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Citations for Jackson ImmunoResearch :
101 - 150 of 394 citations for 4 4 Methylphenyl 1H pyrrole 3 carbonitrile since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... Gels were stored in PBS at 4 °C until subsequent treatments. Bovine serum albumin (BSA; catalog no. 001-000-162) was purchased from Jackson ImmunoResearch and NP-40 (catalog no ...
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bioRxiv - Molecular Biology 2020Quote: ... non-fat milk in PBS containing 0.1% Tween-20 for 1 hour and incubated with primary antibodies at 4 °C overnight followed by incubation with peroxidase-conjugated secondary antibodies (donkey anti-rabbit or donkey anti-mouse IgG, Jackson Immunoresearch). The proteins were detected using either Super Signal West Femto Maximum Sensitivity Substrate (Thermo Fisher ...
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bioRxiv - Neuroscience 2020Quote: ... in 500 μL of RIPA buffer were incubated overnight at 4°C with unspecific mouse/rabbit/rat IgG antibodies (Jackson Immunoresearch) or rat anti-HA-tag (Roche Diagnostics ...
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bioRxiv - Neuroscience 2020Quote: ... followed by 2 days at 4°C (iii) 1:400 of biotinylated anti-goat IgG (Jackson ImmunoResearch, catalog 705-065-147), 30 minutes ...
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bioRxiv - Neuroscience 2021Quote: ... sections were incubated for 4 h with secondary antibodies (anti-rat Cy3, anti-mouse DyLight 488, anti-rabbit Cy5, 1:500, Jackson Immunoresearch) diluted in the blocking solution ...
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bioRxiv - Neuroscience 2021Quote: ... Primary antibody was washed off 3 times in PBT and incubated in secondary antibody shaking overnight at 4 °C (1:500 488-555-680-Donkey Anti-Rabbit (Jackson ImmunoResearch) in PBT + 1% BSA) ...
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bioRxiv - Neuroscience 2021Quote: ... Primary antibody was washed off 3 times in PBT and incubated in secondary antibody shaking overnight at 4°C (1:500 488-555-680-Donkey Anti-Rabbit (Jackson ImmunoResearch) in PBT + BSA) ...
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bioRxiv - Immunology 2021Quote: ... RBD antigen binding during selection Round 4 was evaluated using a 1:1000 dilution of Streptavidin-Alexa Fluor 647 secondary (Jackson ImmunoResearch). Use of a different secondary reagent selected for true RBD binders and de-enriched the population for anti-His-tag secondary reagent binders (data not shown) ...
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bioRxiv - Developmental Biology 2021Quote: ... and St18 (1:1000) were incubated in 5% NDS/PBS overnight at 4°C and secondary antibodies (Jackson Immunoresearch, 1:1000) diluted in 0.01% Triton X-100/PBS for 2 hours at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... washed and incubated for another 30 min at 4°C with APC-labelled donkey-anti-human-IgG (Jackson ImmunoResearch Europe Ltd.). For complement deposition assays ...
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bioRxiv - Cell Biology 2022Quote: ... overnight at 4°C, and with fluorescence (Alexa Fluor 488, 568, 647)-conjugated secondaries (Jackson ImmunoResearch Laboratories or Abcam; 1:1000) overnight at 4°C at indicated dilutions ...
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bioRxiv - Neuroscience 2022Quote: ... Slides were incubated in primary antibodies overnight at 4°C (Supplementary Table 2) and in biotinylated secondary antibodies (Jackson Immunoresearch Laboratories) for 2.5 hours at room temperature ...
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bioRxiv - Bioengineering 2022Quote: ... Fixed samples were permeabilized and blocked overnight at 4 °C with a blocking buffer containing 5% IgG-free bovine serum albumin (BSA) (Jackson ImmunoResearch) and 0.3% Triton X-100 (Bio Basic ...
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bioRxiv - Immunology 2024Quote: ... The plates were washed and incubated for 24 h at 4°C with HRP-conjugated anti-mouse IgG3 (Jackson ImmunoResearch Inc.) diluted 1/10000 in RPMI 10% FBS ...
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bioRxiv - Neuroscience 2023Quote: ... Immunohistochemistry was performed by incubating with primary antibodies (overnight, 4°C) and fluorophore-conjugated secondary antibodies (2 hr, room temperature; ThermoFisher, Jackson ImmunoResearch). Sections were mounted with VectaShield (VECTOR ...
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bioRxiv - Cell Biology 2023Quote: ... 1:5) at 4 °C overnight and with the secondary FITC-conjugated or TRITC-conjugated anti-mouse secondary antibody (Jackson ImmunoResearch Laboratories ...
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bioRxiv - Neuroscience 2023Quote: ... larvae were incubated in the dark at 4°C for 24 h with secondary antibodies (1:500, Alexa Fluors 488, 555, 594 and 647, Jackson Immunoresearch). For CASK immunostaining experiments larvae were incubated in Hoechst stain (1:8000 ...
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bioRxiv - Neuroscience 2023Quote: ... All sections were rinsed three times for 15 min in PBS-T and incubated for 4 hours at ∼20 °C in secondary antibodies in the blocking solution (Cy3 donkey anti-guinea pig, 1:400, Jackson ImmunoResearch, catalog no ...
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bioRxiv - Neuroscience 2023Quote: ... followed by incubation with the primary antibodies overnight at 4°C and then the proper secondary antibodies (1:500, Alexa Fluor 488, 594, 647 conjugated; Jackson ImmunoResearch) for 2 h at room temperature in the same blocking solution ...
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bioRxiv - Immunology 2023Quote: ... Antibody-coated cells were then cross-linked with 4 μg/ml AffiniPure goat-anti-mouse IgG+IgM (H+L; Jackson ImmunoResearch) and incubated for 15 minutes at 37°C ...
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bioRxiv - Neuroscience 2024Quote: ... for overnight at 4 °C and incubated with secondary antibodies like donkey anti-Rabbit IgG-HRP (Jackson ImmunoResearch, 711-035-152), donkey anti-mouse IgG-HRP (Jackson ImmunoResearch ...
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bioRxiv - Plant Biology 2024Quote: ... and the roots were incubated overnight at 4°C with a mixture of secondary anti-rabbit conjugated with CY3 and anti-rat conjugated with CY2 (Jackson ImmunoResearch) each diluted to final concentration 1:500 ...
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bioRxiv - Neuroscience 2024Quote: Cells were fixed with 4% paraformaldehyde (PFA) and blocked for 1 hour in phosphate-buffered saline (PBS) supplemented with 10% normal donkey serum (Jackson ImmunoResearch) and 0.1% Triton X-100 ...
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bioRxiv - Genomics 2024Quote: ... and then incubated with primary antibodies in A+t buffer at 4°C overnight and then with fluorescently-labeled secondary antibodies (1:200 dilution, Jackson ImmunoResearch). Testes were stained with 0.5 μg/mL DAPI/PBS and mounted in 80% glycerol on slides ...
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bioRxiv - Cell Biology 2024Quote: ... and stained with the following secondary antibodies at 10 μg/mL at room temperature for 4 hours: FITC-conjugated anti-mouse secondary antibody (Jackson ImmunoResearch Laboratories ...
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bioRxiv - Neuroscience 2020Quote: ... goat anti-mouse IgG1 Alexa-488 and donkey anti-chicken Alexa-488 were incubated together for 1h at RT (all 1:500; Jackson Immunoresearch). All antibody dilutions were made in PBS buffer containing 0.01% Triton X-100 and 2% NDS ...
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bioRxiv - Pathology 2022Quote: ... Membranes were washed (1x in TBS-T) and incubated (1h, RT) with peroxidase-conjugated secondary antibodies: AffiniPure Goat Anti-Rabbit IgG (H+L; polyclonal)(Jackson Immunoresearch, #111-035-045 ...
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bioRxiv - Immunology 2023Quote: ... incubated for 1h at RT with HRP-conjugated goat anti-mouse secondary antibodies (115-035-003, dilution 1:5000, Jackson ImmunoResearch) or anti-rabbit (111-035-003 ...
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bioRxiv - Immunology 2023Quote: ... incubated for 1h at RT with HRP-conjugated goat anti-mouse secondary antibodies (115-035-003, dilution 1:5000, Jackson ImmunoResearch) or anti-rabbit (111-035-003 ...
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bioRxiv - Neuroscience 2020Quote: ... Slides were incubated in primary antibodies overnight at 4°C (Extended Data Table 1) and in biotinylated secondary antibodies (Jackson Immunoresearch Laboratories) for 2.5 hours at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... cells were washed with PBS and blocked with 2% BSA (w/v) (Roth) and 4% donkey serum (v/v) (Jackson Immunoresearch Laboratories) in PBS for 30 min at RT ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were rinsed three times in PBS and incubated for 60–90 min at room temperature or overnight at 4°C with the Alexa Fluor 488- and 594 and 647-conjugated secondary antibodies (1:500; Thermo Fisher Science or Jackson ImmunoResearch).
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bioRxiv - Immunology 2020Quote: ... Membranes were incubated in primary antibodies for 1-3 hours at room temperature or overnight at 4°C followed by incubation in 20 ng/ml secondary horseradish-peroxidase (HRP)-conjugated secondary antibodies (Jackson ImmunoResearch Laboratories) for 45 min at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... primary antibody overnight at 4°C and secondary antibody (anti-rabbit-Alexa Fluor 647, 1:400, Jackson ImmunoResearch, Inc., West Grove, PA) for 3-4 hours in the dark ...
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bioRxiv - Neuroscience 2023Quote: ... the cells were incubated with 4’,6-diamidino-2-phenylindol (DAPI; 1 μg/ml) and the corresponding Alexa Fluor-conjugated secondary antibodies (1:500; Jackson ImmunoResearch Laboratories) for 2 h at RT ...
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bioRxiv - Physiology 2023Quote: ... slices were washed 3×5 min in PBS 0.01M at RT – 4 mL/well and incubated in donkey anti-guinea pig-AF647 (Jackson ImmunoResearch, 1:500) diluted in [PBS 0.01M + 0.3% Triton] ...
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bioRxiv - Neuroscience 2023Quote: ... followed by a 4 h incubation with a secondary donkey anti-guinea pig IgG [F(ab’)2fragments] (1:500, Jackson ImmunoResearch #706606148). Tissues were rinsed and mounted using Prolong diamond antifade (Life Technologies) ...
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bioRxiv - Physiology 2024Quote: ... treated with 1M HCl for 1 hour and incubated overnight at 4°C with anti-BrdU antibody following incubation with Alexa Fluor-488 conjugated goat anti-mouse IgG (Jackson ImmunoResearch Laboratories). Slides were mounted in mounting medium for fluorescence with DAPI (Vector Laboratories) ...
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bioRxiv - Biophysics 2024Quote: ... Nonspecific cross-reactivity was blocked by incubating the samples for 4 h in 1% BSA/PBS (Jackson ImmunoResearch, West Grove, PA, USA). The cells were then incubated with primary antibodies anti-RyR (IgG1 ...
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bioRxiv - Neuroscience 2021Quote: ... animals were incubated overnight at 4°C in Alexa Fluor 488 donkey anti-chicken secondary antibody (1:1000, Jackson ImmunoResearch, 703-545-155), Alexa Fluor 594 goat anti-mouse secondary antibody (1:1000 ...
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bioRxiv - Microbiology 2020Quote: ... Cells are incubated with mAb 4G2 (1 µg/mL) for 1hour at 4 °C followed by secondary anti-mouse Alexa 488 (Jackson Immunoresearch, 1:50000). After washing with PBS ...
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bioRxiv - Neuroscience 2021Quote: ... overnight at 4°C followed by the addition of a goat anti-rat HRP-conjugated secondary antibody (Jackson ImmunoResearch, Westgrove, PA; 1:10,000) for 1 hr ...
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bioRxiv - Neuroscience 2022Quote: ... 0.2% Triton X-100 and incubated overnight at 4°C with a CY5-conjugated secondary antibody (1:400, goat anti-mouse, Jackson Immunoresearch, Suffolk, UK), washed in 1x PBS and embedded in 1x PBS ...
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bioRxiv - Microbiology 2021Quote: ... Labeled cells were washed with ice-cold PBS-BSA followed by secondary labeling for 1 h at 4°C in 2.5 mL 1:200 PE-conjugated goat anti-human-IgG (Jackson ImmunoResearch 109-115-098) to label for bound monoclonal antibody or 1:200 Alexa-647-conjugated goat anti-human-IgA+IgG+IgM (Jackson ImmunoResearch 109-605-064 ...
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bioRxiv - Microbiology 2021Quote: ... samples were further statically incubated for 1 h at 4°C with APC-conjugated polyclonal goat-anti-human IgG F(ab’)2 antibody (Jackson immunoresearch, 1:500). Fab fragments were detected with Alexa Fluor 647 conjugated goat-anti-human-kappa F(ab’)2 antibody (Southern Biotech ...
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bioRxiv - Microbiology 2020Quote: ... Labeled cells were washed with ice-cold PBS-BSA followed by secondary labeling for 1 h at 4°C in 2.5 mL 1:200 PE-conjugated goat anti-human-IgG (Jackson ImmunoResearch 109-115-098) to label for bound antibody ...
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bioRxiv - Cell Biology 2021Quote: ... Membranes were incubated with primary antibodies (4°C overinight) and then secondary peroxidase-conjugated Goat Anti-Rabbit IgG (Jackson ImmunoResearch 111-035-144) or peroxidase-conjugated Goat Anti-Mouse IgG (Jackson Immuno Research 115-035-174 ...
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bioRxiv - Cell Biology 2022Quote: ... 4 µg/ml) and donkey (711-166-152, 711-546-152, 715-166-150 or 715-546-151, Jackson ImmunoResearch, 1.5 µg/ml) secondary antibodies ...
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bioRxiv - Cell Biology 2023Quote: ... at 4°C for 1 h following incubation with fluorescein (FITC) AffiniPure goat anti-rat IgM (1:100, Jackson ImmunoResearch, 112-095-075) or allophycocyanin-conjugated (APC ...
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bioRxiv - Biochemistry 2024Quote: ... cells were washed once with FACS buffer and were labelled for 30 minutes at 4 °C with either Biotin-SP-conjugated AffiniPure Donkey Anti-Mouse IgG (H+L) (Jackson Immunoresearch Laboratories, Inc.) for samples 1 and 2 or with FACS buffer for sample 3 ...