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Citations for Jackson ImmunoResearch :
151 - 200 of 394 citations for 4 4 Methylphenyl 1H pyrrole 3 carbonitrile since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... cells were incubated for 1h at RT with blocking solution of 5% goat serum in PBS (Jackson ImmunoResearch, catalog no. 005–000-121). A rabbit polyclonal anti-SARS-CoV-2 nucleocapsid antibody (GeneTex ...
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bioRxiv - Immunology 2024Quote: ... washed and overlaid with HRP-conjugated goat anti-mouse secondary antibodies diluted 1:5,000 in blocking solution for 1h at RT (Jackson ImmunoResearch, #115-035-003) and subsequently washed ...
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bioRxiv - Cancer Biology 2022Quote: ... Membranes were washed three times with 0.1% PBS-T and were incubated with secondary horseradish peroxidase-coupled antibodies for 1h at RT (GE Healthcare, Jackson ImmunoResearch, 1:10000). ECL Western Blotting Detection Reagent (RPN2106 ...
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bioRxiv - Developmental Biology 2020Quote: ... the embryos were incubated overnight at 4 °C in Cy3-conjugated goat anti-rabbit antibody (1:500 in BB; Jackson Immunoresearch, 111-165-144), Alexa488-conjugated goat anti-mouse (1:500 in BB ...
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bioRxiv - Systems Biology 2021Quote: ... and blocked with 1% BSA in carbonate buffer before being incubated overnight at 4°C with rabbit anti-sheep IgGs in carbonate buffer (Jackson ImmunoResearch, #313-005-003). 15 µL of cell lysate was incubated for 3 h at 4°C in the IgG-coated 384-well plates ...
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bioRxiv - Neuroscience 2020Quote: ... and the following secondary antibodies at 4°C for 24 h: Alexa Fluor 488 donkey anti-chicken (1:250, Jackson ImmunoResearch, #703-545-155) and Alexa Fluor 568 donkey anti-rabbit (1:250 ...
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bioRxiv - Neuroscience 2020Quote: ... the sections were incubated for 4-6 h in the following secondary antibodies: donkey anti-rat 488 (1: 500; Jackson Immunoresearch, 712-546-153), donkey anti-rabbit-Cy3 (1:500 ...
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bioRxiv - Cell Biology 2023Quote: ... overnight at 4°C and then with 0.1 μg/ml Alexa Fluor 594-conjugated donkey polyclonal anti-rabbit IgG antibody (Jackson ImmunoResearch, West Grove, PA, USA) for 1 h at room temperature [6-8,10] ...
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bioRxiv - Systems Biology 2023Quote: ... and blocked with 1% BSA in carbonate buffer before being incubated overnight at 4°C with rabbit anti-sheep IgGs in carbonate buffer (Jackson ImmunoResearch, #313-005-003). 15 µL of cell lysate was incubated for 3 h at 4°C in the IgG-coated 384-well plates ...
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bioRxiv - Genomics 2023Quote: ... for 1 hour, followed by incubation with primary antibodies (overnight, 4°C) and secondary antibodies (1-2 hours, from Jackson ImmunoResearch or ThermoFisher Scientific). The slides were then mounted with coverslips using VECTASHIELD (Vector Labs ...
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bioRxiv - Bioengineering 2022Quote: ... Staining with primary antibodies was performed for 24 h (at 4 °C) with subsequently 2 h incubation with secondary antibodies (Jackson ImmunoResearch, West Grove, PA) diluted with 1 % BSA ...
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated overnight at 4°C in primary antibody solution containing 2% Normal Donkey Serum (NDS; Jackson Immunoresearch Laboratories Inc. 017-000-121) + 1xPBS + .03% Triton + primary antibodies (chicken anti-Iba1 ...
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bioRxiv - Neuroscience 2023Quote: ... Slides were then washed 4 × 30s in a tris buffer solution and the secondary biotinylated mouse adsorbed donkey anti-rabbit antibody (Jackson ImmunoResearch, 711-065-152) applied to all sections and incubated for 30 min ...
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bioRxiv - Neuroscience 2023Quote: ... slides were washed 3X 15 min in 0.1M PBS and then incubated at 4°C for 24 h with donkey anti-chicken-488 (1:1000; 703-545-155, Jackson Immunoresearch, West Grove, PA) and donkey anti-rabbit-CY3 (1:400 ...
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bioRxiv - Developmental Biology 2022Quote: ... Larval brains were washed twice with 0.5% PBS-T and incubated for 1-7 days at 4°C in a blocking solution of 1% goat serum (Jackson ImmunoResearch, West Grove, PA), 1% donkey serum (Jackson ImmunoResearch ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated overnight at 4°C in primary antibody solution containing 2% Normal Donkey Serum (NDS; Jackson Immunoresearch Laboratories Inc. 017-000-121) + 1xPBS + 0.03% Triton + primary antibody (rabbit anti-P2RY12 ...
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bioRxiv - Immunology 2021Quote: ... cells were blocked with 0.22 µm-filtered 5% Donkey Serum for 1 h at RT before staining for 1h with AF647 AffiniPure F(ab’)□ Fragment Donkey Anti-Rabbit IgG (H+L) (Jackson ImmunoResearch, #711-606-152). After four washes ...
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bioRxiv - Genomics 2020Quote: ... Three washes of 5 minutes each on rotator were done using TBSt before and after the 1h incubation of the membranes with 1:1000 goat anti-mouse-HRP (Jackson Immunoresearch, 115-035-003) in 2%SM in TBSt ...
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bioRxiv - Immunology 2021Quote: ... for 1 h at room temperature followed by incubation for 1h at room temperature with anti-human peroxidase conjugate IgG (Jackson ImmunoResearch, Ely, Cambridgeshire, UK) or with anti-rabbit horseradish peroxidase (HRP)-conjugated IgG (Bio-Rad ...
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bioRxiv - Immunology 2024Quote: ... The following day slides were washed with PBS-T and incubated for 1h at rt with 15 μg/ml fab fragment anti-rabbit alexa 647 (Jackson ImmunoResearch, 711-607-003). Slides were stained overnight with 33 μg/ml anti-IgA alexa488 (Abcam ...
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bioRxiv - Immunology 2021Quote: ... for 30 minutes at 4°C and subsequently labeled with Rhodamine conjugated anti-mouse IgG (Jackson ImmunoResearch, Cat#115-026-062, 1:100 in PBS). Fluorescently labeled nuclei were counted using a Nikon Eclipse TE2000-U at 20x magnification.
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bioRxiv - Neuroscience 2021Quote: ... above tissue sections under ISH were incubated with chicken anti-NeuN antibody (1:200; Aves, Davis, CA) overnight at 4°C followed by donkey anti-chicken IgG Alexa 488 (Jackson ImmunoResearch labs, West Grove, PA) for 1 hr at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were rinsed in PBS and incubated at 4°C in PBS containing 2% donkey serum and secondary antibodies conjugated to anti-rabbit Cy3 (Jackson ImmunoResearch Inc., West Grove, PA) and anti-guinea pig CF405M (Biotium ...
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bioRxiv - Cell Biology 2020Quote: ... Blotted proteins were incubated with corresponding primary antibodies at 4°C overnight followed by incubation with horseradish peroxidase conjugated secondary antibodies (Jackson ImmunoResearch, West Grove, PA, USA). Amersham ECL Western blotting detection reagent (GE Healthcare Life Sciences ...
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bioRxiv - Cancer Biology 2023Quote: ... for 10 minutes and washed 3 times in PBS for 5 minutes at room temperature (RT), followed by incubation for 30 minutes in blocking buffer (1% IgG-free BSA, 4% donkey serum (Jackson ImmunoResearch, West Grove, PA, USA) in PBS) ...
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bioRxiv - Neuroscience 2022Quote: ... Cat#ACC-102) over night at 4°C followed by 1 hour probing with donkey anti-rabbit HRP (1:10,000; Jackson ImmunoResearch Labs Cat#711-035-152). The membrane were imaged via enhanced chemiluminescence photodetection with the BioRad ChemiDoc XRS+ system.
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bioRxiv - Neuroscience 2024Quote: ... Membranes were incubated overnight at 4°C with the primary antibodies and then with the corresponding horseradish peroxidase-conjugated secondary antibodies (Jackson Immunoresearch, West Grove, PA, USA). All membranes were revealed using ECL chemiluminescence kit according to the manufacturer’s instructions (GE Healthcare ...
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bioRxiv - Neuroscience 2024Quote: ... and donkey anti-guinea pig Alexa Fluor (AF) 488 (Catalog #:706-545-148) had a 1:500 dilution and were stored overnight at 4 °C (Jackson ImmunoResearch; West Grove, PA, USA). Sections were then mounted and cover-slipped with using 50% glycerol in 0.02M KPBS and clear nail polish was used to seal the coverslip onto the slide.
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bioRxiv - Developmental Biology 2023Quote: ... + 3% donkey serum (Jackson ImmunoResearch)] at room temperature for 15 mins ...
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bioRxiv - Neuroscience 2021Quote: ... Primary antibody incubation was followed by washes and incubation with secondary antibody for 1h (HRP-conjugated goat anti-rabbit IgG, and HRP-conjugated goat anti-mouse; Jackson ImmunoResearch Laboratories, West Grove, PA). Protein bands were visualized using the ECL detection system (Thermo Fisher Scientific ...
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bioRxiv - Physiology 2023Quote: ... Cells were then washed and incubated with primary antibodies diluted in blocking solution overnight at 4 °C followed by incubation with an AlexaFluor 488-conjugated AffiniPure Donkey Anti-Rabbit IgG secondary antibody (Jackson ImmunoResearch, 711-545-152, 1:500) for 1h at RT ...
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bioRxiv - Neuroscience 2022Quote: ... Pre-prepared immunopanning dishes were setup as follows: a 10cm petri dish was incubated at 4°C overnight with 30ul anti-rat antibody (Jackson ImmunoResearch Laboratories, Inc, 112-005-167) in 10 ml 50 mM Tris-HCL (pH 9.5) ...
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bioRxiv - Cell Biology 2023Quote: ... cells were fixed with 4% paraformaldehyde and immunofluorescence was performed as described previously [54] using appropriate primary and secondary antibodies (Jackson ImmunoResearch Laboratories, West Grove, PA, USA), and coverslips were mounted using Prolong Gold Antifade Reagent with 4′,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Developmental Biology 2021Quote: ... The membranes were blocked with 3% nonfat milk and 3% bovine serum albumin (Jackson ImmunoResearch) in Tris-Buffered Saline Tween-20 and probed with antibodies against the cytoplasmic domain of NOTCH4 [23] and β-ACTIN (Abclonal) ...
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bioRxiv - Molecular Biology 2021Quote: ... washed again and blocked in 3% fetal bovine serum/3% normal goat serum (Jackson ImmunoResearch). Primary antibody (CD44 ...
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bioRxiv - Developmental Biology 2021Quote: ... Embryos were washed in PBST four times for 4 hours and incubated overnight with biotin goat anti-mouse IgG (Jackson ImmunoResearch ref. 115-066-071; 1:500) and then with streptavidin-Cy3 (Jackson ImmunoResearch ref ...
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bioRxiv - Bioengineering 2024Quote: ... Samples were washed five times with PTW and subsequently incubated with the respective secondary antibodies (1:500) for 24 h at 4°C (donkey anti-chicken Alexa Fluor 488 (Jackson ImmunoResearch Europe Ltd., Cat#: 703-545-155)) ...
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bioRxiv - Cell Biology 2023Quote: ... samples were then transferred directly into blocking buffer (4% BSA (Sigma Cat# 10735086001)/3% goat serum (Jackson Immunoresearch #005000121)/PBS) ...
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bioRxiv - Developmental Biology 2023Quote: ... slides were washed 3×3 minutes in PBS then stained with secondary antibody (Jackson ImmunoResearch, 1:500) diluted in the blocking buffer for 1 hour at room temperature ...
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bioRxiv - Developmental Biology 2023Quote: ... slides were washed 3×3 minutes in PBS then stained with secondary antibody (Jackson ImmunoResearch, 1:500) diluted in PBS for 1 hour at room temperature ...
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bioRxiv - Immunology 2023Quote: ... the cells were stained for 30 minutes at 4°C with a fluorescently labeled secondary antibody – anti-human-IgG-F(ab’)2 (Jackson ImmunoResearch #109-606-097, used at 1:400) for Nivolumab and Durvalumab-stained samples ...
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bioRxiv - Neuroscience 2020Quote: ... and 3% Normal Donkey Serum (Jackson Immunoresearch). Retinas were then stained with primary antibody for 5-7 days at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... and 3% Normal Donkey Serum (Jackson Immunoresearch). Sections were incubated in primary antibodies overnight ...
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bioRxiv - Developmental Biology 2022Quote: ... Cy-3 or Cy-5 (Jackson Immunoresearch Laboratories), and processed for DAPI or Hoechst staining to visualize cells’ nuclei before mounting in ImmuMount medium (Shandon) ...
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bioRxiv - Cell Biology 2022Quote: ... mEos2::HIM-3 was co-stained with a rabbit anti-HIM-3 antibody and a donkey-anti-rabbit secondary antibody (Jackson ImmunoResearch) labelled with CF568-NHS ester to achieve a 2:1 dye-to-antibody ratio ...
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bioRxiv - Molecular Biology 2020Quote: ... Slides were washed 3 times for 3 minutes in PBST each and incubated in biotinylated rabbit antibody (Jackson Immunoresearch #711-065-152) 1:250 dilution in blocking solution for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... blocked for 2-3 hours (0.3% Triton X-100, American Bioanalytical, Canton, MA; 3% normal donkey serum, Jackson ImmunoResearch, West Grove, PA), then incubated overnight with primary antibodies (1:1000 + 1% normal donkey serum) ...
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bioRxiv - Molecular Biology 2021Quote: ... anti-mouse Cyanine 3 (115-167-003, Jackson ImmunoResearch), anti-rabbit Cyanine 3 (111-166-045 ...
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bioRxiv - Neuroscience 2020Quote: ... and 3% Donkey Serum (Jackson ImmunoResearch, 017-000-121) at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... Cy-3 or Cy-5 (1:500, Jackson ImmunoResearch). Sections were counterstained with a fluorescent Nissl stain (NeuroTrace 435/445 blue ...