 20x and 4x objectives ...
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bioRxiv - Cell Biology 2024Quote: ... Secondary gold labeling was performed for 1.5 hours at 1:20 dilution in blocking solution (18 nm gold donkey-anti-mouse and donkey-anti-rabbit IgG, Jackson ImmunoResearch 705-215-147, 711-215-152). Post-staining ...
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bioRxiv - Microbiology 2023Quote: ... the samples were incubated for 45 min at room temperature with a goat anti-mouse antibody conjugated to 6 nm colloidal gold particles and a goat anti-rabbit antibody conjugated with 12 nm colloidal gold particles (Jackson ImmunoResearch Laboratories, Inc. West Grove, PA, USA). After incubation ...
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bioRxiv - Immunology 2023Quote: ... used 1.5 x 106 cells that were blocked with 2.24 μg/ml of guinea pig and rabbit immunoglobulin (Jackson Immunoresearch, Cat#: 011-000-003 and 006-000-002) in FACS buffer (PBS containing 1% FBS and 0.01% sodium azide ...
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bioRxiv - Neuroscience 2023Quote: ... incubated with a biotinylated secondary antibody (biotinylated donkey anti-rabbit, Jackson ImmunoResearch, 711-065-152, 1:500; or biotinylated donkey anti-goat, Jackson ImmunoResearch, 705-065-147, 1:500) for 1 hour at RT ...
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bioRxiv - Biophysics 2024Quote: ... rabbit anti-CENP-C polyclonal antibody (Bassett et al., 2010) (1 μg/mL) and cy3-conjugated Goat anti-Rabbit IgG (Jackson ImmunoResearch Laboratories #111-165-144, 1:200), for CENP-T immunofluorescence ...
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bioRxiv - Microbiology 2024Quote: ... HRP conjugated secondary antibodies (donkey α rabbit for α HTNV, Jackson Immunoresearch 711-035-152; or goat α mouse for α SEOV, Jackson Immunoresearch 115-035-003) were incubated for 2 hours at room temperature ...
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bioRxiv - Biophysics 2024Quote: ... rabbit anti-CENP-C polyclonal antibody (Bassett et al., 2010) (1 μg/mL) and cy3-conjugated Goat anti-Rabbit IgG (Jackson ImmunoResearch Laboratories #111-165-144; 1:200), for Ndc80/Hec1 ...
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bioRxiv - Microbiology 2024Quote: ... Lung lobes were then transferred to secondary antibody solution (1:50 Alexa Fluor 790 AffiniPure Fab Fragment Goat anti-rabbit, Jackson Immunoresearch Laboratories, Cat no: 111-657-008), in blocking buffer and incubated for a further 2 days at 37°C with gentle shaking for in the dark ...
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bioRxiv - Biophysics 2021Quote: ... Secondary antibody: Alexa Fluor® 488 AffiniPure F(ab’)2 Fragment Donkey Anti-Mouse IgG (H+L) (Jackson ImmunoResearch Laboratories Inc., code. 715-546-151).
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bioRxiv - Cell Biology 2019Quote: ... As secondary antibody for flow cytometry we used PE-conjugated goat anti-human IgG + IgM (H+L) (F(ab’)2) (Jackson Immunoresearch, no. 109-116-127).
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bioRxiv - Neuroscience 2020Quote: ... slices were washed three times with PBS (pH 7.4) and then incubated for 2 h at room temperature with Alexa 488 goat anti mouse 1:1000 (Jackson ImmunoResearch, cat n° 115-545-166), Alexa 594 goat anti rabbit 1.1000 (Jackson ImmunoResearch ...
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bioRxiv - Immunology 2020Quote: ... BCR-mediated Ca2+ mobilization was measured for 60s after the cells were stimulated either with 10 μg/mL F(ab′)2 of polyclonal goat anti-mouse IgM (Jackson ImmunoResearch, West Grove, PA, USA) or 0.5mM phosphatidylcholine (PtC ...
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bioRxiv - Bioengineering 2021Quote: ... Approximately 200 RUs of VHH-Fcs (2 µg/mL) were captured on goat anti-human IgG surfaces (4000 RUs, Jackson ImmunoResearch, Cat#109-005-098) at a flow rate of 10 µL/min for 30 s ...
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bioRxiv - Neuroscience 2022Quote: ... tissues were incubated for 2 hours at room temperature in Alexafluor-488 conjugated donkey anti-chicken (1:500; Jackson ImmunoResearch 703-546-155, lot# 142461) in 1% goat serum and 0.3% Triton X100 in PBS ...
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bioRxiv - Neuroscience 2021Quote: ... sections were incubated for 2 h at room temperature with a secondary antibody conjugated to 6-nm gold particles (Jackson ImmunoResearch, cat # 115-195-146) diluted to 1:40 in blocking buffer ...
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bioRxiv - Immunology 2020Quote: ... 50 μL of red-phycoerythrin (R-PE) conjugated F(ab’)2 fragment goat anti-mouse IgG specific to the Fc fragment (Jackson ImmunoResearch, Inc., West Grove, PA) were added at 1 μg/ml to the wells and incubated for 45 minutes ...
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bioRxiv - Immunology 2021Quote: ... Antibody binding was detected using goat-anti-human IgG-Alexa fluor-488 or anti-human IgG F(ab’)2-Alexa fluor-488 (Jackson ImmunoResearch, West Grove, PA, USA), and mean cellular fluorescence was determined using flow cytometry (Intellicyt iQue ...
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bioRxiv - Immunology 2022Quote: ... Plates were washed and the SARS-CoV-2 RBD specific IgG signal was detected by incubating with horseradish peroxidase (HRP) conjugated - anti-human IgG (Jackson ImmunoResearch Labs, #109-036-098). Plates were then washed thoroughly and developed with o-phenylenediamine (OPD ...
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bioRxiv - Microbiology 2023Quote: ... We then washed cells three times in binding buffer and incubated them with a R-phycoerythrin (PE)-coupled goat anti-human F(ab’)2 fragment (Jackson ImmunoResearch Cat #: 109-116-098) in binding buffer for 30 minutes at 4 °C ...
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bioRxiv - Microbiology 2023Quote: ... Staining of wild-type and mutant EEEV or SFV E3–E2–(6K/TF)–E1 was detected with a PE-conjugated donkey anti-mouse F(ab’)2 fragment (Jackson ImmunoResearch Cat#: 715-116-150) using flow cytometry ...
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bioRxiv - Neuroscience 2023Quote: ... donkey anti-goat or anti-mouse biotinylated secondary antibodies were incubated for 2 hours at room temperature (1:1000, Jackson ImmunoResearch, West Grove, PA, USA), followed by incubation with VECTASTAIN Elite ABC HRP solution (Vector ...
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bioRxiv - Immunology 2023Quote: ... or 10 μg/ml AF-488/−647 labelled F(ab’)2 Fragments of donkey anti-mouse IgM (Jackson ImmunoResearch 715-546-020/715-606-020), were placed in 300 μl of imaging buffer (10% FCS in PBS ...