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Citations for Jackson ImmunoResearch :
701 - 750 of 857 citations for 5 Bromo 2 bromo difluoro methyl 1H benzimidazole since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... or cultured cells stained for the expression of surface markers with specific antibodies in PBS with 5% FBS and 20% normal goat serum (Jackson ImmunoResearch 005-000-121) and 0.01% NaN3 for 20 min on ice ...
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bioRxiv - Developmental Biology 2023Quote: ... Secondary antibody incubation was performed in 5% milk for 1 hour at room temperature using 1:5000 for anti-rabbit-HRP (Jackson Immunoresearch, 711-035-152) or anti-mouse-HRP (Jackson Immunoresearch ...
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bioRxiv - Neuroscience 2023Quote: ... the tissue was washed six times in PBS (5 min each) then incubated with a biotinylated goat anti-rabbit antibody (1:500; Jackson ImmunoResearch Laboratories; RRID: AB_2337965) prepared in NDS for 1 hr (RT) ...
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bioRxiv - Microbiology 2022Quote: ... The grids were washed on three drops of PBS before being incubated for 12 min with a 5 nm gold-labelled anti-rabbit secondary antibody (Jackson ImmunoResearch West Grove, PA) at dilutions of 1:10 ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were blocked for 1 hour with block buffer (0.05% IGEPAL CA-630, 0.05% Triton X-100, 5% goat normal serum [Jackson ImmunoResearch; 005-000-121] in 1xPBS). Samples were incubated with primaries/nanobodies diluted in block buffer for 16 hours at 4 °C on a rocker ...
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bioRxiv - Developmental Biology 2023Quote: ... The cells were then washed with PBS three times (5 minutes each) and incubated with Alexa Fluor 488 anti-mouse secondary antibody (1:1000, Jackson Immunoresearch #115-545-146) at 4°C overnight ...
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bioRxiv - Developmental Biology 2024Quote: ... Cells were then incubated at 4°C overnight with primary antibody at 1:100 in blocking buffer The following day cells were washed 3 times for 5 minutes with 1X TBS then incubated with Cy5 Donkey Anti-Mouse (Jackson ImmunoResearch 715-175-150) at 1:100 in 1X TBS for 2 hours in the dark at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... sections were washed in PBS (3×5 min) and incubated overnight with secondary antibody (peroxidase conjugated donkey-anti-rabbit 1:500, Jackson Immunoresearch, Baltimore, PA, USA). The Tyramide Signal Amplification procedure was then employed ...
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bioRxiv - Bioengineering 2024Quote: ... The cells were washed with 1X DPBS and then incubated in blocking buffer (consisting of 5% (v/v) normal donkey serum (Jackson ImmunoResearch, West Grove, PA) and 1% (w/v ...
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bioRxiv - Cell Biology 2024Quote: ... The following secondary antibodies were used at 1:10,000 dilution and incubated for 1 hour at room temperature in 5% milk w/ TBST: HRP donkey anti-rabbit (Jackson ImmunoResearch #711-035-152) and HRP goat anti-mouse IgG subclass 1-specific (Jackson ImmunoResearch #115-035-205) ...
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bioRxiv - Cancer Biology 2021Quote: ... for 30 min at room temperature and then with secondary antibodies against rabbit IgG (H+L) F(ab’)2 fragment conjugated to DyLight™ 649 (Jackson ImmunoResearch). p-ERK1/2 mean fluorescence intensities (MFIs ...
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bioRxiv - Cancer Biology 2021Quote: ... for 60 min and then secondary antibodies against rabbit IgG (H+L) F(ab’)2 fragment conjugated to DyLight™ 649 (Jackson ImmunoResearch) or mouse IgG (H+L ...
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bioRxiv - Cancer Biology 2021Quote: ... which was detected by the secondary antibody against rabbit IgG (H+L) F(ab’)2 fragment conjugated to DyLight™ 649 (Jackson ImmunoResearch) (30 min ...
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bioRxiv - Immunology 2022Quote: ... Beads were washed for the last time with PBS and resuspended in a solution of 150nM Goat anti-mouse IgM (μ chain specific) F(ab’)2 (Alexa647, Jackson ImmunoResearch) in DropMap buffer ...
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bioRxiv - Immunology 2020Quote: ... CAR expression was determined using a flow cytometry antibody specific for the murine F(ab’)2 fragment (Jackson ImmunoResearch, 115-606-006).
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bioRxiv - Neuroscience 2021Quote: ... After the brain slices were washed 3x with 1XPBS they were incubated with the following secondary antibodies in blocking buffer for 2 hours at room temperature: 1:500 dilution of Donkey anti-rat Alexa Fluor 488 (Jackson ImmunoResearch Laboratories), 1:200 dilution of Donkey anti-guinea pig Alexa Fluor 647 (VWR) ...
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bioRxiv - Neuroscience 2021Quote: ... brain sections were washed with KPBS and then incubated for 2 hours at room temperature in the dark with either a secondary antibody donkey anti-goat Alexa Fluor 647® (Jackson Immunoresearch) or donkey anti-rabbit Alexa Fluor 488® (Jackson Immunoresearch ...
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bioRxiv - Cell Biology 2021Quote: ... and incubated overnight with equal molar amounts of F(ab’)2 goat-anti-mouse IgM+G (αM, 20 μg/ml, Jackson ImmunoResearch Laboratories), hen egg lysozyme (HEL ...
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bioRxiv - Neuroscience 2020Quote: ... sections were incubated for 2 hour at RT with the biotinilated secondary antibody: donkey anti-mouse IgG (Jackson ImmunoResearch Laboratories, 1:200), followed by an avidin-biotin-peroxidase complex (ABC ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were then stimulated with 10 μg/ml F(ab’)2 Fragment Goat Anti-Human IgA + IgG + IgM (H+L) (Jackson ImmunoResearch, USA) for the indicated time points ...
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bioRxiv - Cell Biology 2021Quote: ... cells were washed with PBS and blocked with 2% BSA (w/v) (Roth) and 4% donkey serum (v/v) (Jackson Immunoresearch Laboratories) in PBS for 30 min at RT ...
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bioRxiv - Immunology 2022Quote: ... and Ab49 and stained with fluorescently labelled IgGFab or IgGFc specific F(ab’)2 fragments (DyLight488-conjugated anti-human IgGFc or IgGFab; Jackson ImmunoResearch Laboratory). Samples were mounted on glass slides using Prolong Gold Antifade Mountant with No ...
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bioRxiv - Cell Biology 2020Quote: ... and then probed with anti-α-tubulin (YL1/2 1:10,000) followed by Cy5 anti-rat secondary antibody (Jackson ImmunoResearch, 1:2000). To quantify the tubulin signals ...
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bioRxiv - Immunology 2020Quote: ... The antibody samples were stained with fluorescently labeled IgGFab or IgGFc specific F(ab’)2 fragments (AlexaFluor647-conjugated anti-human IgGFc or IgGFab; Jackson ImmunoResearch Laboratory). Samples were set on glass slides using Prolong™ Gold Antifade Mountant with No ...
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bioRxiv - Neuroscience 2020Quote: ... blocked for 2-3 hours (0.3% Triton X-100, American Bioanalytical, Canton, MA; 3% normal donkey serum, Jackson ImmunoResearch, West Grove, PA), then incubated overnight with primary antibodies (1:1000 + 1% normal donkey serum) ...
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bioRxiv - Neuroscience 2020Quote: ... sections were mounted on microscope gelatin coated slides and incubated for 2 hours in room temperature with cy2 / cy3 conjugated streptavidin (1:1000, Jackson ImmunoResearch Laboratories) in staining solution (1% BSA ...
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bioRxiv - Immunology 2020Quote: ... at 1/1000 dilution and secondary antibody: Peroxidase AffiniPure F(ab’)2 Fragment Donkey Anti-Rabbit IgG (H+L) (Jackson ImmunoResearch Laboratories) horseradish peroxidase conjugated anti-rabbit IgG (Jackson ImmunoResearch ...
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bioRxiv - Neuroscience 2023Quote: ... the cells were incubated with 4’,6-diamidino-2-phenylindol (DAPI; 1 μg/ml) and the corresponding Alexa Fluor-conjugated secondary antibodies (1:500; Jackson ImmunoResearch Laboratories) for 2 h at RT ...
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bioRxiv - Cancer Biology 2023Quote: ... which was detected by the secondary antibody against rabbit IgG (H+L) F(ab’)2 fragment conjugated to Alexa Fluor 647 (Jackson ImmunoResearch Labs) (30 min ...
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bioRxiv - Cell Biology 2023Quote: ... for biotinylated BrdU and (2) non-biotinylated secondary antibodies such as Dylight 488 donkey anti-rabbit (Jackson ImmunoResearch Laboratories, 016-540-084) and Cy3 anti-mouse (Jackson ImmunoResearch Laboratories ...
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bioRxiv - Immunology 2023Quote: ... Samples were then incubated for 2 h with AlexaFluor 647 Goat anti-Rabbit (1:300, Jackson Immunoresearch catalog no. 111-605-144) diluted in 1x PHEM with 0.1% v/v Tween 20 ...
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bioRxiv - Neuroscience 2022Quote: ... and 0.2% PBST (anti-chicken-GFP, Abcam ab6873; anti-mouse-Cy3, Jackson ImmunoResearch 115-165-003; anti-rat-Alexa-fluor-647, Jackson ImmunoResearch 112-605- 167 ...
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bioRxiv - Neuroscience 2024Quote: ... the sections were blocked at room temperature for 90 minutes of incubation with 2% normal donkey serum (Jackson ImmunoResearch, #017-000-121). Next ...
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bioRxiv - Microbiology 2023Quote: ... Cells were washed twice and incubated with Allophycocyanin (APC)-conjugated F(ab′)2 fragment donkey anti-human IgG antibody (1:100 dilution; catalog number 709-136-149; Jackson ImmunoResearch Laboratories) for 30 minutes ...
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bioRxiv - Immunology 2022Quote: ... Proper CAR transduction was checked by flow cytometry by eGFP expression and by using AffiniPure F(ab’)2 Fragment Goat Anti-Human IgG (H+L) (Jackson ImmunoResearch Laboratories).
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bioRxiv - Biophysics 2023Quote: ... anti-mouse Alexa Fluor® 647 and anti-rabbit Alexa Fluor 555 conjugated fluorophore-labeled F(ab)2 donkey secondary antibodies were used (Jackson Immunoresearch). Sections were DAPI counterstained and 3×3 Z-stacked large images (9 mm depth ...
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bioRxiv - Cancer Biology 2022Quote: ... anti-rabbit Rhodamine-Red-X conjugated and anti-mouse Alexa Fluor® 647 conjugated fluorophore-labeled F(ab)2 donkey secondary antibodies were used (Jackson Immunoresearch). Sections were DAPI counterstained and 3×3 Z-stacked large images (9-5 mm depth ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were subsequently stained with F(ab’)2 fragments conjugated with organic fluorophores: AlexaFluor 647 (Thermo Fischer Scientific A37573) anti-mouse IgG (donkey polyclonal, 1:50; Jackson Immunoresearch AB_2340761), and CF680 (Biotium 92139 ...
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bioRxiv - Neuroscience 2024Quote: ... sections were washed in PBS and incubated at room temperature for 2 hours with the donkey anti-chicken secondary antibody conjugated to A594 (1:500; 703-585-155, Jackson ImmunoResearch Laboratories). Afterward ...
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bioRxiv - Microbiology 2024Quote: ... goat anti-rabbit conjugated with horseradish peroxidase (HRP) (1:5,000, 2 hours at room temperature, Jackson ImmunoResearch, catalog no. 115-035- 144), goat anti-mouse conjugated with HRP (1:5,000 ...
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bioRxiv - Neuroscience 2022Quote: ... The sections were first washed in PBS three times for 5 min each and then incubated in blocking solution containing 10% normal donkey serum (017-000-121, Jackson ImmunoResearch, West Grove, PA, USA), 1% bovine serum albumen (A2153 ...
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bioRxiv - Microbiology 2020Quote: ... cells were incubated for 1 hour at room temperature with blocking solution of 5% goat serum in PBS (Jackson ImmunoResearch: catalog no. 005–000-121). To stain for SARS-CoV-2 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... treated with glycine (100 nM in PBS) and blocked with 5 % skim milk powder or 10 % normal goat serum (Jackson ImmunoResearch, West Grove, Pennsylvania, USA) and 1 % BSA in PBST for 1 h at 37 °C ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then washed (3 x 5 min in PBT) and incubated with fluorophore- conjugated streptavidin (Streptavidin AlexaFluor647, Jackson ImmunoResearch, 016-600-084, 1:1000) in 10% NDS in PBT for 2 hours at RT ...
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bioRxiv - Neuroscience 2023Quote: ... sections were washed 4 x 5 min in 0.1 M PB then incubated with a biotinylated secondary antibody (biotinylated donkey anti-mouse, Jackson ImmunoResearch, 715-065-151, 1:500) for 1 hour at RT ...
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bioRxiv - Neuroscience 2022Quote: ... RT) in PBS-T containing 5% NGS at the following concentrations: donkey anti guinea pig DyLight 405 (1:500, Jackson ImmunoResearch, West Grove, PA, USA), goat anti mouse Cy3 (1:500 ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were washed 3×5 min with PBS and stained with 1:1,000 donkey anti-chicken Alexa Fluor 488 secondary antibody (Jackson ImmunoResearch #703-545-155, RRID: AB_2340375), rinsed with PBS ...
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bioRxiv - Neuroscience 2023Quote: ... Cochleas were permeabilized in 0.2% Triton X-100 diluted in 1x PBS for 10 min and blocked in 5% normal donkey serum (Cat# 017-000-121; Jackson ImmunoResearch, West Grove, PA, USA) for 1 hour at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... washed twice in 1XPBS for 5’ each and incubated with Goat anti-Rat-Biotin (1:300, in 1XPBS + 1%NGS, Jackson ImmunoResearch Europe Ltd, Ely, UK) at RT for 1h.
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bioRxiv - Microbiology 2023Quote: ... secondary antibodies were washed three times for 10 mins each in PBS-T and then the slide regions were blocked in 5% normal mouse serum (Jackson ImmunoResearch, West Grove, PA, USA) in PBS-T for 1 hour after which NucBlue Fixed Cell ReadyProbes and Alexa 647 conjugated E-cadherin were applied diluted in PBS-T for 1 hour at room temperature with slides kept dark within the humid slide staining tray ...