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Citations for Jackson ImmunoResearch :
501 - 550 of 569 citations for 6 Hydroxymethyl 4 methoxy 5 methyl nicotinic acid methyl ester since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... for 30 minutes at 4°C and subsequently labeled with Rhodamine conjugated anti-mouse IgG (Jackson ImmunoResearch, Cat#115-026-062, 1:100 in PBS). Fluorescently labeled nuclei were counted using a Nikon Eclipse TE2000-U at 20x magnification.
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bioRxiv - Neuroscience 2021Quote: ... above tissue sections under ISH were incubated with chicken anti-NeuN antibody (1:200; Aves, Davis, CA) overnight at 4°C followed by donkey anti-chicken IgG Alexa 488 (Jackson ImmunoResearch labs, West Grove, PA) for 1 hr at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were rinsed in PBS and incubated at 4°C in PBS containing 2% donkey serum and secondary antibodies conjugated to anti-rabbit Cy3 (Jackson ImmunoResearch Inc., West Grove, PA) and anti-guinea pig CF405M (Biotium ...
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bioRxiv - Cell Biology 2020Quote: ... Blotted proteins were incubated with corresponding primary antibodies at 4°C overnight followed by incubation with horseradish peroxidase conjugated secondary antibodies (Jackson ImmunoResearch, West Grove, PA, USA). Amersham ECL Western blotting detection reagent (GE Healthcare Life Sciences ...
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bioRxiv - Cancer Biology 2023Quote: ... for 10 minutes and washed 3 times in PBS for 5 minutes at room temperature (RT), followed by incubation for 30 minutes in blocking buffer (1% IgG-free BSA, 4% donkey serum (Jackson ImmunoResearch, West Grove, PA, USA) in PBS) ...
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bioRxiv - Neuroscience 2022Quote: ... Cat#ACC-102) over night at 4°C followed by 1 hour probing with donkey anti-rabbit HRP (1:10,000; Jackson ImmunoResearch Labs Cat#711-035-152). The membrane were imaged via enhanced chemiluminescence photodetection with the BioRad ChemiDoc XRS+ system.
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bioRxiv - Neuroscience 2024Quote: ... Membranes were incubated overnight at 4°C with the primary antibodies and then with the corresponding horseradish peroxidase-conjugated secondary antibodies (Jackson Immunoresearch, West Grove, PA, USA). All membranes were revealed using ECL chemiluminescence kit according to the manufacturer’s instructions (GE Healthcare ...
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bioRxiv - Neuroscience 2024Quote: ... and donkey anti-guinea pig Alexa Fluor (AF) 488 (Catalog #:706-545-148) had a 1:500 dilution and were stored overnight at 4 °C (Jackson ImmunoResearch; West Grove, PA, USA). Sections were then mounted and cover-slipped with using 50% glycerol in 0.02M KPBS and clear nail polish was used to seal the coverslip onto the slide.
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bioRxiv - Immunology 2020Quote: ... circumscribed with ImmEdge pen (Vector) and blocked in TBST (100mL Tris-HCl, 150mM NaCl, 0.05% Tween-20) containing 5% Donkey serum (Jackson ImmunoResearch #017-000-121) for 2 hours at RT ...
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bioRxiv - Genetics 2021Quote: ... The fixed tissues were then subjected to three rounds of 15 minute-each wash with 0.1% PBST (0.1% Triton X-100 in PBS) and blocked with 5% donkey serum (Jackson ImmunoResearch Laboratories, 107-000-121) for 1 hour at room temperature followed by incubation with primary antibodies in 5% donkey serum at 4°C ...
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bioRxiv - Immunology 2022Quote: ... cells were incubated for 1h at RT with blocking solution of 5% goat serum in PBS (Jackson ImmunoResearch, catalog no. 005–000-121). A rabbit polyclonal anti-SARS-CoV-2 nucleocapsid antibody (GeneTex ...
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bioRxiv - Plant Biology 2022Quote: ... the seedlings were washed for three times 5 min each and then incubated in the secondary antibody Cy-3-goat anti-mouse IgG (1:200; Jackson Immunoresearch, http://www.jacksonimmuno.com). The imaging was performed on a Nikon laser scanning microscope (Eclipse Ti equipped with Nikon C2 Si laser scanning unit ...
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bioRxiv - Neuroscience 2021Quote: ... The membranes were blocked in 5% w/v bovine serum albumin (IgG-free and protease-free BSA, Jackson ImmunoResearch Laboratories, West Grove, PA) in 50 mM Tris-HCl (pH 7.4) ...
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bioRxiv - Cancer Biology 2022Quote: ... Sections were washed 3 times for 5 minutes each in PBS+0.3% TritonX-100 and incubated with appropriate fluorescent conjugated secondary antibody (Jackson ImmunoResearch, West Grove, PA) for 45 minutes at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... Antibodies for O1 and O4 were diluted in N2B27 base medium supplemented with 5% Donkey Serum (v/v) (Jackson ImmunoResearch, 017-000-121) and added to wells for 18 minutes at 37°C ...
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bioRxiv - Developmental Biology 2024Quote: ... Sections of 20 um thickness were blocked in PBS + 0.3% Triton X-100 and 5% normal donkey serum (Jackson ImmunoResearch, 017-000-121) for one hour ...
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bioRxiv - Cell Biology 2024Quote: ... 0.1 M glycine, 0.1% Triton-X, syringe filtered) for 10 min, then blocking buffer (1X PBS, 5% goat serum (Jackson ImmunoResearch; 005-000-121), 2% bovine serum albumin (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2021Quote: ... Brains were then washed three times for 20 min each in PBST and incubated for 3 days at 4°C in a secondary antibody solution consisting of goat anti-mouse Cy3 (Jackson ImmunoResearch, AB_2338680, 1:200 v/v) and goat anti-rabbit Alexa Fluor 488 (Invitrogen ...
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bioRxiv - Physiology 2023Quote: ... Cells were then washed and incubated with primary antibodies diluted in blocking solution overnight at 4 °C followed by incubation with an AlexaFluor 488-conjugated AffiniPure Donkey Anti-Rabbit IgG secondary antibody (Jackson ImmunoResearch, 711-545-152, 1:500) for 1h at RT ...
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bioRxiv - Neuroscience 2022Quote: ... Pre-prepared immunopanning dishes were setup as follows: a 10cm petri dish was incubated at 4°C overnight with 30ul anti-rat antibody (Jackson ImmunoResearch Laboratories, Inc, 112-005-167) in 10 ml 50 mM Tris-HCL (pH 9.5) ...
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bioRxiv - Cell Biology 2023Quote: ... cells were fixed with 4% paraformaldehyde and immunofluorescence was performed as described previously [54] using appropriate primary and secondary antibodies (Jackson ImmunoResearch Laboratories, West Grove, PA, USA), and coverslips were mounted using Prolong Gold Antifade Reagent with 4′,6-diamidino-2-phenylindole (DAPI ...
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Differential impact of a dyskeratosis congenita mutation in TPP1 on mouse hematopoiesis and germlinebioRxiv - Cell Biology 2021Quote: ... deparaffinized sections were boiled in 0.1 M Sodium Citrate for 10 minutes for antigen retrieval and sections were blocked in suppressor serum (5% goat serum (Jackson ImmunoResearch #005-000-1210), 95% blocking solution (3% BSA and 0.5% Tween in PBS) ...
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bioRxiv - Neuroscience 2020Quote: ... The slides were washed 3 x 5 min with PBS and the secondary antibodies – donkey-anti-mouse Cy3 (1:500, Jackson ImmunoResearch, 715-165-151) and goat-anti-rabbit Alexa Fluor 488 (1:500 ...
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bioRxiv - Cancer Biology 2022Quote: ... Coverslips were washed with PBS 1X three times for 5 min at RT and incubated with Donkey anti-mouse IgG Alexa Fluor 488 secondary antibody (1:200; Jackson ImmunoResearch, 715-095-150) and Rhodamine-conjugated Phalloidin (Sigma ...
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bioRxiv - Developmental Biology 2022Quote: ... permeabilized with 0.1% Triton X-100 in PBS (PBST) for 30 min and blocked with 5% normal donkey serum (Jackson ImmunoResearch Laboratories, #017-000-121) diluted in PBST (blocking solution ...
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bioRxiv - Immunology 2020Quote: ... plates were washed 5 times with 0.05% PBST and then incubated with horseradish peroxidase (HRP)-conjugated anti-mouse IgG (Jackson ImmunoResearch Laboratories 115-035-003), IgG1 (Jackson ImmunoResearch Laboratories 115-035-205) ...
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bioRxiv - Developmental Biology 2023Quote: ... After washing once in PBS-T (PBS with 0.2% Tween-20), cells were blocked with blocking buffer (PBS-T, 2% BSA and 5% goat serum (Jackson Immunoresearch/Dianova, 017-000-121) for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... were washed in 1X TBS three times and then blocked and permeabilized for one hour in 1X TBS containing 5% Normal donkey serum (NDS, Jackson ImmunoResearch Laboratories, Lot #161590) and 0.5% Triton x-100 ...
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bioRxiv - Molecular Biology 2023Quote: ... After washing once in PBS-T (PBS with 0.2% Tween-20), cells were blocked with blocking buffer (PBS-T, 2% BSA, and 5% goat serum (Jackson Immunoresearch/Dianova, 017-000-121) for 1 hour at room temperature ...
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bioRxiv - Developmental Biology 2024Quote: P5-6 retinas were incubated in wash buffer (PBS with 0.1%Triton X100) with gentle rocking for 15 min and then in blocking buffer (wash buffer with 5% normal donkey serum (Jackson ImmunoResearch, 017-000-121)) for 1-2 hrs ...
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bioRxiv - Immunology 2024Quote: ... or cultured cells stained for the expression of surface markers with specific antibodies in PBS with 5% FBS and 20% normal goat serum (Jackson ImmunoResearch 005-000-121) and 0.01% NaN3 for 20 min on ice ...
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bioRxiv - Developmental Biology 2023Quote: ... Secondary antibody incubation was performed in 5% milk for 1 hour at room temperature using 1:5000 for anti-rabbit-HRP (Jackson Immunoresearch, 711-035-152) or anti-mouse-HRP (Jackson Immunoresearch ...
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bioRxiv - Neuroscience 2023Quote: ... the tissue was washed six times in PBS (5 min each) then incubated with a biotinylated goat anti-rabbit antibody (1:500; Jackson ImmunoResearch Laboratories; RRID: AB_2337965) prepared in NDS for 1 hr (RT) ...
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bioRxiv - Microbiology 2022Quote: ... The grids were washed on three drops of PBS before being incubated for 12 min with a 5 nm gold-labelled anti-rabbit secondary antibody (Jackson ImmunoResearch West Grove, PA) at dilutions of 1:10 ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were blocked for 1 hour with block buffer (0.05% IGEPAL CA-630, 0.05% Triton X-100, 5% goat normal serum [Jackson ImmunoResearch; 005-000-121] in 1xPBS). Samples were incubated with primaries/nanobodies diluted in block buffer for 16 hours at 4 °C on a rocker ...
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bioRxiv - Developmental Biology 2023Quote: ... The cells were then washed with PBS three times (5 minutes each) and incubated with Alexa Fluor 488 anti-mouse secondary antibody (1:1000, Jackson Immunoresearch #115-545-146) at 4°C overnight ...
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bioRxiv - Developmental Biology 2024Quote: ... Cells were then incubated at 4°C overnight with primary antibody at 1:100 in blocking buffer The following day cells were washed 3 times for 5 minutes with 1X TBS then incubated with Cy5 Donkey Anti-Mouse (Jackson ImmunoResearch 715-175-150) at 1:100 in 1X TBS for 2 hours in the dark at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... sections were washed in PBS (3×5 min) and incubated overnight with secondary antibody (peroxidase conjugated donkey-anti-rabbit 1:500, Jackson Immunoresearch, Baltimore, PA, USA). The Tyramide Signal Amplification procedure was then employed ...
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bioRxiv - Bioengineering 2024Quote: ... The cells were washed with 1X DPBS and then incubated in blocking buffer (consisting of 5% (v/v) normal donkey serum (Jackson ImmunoResearch, West Grove, PA) and 1% (w/v ...
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bioRxiv - Cell Biology 2024Quote: ... The following secondary antibodies were used at 1:10,000 dilution and incubated for 1 hour at room temperature in 5% milk w/ TBST: HRP donkey anti-rabbit (Jackson ImmunoResearch #711-035-152) and HRP goat anti-mouse IgG subclass 1-specific (Jackson ImmunoResearch #115-035-205) ...
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bioRxiv - Developmental Biology 2021Quote: ... Embryos were washed in PBST four times for 4 hours and incubated overnight with biotin goat anti-mouse IgG (Jackson ImmunoResearch ref. 115-066-071; 1:500) and then with streptavidin-Cy3 (Jackson ImmunoResearch ref ...
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bioRxiv - Bioengineering 2024Quote: ... Samples were washed five times with PTW and subsequently incubated with the respective secondary antibodies (1:500) for 24 h at 4°C (donkey anti-chicken Alexa Fluor 488 (Jackson ImmunoResearch Europe Ltd., Cat#: 703-545-155)) ...
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bioRxiv - Neuroscience 2022Quote: ... The sections were first washed in PBS three times for 5 min each and then incubated in blocking solution containing 10% normal donkey serum (017-000-121, Jackson ImmunoResearch, West Grove, PA, USA), 1% bovine serum albumen (A2153 ...
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bioRxiv - Microbiology 2020Quote: ... cells were incubated for 1 hour at room temperature with blocking solution of 5% goat serum in PBS (Jackson ImmunoResearch: catalog no. 005–000-121). To stain for SARS-CoV-2 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... treated with glycine (100 nM in PBS) and blocked with 5 % skim milk powder or 10 % normal goat serum (Jackson ImmunoResearch, West Grove, Pennsylvania, USA) and 1 % BSA in PBST for 1 h at 37 °C ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then washed (3 x 5 min in PBT) and incubated with fluorophore- conjugated streptavidin (Streptavidin AlexaFluor647, Jackson ImmunoResearch, 016-600-084, 1:1000) in 10% NDS in PBT for 2 hours at RT ...
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bioRxiv - Neuroscience 2023Quote: ... sections were washed 4 x 5 min in 0.1 M PB then incubated with a biotinylated secondary antibody (biotinylated donkey anti-mouse, Jackson ImmunoResearch, 715-065-151, 1:500) for 1 hour at RT ...
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bioRxiv - Neuroscience 2022Quote: ... RT) in PBS-T containing 5% NGS at the following concentrations: donkey anti guinea pig DyLight 405 (1:500, Jackson ImmunoResearch, West Grove, PA, USA), goat anti mouse Cy3 (1:500 ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were washed 3×5 min with PBS and stained with 1:1,000 donkey anti-chicken Alexa Fluor 488 secondary antibody (Jackson ImmunoResearch #703-545-155, RRID: AB_2340375), rinsed with PBS ...
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bioRxiv - Neuroscience 2023Quote: ... Cochleas were permeabilized in 0.2% Triton X-100 diluted in 1x PBS for 10 min and blocked in 5% normal donkey serum (Cat# 017-000-121; Jackson ImmunoResearch, West Grove, PA, USA) for 1 hour at room temperature ...