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Citations for Jackson ImmunoResearch :
151 - 200 of 4631 citations for 3 Hydrazino 5 methyl 4H 1 2 4 triazol 4 ylamine hydrochloride since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2022Quote: Brain sections were washed with PBS and then placed in blocking solution (PBS containing 0.1% TritonX-100, 4% normal goat serum, Jackson Immunoresearch) for 1 hr at room temperature ...
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bioRxiv - Pathology 2023Quote: ... Overnight incubation at 4°C with primary antibodies were followed by incubation with fluorophore-coupled anti-species antibodies (Jackson Immunoresearch) for one hour at room temperature.
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bioRxiv - Cell Biology 2023Quote: ... was performed overnight at 4 °C while nutating: donkey anti-rabbit Alexa Fluor 488 (Jackson ImmunoResearch #111-545-144; RRID:AB_2338052), donkey anti-goat Cy2 (Jackson ImmunoResearch #705-225-147 ...
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bioRxiv - Neuroscience 2021Quote: ... goat anti-mouse Cy™3 (115-165-003, Jackson ImmunoResearch, 1:300), goat anti-rabbit Alexa Fluor Plus 647 (A32733 ...
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bioRxiv - Neuroscience 2024Quote: ... cyanine 3 (Cy3)-conjugated anti-goat (1:400; Jackson ImmunoResearch, West Grove, PA), cyanine 3 (Cy3)-conjugated anti-guinea pig (1:400 ...
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bioRxiv - Neuroscience 2024Quote: ... or donkey anti-rabbit Cyanine Dye 3 (Cy3) (1:300, Jackson ImmunoResearch, UK) in PBS/1% Triton X-100 in darkness for 3 h at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... Selected slices were then blocked for 3 h at room temperature in a PBS solution containing 5% normal donkey serum (NDS; Jackson ImmunoResearch) and 1% of non-ionic detergent (Triton® X-100 ...
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bioRxiv - Microbiology 2021Quote: ... cells were rinsed 3 times for 5 min in PBS and incubated for 30 min with a cyanine 3-conjugated goat anti-mouse secondary antibody (Jackson Immunoresearch) and DAPI (4′,6-diamidino-2-phenylindole).
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bioRxiv - Immunology 2023Quote: ... sections were incubated with 0.3% Triton-X in PBS (wash buffer) for 30 minutes for permeabilization and then with 5% Normal goat serum (Jackson ImmunoResearch) in wash buffer (blocking buffer) ...
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bioRxiv - Neuroscience 2024Quote: ... cells were carefully washed 3×5 min with PBS and incubated with secondary antibodies (Cat.# 711-545-152, 711-605-152, Jackson ImmunoResearch) for 1 h at RT ...
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bioRxiv - Neuroscience 2022Quote: ... and 36 µg rabbit anti-HA-tag (Cell Signalling Technology #3724) followed by fluorescently conjugated secondary antibodies in a 3:2 primary:secondary molar ratio (Jackson ImmunoResearch). Samples were incubated in EasyIndex (LifeCanvas Technologies ...
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bioRxiv - Cell Biology 2022Quote: ... in 1% (w/v) BSA 2% donkey serum (Jackson Immunoresearch, West Grove ...
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bioRxiv - Neuroscience 2020Quote: ... Primary antibody was applied and incubated overnight at 4°C followed by a biotinylated secondary goat anti-mouse antibody (Jackson Immunoresearch) incubation for 20 minutes at room temperature and incubation with a Streptavidin/HRP label (Jackson Immunoresearch) ...
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bioRxiv - Neuroscience 2021Quote: ... samples were incubated for 4 h at 25°C with fluorescent secondary antibody Rhodamine Red-X-AffiniPure Goat Anti-Rabbit IgG (Jackson ImmunoResearch Inc ...
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bioRxiv - Neuroscience 2022Quote: ... Gels were stored in PBS at 4 °C until subsequent treatments. Bovine serum albumin (BSA; catalog no. 001-000-162) was purchased from Jackson ImmunoResearch and NP-40 (catalog no ...
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bioRxiv - Molecular Biology 2020Quote: ... non-fat milk in PBS containing 0.1% Tween-20 for 1 hour and incubated with primary antibodies at 4 °C overnight followed by incubation with peroxidase-conjugated secondary antibodies (donkey anti-rabbit or donkey anti-mouse IgG, Jackson Immunoresearch). The proteins were detected using either Super Signal West Femto Maximum Sensitivity Substrate (Thermo Fisher ...
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bioRxiv - Neuroscience 2020Quote: ... in 500 μL of RIPA buffer were incubated overnight at 4°C with unspecific mouse/rabbit/rat IgG antibodies (Jackson Immunoresearch) or rat anti-HA-tag (Roche Diagnostics ...
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bioRxiv - Immunology 2024Quote: ... The plates were washed and incubated for 24 h at 4°C with HRP-conjugated anti-mouse IgG3 (Jackson ImmunoResearch Inc.) diluted 1/10000 in RPMI 10% FBS ...
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bioRxiv - Immunology 2023Quote: ... Antibody-coated cells were then cross-linked with 4 μg/ml AffiniPure goat-anti-mouse IgG+IgM (H+L; Jackson ImmunoResearch) and incubated for 15 minutes at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... washed and incubated for another 30 min at 4°C with APC-labelled donkey-anti-human-IgG (Jackson ImmunoResearch Europe Ltd.). For complement deposition assays ...
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bioRxiv - Plant Biology 2024Quote: ... and the roots were incubated overnight at 4°C with a mixture of secondary anti-rabbit conjugated with CY3 and anti-rat conjugated with CY2 (Jackson ImmunoResearch) each diluted to final concentration 1:500 ...
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bioRxiv - Neuroscience 2024Quote: ... for overnight at 4 °C and incubated with secondary antibodies like donkey anti-Rabbit IgG-HRP (Jackson ImmunoResearch, 711-035-152), donkey anti-mouse IgG-HRP (Jackson ImmunoResearch ...
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bioRxiv - Cell Biology 2024Quote: ... and stained with the following secondary antibodies at 10 μg/mL at room temperature for 4 hours: FITC-conjugated anti-mouse secondary antibody (Jackson ImmunoResearch Laboratories ...
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bioRxiv - Neuroscience 2020Quote: ... Cy™ 3-conjugated donkey anti-rabbit (1:500; 711-006-152, Jackson ImmunoResearch), Cy™ 5-conjugated donkey anti-mouse (1:500 ...
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bioRxiv - Developmental Biology 2023Quote: ... The following secondary antibodies were used: Cy3 anti-mouse IgG (subclasses 1+2a+2b+3) 1:250 (Jackson ImmunoResearch), Alexa Fluor 488 anti-rabbit IgG (H+L ...
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bioRxiv - Cell Biology 2020Quote: ... Secondary antibodies against whole Ig or Ig light-chain conjugated to horseradish peroxydase or Cyanine-2 and −3 were purchased from Jackson ImmunoResearch. Phalloidin-Fluoprobes647 (FP-BA0320 ...
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bioRxiv - Neuroscience 2024Quote: ... free-floating brain sections were blocked in 3% normal donkey serum for 2 h (Jackson ImmunoResearch Laboratories, Inc., West Grove, PA) and then incubated with rabbit anti-DsRed primary antibody (1:1,000 ...
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bioRxiv - Immunology 2020Quote: ... Membranes were incubated in primary antibodies for 1-3 hours at room temperature or overnight at 4°C followed by incubation in 20 ng/ml secondary horseradish-peroxidase (HRP)-conjugated secondary antibodies (Jackson ImmunoResearch Laboratories) for 45 min at room temperature ...
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bioRxiv - Physiology 2024Quote: ... treated with 1M HCl for 1 hour and incubated overnight at 4°C with anti-BrdU antibody following incubation with Alexa Fluor-488 conjugated goat anti-mouse IgG (Jackson ImmunoResearch Laboratories). Slides were mounted in mounting medium for fluorescence with DAPI (Vector Laboratories) ...
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bioRxiv - Bioengineering 2022Quote: ... or 5-tagged secondary antibodies raised in donkey [1:500 (Jackson ImmunoResearch), at 22–24°C for 2 h] ...
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bioRxiv - Immunology 2021Quote: ... cells were incubated for 5 mins with 2% (vol/vol) normal mouse and normal rat serum (Jackson ImmunoResearch Laboratories), prior to staining with a 2X master mix containing fluorophore-labeled antibodies at pre-titrated concentrations in Brilliant Violet Staining Buffer (BD Biosciences) ...
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bioRxiv - Neuroscience 2023Quote: ... anti-mouse unconjugated F(ab’)2 fragments (1:300, Jackson ImmunoResearch), were added in the blocking step ...
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bioRxiv - Neuroscience 2022Quote: ... donkey anti-mouse IgG (H+L) (Cy 3; 715-165-151, Jackson Immunoresearch; 1:800), and donkey anti-rabbit IgG (H+L ...
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bioRxiv - Neuroscience 2021Quote: ... sections were rinsed in PBS (5 x 5 min) and incubated in donkey anti-rabbit Cy5 secondary (1:1000 in PBS, Jackson ImmunoResearch, AB_2340607) for 1 hour ...
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bioRxiv - Cell Biology 2021Quote: ... Membranes were incubated with primary antibodies (4°C overinight) and then secondary peroxidase-conjugated Goat Anti-Rabbit IgG (Jackson ImmunoResearch 111-035-144) or peroxidase-conjugated Goat Anti-Mouse IgG (Jackson Immuno Research 115-035-174 ...
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bioRxiv - Cell Biology 2022Quote: ... 4 µg/ml) and donkey (711-166-152, 711-546-152, 715-166-150 or 715-546-151, Jackson ImmunoResearch, 1.5 µg/ml) secondary antibodies ...
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bioRxiv - Biochemistry 2024Quote: ... cells were washed once with FACS buffer and were labelled for 30 minutes at 4 °C with either Biotin-SP-conjugated AffiniPure Donkey Anti-Mouse IgG (H+L) (Jackson Immunoresearch Laboratories, Inc.) for samples 1 and 2 or with FACS buffer for sample 3 ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were blocked for 2 hours at room temperature (RT) in 5% normal goat serum (Jackson ImmunoResearch, West Grove, PA). Cells were incubated in one of the following primary antibodies in blocking solution containing 5% normal serum at 4°C overnight ...
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Sparcl1/Hevin drives inflammatory and neuropathic pain through astrocyte and NMDA receptor signalingbioRxiv - Neuroscience 2022Quote: ... cyanine 5(Cy5)- and/or FITC-conjugated secondary antibodies (1:400; Jackson ImmunoResearch) for 2 h at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... followed for 1 hour blocking in 5% Normal Donkey Serum (NDS, Jackson ImmunoResearch) and 0.05% Triton X-100 in PBS ...
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bioRxiv - Cell Biology 2023Quote: ... 1: 10 000 in 5% milk/TBSP (Jackson ImmunoResearch, West Grove, PA, USA). HA-tagged proteins were detected using a Westar Supernova kit (Cyanagen ...
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bioRxiv - Developmental Biology 2024Quote: ... Cells were then incubated at 4°C overnight with primary antibody at 1:100 in blocking buffer The following day cells were washed 3 times for 5 minutes with 1X TBS then incubated with Cy5 Donkey Anti-Mouse (Jackson ImmunoResearch 715-175-150) at 1:100 in 1X TBS for 2 hours in the dark at room temperature ...
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bioRxiv - Immunology 2023Quote: ... Endogenous peroxidase activity was blocked with 3% hydrogen peroxide and sections were blocked with 2% BSA (Jackson ImmunoResearch Lab, Cat# 001-000-162) for 1h at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... and Cy 3-Conjugated AffiniPure Goat Anti-Mouse IgG (Jackson ImmunoResearch, #115-165-146, 1:1000).
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bioRxiv - Bioengineering 2022Quote: ... and Caspase-3 cells were visualized using Alexa Fluor 488 secondary antibody (Jackson ImmunoResearch, 1:400). Cell counts were computed in the motor cortex using QuPath (University of Edinburgh) ...
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bioRxiv - Systems Biology 2021Quote: ... and blocked with 1% BSA in carbonate buffer before being incubated overnight at 4°C with rabbit anti-sheep IgGs in carbonate buffer (Jackson ImmunoResearch, #313-005-003). 15 µL of cell lysate was incubated for 3 h at 4°C in the IgG-coated 384-well plates ...
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bioRxiv - Biochemistry 2021Quote: ... cells were treated with 1 μM biotinylated peptides for 1h before they were fixed with 4% PFA and incubated with Streptavidin-AlexaFluor 594 antibody (Jackson ImmunoResearch, West Grove, PA) and Hoechst 33342 (Novus Biologicals ...
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bioRxiv - Cell Biology 2023Quote: ... overnight at 4°C and then with 0.1 μg/ml Alexa Fluor 594-conjugated donkey polyclonal anti-rabbit IgG antibody (Jackson ImmunoResearch, West Grove, PA, USA) for 1 h at room temperature [6-8,10] ...
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bioRxiv - Systems Biology 2023Quote: ... and blocked with 1% BSA in carbonate buffer before being incubated overnight at 4°C with rabbit anti-sheep IgGs in carbonate buffer (Jackson ImmunoResearch, #313-005-003). 15 µL of cell lysate was incubated for 3 h at 4°C in the IgG-coated 384-well plates ...
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bioRxiv - Neuroscience 2023Quote: ... Slides were then washed 4 × 30s in a tris buffer solution and the secondary biotinylated mouse adsorbed donkey anti-rabbit antibody (Jackson ImmunoResearch, 711-065-152) applied to all sections and incubated for 30 min ...