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Citations for Jackson ImmunoResearch :
2751 - 2800 of 2920 citations for AKT1 2 3 Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... the cells were incubated with 4’,6-diamidino-2-phenylindol (DAPI; 1 μg/ml) and the corresponding Alexa Fluor-conjugated secondary antibodies (1:500; Jackson ImmunoResearch Laboratories) for 2 h at RT ...
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bioRxiv - Cancer Biology 2022Quote: ... anti-rabbit Rhodamine-Red-X conjugated and anti-mouse Alexa Fluor® 647 conjugated fluorophore-labeled F(ab)2 donkey secondary antibodies were used (Jackson Immunoresearch). Sections were DAPI counterstained and 3×3 Z-stacked large images (9-5 mm depth ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were subsequently stained with F(ab’)2 fragments conjugated with organic fluorophores: AlexaFluor 647 (Thermo Fischer Scientific A37573) anti-mouse IgG (donkey polyclonal, 1:50; Jackson Immunoresearch AB_2340761), and CF680 (Biotium 92139 ...
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bioRxiv - Microbiology 2023Quote: ... Cells were washed twice and incubated with Allophycocyanin (APC)-conjugated F(ab′)2 fragment donkey anti-human IgG antibody (1:100 dilution; catalog number 709-136-149; Jackson ImmunoResearch Laboratories) for 30 minutes ...
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bioRxiv - Bioengineering 2022Quote: ... The cells were then washed and incubated at 37°C for 60 min with the appropriate secondary antibody (Alexa Fluor 488 donkey anti-rabbit IgG and Alexa Fluor 594 donkey anti-mouse IgG 1:1000; Jackson ImmunoResearch, West Grove, PA, USA) in blocking buffer prior to a final incubation with 4,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Neuroscience 2020Quote: ... Slides were washed 3X for 10 min each in T-Buffer followed by one wash in TBS and incubation in Alexa Fluor 647-conjugated donkey anti-rabbit IgG (Jackson ImmunoResearch, diluted at 1:250) and Alexa Fluor 488-conjugated donkey anti-rat IgG (Jackson ImmunoResearch ...
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bioRxiv - Immunology 2021Quote: ... slides were washed and incubated for 1 h at room temperature with anti-rabbit horseradish peroxidase-conjugated antibodies (Jackson ImmunoResearch #111-035-003, 1:500). Peroxidase activity was detected using 3,3’ Diaminobenzidine substrate (BD Pharmingen #550880) ...
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bioRxiv - Molecular Biology 2019Quote: ... Secondary antibodies used were horseradish peroxidase-conjugated anti-mouse or anti-rabbit (115-035-174 and 211-032-171, Jackson ImmunoResearch Laboratories; both 1:10,000).
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bioRxiv - Neuroscience 2019Quote: ... and rhodamine red -X-coniugated affinity pure goat anti-rabbit IgG (H+L) (1: 50 dilution; 111-295-003; Jackson ImmunoResearch Europe Ltd, Cambridge, UK) for 1 h at room temperature ...
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bioRxiv - Physiology 2020Quote: ... The secondary antibodies were peroxidase-conjugated AnffiniPure Goat Anti-Rabbit or mouse IgG H&L (Alexa Fluor® 488; Jackson ImmunoResearch Laboratories, West Grove, PA) at 1:500 dilution ...
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bioRxiv - Physiology 2019Quote: ... The antibody-antigen complexes were visualized by incubation for 1 hour at room temperature with 1:2000 Alexa Fluor 488-conjugated goat anti-rabbit (Molecular Probes A11008/Jackson ImmunoResearch, 111-485-144) and Cy5-conjugated donkey anti-mouse (Jackson ImmunoResearch ...
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bioRxiv - Neuroscience 2019Quote: ... sections were rinsed again before being incubated for 1h in a biotinylated donkey anti-rabbit secondary antibody (Jackson Immunoresearch, West Grove, PA, diluted 1: 500) in 0.1M PBS + 0.4% TX + 1% NDS ...
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bioRxiv - Molecular Biology 2019Quote: ... antibody and detected with 1:200 anti-rabbit CY3 (Jackson Immunoreserach, cat# 715-165-151) or 1:200 Alexa 488 Mouse (1:200, Jackson Immunoresearch, cat# 715-545-150) secondary antibodies ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then incubated in secondary donkey anti-rabbit Alexa 594 or donkey anti-chicken Alexa 488 (1:500, Jackson ImmunoResearch Laboratories, West Grove, PA) in blocking buffer overnight at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... blots were incubated 1 h at room temperature with goat anti-rabbit (1:10000) or anti-mouse (1:5000) HRP-conjugated secondary antibodies (Jackson ImmunoResearch Europe Ltd, Newmarket, UK) and signal was detected with WesternBright ECL Spray (Advansta ...
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bioRxiv - Bioengineering 2021Quote: ... Cells were then incubated at 37°C for 60 min with a secondary antibody (Alexa Fluor 594 donkey anti-rabbit IgG and Alexa Fluor 594 donkey anti-mouse IgG, 1:1000; Jackson ImmunoResearch, West Grove, PA, USA) in blocking buffer before final incubation with 4,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Cell Biology 2020Quote: ... Immunoreactivity was detected using the following secondary peroxidase conjugated antibodies: goat anti-rabbit and anti-mouse IgG-HRP (111-035-003, 115-035-003; Jackson ImmunoResearch Laboratories, Inc. Cambridge, UK) and enhanced chemiluminescent (ECL ...
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bioRxiv - Neuroscience 2022Quote: ... The sections were then processed for double immunofluorescence by using in combination anti-Homer1bc and anti-PSD-95 primary antibodies and the appropriate fluorescent secondary antibodies (anti-mouse or anti-rabbit 1:1000; Jackson ImmunoResearch, West Grove, PA, USA) following the same protocol used for mouse brain tissue (see Pizzo et al. ...
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bioRxiv - Neuroscience 2021Quote: ... Primary antibody incubation was followed by washes and incubation with secondary antibody for 1h (HRP-conjugated goat anti-rabbit IgG, and HRP-conjugated goat anti-mouse; Jackson ImmunoResearch Laboratories, West Grove, PA). Protein bands were visualized using the ECL detection system (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... Slides were washed three times with TBS buffer with 0,05 % Tween-20 and further incubated with anti-rabbit Alexa 647 (Jackson ImmunoResearch Cat. No. 111-606-144), anti-human IgG-Alexa647 (Southern Biotech ...
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bioRxiv - Microbiology 2021Quote: ... overnight at 4°C and then incubated with anti-mouse or rabbit IgG-HRP conjugate (209-035-082 or 309-035-003, Jackson ImmunoResearch, West Grove, PA, USA) for 1 h at 37 °C ...
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bioRxiv - Neuroscience 2020Quote: ... sections were rinsed in PBS and then incubated for 1 hr in the appropriate biotinylated secondary antibody (anti-mouse or anti rabbit, 1:600 Jackson ImmunoResearch, West Grove, PA, USA) in PBS with 0.4% Triton-X 100 ...
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bioRxiv - Neuroscience 2019Quote: ... Bound primary antibodies were visualized by incubation with peroxidase-conjugated anti-mouse or anti-rabbit secondary antibody (1:10000; Jackson ImmunoResearch Laboratories, West Grove, PA) followed by treatment with the ECL-plus reagent (Western Lighting ...
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bioRxiv - Bioengineering 2021Quote: ... The channels were incubated at 25 °C for 60 min with the corresponding secondary antibodies AlexaFluor 594 donkey anti-rabbit IgG (1:1000; Jackson ImmunoResearch, West Grove, PA, USA) and Alexa Fluor 648 donkey anti-rat IgG (1:1000 ...
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bioRxiv - Neuroscience 2019Quote: ... 0.2% TX and a biotinylated goat anti-rabbit secondary antibody conjugated with fluorescein (FITC) for PGR (1:200, Jackson ImmunoResearch Laboratories Inc., West Grove, PA), and a rhodamine-conjugated goat anti-mouse secondary antibody (TRITC ...
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bioRxiv - Cell Biology 2019Quote: ... Alexa Fluor 594-conjugated Donkey Anti-Mouse IgG (1:250) and FITC-conjugated Goat Anti-Rabbit IgG (1:200; both Jackson ImmunoResearch Laboratories, West Grove, PA). Texas-Red conjugated Goat Anti-Mouse IgG (1:250 ...
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bioRxiv - Neuroscience 2022Quote: ... sections were rinsed in five times in PBS and then incubated for 1 hr in biotinylated secondary antibody (anti rabbit, 1:600 Jackson Immunoresearch, West Grove, PA, USA) in PBS with 0.4% Triton-X 100 ...
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bioRxiv - Neuroscience 2022Quote: ... Cat#ACC-102) over night at 4°C followed by 1 hour probing with donkey anti-rabbit HRP (1:10,000; Jackson ImmunoResearch Labs Cat#711-035-152). The membrane were imaged via enhanced chemiluminescence photodetection with the BioRad ChemiDoc XRS+ system.
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bioRxiv - Neuroscience 2022Quote: ... sections were rinsed in PBS and then incubated for 1 hr in a biotinylated secondary antibody (anti-rabbit, 1:600 Jackson ImmunoResearch, West Grove, PA, USA) in PBS with 0.4% Triton-X 100 ...
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Formation of a giant unilocular vacuole via macropinocytosis-like process confers anoikis resistancebioRxiv - Cell Biology 2024Quote: ... The membrane was washed three times with PBST and then incubated for 1 h at room temperature with Peroxidase AffiniPure Goat Anti-Rabbit IgG (H+L) (Jackson ImmunoResearch Laboratories, 113-035-003) or Goat Anti-Mouse IgG (H+L ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were then washed three times and incubated for 2 h at room temperature with fluorescent secondary antibodies: Cy3 goat anti-rabbit (1/200) and/or AlexaFluor 488 goat anti-mouse (1/200; both from Jackson ImmunoResearch, West Grove, PA, USA). No signal was detected in control sections incubated in the absence of the primary antibody ...
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bioRxiv - Neuroscience 2024Quote: ... and then sections were washed three times in PBS and incubated with AlexaFluor 594 donkey anti-rabbit IgG (Jackson immunoresearch, 711-585-152, 1:500), AlexaFluor 488 donkey anti-goat IgG (Invitrogen ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... HRP-conjugated anti-rabbit IgG (1:5000, NA934V, Cytiva, Tokyo, Japan) or HRP-conjugated anti-mouse IgG (1:10000, 115-035-003, Jackson Immunoresearch, West Grove, PA, USA). Note that STAT6 was immunoblotted after stripping the pSTAT6 signal by using WB Stripping Solution Strong (Nacalai Tesque ...
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bioRxiv - Immunology 2024Quote: ... Cells were then incubated with 0.3 µg of biotin-blocked PerCP-labeled streptavidin and 0.3 µg of APC-Cy7-labeled streptavidin complexed with Bio-pVIII peptide along with a 1:25-fold diluted FITC-labeled goat anti-rabbit IgG-Fc polyclonal antibody (Jackson ImmunoResearch, West Grove, PA, USA) in a final volume of 100 µl for 15 minutes at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... diluted 1:500 and Alexa Fluor 594® donkey anti-rabbit IgG H&L (Cat. No. 711-585-152; Jackson ImmunoResearch, UK; diluted 1:500). After incubation ...
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bioRxiv - Immunology 2023Quote: ... Secondary antibodies labelled with horseradish peroxidase (HRP; anti-rabbit #111-035-144 and anti-mouse #115-035-174) were purchased from Jackson ImmunoResearch (West Grove, PA, USA) and Trans-Blot® Turbo™ Transfer RTA kit from Bio-Rad (Hercules ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were subsequently incubated at room temperature with Alexa 594-conjugated goat anti-rabbit IgGs (1/500; 90 min; Jackson Immunoresearch Laboratories, West Grove, PA). Images were acquired using a confocal microscope (LSM880 ...
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bioRxiv - Neuroscience 2023Quote: ... secondary biotinylated antibody in carrier solution was applied for 1 h (donkey anti-rabbit, 1:500; Jackson ImmunoResearch, #711-065-152; donkey anti-rat, 1:500; Jackson ImmunoResearch, #711-065-153). After incubation with avidin/biotin solution (Vectastain Elite ABC HRP Kit ...
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bioRxiv - Neuroscience 2023Quote: ... then rinsed and incubated for 1 hour at RT in biotinylated donkey anti-rabbit antibody (1:250, 711-065-152, Jackson Immunoresearch Laboratories, West Grove, PA) in PBS ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were rinsed 3 × 5 min with PBS and incubated with AlexaFluor-labeled secondary goat-anti-mouse or goat-anti-rabbit antibodies (1:350, Jackson ImmunoResearch Laboratories, West Grove, PA) diluted in blocking buffer for 2 h at RT ...
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bioRxiv - Immunology 2023Quote: ... and secondaries antibodies Rhodamine RED-X (RRX) rabbit anti-mouse IgM and Alexa Fluor 647 goat anti-mouse IgG (both 10 μg/mL, Jackson ImmunoResearch, West Grove, PA, USA) for 1 hour at RT ...
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bioRxiv - Immunology 2023Quote: ... and secondary antibodies Rhodamine RED-X (RRX) rabbit anti-mouse IgM and Alexa Fluor 647 goat anti-mouse IgG (both 10 μg/mL, Jackson ImmunoResearch, West Grove, PA, USA) for 1 hour at RT ...
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bioRxiv - Immunology 2023Quote: ... The slides were washed three times and incubated with Alexa Fluor 488 conjugated donkey anti-rabbit (1:300, Jackson ImmunoResearch Lab, Cat# 711-545-152), Cy3 conjugated donkey anti-mouse secondary antibody (1:300 ...
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bioRxiv - Plant Biology 2024Quote: PIN1 localization in embryos transgenic for 35Sp:EGFP-ENP used PIN1 primary rabbit antibody incubation (1:1000; 4h, 37°C) and secondary rabbit Cy3-Antibodies (BSA/PBS for 3.5h at 37°C; Jackson ImmunoResearch/USA supplied by Dianova/Hamburg). After repeated washes with PBS and H20 the embryos were embedded in Citifluor antifadent mounting medium and covered with a coverslip ...
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bioRxiv - Neuroscience 2023Quote: ... Some blots were also incubated with an Alexa Fluor® 647-conjugated donkey anti-rabbit IgG (Jackson ImmunoResearch,1:1000, Cat No. 711-605-152) or with Alexa Fluor® 647-conjugated donkey anti-guinea pig (Jackson ImmunoResearch,1:1000 ...
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bioRxiv - Neuroscience 2023Quote: ... slices were washed and incubated with the secondary antibodies (1:500, Donkey Anti-Rabbit conjugated with Alexa Fluor 657, and Donkey Anti-Chicken conjugated with Alexa Fluor 488, Jackson ImmunoResearch Labs, West Grove, PA). Images were acquired using the Biotek Cytation 5 Cell Imaging Multi-Mode Reader (Biotek Instruments ...
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bioRxiv - Bioengineering 2023Quote: ... embryos were washed 3 times (30 min intervals between washes) in the staining buffer and labeled using donkey-anti-rabbit-biotin secondary antibody (Jackson ImmunoResearch, Cat# 711-067-003) in the staining buffer at 4°C overnight at 1 μg/mL ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were then incubated at 37°C for 60 min with a secondary antibody (Alexa Fluor 488 donkey anti-rabbit IgG and Alexa Fluor 594 donkey anti-mouse IgG 1:1000; Jackson ImmunoResearch, West Grove, PA, USA) in blocking buffer prior to a final incubation with 4′,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Neuroscience 2023Quote: ... tissue was washed 4×10 minutes in PBS before being incubated for two hours in secondary antibody diluted in PBS (at 1:200) [Donkey anti-rabbit Cy2 (Jackson Immunoresearch, Cat#711-225-152)] ...
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bioRxiv - Neuroscience 2023Quote: ... and incubated for one hour in a biotinylated secondary antibody (1:600, Biotin-SP AffiniPure Goat Anti-Rabbit IgG, Jackson Immunoresearch Labs; 111-065-003) in 0.4% Triton-X 100 in PBS ...