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Citations for Jackson ImmunoResearch :
201 - 250 of 4631 citations for 3 Hydrazino 5 methyl 4H 1 2 4 triazol 4 ylamine hydrochloride since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... Isolated cells were then cultured in vitro in the presence or absence of 5 mg/ml anti-IgM F(ab)2 (Jackson Immunoresearch) and/or 5 mg/ml anti-CD40 (BioXCell ...
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bioRxiv - Neuroscience 2020Quote: ... the tissue was washed (5 × 5 min) and incubated in Cy3-conjugated donkey anti-mouse (1:500 in PBS; Jackson Immunoresearch, West Grove, PA) for 30 min ...
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bioRxiv - Immunology 2024Quote: ... excess primary antibodies were washed off with PBS (3 washes, 5 minutes each) followed by incubation of donkey anti-mouse Cy3 secondary antibody (Jackson Immunoresearch, Cat# 715-167-003) for 1 h at RT ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were blocked for 1 h in 5% normal donkey serum (Jackson ImmunoResearch Laboratories) in PBS and incubated overnight at 4°C in primary antibody diluted in 1% normal donkey serum in PBS ...
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bioRxiv - Neuroscience 2020Quote: ... Cy™ 5-conjugated donkey anti-mouse (1:500; 715-006-151, Jackson ImmunoResearch), Alexa Fluor® 488-conjugated donkey anti-rat cross-adsorbed against mouse (1:100 ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were blocked for 1 h in 5% normal donkey serum (Jackson ImmunoResearch Inc.) in PBS and incubated overnight at 4°C in primary antibody diluted in 1% normal donkey serum in PBS ...
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bioRxiv - Neuroscience 2023Quote: ... and blocked for at least 1 hour in 5% normal donkey serum (Jackson Immunoresearch) at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... Blocking was performed for 1 hour using 5% donkey serum (DKS; Jackson ImmunoResearch, UK) for the ARX/NKX6.1 staining or 10% fetal calf serum (FCS ...
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bioRxiv - Cell Biology 2020Quote: ... Blotted proteins were incubated with corresponding primary antibodies at 4°C overnight followed by incubation with horseradish peroxidase conjugated secondary antibodies (Jackson ImmunoResearch, West Grove, PA, USA). Amersham ECL Western blotting detection reagent (GE Healthcare Life Sciences ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were incubated overnight at 4°C with the primary antibodies and then with the corresponding horseradish peroxidase-conjugated secondary antibodies (Jackson Immunoresearch, West Grove, PA, USA). All membranes were revealed using ECL chemiluminescence kit according to the manufacturer’s instructions (GE Healthcare ...
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bioRxiv - Neuroscience 2022Quote: ... followed by fluorescently conjugated secondaries in 1:2 primary:secondary molar ratios (Jackson ImmunoResearch). After active labeling ...
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bioRxiv - Neuroscience 2022Quote: ... Cy™3 AffiniPure goat anti-rabbit IgG (H+L) (1:500, Jackson ImmunoResearch, 111-165-003). Sections were washed 3x for 10 minutes per wash on the third day ...
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bioRxiv - Molecular Biology 2021Quote: ... and then goat anti-mouse IgG-HRP (Jackson ImmunoResearch, 1:7,000 dilution in PBS with 3% BSA) was added to the wells and incubated for 1 h at RT ...
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bioRxiv - Developmental Biology 2022Quote: ... we used Cy™3 AffiniPure Donkey Anti-Mouse IgG (H+L) (1:250, Jackson ImmunoResearch, AB_2340813), Alexa Fluor® 488 AffiniPure Donkey Anti-Chicken IgY (IgG ...
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bioRxiv - Neuroscience 2024Quote: ... cyanine 3 (Cy3)-conjugated anti-guinea pig (1:400; Jackson ImmunoResearch, West Grove, PA-706-165-148), Alexa 647-conjugated anti-mouse (1:400 ...
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bioRxiv - Cell Biology 2023Quote: ... Cover slips were then blocked in 150 μL of blocking solution (5% [w/v] BSA, 5% [v/v] Normal Goat Serum [Jackson ImmunoResearch Laboratories] in 1× PBS) at RT for 90 min ...
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bioRxiv - Developmental Biology 2023Quote: ... + 3% donkey serum (Jackson ImmunoResearch)] at room temperature for 15 mins ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and incubated for 2 hr with F(ab)2 donkey anti-rat IgG-Cy3 antibody (1:200; REF-712-166-153, Jackson ImmunoResearch Laboratories, USA). Embryos were washed in TBS stained with 4’,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Microbiology 2021Quote: ... samples were diluted at 1:200 and donkey F(ab’)2 anti-human IgM Fc5μ at 1:400 dilution (1 mg/ml, Jackson ImmunoResearch Laboratories, Inc.) was used as the secondary antibody for detection.
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bioRxiv - Neuroscience 2022Quote: ... Pre-prepared immunopanning dishes were setup as follows: a 10cm petri dish was incubated at 4°C overnight with 30ul anti-rat antibody (Jackson ImmunoResearch Laboratories, Inc, 112-005-167) in 10 ml 50 mM Tris-HCL (pH 9.5) ...
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bioRxiv - Cell Biology 2023Quote: ... cells were fixed with 4% paraformaldehyde and immunofluorescence was performed as described previously [54] using appropriate primary and secondary antibodies (Jackson ImmunoResearch Laboratories, West Grove, PA, USA), and coverslips were mounted using Prolong Gold Antifade Reagent with 4′,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Neuroscience 2023Quote: ... blocked for 1 hour in 5% Normal donkey serum (NDS, Jackson ImmunoResearch Laboratories, Lot# 161590) and permeabilized using 0.5% Triton x-100 in 1X TBS then incubated in primary antibody solution (with corresponding dilutions of each antibody in 1X TBS with 0.5% Triton x-100 ...
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bioRxiv - Microbiology 2024Quote: ... followed by cyanine-5-conjugated donkey-anti-rabbit-IgG secondary Ab (Jackson Immunoresearch; 1:200). All Abs were diluted in phosphate-buffered saline (PBS) ...
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bioRxiv - Developmental Biology 2021Quote: ... The membranes were blocked with 3% nonfat milk and 3% bovine serum albumin (Jackson ImmunoResearch) in Tris-Buffered Saline Tween-20 and probed with antibodies against the cytoplasmic domain of NOTCH4 [23] and β-ACTIN (Abclonal) ...
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bioRxiv - Molecular Biology 2021Quote: ... washed again and blocked in 3% fetal bovine serum/3% normal goat serum (Jackson ImmunoResearch). Primary antibody (CD44 ...
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bioRxiv - Cell Biology 2021Quote: ... washed three times for 5 minutes in TBST and detected with HRP-conjugated secondaries (Jackson Immunoresearch, diluted 1:50,000-1:100,000) after incubation for 1 h at room temperature in 5% non-fat milk in TBST ...
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bioRxiv - Molecular Biology 2020Quote: ... and Cyanine Cy™3-conjugated donkey-anti-rat secondary antibody (1:300; Jackson ImmunoResearch; 712-166-150) at room temperature ...
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Pathogenic variants of sphingomyelin synthase SMS2 disrupt lipid landscapes in the secretory pathwaybioRxiv - Cell Biology 2022Quote: ... Cyanine Cy™3-conjugated donkey anti-mouse IgG (Jackson ImmunoResearch Laboratories, 715-165-150; IF 1:400); Cyanine Cy™3-conjugated donkey anti-goat IgG (Jackson ImmunoResearch Laboratories ...
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Pathogenic variants of sphingomyelin synthase SMS2 disrupt lipid landscapes in the secretory pathwaybioRxiv - Cell Biology 2022Quote: ... Cyanine Cy™3-conjugated donkey anti-rabbit IgG (Jackson ImmunoResearch Laboratories, 715-165-152; IF 1:400); Cyanine Cy™3-conjugated donkey anti-mouse IgG (Jackson ImmunoResearch Laboratories ...
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Pathogenic variants of sphingomyelin synthase SMS2 disrupt lipid landscapes in the secretory pathwaybioRxiv - Cell Biology 2022Quote: ... Cyanine Cy™3-conjugated donkey anti-goat IgG (Jackson ImmunoResearch Laboratories, 705-165-147; IF 1:400), Cyanine Cy™5-conjugated donkey anti-rabbit IgG (Jackson ImmunoResearch Laboratories ...
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bioRxiv - Cell Biology 2020Quote: ... Cryosections were washed 3 times and incubation with the secondary antibody Cy3-anti-rabbit (1:250, Jackson ImmunoResearch) and counterstaining with WGA was performed for 2 h at room temperature ...
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bioRxiv - Bioengineering 2023Quote: ... cells were incubated with 3 µg/mL AffiniPure Goat x Rabbit (1:100, Jackson ImmunoResearch; 111-005-144), Goat x Mouse (1:100 ...
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bioRxiv - Systems Biology 2024Quote: ... Cy™3 AffiniPure™ Goat Anti-Mouse IgG (H+L) (Jackson ImmunoResearch 115-165-003, 1:500). Quantification of calcium signal was performed using ImageJ software on z-stacks of imaged sections ...
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bioRxiv - Immunology 2021Quote: ... anti-IgM F(ab’)2 (1 ug/ml, Jackson Immunoresearch, Cat#115-006-020), IL-21 (50 ng/ml ...
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bioRxiv - Neuroscience 2022Quote: ... followed by fluorescently conjugated secondaries in 1:2 primary : secondary molar ratios (Jackson ImmunoResearch). After active labeling ...
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bioRxiv - Neuroscience 2020Quote: ... then with anti-mouse horseradish peroxidase-conjugated antibodies (1/3000; 2 h; Jackson ImmunoResearch) and revealed with ECL substrate (Lumi-Light Western Blotting Substrate ...
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bioRxiv - Immunology 2022Quote: ... resuspended in 20µL of FACS buffer containing 1-2% normal mouse serum (Jackson ImmunoResearch) and 1-2% mouse gamma globulin ...
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bioRxiv - Immunology 2023Quote: ... serum and 1% AffiniPure F(ab′)2 fragment donkey anti-mouse IgG (Jackson ImmunoResearch). Primary antibodies were diluted in IF buffer at the dilutions detailed below (Supplementary table 5 ...
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bioRxiv - Immunology 2024Quote: ... 1:1000 diluted Alkaline phosphatase conjugated anti-human F(ab’)2 antibodies (Jackson ImmunoResearch) were incubated in the plate for 1 hr and then washed ...
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bioRxiv - Neuroscience 2023Quote: ... followed by fluorescently conjugated secondary antibodies in 1:2 (primary:secondary) molar ratios (Jackson ImmunoResearch). After active labeling ...
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bioRxiv - Neuroscience 2024Quote: ... 2% v/v normal goat serum (Jackson ImmunoResearch, 005-000-121, 1:500 dilution), and 0.1% w/v Triton X-100 in 1×PBS at 4 °C for 24 hours ...
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bioRxiv - Neuroscience 2024Quote: ... and then by fluorescently conjugated secondaries in 1:2 primary:secondary molar ratios (Jackson ImmunoResearch). Following active labeling ...
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bioRxiv - Neuroscience 2024Quote: ... followed by fluorescently conjugated secondaries in 1:2 primary: secondary molar ratio (Jackson ImmunoResearch). After active labeling ...
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bioRxiv - Cell Biology 2023Quote: ... samples were then transferred directly into blocking buffer (4% BSA (Sigma Cat# 10735086001)/3% goat serum (Jackson Immunoresearch #005000121)/PBS) ...
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bioRxiv - Developmental Biology 2021Quote: ... and blocked for 1 hr with 5% normal donkey serum (Jackson ImmunoResearch Laboratories #017-000-121) diluted in 1× PBST (blocking solution) ...
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bioRxiv - Biochemistry 2022Quote: ... in PBS at 3 times and were incubated with Cy3-conjugated mouse anti-rabbit IgG (1:2,000; Jackson ImmunoResearch) in 0.5% BSA in PBS for 30 min at 37°C as a secondary antibody ...
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bioRxiv - Cancer Biology 2024Quote: ... detected with FITC-conjugated AffiniPure Donkey Anti-Rabbit IgG (1:1000 dilution in 3% BSA in PBS; Jackson ImmunoResearch), and counterstained with DAPI ...
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bioRxiv - Genomics 2021Quote: ... 5% normal donkey serum and 5% normal goat serum (Jackson Immunoresearch) for 30 min at room temperature ...
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bioRxiv - Developmental Biology 2023Quote: ... After washing once in PBS-T (PBS with 0.2% Tween-20), cells were blocked with blocking buffer (PBS-T, 2% BSA and 5% goat serum (Jackson Immunoresearch/Dianova, 017-000-121) for 1 hour at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... After washing once in PBS-T (PBS with 0.2% Tween-20), cells were blocked with blocking buffer (PBS-T, 2% BSA, and 5% goat serum (Jackson Immunoresearch/Dianova, 017-000-121) for 1 hour at room temperature ...