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Citations for Jackson ImmunoResearch :
451 - 500 of 569 citations for 6 Hydroxymethyl 4 methoxy 5 methyl nicotinic acid methyl ester since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... cells were carefully washed 3×5 min with PBS and incubated with secondary antibodies (Cat.# 711-545-152, 711-605-152, Jackson ImmunoResearch) for 1 h at RT ...
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bioRxiv - Neuroscience 2023Quote: ... Peroxidase-conjuguated AffiniPure F(ab’)2 Fragment Donkey Anti-Rabbit IgG (H+L) (1/5 000; Jackson ImmunoResearch; 711-036-152) for anti-Vinculin ...
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bioRxiv - Biochemistry 2023Quote: ... The nuclei were then quickly washed with assay buffer and resuspended in antibody incubation buffer (assay buffer with 5% NGS (Jackson ImmunoResearch), 0.02% digitonin ...
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Comparative membrane proteomics reveals diverse cell regulators concentrated at the nuclear envelopebioRxiv - Cell Biology 2023Quote: ... Samples were rinsed three times with PBS and blocked for 15 min using PBS with 5% goat serum (Jackson ImmunoResearch Laboratories) and 0.5% Triton X-100 (Tx ...
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bioRxiv - Neuroscience 2023Quote: ... slices were washed repetitively in TBS and TBS + 0.3% Triton X-100 and incubated with Cy2-conjugated streptavidin (5 µg/ml, Jackson Immunoresearch Lab.) for 2.5 hours at RT ...
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bioRxiv - Neuroscience 2022Quote: ... in 0.3% PBT were followed by 30 min incubation in blocking solution with 5% NGS (005-000-121, Jackson Immunoresearch Laboratories) in PBT ...
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bioRxiv - Neuroscience 2023Quote: ... Free-floating sections were washed three times for 10 minutes in 0.1M Tris-buffered saline (TBS) and then incubated in 0.1M TBS with 5% blocking buffer (Normal Donkey Serum, Jackson Immunoresearch, NC9624464) and 0.3% Triton-X 100 for 2 hours at room temperature ...
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bioRxiv - Immunology 2023Quote: ... sections were incubated with 0.3% Triton-X in PBS (wash buffer) for 30 minutes for permeabilization and then with 5% Normal goat serum (Jackson ImmunoResearch) in wash buffer (blocking buffer) ...
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bioRxiv - Developmental Biology 2023Quote: ... cells were washed in PBS/0.05% (three times, 5 min each) and incubated with Alexa Fluor (488, 568 and/or 647)-conjugated secondary antibodies (Jackson ImmunoResearch) diluted in blocking solution (1:200) ...
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were blocked in 5% skim milk and incubated with respective primary antibodies followed by HRP-conjugated secondary antibodies (Jackson ImmunoResearch Labs Cat# 111-035-045 ...
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bioRxiv - Biochemistry 2023Quote: ... Triton X-100 in PBS at room temperature for 15 min and washed with the blocking buffer [5% (v/v) normal goat serum (NGS, Jackson ImmunoResearch) and 0.1% (w/v ...
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bioRxiv - Cell Biology 2022Quote: ... Cochleas were permeabilized in 0.2% Triton X-100 in 1x PBS for 10 min and blocked in 5% normal donkey serum (Cat# 017-000-121, Jackson ImmunoResearch) diluted in 1x PBS (blocking buffer ...
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bioRxiv - Immunology 2023Quote: ... the sections were incubated in Cy-5 anti-rabbit secondary antibody (1:1000, Jackson ImmunoResearch Labs Inc., AB_2340607, West Grove, USA) for 1 hour at 4°C and counterstained with 4’,6-Diamidino-2-Phenylindole ...
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bioRxiv - Neuroscience 2023Quote: ... tissues were rinsed in PBS and blocked in Tris-Buffered Saline (TBS) supplemented with 5 % (vol/vol) Normal Donkey Serum (Jackson Immunoresearch) and 0.3 % (wg/vol ...
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bioRxiv - Biophysics 2023Quote: ... Blots were washed three times with TBST for 5 min prior to the addition of the secondary antibody (goat anti-rabbit tagged with horseradish peroxidase, Jackson ImmunoResearch, West Grove ...
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bioRxiv - Cell Biology 2023Quote: ... Detection was achieved using secondary antibodies conjugated to horseradish peroxidase (#111-036-003, 106-006-003, 1:5 mL, Jackson ImmunoResearch) and treated with enhanced chemiluminescence (#1705061 ...
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bioRxiv - Immunology 2023Quote: ... Purified human naïve B cells were cultured in 96-well plates in the presence of AffiniPure F(ab’)₂ Fragment goat anti-human IgG + IgM (5 μg/mL, CAT: 109-006-127, Jackson ImmunoResearch), anti-human CD40 (5 μg/mL ...
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bioRxiv - Neuroscience 2024Quote: ... the slices were rinsed 3 times for 5 minutes in PBS and incubated in the relevant secondary antibodies mix (donkey anti-sheep Alexa Fluor 405, Jackson Immunoresearch #713-475-003 ...
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bioRxiv - Immunology 2024Quote: ... diluted in 5% (wt/vol) dried milk in TBS-T and then with Peroxidase-AffiniPure Goat Anti-Rabbit IgG (Jackson ImmunoResearch) at 1 in 5,000 ...
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bioRxiv - Microbiology 2024Quote: ... except that primary antibodies were serially diluted 5-fold and followed by incubation with a 1:3000 dilution of HRP-labeled goat anti-human IgG (Jackson Immunoresearch) in casein for 45 min at 37°C ...
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Foxn3 is part of a transcriptional network that regulates cilia genes in the developing mouse retinabioRxiv - Developmental Biology 2024Quote: Retina sections were treated with a blocking solution (1% unlabeled adinity-purified Fab fragment donkey anti-mouse IgG (H+L) and 5% donkey serum (Jackson Immunoresearch) in PBS containing 0.1% Triton X-100 (ThermoFisher) ...
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bioRxiv - Developmental Biology 2020Quote: ... the embryos were incubated overnight at 4 °C in Cy3-conjugated goat anti-rabbit antibody (1:500 in BB; Jackson Immunoresearch, 111-165-144), Alexa488-conjugated goat anti-mouse (1:500 in BB ...
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bioRxiv - Systems Biology 2021Quote: ... and blocked with 1% BSA in carbonate buffer before being incubated overnight at 4°C with rabbit anti-sheep IgGs in carbonate buffer (Jackson ImmunoResearch, #313-005-003). 15 µL of cell lysate was incubated for 3 h at 4°C in the IgG-coated 384-well plates ...
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bioRxiv - Neuroscience 2020Quote: ... and the following secondary antibodies at 4°C for 24 h: Alexa Fluor 488 donkey anti-chicken (1:250, Jackson ImmunoResearch, #703-545-155) and Alexa Fluor 568 donkey anti-rabbit (1:250 ...
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bioRxiv - Biochemistry 2021Quote: ... cells were treated with 1 μM biotinylated peptides for 1h before they were fixed with 4% PFA and incubated with Streptavidin-AlexaFluor 594 antibody (Jackson ImmunoResearch, West Grove, PA) and Hoechst 33342 (Novus Biologicals ...
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bioRxiv - Developmental Biology 2022Quote: ... Larval brains were washed twice with 0.5% PBS-T and incubated for 1-7 days at 4°C in a blocking solution of 1% goat serum (Jackson ImmunoResearch, West Grove, PA), 1% donkey serum (Jackson ImmunoResearch ...
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bioRxiv - Bioengineering 2022Quote: ... Staining with primary antibodies was performed for 24 h (at 4 °C) with subsequently 2 h incubation with secondary antibodies (Jackson ImmunoResearch, West Grove, PA) diluted with 1 % BSA ...
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bioRxiv - Cell Biology 2023Quote: ... overnight at 4°C and then with 0.1 μg/ml Alexa Fluor 594-conjugated donkey polyclonal anti-rabbit IgG antibody (Jackson ImmunoResearch, West Grove, PA, USA) for 1 h at room temperature [6-8,10] ...
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bioRxiv - Genomics 2023Quote: ... for 1 hour, followed by incubation with primary antibodies (overnight, 4°C) and secondary antibodies (1-2 hours, from Jackson ImmunoResearch or ThermoFisher Scientific). The slides were then mounted with coverslips using VECTASHIELD (Vector Labs ...
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bioRxiv - Systems Biology 2023Quote: ... and blocked with 1% BSA in carbonate buffer before being incubated overnight at 4°C with rabbit anti-sheep IgGs in carbonate buffer (Jackson ImmunoResearch, #313-005-003). 15 µL of cell lysate was incubated for 3 h at 4°C in the IgG-coated 384-well plates ...
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bioRxiv - Neuroscience 2023Quote: ... Immunostaining was performed by incubating sections in blocking solution (PBS, 10% Donkey Serum, 1% Triton-100, 1h) followed by primary (overnight, 4°C) and secondary antibodies (1-2h, Jackson ImmunoResearch or ThermoFisher Scientific) incubation ...
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated overnight at 4°C in primary antibody solution containing 2% Normal Donkey Serum (NDS; Jackson Immunoresearch Laboratories Inc. 017-000-121) + 1xPBS + .03% Triton + primary antibodies (chicken anti-Iba1 ...
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bioRxiv - Neuroscience 2023Quote: ... Slides were then washed 4 × 30s in a tris buffer solution and the secondary biotinylated mouse adsorbed donkey anti-rabbit antibody (Jackson ImmunoResearch, 711-065-152) applied to all sections and incubated for 30 min ...
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bioRxiv - Neuroscience 2023Quote: ... slides were washed 3X 15 min in 0.1M PBS and then incubated at 4°C for 24 h with donkey anti-chicken-488 (1:1000; 703-545-155, Jackson Immunoresearch, West Grove, PA) and donkey anti-rabbit-CY3 (1:400 ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated overnight at 4°C in primary antibody solution containing 2% Normal Donkey Serum (NDS; Jackson Immunoresearch Laboratories Inc. 017-000-121) + 1xPBS + 0.03% Triton + primary antibody (rabbit anti-P2RY12 ...
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bioRxiv - Cell Biology 2020Quote: ... Blots were washed 3 x 5 mins in 1X TBST and incubated in 5% non-fat dry milk in 1X TBST containing anti-rabbit (H+L) horseradish peroxidase (Jackson ImmunoResearch Laboratories) secondary antibodies at a dilution of 1:20,000 for 1 hour at room temp ...
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bioRxiv - Cell Biology 2020Quote: ... Fc receptors were blocked by incubating cells in PBS supplemented with 5% FCS with 250 µ g.ml−1 purified mouse IgG (Jackson ImmunoResearch Laboratories). Antibody stainings were performed in PBS/5% FCS on ice for 30 minutes with optimal dilutions of commercially-prepared antibodies ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were washed 3 times for 5 minutes each with PBT (0.5% Triton X-100 in PBS) and incubated with the corresponding secondary antibodies Alexa Fluor 488 (Anti-Chicken IgG, Jackson ImmunoResearch Labs) and Cy3 (Anti-Rabbit IgG ...
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bioRxiv - Immunology 2020Quote: ... CAR detection was performed using anti-Fab specific antibody at day 5 post-transduction (109-606-006, Jackson ImmunoResearch, West Grove, PA).
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bioRxiv - Immunology 2021Quote: ... Plated peptides were incubated with the individual monoclonal antibodies (5 µg/ml diluted in blocking buffer) and further incubated with donkey anti-human alkaline phosphatase-conjugated secondary antibody (Jackson ImmunoResearch, USA). Immune complexes were identified following incubation with SIGMAFAST™ PNPP (SIGMA ...
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bioRxiv - Cell Biology 2021Quote: ... membrane was incubated with secondary Goat anti-Chicken HRP (1:5000 in 5%BSA/1X PBS-T, Jackson ImmunoResearch, 103-035-155) then incubated for 2 min in the dark with ECL Western Blotting Substrate (ThermoFisher ...
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bioRxiv - Cell Biology 2021Quote: ... it was incubated for 30 min in PBS with 5% non-fat milk and then for 1h at RT with horseradish peroxidase-conjugated secondary anti-mouse antibody (1:10000 dilution in PBS with 5% non-fat milk, Jackson Immunoresearch Laboratories), and washed again ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were subsequently washed four times for 5 minutes in TBS-T before secondary antibody (Jackson ImmunoResearch, 711-035-152, 1:15,000) was added for 1 hour at room temperature in 5% skim milk ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were permeabilized and blocked for 1–2 h at room temperature (RT) in 1X PBS with 0.5% Triton-X (0.5% PBST) and 5–10% normal donkey serum (NDS, Jackson ImmunoResearch 017-000-121). Primary and secondary antibodies were diluted in 0.5% PBST and 5% NDS ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were washed 3X using TBST-High Salt for 5 minutes on a rocking surface at room temperature before being incubated with secondary antibodies conjugated with Horseradish Peroxidase (Jackson ImmunoResearch Labs) for 45 minutes in 5% Non-fat dry milk rocking at room temperature ...
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bioRxiv - Immunology 2023Quote: ... before cultured in complete RPMI-1640 media without/with 5 μg/mL goat anti-mouse IgM (Jackson ImmunoResearch; cat # 115-006-075) and/or recombinant murine 25 ng/ml IL-4 (Peprotech ...
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bioRxiv - Developmental Biology 2023Quote: ... Samples were permeabilized and blocked in a single step with 0.2% Triton-X and 5% donkey serum (Jackson Immunoresearch, 017-000-121). Primary antibodies were subsequently added and incubated overnight at 4 °C ...
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bioRxiv - Microbiology 2023Quote: ... Slide regions were washed in PBS for 5 mins and then blocked with 10% normal goat (Jackson ImmunoResearch, West Grove, PA, USA) or donkey serum (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2023Quote: ... washed in PBST 3 times (5 min in each time) and incubated with secondary antibodies (mouse anti-Cy3, Jackson Immunoresearch,1:300) for 1h ...
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bioRxiv - Developmental Biology 2024Quote: Samples were washed twice for 10 minutes with PBS-T (0.5% Triton-X100 in PBS) and incubated with Cy3 and/or Alexa Fluor 647-conjugated secondary antibody (1:500, Jackson ImmunoResearch Laboratories), DAPI (4’,6-Diamidino-2-Phenylindole ...