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Citations for Jackson ImmunoResearch :
551 - 600 of 726 citations for 3' Chloro 3 3 chloro 5 fluorophenyl 4' fluoropropiophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... cells were washed with PBS and blocked with 2% BSA (w/v) (Roth) and 4% donkey serum (v/v) (Jackson Immunoresearch Laboratories) in PBS for 30 min at RT ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were rinsed three times in PBS and incubated for 60–90 min at room temperature or overnight at 4°C with the Alexa Fluor 488- and 594 and 647-conjugated secondary antibodies (1:500; Thermo Fisher Science or Jackson ImmunoResearch).
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bioRxiv - Immunology 2020Quote: ... Membranes were incubated in primary antibodies for 1-3 hours at room temperature or overnight at 4°C followed by incubation in 20 ng/ml secondary horseradish-peroxidase (HRP)-conjugated secondary antibodies (Jackson ImmunoResearch Laboratories) for 45 min at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... the cells were incubated with 4’,6-diamidino-2-phenylindol (DAPI; 1 μg/ml) and the corresponding Alexa Fluor-conjugated secondary antibodies (1:500; Jackson ImmunoResearch Laboratories) for 2 h at RT ...
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bioRxiv - Neuroscience 2024Quote: ... primary antibody overnight at 4°C and secondary antibody (anti-rabbit-Alexa Fluor 647, 1:400, Jackson ImmunoResearch, Inc., West Grove, PA) for 3-4 hours in the dark ...
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bioRxiv - Neuroscience 2023Quote: ... followed by a 4 h incubation with a secondary donkey anti-guinea pig IgG [F(ab’)2fragments] (1:500, Jackson ImmunoResearch #706606148). Tissues were rinsed and mounted using Prolong diamond antifade (Life Technologies) ...
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bioRxiv - Physiology 2024Quote: ... treated with 1M HCl for 1 hour and incubated overnight at 4°C with anti-BrdU antibody following incubation with Alexa Fluor-488 conjugated goat anti-mouse IgG (Jackson ImmunoResearch Laboratories). Slides were mounted in mounting medium for fluorescence with DAPI (Vector Laboratories) ...
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bioRxiv - Biophysics 2024Quote: ... Nonspecific cross-reactivity was blocked by incubating the samples for 4 h in 1% BSA/PBS (Jackson ImmunoResearch, West Grove, PA, USA). The cells were then incubated with primary antibodies anti-RyR (IgG1 ...
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bioRxiv - Cell Biology 2020Quote: ... The membrane was rinsed 5 x with TBST and incubated in TBST with goat secondary antibodies coupled to HRP (1:10’000; Jackson ImmunoResearch) for 1 h at room temperature ...
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bioRxiv - Developmental Biology 2021Quote: ... Tissue was circled with a pap pen and after washing three times with PBS was blocked in in PBS with 0.3% Triton X-100 and 5% normal donkey serum (017-000-121, Jackson ImmunoResearch). After blocking ...
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bioRxiv - Microbiology 2021Quote: ... After fixation the cells were permeabilized with 0.5% Triton X-100 in PBS and blocked in 5% (v/v) normal donkey serum (Jackson Immunoresearch). Cells were incubated with primary antibodies at 37°C for 1 hour ...
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bioRxiv - Bioengineering 2020Quote: ... and resuspension for FACS was performed in a FACS buffer solution containing 5% goat serum (Jackson Immunoresearch #005-000-121) and 1 mM EDTA in 1× phosphate buffer saline (PBS) ...
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bioRxiv - Neuroscience 2020Quote: ... Coverslips were incubated in blocking solution (10% IgG-Free BSA, 5% donkey serum or goat serum in PBS, Jackson ImmunoResearch) for 30 minutes at room temperature (RT) ...
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bioRxiv - Cell Biology 2021Quote: ... The membrane was rinsed 5 x with TBST and incubated in TBST/5 % dry milk with goat secondary antibodies coupled to HRP (1:10’000; Jackson ImmunoResearch) for 1 h at room temperature ...
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bioRxiv - Cancer Biology 2020Quote: ... plates were incubated with peroxidase conjugated goat anti-human IgG secondary (1/10,000 in 5% non-fat milk in PBS; Jackson ImmunoResearch) for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... followed by three washes in PBS for 5 min and incubation with secondary antibodies (fluorophore-coupled donkey antibodies, Jackson ImmunoResearch) in blocking solution at RT for 30 to 60 min ...
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bioRxiv - Cell Biology 2021Quote: ... Antibodies were then crosslinked with donkey anti-mouse IgG (5 μg/ml, 715-005-150, Jackson Immunoresearch, West Grove, PA) on ice for another 10 min ...
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bioRxiv - Cell Biology 2022Quote: ... Sections were permeabilized with 0.5% Triton X-100/PBS and then blocked with 5% goat serum (Jackson ImmunoResearch Laboratories, USA) for 1 hour at room temperature ...
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bioRxiv - Bioengineering 2022Quote: ... Non-specific immunoreactivity was suppressed by incubating the sections in a mixture of 5% normal donkey serum (NDS; Jackson ImmunoResearch) and 0.3% Triton X-100 (Sigma ...
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bioRxiv - Neuroscience 2020Quote: ... (5) cultures were incubated in Cy3-AffiniPure Donkey Anti-Rabbit IgG (H+L) (1:500, Jackson ImmunoResearch, 711-165-152) and Alexa Fluor 488 AffiniPure Donkey Anti-Chicken IgG (H+L ...
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bioRxiv - Immunology 2022Quote: ... incubated in 0.1 M glycine for 10 min at room temperature and subsequently incubated in 5% goat serum (Jackson ImmunoResearch) for 20 minutes at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... the slides were washed briefly with PBS followed by permeabilization and blocking with 0.3% TritonX-100 and 5% Normal Donkey Serum (NDS, Jackson ImmunoResearch) in PBS for 1 hour at RT ...
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bioRxiv - Neuroscience 2021Quote: ... and then blocked for 1 hour at room temperature in 5% normal donkey serum (Jackson ImmunoResearch, West Grove, PA, USA)/0.5% TTBS ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were blocked for 2 hours at room temperature (RT) in 5% normal goat serum (Jackson ImmunoResearch, West Grove, PA). Cells were incubated in one of the following primary antibodies in blocking solution containing 5% normal serum at 4°C overnight ...
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bioRxiv - Immunology 2023Quote: ... Sections were washed in PBS and blocked in PBS containing 0.1% Triton X-100 and 5% normal donkey serum (NDS) (Jackson ImmunoResearch) for 45 minutes at room temperature ...
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bioRxiv - Immunology 2023Quote: ... and blocked with Image-iT FX Signal Enhancer (Cell Signal) followed by PBS containing 0.1% (v/v) Tween20 and 5% (v/v) normal goat serum (Jackson Immunoresearch). Samples were then stained overnight at 4C with anti-CD4 (AlexaFluor488 ...
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bioRxiv - Cancer Biology 2023Quote: ... and incubated with blocking buffer (5% normal donkey serum [Jackson ImmunoResearch] in 0.1% Triton X-100 in PBS [PBS-Tx]) for 1 hour at RT ...
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bioRxiv - Cancer Biology 2024Quote: ... 10 µm cryosections were blocked in PBS with 0.3% Triton X-100 and 5% normal donkey serum (017-000-121, Jackson ImmunoResearch) and incubated overnight in a humidified chamber at 4 °C with primary antibodies diluted in PBS with 0.3% Triton X-100 and raised against ...
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bioRxiv - Developmental Biology 2023Quote: ... frozen sections at 10-μm thickness were blocked in PBS with 0.3% Triton X-100 and 5% normal donkey serum (017-000-121, Jackson ImmunoResearch) and then incubated with primary antibodies diluted in PBS with 0.3% Triton X-100 at 4°C overnight in a humidified chamber ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples were incubated in blocking solution containing 5% normal donkey serum (NDS) (Jackson Immunoresearch, West Grove, PA, USA; Cat#NC9624464) and 0.2% Triton-X 100 in 1x PBS for 1 hour and then incubated in primary antibodies with 2.5% NDS and 0.1% Triton-X 100 in 1x PBS either for 2 hours at room temperature (RT ...
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bioRxiv - Neuroscience 2024Quote: ... followed by blocking for 1□h with 0.2% Triton X-100 and 5% normal goat serum (Jackson ImmunoResearch, Suffolk, UK) in PBS ...
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bioRxiv - Immunology 2024Quote: ... Fixed and permeabilized cells were then washed 3x with PBS, and blocked (PBS, 0.3% Triton X-100, and 5% normal human serum (Jackson ImmunoResearch)) for 1h at room temperature ...
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bioRxiv - Immunology 2024Quote: ... and specified combinations of 5 µg/mL (unless otherwise stated) goat anti-mouse IgM Fab2 (Jackson Immunoresearch 115-006-075), 1 µg/mL anti-CD40 (bioxcell BP0016-2) ...
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bioRxiv - Neuroscience 2024Quote: ... cells were then permeabilised with 0.1% Triton X-100 for 10 minutes and then blocked in 5% goat serum (diluted in 0.01M PBS: block, Jackson ImmunoResearch) for 1 hr ...
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bioRxiv - Developmental Biology 2024Quote: ... Slides were washed with PBS three times at room temperature and were blocked in PBS 0.3% Triton X-100 (PBST) with 5% donkey serum (017-000-121, Jackson ImmunoResearch). Primary antibodies were diluted in PBST and incubated overnight at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... animals were incubated overnight at 4°C in Alexa Fluor 488 donkey anti-chicken secondary antibody (1:1000, Jackson ImmunoResearch, 703-545-155), Alexa Fluor 594 goat anti-mouse secondary antibody (1:1000 ...
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bioRxiv - Microbiology 2020Quote: ... Cells are incubated with mAb 4G2 (1 µg/mL) for 1hour at 4 °C followed by secondary anti-mouse Alexa 488 (Jackson Immunoresearch, 1:50000). After washing with PBS ...
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bioRxiv - Neuroscience 2021Quote: ... overnight at 4°C followed by the addition of a goat anti-rat HRP-conjugated secondary antibody (Jackson ImmunoResearch, Westgrove, PA; 1:10,000) for 1 hr ...
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bioRxiv - Neuroscience 2022Quote: ... 0.2% Triton X-100 and incubated overnight at 4°C with a CY5-conjugated secondary antibody (1:400, goat anti-mouse, Jackson Immunoresearch, Suffolk, UK), washed in 1x PBS and embedded in 1x PBS ...
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bioRxiv - Microbiology 2021Quote: ... Labeled cells were washed with ice-cold PBS-BSA followed by secondary labeling for 1 h at 4°C in 2.5 mL 1:200 PE-conjugated goat anti-human-IgG (Jackson ImmunoResearch 109-115-098) to label for bound monoclonal antibody or 1:200 Alexa-647-conjugated goat anti-human-IgA+IgG+IgM (Jackson ImmunoResearch 109-605-064 ...
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bioRxiv - Microbiology 2021Quote: ... samples were further statically incubated for 1 h at 4°C with APC-conjugated polyclonal goat-anti-human IgG F(ab’)2 antibody (Jackson immunoresearch, 1:500). Fab fragments were detected with Alexa Fluor 647 conjugated goat-anti-human-kappa F(ab’)2 antibody (Southern Biotech ...
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bioRxiv - Microbiology 2020Quote: ... Labeled cells were washed with ice-cold PBS-BSA followed by secondary labeling for 1 h at 4°C in 2.5 mL 1:200 PE-conjugated goat anti-human-IgG (Jackson ImmunoResearch 109-115-098) to label for bound antibody ...
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bioRxiv - Cell Biology 2021Quote: ... Membranes were incubated with primary antibodies (4°C overinight) and then secondary peroxidase-conjugated Goat Anti-Rabbit IgG (Jackson ImmunoResearch 111-035-144) or peroxidase-conjugated Goat Anti-Mouse IgG (Jackson Immuno Research 115-035-174 ...
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bioRxiv - Cell Biology 2023Quote: ... at 4°C for 1 h following incubation with fluorescein (FITC) AffiniPure goat anti-rat IgM (1:100, Jackson ImmunoResearch, 112-095-075) or allophycocyanin-conjugated (APC ...
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bioRxiv - Cell Biology 2022Quote: ... 4 µg/ml) and donkey (711-166-152, 711-546-152, 715-166-150 or 715-546-151, Jackson ImmunoResearch, 1.5 µg/ml) secondary antibodies ...
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bioRxiv - Biochemistry 2024Quote: ... cells were washed once with FACS buffer and were labelled for 30 minutes at 4 °C with either Biotin-SP-conjugated AffiniPure Donkey Anti-Mouse IgG (H+L) (Jackson Immunoresearch Laboratories, Inc.) for samples 1 and 2 or with FACS buffer for sample 3 ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were then permeabilized in IF buffer (PBS supplemented with 0.1% Triton X-100, 5% normal donkey serum (017-000-121; Jackson ImmunoResearch Laboratories), and 3% bovine serum albumin (BP1605-100 ...
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bioRxiv - Neuroscience 2021Quote: ... and incubated with secondary antibodies conjugated to Alexa Fluor 488 and 647 (1:250 in 5% normal goat serum; Jackson ImmunoResearch). To visualize biotinylated proteins ...
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bioRxiv - Neuroscience 2021Quote: The brain sections undergoing immunohistochemical staining were washed in PBS 3 times (10 minutes each time) and incubated in a blocking solution containing 0.3% TritonX-100 and 5% normal donkey serum (Jackson ImmunoResearch, USA) in PBS for 1 hour at 37°C ...
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bioRxiv - Immunology 2021Quote: ... cells were incubated for 1 hour at 37°C with blocking solution of 5% goat serum in PBS (catalog no. 005-000-121; Jackson ImmunoResearch). A rabbit polyclonal anti-SARS-CoV-2 nucleocapsid antibody (catalog no ...