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Citations for Jackson ImmunoResearch :
551 - 600 of 857 citations for 5 Bromo 2 bromo difluoro methyl 1H benzimidazole since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... sections were incubated with 0.3% Triton-X in PBS (wash buffer) for 30 minutes for permeabilization and then with 5% Normal goat serum (Jackson ImmunoResearch) in wash buffer (blocking buffer) ...
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bioRxiv - Neuroscience 2022Quote: ... The brain slices were subsequently incubated in blocking buffer (5% donkey serum in TBS-T) containing appropriate fluorescence-conjugated secondary antibodies (1:1000; Jackson ImmunoResearch; Dylight 405-conjugated donkey anti-guinea pig IgG [706-475-148] ...
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bioRxiv - Bioengineering 2022Quote: ... Fixed samples were permeabilized and blocked overnight at 4 °C with a blocking buffer containing 5% IgG-free bovine serum albumin (BSA) (Jackson ImmunoResearch) and 0.3% Triton X-100 (Bio Basic ...
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bioRxiv - Neuroscience 2024Quote: ... pH 7.5) and then incubated with secondary antibody goat anti-mouse or goat anti-rabbit IgG-HRP (1:4,000; Jackson ImmunoResearch Laboratories) in TBS–Tween at room temperature for 2 h ...
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bioRxiv - Neuroscience 2024Quote: ... cells were carefully washed 3×5 min with PBS and incubated with secondary antibodies (Cat.# 711-545-152, 711-605-152, Jackson ImmunoResearch) for 1 h at RT ...
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bioRxiv - Immunology 2023Quote: ... Purified human naïve B cells were cultured in 96-well plates in the presence of AffiniPure F(ab’)₂ Fragment goat anti-human IgG + IgM (5 μg/mL, CAT: 109-006-127, Jackson ImmunoResearch), anti-human CD40 (5 μg/mL ...
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bioRxiv - Biochemistry 2023Quote: ... The nuclei were then quickly washed with assay buffer and resuspended in antibody incubation buffer (assay buffer with 5% NGS (Jackson ImmunoResearch), 0.02% digitonin ...
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bioRxiv - Cell Biology 2023Quote: ... Detection was achieved using secondary antibodies conjugated to horseradish peroxidase (#111-036-003, 106-006-003, 1:5 mL, Jackson ImmunoResearch) and treated with enhanced chemiluminescence (#1705061 ...
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bioRxiv - Biochemistry 2023Quote: ... Triton X-100 in PBS at room temperature for 15 min and washed with the blocking buffer [5% (v/v) normal goat serum (NGS, Jackson ImmunoResearch) and 0.1% (w/v ...
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bioRxiv - Cell Biology 2022Quote: ... Cochleas were permeabilized in 0.2% Triton X-100 in 1x PBS for 10 min and blocked in 5% normal donkey serum (Cat# 017-000-121, Jackson ImmunoResearch) diluted in 1x PBS (blocking buffer ...
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bioRxiv - Cell Biology 2023Quote: ... 1:5) at 4 °C overnight and with the secondary FITC-conjugated or TRITC-conjugated anti-mouse secondary antibody (Jackson ImmunoResearch Laboratories ...
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bioRxiv - Immunology 2023Quote: ... the sections were incubated in Cy-5 anti-rabbit secondary antibody (1:1000, Jackson ImmunoResearch Labs Inc., AB_2340607, West Grove, USA) for 1 hour at 4°C and counterstained with 4’,6-Diamidino-2-Phenylindole ...
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bioRxiv - Neuroscience 2023Quote: ... tissues were rinsed in PBS and blocked in Tris-Buffered Saline (TBS) supplemented with 5 % (vol/vol) Normal Donkey Serum (Jackson Immunoresearch) and 0.3 % (wg/vol ...
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bioRxiv - Immunology 2024Quote: ... diluted in 5% (wt/vol) dried milk in TBS-T and then with Peroxidase-AffiniPure Goat Anti-Rabbit IgG (Jackson ImmunoResearch) at 1 in 5,000 ...
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bioRxiv - Microbiology 2024Quote: ... except that primary antibodies were serially diluted 5-fold and followed by incubation with a 1:3000 dilution of HRP-labeled goat anti-human IgG (Jackson Immunoresearch) in casein for 45 min at 37°C ...
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bioRxiv - Neuroscience 2024Quote: ... the slices were rinsed 3 times for 5 minutes in PBS and incubated in the relevant secondary antibodies mix (donkey anti-sheep Alexa Fluor 405, Jackson Immunoresearch #713-475-003 ...
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Foxn3 is part of a transcriptional network that regulates cilia genes in the developing mouse retinabioRxiv - Developmental Biology 2024Quote: Retina sections were treated with a blocking solution (1% unlabeled adinity-purified Fab fragment donkey anti-mouse IgG (H+L) and 5% donkey serum (Jackson Immunoresearch) in PBS containing 0.1% Triton X-100 (ThermoFisher) ...
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bioRxiv - Developmental Biology 2021Quote: ... MAB8887) and for 2 hours at room temperature with Cy3- or AF488-conjugated secondary antibodies (1:500, Jackson Immunoresearch). For amplified staining ...
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bioRxiv - Developmental Biology 2021Quote: ... and for 2 hours at room temperature with a Cy3-conjugated secondary (1:500, Jackson Immunoresearch, West Grove, PA). For staining on paraffin sections antigen retrieval was performed by either 1mg/ml trypsin (Sigma ...
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bioRxiv - Neuroscience 2021Quote: ... or Cy3-labeled species-specific secondary antibodies for 2 h at a dilution of 1:200 (Invitrogen; Jackson ImmunoResearch). To avoid cross talk between multiple fluorophores ...
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bioRxiv - Biochemistry 2021Quote: ... an Alexa Fluor 647 conjugated donkey anti-human IgG Fc F(ab’)2 fragment (Jackson ImmunoResearch, West Grove, PA) was used as secondary antibody at 5 μg/ml concentration ...
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bioRxiv - Biochemistry 2022Quote: ... an Alexa Fluor 647 conjugated donkey anti-human IgG Fc F(ab’)2 fragment (Jackson ImmunoResearch, West Grove, PA) was used as secondary antibody at 5 μg/ml concentration ...
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bioRxiv - Cell Biology 2022Quote: ... CyTM3 AffiniPure F (ab’)2 Fragment Donkey Anti-Rabbit IgG (H + L) (1: 200, Jackson ImmunoResearch, Cat No: 711166152) and DyLight 488 Horse Anti-Mouse IgG Antibody (1 ...
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bioRxiv - Biochemistry 2021Quote: ... an Alexa Fluor 647 conjugated donkey anti-human IgG Fc F(ab’)2 fragment (Jackson ImmunoResearch, West Grove, PA) was used as secondary antibody at 5 µg/ml concentration ...
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bioRxiv - Immunology 2023Quote: ... CAR-T cells were stained with anti-mouse IgG F(ab’)2 (FITC, Jackson ImmunoResearch, Cat#515-095-072) and sorted out for expansion with 150 IU/mL of IL-2 in the presence of Tazemetostat (1.0 uM ...
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bioRxiv - Immunology 2023Quote: ... Cells were then stimulated with nicotine for 20 min and subsequently anti-mouse IgM F(ab’)2 (Jackson ImmunoResearch) for 2 min (pCD19 ...
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bioRxiv - Microbiology 2022Quote: ... an Alexa Fluor 647 conjugated donkey anti-human IgG Fc F(ab’)2 fragment (Jackson ImmunoResearch, West Grove, PA) was used as secondary antibody at 5 μg/ml concentration ...
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bioRxiv - Biochemistry 2022Quote: ... An Alexa Fluor 647 conjugated donkey anti-human IgG Fc F(ab’)2 fragment (Jackson ImmunoResearch, West Grove, PA) was used as secondary antibody at the concentration of 5 μg/ml ...
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bioRxiv - Microbiology 2024Quote: ... followed by 30 min incubation with 1:3000 human secondary F(ab′)2 Goat Anti-Human IgG (Jackson ImmunoResearch) and fluorescence measurement on a Bio-Plex 2000 system ...
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bioRxiv - Neuroscience 2024Quote: ... anti-Iba1 (1:1000) in PBS with 0.3% Triton X-100 (MilliporeSigma) and 2% normal donkey serum (Jackson ImmunoResearch Laboratories ...
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bioRxiv - Immunology 2024Quote: ... Cells were washed again with TBS before blocking with TBS containing 2% BSA (Cat. 001-000-182, Jackson ImmunoResearch) for 60 min at room temperature ...
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bioRxiv - Immunology 2024Quote: ... B cells were stimulated with 10 μg/mL F(ab’)2 goat anti-mouse IgM (anti-μ) (Jackson ImmunoResearch). At the indicated times ...
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bioRxiv - Neuroscience 2024Quote: ... 2–3 hours in secondary antibody 1:500 anti-chicken-IgY Alexa488-conjugated (Jackson ImmunoResearch cat# 703-545-155) in blocking solution ...
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bioRxiv - Cell Biology 2024Quote: ... the sections were incubated at room temperature for 2 hours in the corresponding secondary gold-conjugated antibodies (Jackson ImmunoResearch) diluted in PBS + 1.2% gelatin ...
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bioRxiv - Developmental Biology 2024Quote: ... Samples were washed in PBST and blocked with 2% normal donkey serum (Jackson ImmunoResearch Laboratories, Inc. 017-000-121) in PBST ...
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bioRxiv - Cell Biology 2020Quote: ... Blots were washed 3 x 5 mins in 1X TBST and incubated in 5% non-fat dry milk in 1X TBST containing anti-rabbit (H+L) horseradish peroxidase (Jackson ImmunoResearch Laboratories) secondary antibodies at a dilution of 1:20,000 for 1 hour at room temp ...
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bioRxiv - Cell Biology 2020Quote: ... Fc receptors were blocked by incubating cells in PBS supplemented with 5% FCS with 250 µ g.ml−1 purified mouse IgG (Jackson ImmunoResearch Laboratories). Antibody stainings were performed in PBS/5% FCS on ice for 30 minutes with optimal dilutions of commercially-prepared antibodies ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were washed 3 times for 5 minutes each with PBT (0.5% Triton X-100 in PBS) and incubated with the corresponding secondary antibodies Alexa Fluor 488 (Anti-Chicken IgG, Jackson ImmunoResearch Labs) and Cy3 (Anti-Rabbit IgG ...
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bioRxiv - Immunology 2020Quote: ... CAR detection was performed using anti-Fab specific antibody at day 5 post-transduction (109-606-006, Jackson ImmunoResearch, West Grove, PA).
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bioRxiv - Immunology 2021Quote: ... Plated peptides were incubated with the individual monoclonal antibodies (5 µg/ml diluted in blocking buffer) and further incubated with donkey anti-human alkaline phosphatase-conjugated secondary antibody (Jackson ImmunoResearch, USA). Immune complexes were identified following incubation with SIGMAFAST™ PNPP (SIGMA ...
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bioRxiv - Cell Biology 2021Quote: ... membrane was incubated with secondary Goat anti-Chicken HRP (1:5000 in 5%BSA/1X PBS-T, Jackson ImmunoResearch, 103-035-155) then incubated for 2 min in the dark with ECL Western Blotting Substrate (ThermoFisher ...
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bioRxiv - Cell Biology 2021Quote: ... it was incubated for 30 min in PBS with 5% non-fat milk and then for 1h at RT with horseradish peroxidase-conjugated secondary anti-mouse antibody (1:10000 dilution in PBS with 5% non-fat milk, Jackson Immunoresearch Laboratories), and washed again ...
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bioRxiv - Physiology 2023Quote: ... slices were washed 3×5 min in PBS 0.01M at RT – 4 mL/well and incubated in donkey anti-guinea pig-AF647 (Jackson ImmunoResearch, 1:500) diluted in [PBS 0.01M + 0.3% Triton] ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were subsequently washed four times for 5 minutes in TBS-T before secondary antibody (Jackson ImmunoResearch, 711-035-152, 1:15,000) was added for 1 hour at room temperature in 5% skim milk ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were permeabilized and blocked for 1–2 h at room temperature (RT) in 1X PBS with 0.5% Triton-X (0.5% PBST) and 5–10% normal donkey serum (NDS, Jackson ImmunoResearch 017-000-121). Primary and secondary antibodies were diluted in 0.5% PBST and 5% NDS ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were washed 3X using TBST-High Salt for 5 minutes on a rocking surface at room temperature before being incubated with secondary antibodies conjugated with Horseradish Peroxidase (Jackson ImmunoResearch Labs) for 45 minutes in 5% Non-fat dry milk rocking at room temperature ...
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bioRxiv - Immunology 2023Quote: ... before cultured in complete RPMI-1640 media without/with 5 μg/mL goat anti-mouse IgM (Jackson ImmunoResearch; cat # 115-006-075) and/or recombinant murine 25 ng/ml IL-4 (Peprotech ...
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bioRxiv - Developmental Biology 2023Quote: ... Samples were permeabilized and blocked in a single step with 0.2% Triton-X and 5% donkey serum (Jackson Immunoresearch, 017-000-121). Primary antibodies were subsequently added and incubated overnight at 4 °C ...
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bioRxiv - Microbiology 2023Quote: ... Slide regions were washed in PBS for 5 mins and then blocked with 10% normal goat (Jackson ImmunoResearch, West Grove, PA, USA) or donkey serum (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2023Quote: ... washed in PBST 3 times (5 min in each time) and incubated with secondary antibodies (mouse anti-Cy3, Jackson Immunoresearch,1:300) for 1h ...