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Citations for Jackson ImmunoResearch :
651 - 700 of 726 citations for 3' Chloro 3 3 chloro 5 fluorophenyl 4' fluoropropiophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated overnight at 4°C in primary antibody solution containing 2% Normal Donkey Serum (NDS; Jackson Immunoresearch Laboratories Inc. 017-000-121) + 1xPBS + 0.03% Triton + primary antibody (rabbit anti-P2RY12 ...
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bioRxiv - Cell Biology 2020Quote: ... Blots were washed 3 x 5 mins in 1X TBST and incubated in 5% non-fat dry milk in 1X TBST containing anti-rabbit (H+L) horseradish peroxidase (Jackson ImmunoResearch Laboratories) secondary antibodies at a dilution of 1:20,000 for 1 hour at room temp ...
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bioRxiv - Cell Biology 2020Quote: ... Fc receptors were blocked by incubating cells in PBS supplemented with 5% FCS with 250 µ g.ml−1 purified mouse IgG (Jackson ImmunoResearch Laboratories). Antibody stainings were performed in PBS/5% FCS on ice for 30 minutes with optimal dilutions of commercially-prepared antibodies ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were washed 3 times for 5 minutes each with PBT (0.5% Triton X-100 in PBS) and incubated with the corresponding secondary antibodies Alexa Fluor 488 (Anti-Chicken IgG, Jackson ImmunoResearch Labs) and Cy3 (Anti-Rabbit IgG ...
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bioRxiv - Immunology 2020Quote: ... CAR detection was performed using anti-Fab specific antibody at day 5 post-transduction (109-606-006, Jackson ImmunoResearch, West Grove, PA).
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bioRxiv - Immunology 2021Quote: ... Plated peptides were incubated with the individual monoclonal antibodies (5 µg/ml diluted in blocking buffer) and further incubated with donkey anti-human alkaline phosphatase-conjugated secondary antibody (Jackson ImmunoResearch, USA). Immune complexes were identified following incubation with SIGMAFAST™ PNPP (SIGMA ...
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bioRxiv - Cell Biology 2021Quote: ... membrane was incubated with secondary Goat anti-Chicken HRP (1:5000 in 5%BSA/1X PBS-T, Jackson ImmunoResearch, 103-035-155) then incubated for 2 min in the dark with ECL Western Blotting Substrate (ThermoFisher ...
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bioRxiv - Cell Biology 2021Quote: ... it was incubated for 30 min in PBS with 5% non-fat milk and then for 1h at RT with horseradish peroxidase-conjugated secondary anti-mouse antibody (1:10000 dilution in PBS with 5% non-fat milk, Jackson Immunoresearch Laboratories), and washed again ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were subsequently washed four times for 5 minutes in TBS-T before secondary antibody (Jackson ImmunoResearch, 711-035-152, 1:15,000) was added for 1 hour at room temperature in 5% skim milk ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were permeabilized and blocked for 1–2 h at room temperature (RT) in 1X PBS with 0.5% Triton-X (0.5% PBST) and 5–10% normal donkey serum (NDS, Jackson ImmunoResearch 017-000-121). Primary and secondary antibodies were diluted in 0.5% PBST and 5% NDS ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were washed 3X using TBST-High Salt for 5 minutes on a rocking surface at room temperature before being incubated with secondary antibodies conjugated with Horseradish Peroxidase (Jackson ImmunoResearch Labs) for 45 minutes in 5% Non-fat dry milk rocking at room temperature ...
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bioRxiv - Immunology 2023Quote: ... before cultured in complete RPMI-1640 media without/with 5 μg/mL goat anti-mouse IgM (Jackson ImmunoResearch; cat # 115-006-075) and/or recombinant murine 25 ng/ml IL-4 (Peprotech ...
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bioRxiv - Developmental Biology 2023Quote: ... Samples were permeabilized and blocked in a single step with 0.2% Triton-X and 5% donkey serum (Jackson Immunoresearch, 017-000-121). Primary antibodies were subsequently added and incubated overnight at 4 °C ...
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bioRxiv - Microbiology 2023Quote: ... Slide regions were washed in PBS for 5 mins and then blocked with 10% normal goat (Jackson ImmunoResearch, West Grove, PA, USA) or donkey serum (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2024Quote: Samples were washed twice for 10 minutes with PBS-T (0.5% Triton-X100 in PBS) and incubated with Cy3 and/or Alexa Fluor 647-conjugated secondary antibody (1:500, Jackson ImmunoResearch Laboratories), DAPI (4’,6-Diamidino-2-Phenylindole ...
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bioRxiv - Immunology 2021Quote: ... for 30 minutes at 4°C and subsequently labeled with Rhodamine conjugated anti-mouse IgG (Jackson ImmunoResearch, Cat#115-026-062, 1:100 in PBS). Fluorescently labeled nuclei were counted using a Nikon Eclipse TE2000-U at 20x magnification.
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bioRxiv - Neuroscience 2021Quote: ... above tissue sections under ISH were incubated with chicken anti-NeuN antibody (1:200; Aves, Davis, CA) overnight at 4°C followed by donkey anti-chicken IgG Alexa 488 (Jackson ImmunoResearch labs, West Grove, PA) for 1 hr at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were rinsed in PBS and incubated at 4°C in PBS containing 2% donkey serum and secondary antibodies conjugated to anti-rabbit Cy3 (Jackson ImmunoResearch Inc., West Grove, PA) and anti-guinea pig CF405M (Biotium ...
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bioRxiv - Cell Biology 2020Quote: ... Blotted proteins were incubated with corresponding primary antibodies at 4°C overnight followed by incubation with horseradish peroxidase conjugated secondary antibodies (Jackson ImmunoResearch, West Grove, PA, USA). Amersham ECL Western blotting detection reagent (GE Healthcare Life Sciences ...
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bioRxiv - Neuroscience 2022Quote: ... Cat#ACC-102) over night at 4°C followed by 1 hour probing with donkey anti-rabbit HRP (1:10,000; Jackson ImmunoResearch Labs Cat#711-035-152). The membrane were imaged via enhanced chemiluminescence photodetection with the BioRad ChemiDoc XRS+ system.
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bioRxiv - Cancer Biology 2023Quote: ... for 10 minutes and washed 3 times in PBS for 5 minutes at room temperature (RT), followed by incubation for 30 minutes in blocking buffer (1% IgG-free BSA, 4% donkey serum (Jackson ImmunoResearch, West Grove, PA, USA) in PBS) ...
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bioRxiv - Neuroscience 2024Quote: ... and donkey anti-guinea pig Alexa Fluor (AF) 488 (Catalog #:706-545-148) had a 1:500 dilution and were stored overnight at 4 °C (Jackson ImmunoResearch; West Grove, PA, USA). Sections were then mounted and cover-slipped with using 50% glycerol in 0.02M KPBS and clear nail polish was used to seal the coverslip onto the slide.
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bioRxiv - Neuroscience 2024Quote: ... Membranes were incubated overnight at 4°C with the primary antibodies and then with the corresponding horseradish peroxidase-conjugated secondary antibodies (Jackson Immunoresearch, West Grove, PA, USA). All membranes were revealed using ECL chemiluminescence kit according to the manufacturer’s instructions (GE Healthcare ...
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bioRxiv - Immunology 2020Quote: ... circumscribed with ImmEdge pen (Vector) and blocked in TBST (100mL Tris-HCl, 150mM NaCl, 0.05% Tween-20) containing 5% Donkey serum (Jackson ImmunoResearch #017-000-121) for 2 hours at RT ...
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bioRxiv - Genetics 2021Quote: ... The fixed tissues were then subjected to three rounds of 15 minute-each wash with 0.1% PBST (0.1% Triton X-100 in PBS) and blocked with 5% donkey serum (Jackson ImmunoResearch Laboratories, 107-000-121) for 1 hour at room temperature followed by incubation with primary antibodies in 5% donkey serum at 4°C ...
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bioRxiv - Immunology 2022Quote: ... cells were incubated for 1h at RT with blocking solution of 5% goat serum in PBS (Jackson ImmunoResearch, catalog no. 005–000-121). A rabbit polyclonal anti-SARS-CoV-2 nucleocapsid antibody (GeneTex ...
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bioRxiv - Neuroscience 2021Quote: ... The membranes were blocked in 5% w/v bovine serum albumin (IgG-free and protease-free BSA, Jackson ImmunoResearch Laboratories, West Grove, PA) in 50 mM Tris-HCl (pH 7.4) ...
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bioRxiv - Cancer Biology 2022Quote: ... Sections were washed 3 times for 5 minutes each in PBS+0.3% TritonX-100 and incubated with appropriate fluorescent conjugated secondary antibody (Jackson ImmunoResearch, West Grove, PA) for 45 minutes at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... Antibodies for O1 and O4 were diluted in N2B27 base medium supplemented with 5% Donkey Serum (v/v) (Jackson ImmunoResearch, 017-000-121) and added to wells for 18 minutes at 37°C ...
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bioRxiv - Developmental Biology 2024Quote: ... Sections of 20 um thickness were blocked in PBS + 0.3% Triton X-100 and 5% normal donkey serum (Jackson ImmunoResearch, 017-000-121) for one hour ...
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bioRxiv - Cell Biology 2024Quote: ... 0.1 M glycine, 0.1% Triton-X, syringe filtered) for 10 min, then blocking buffer (1X PBS, 5% goat serum (Jackson ImmunoResearch; 005-000-121), 2% bovine serum albumin (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2022Quote: ... Pre-prepared immunopanning dishes were setup as follows: a 10cm petri dish was incubated at 4°C overnight with 30ul anti-rat antibody (Jackson ImmunoResearch Laboratories, Inc, 112-005-167) in 10 ml 50 mM Tris-HCL (pH 9.5) ...
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bioRxiv - Physiology 2023Quote: ... Cells were then washed and incubated with primary antibodies diluted in blocking solution overnight at 4 °C followed by incubation with an AlexaFluor 488-conjugated AffiniPure Donkey Anti-Rabbit IgG secondary antibody (Jackson ImmunoResearch, 711-545-152, 1:500) for 1h at RT ...
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bioRxiv - Cell Biology 2023Quote: ... cells were fixed with 4% paraformaldehyde and immunofluorescence was performed as described previously [54] using appropriate primary and secondary antibodies (Jackson ImmunoResearch Laboratories, West Grove, PA, USA), and coverslips were mounted using Prolong Gold Antifade Reagent with 4′,6-diamidino-2-phenylindole (DAPI ...
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Differential impact of a dyskeratosis congenita mutation in TPP1 on mouse hematopoiesis and germlinebioRxiv - Cell Biology 2021Quote: ... deparaffinized sections were boiled in 0.1 M Sodium Citrate for 10 minutes for antigen retrieval and sections were blocked in suppressor serum (5% goat serum (Jackson ImmunoResearch #005-000-1210), 95% blocking solution (3% BSA and 0.5% Tween in PBS) ...
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bioRxiv - Cancer Biology 2022Quote: ... Coverslips were washed with PBS 1X three times for 5 min at RT and incubated with Donkey anti-mouse IgG Alexa Fluor 488 secondary antibody (1:200; Jackson ImmunoResearch, 715-095-150) and Rhodamine-conjugated Phalloidin (Sigma ...
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bioRxiv - Developmental Biology 2022Quote: ... permeabilized with 0.1% Triton X-100 in PBS (PBST) for 30 min and blocked with 5% normal donkey serum (Jackson ImmunoResearch Laboratories, #017-000-121) diluted in PBST (blocking solution ...
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bioRxiv - Immunology 2020Quote: ... plates were washed 5 times with 0.05% PBST and then incubated with horseradish peroxidase (HRP)-conjugated anti-mouse IgG (Jackson ImmunoResearch Laboratories 115-035-003), IgG1 (Jackson ImmunoResearch Laboratories 115-035-205) ...
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bioRxiv - Microbiology 2022Quote: ... The grids were washed on three drops of PBS before being incubated for 12 min with a 5 nm gold-labelled anti-rabbit secondary antibody (Jackson ImmunoResearch West Grove, PA) at dilutions of 1:10 ...
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bioRxiv - Molecular Biology 2023Quote: ... After washing once in PBS-T (PBS with 0.2% Tween-20), cells were blocked with blocking buffer (PBS-T, 2% BSA, and 5% goat serum (Jackson Immunoresearch/Dianova, 017-000-121) for 1 hour at room temperature ...
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bioRxiv - Developmental Biology 2024Quote: P5-6 retinas were incubated in wash buffer (PBS with 0.1%Triton X100) with gentle rocking for 15 min and then in blocking buffer (wash buffer with 5% normal donkey serum (Jackson ImmunoResearch, 017-000-121)) for 1-2 hrs ...
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bioRxiv - Immunology 2024Quote: ... or cultured cells stained for the expression of surface markers with specific antibodies in PBS with 5% FBS and 20% normal goat serum (Jackson ImmunoResearch 005-000-121) and 0.01% NaN3 for 20 min on ice ...
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bioRxiv - Neuroscience 2023Quote: ... were washed in 1X TBS three times and then blocked and permeabilized for one hour in 1X TBS containing 5% Normal donkey serum (NDS, Jackson ImmunoResearch Laboratories, Lot #161590) and 0.5% Triton x-100 ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were blocked for 1 hour with block buffer (0.05% IGEPAL CA-630, 0.05% Triton X-100, 5% goat normal serum [Jackson ImmunoResearch; 005-000-121] in 1xPBS). Samples were incubated with primaries/nanobodies diluted in block buffer for 16 hours at 4 °C on a rocker ...
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bioRxiv - Developmental Biology 2023Quote: ... The cells were then washed with PBS three times (5 minutes each) and incubated with Alexa Fluor 488 anti-mouse secondary antibody (1:1000, Jackson Immunoresearch #115-545-146) at 4°C overnight ...
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bioRxiv - Developmental Biology 2023Quote: ... After washing once in PBS-T (PBS with 0.2% Tween-20), cells were blocked with blocking buffer (PBS-T, 2% BSA and 5% goat serum (Jackson Immunoresearch/Dianova, 017-000-121) for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... the tissue was washed six times in PBS (5 min each) then incubated with a biotinylated goat anti-rabbit antibody (1:500; Jackson ImmunoResearch Laboratories; RRID: AB_2337965) prepared in NDS for 1 hr (RT) ...
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bioRxiv - Developmental Biology 2023Quote: ... Secondary antibody incubation was performed in 5% milk for 1 hour at room temperature using 1:5000 for anti-rabbit-HRP (Jackson Immunoresearch, 711-035-152) or anti-mouse-HRP (Jackson Immunoresearch ...
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bioRxiv - Bioengineering 2024Quote: ... The cells were washed with 1X DPBS and then incubated in blocking buffer (consisting of 5% (v/v) normal donkey serum (Jackson ImmunoResearch, West Grove, PA) and 1% (w/v ...
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bioRxiv - Cell Biology 2024Quote: ... The following secondary antibodies were used at 1:10,000 dilution and incubated for 1 hour at room temperature in 5% milk w/ TBST: HRP donkey anti-rabbit (Jackson ImmunoResearch #711-035-152) and HRP goat anti-mouse IgG subclass 1-specific (Jackson ImmunoResearch #115-035-205) ...