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Citations for Beckman :
101 - 150 of 161 citations for Zika Virus VLP E prM M Proteins since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... subtilis spores were collected from the culture by the centrifugation at 8000 rpm at 4 °C for 20 min (Avanti J-E, Beckman Coulter Life Science, IN, USA). Supernatant was removed and the spore pellets were resuspended in fresh 100 mM PBS pH 7.4 ...
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bioRxiv - Biochemistry 2024Quote: ... Pellets were washed and gently resuspended in 1 mL of PBS and centrifuged at 50,000 x g for 1 h at 4°C (Beckman Optima MAX-E, TLA-55 rotor). Pellets were resuspended in 500 μl of PBS to obtain concentrated lentiviral stocks that were stored at −80°C in aliquots ...
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bioRxiv - Plant Biology 2021Quote: ... the samples were centrifuged at 35 000 RPM for 18 hours at 4°C in a 70Ti rotor and L8-M ultracentrifuge (Beckman Coulter). The ribosome pellets were then gently washed with RNase-free water and resuspended in 300 μl of resuspension buffer (100 mM Tris·HCl (pH 8.0) ...
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bioRxiv - Neuroscience 2020Quote: ... Each pool of samples was then purified using 79 μL beads buffer (20% PEG-8000 in 2.5 M NaCL, final concentrations) and 50 μL Ampure XP Beads (Beckman Coulter, A63881), and eluted in 7 μL NF H2O.
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bioRxiv - Systems Biology 2019Quote: ... were loaded on top of an 8 ml sucrose cushion (1.75 M sucrose in PEB) and centrifuged (18h, 90,000 g) using a Beckman Ti70 rotor (Beckman Coulter, Brea, USA). The resulting pellet was resuspended in 0.5 ml suspension buffer (200 mM Tris ...
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bioRxiv - Neuroscience 2023Quote: ... Supernatant were loaded on top of a discontinuous 0.75/1.0/2.33 M sucrose density gradient and centrifuge at 242,000 x g for 60 min at 4°C (Beckman SWTi41 swing rotor). The growth cone depleted fraction was collected between 0.75 M and 1.0 M sucrose ...
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bioRxiv - Cell Biology 2023Quote: ... ice-cold 5 mL 2.5 M sucrose was added to the bottom of an open-top plastic tube (Beckman-Coulter 344058), carefully topped up with ice-cold 5 mL 1.875 M sucrose ...
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bioRxiv - Plant Biology 2021Quote: ... cesium chloride continuous density gradient in resuspension buffer and centrifuged at 34,000 m for 5 hours at 20 °C using an SW41 Ti rotor (Beckman Coulter, Brea, CA). The virus particles were observed in the lower of two pale white bands in the gradient ...
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bioRxiv - Biochemistry 2019Quote: ... pH 7.4 containing 0.25 M sucrose and centrifuged at 53,000 rpm (150,000 g) in an Optima TLX ultracentrifuge (Beckman Coulter Inc., Brea, CA, USA) with a TLA100.3 rotor for 90 min at 4 °C ...
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bioRxiv - Biochemistry 2020Quote: ... pH 7.4 containing 0.25 M sucrose and centrifuged at 53,000 rpm (150,000 g) in an Optima TLX ultracentrifuge (Beckman Coulter Inc., Brea, CA, USA) with a TLA100.3 rotor for 90 min at 4 °C ...
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bioRxiv - Plant Biology 2020Quote: ... Soil pH and EC were 8.3 and 12 dS m−1 that were measured by a pH meter (Beckman 45 Modal, US) and EC meter (VWR Conductivity Meter DIG2052 ...
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bioRxiv - Immunology 2021Quote: ... was loaded onto the top of 6–8 M GuHCl gradients and centrifuged in a Beckman Optima L-90K Ultracentrifuge (Beckman SW40 rotor) at 40,000 rpm for 2.75 h 15°C ...
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bioRxiv - Bioengineering 2022Quote: ... The supernatant was transferred into polycarbonate tubes and centrifuged at 20,000 ×g for 45 min at 4°C to pellet down m/lEVs using an Optima XE-90 ultracentrifuge equipped with a 50.2 Ti rotor (Beckman Coulter, Indianapolis, IN). Next ...
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bioRxiv - Neuroscience 2022Quote: ... was loaded onto a sucrose gradient made up of 5 ml 1.2 M sucrose on the bottom and 5 ml of 0.8 M sucrose at the top in thin wall ultracentrifugation tubes (Beckman Coulter, Cat # 344059). The loaded gradient was ultracentrifuged at 69,150 x g for 1 h at 4 °C (SW 41 Ti Swinging-Bucket Rotor ...
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bioRxiv - Cell Biology 2023Quote: ... Supernatants were loaded onto a 1 M sucrose cushion and then pelleted by ultracentrifugation by using a TLA100.3 rotor (Beckman Coulter, cat#349490) for 1 hour at 100,000 rpm at 4°C ...
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bioRxiv - Synthetic Biology 2024Quote: ... 200 μL of supernatant was loaded onto sucrose gradients (10–50% sucrose in 0.6 M KP buffer) prepared in tubes (14 × 89 mm, Open-Top Thinwall Ultraclear tubes [Beckman Coulter, CA, USA]) using a Gradient Master (Beckman Coulter) ...
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bioRxiv - Bioengineering 2023Quote: ... The supernatant was then transferred into polycarbonate tubes and centrifuged at 20,000 ×g for 45 min at 4°C to pellet down m/lEVs using an Optima XE-90 ultracentrifuge fitted with a 50.2 Ti rotor (Beckman Coulter, Indianapolis, IN). Following that ...
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bioRxiv - Synthetic Biology 2019Quote: ... prior to CsCl density gradient ultracentrifugation (1.5 M CsCl) in Optima™ L90K centrifuge (rotor type: 70.1 Ti, tubes: 16 * 76 mm, Beckman Coulter, Brea, California, USA) for 65 h at 48,400 rpm and 4 °C ...
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bioRxiv - Microbiology 2021Quote: ... resuspended in 150 μl 0.1 M HEPES/NAOH pH 7.0 and purified for 48 h (250,000 × g, 4 °C; Beckman Optima LE-80K, rotor SW60-Ti) using a CsCl gradient (0.45 g/ml ...
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bioRxiv - Cell Biology 2023Quote: ... 2.5 M sucrose in HK was added to the reaction to a final concentration of 1 M (“Input” sample) and transferred to 7 × 20 mm polycarbonate centrifuge tubes (Beckman Coulter cat #343775). HK buffer with 0.75 M sucrose was gently laid on top of the reaction mixture followed by a layer of HK ...
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bioRxiv - Microbiology 2020Quote: ... The proteins were sedimented in the SW40Ti rotor (Beckman Coulter) at 100,000 × g for 17 h at 4 °C ...
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bioRxiv - Microbiology 2022Quote: Supernatant containing proteins and membrane were ultracentrifuged at 200,000xg (Beckman Coulter Ultracentrifuge ...
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bioRxiv - Biochemistry 2020Quote: ... proteins and liposomes were mixed in polycarbonate tubes (Beckman Coulter) and incubated for 30 min at RT ...
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bioRxiv - Physiology 2019Quote: ... Samples were then centrifuged at 100,000 xG at 4 °C for 20 hours in a swinging bucket rotor (Beckman L8-M Ultracentrifuge, SW28 Rotor).
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bioRxiv - Biochemistry 2024Quote: ... NaCl was then added to 1 M and the lysed solution was centrifuged at 20,000 g at 1°C for 45 minutes (Beckman Coulter J2-21, JA 20 rotor). Tween-20 was added to the supernatant to 0.1% (v/v ...
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bioRxiv - Neuroscience 2020Quote: ... The pH-insensitivity of the Ag/AgCl pellet was confirmed via measurements against a double junction Ag/AgCl reference electrode (Beckman Coulter, 3.5 M KCl, Item #A57189). We found the pH sensitivity of our custom-made EIROF deposited μRC electrodes to be 70 mV/log-decade at body temperature of 37°C (Fig ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein complexes were then fractionated by centrifugation in an SW 41Ti rotor (Beckman) at 38,000 rpm ...
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bioRxiv - Cell Biology 2019Quote: ... The protein solution was then centrifuged at 100,000 rpm in an Airfuge (Beckman Coulter) to remove aggregates ...
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bioRxiv - Cell Biology 2019Quote: ... Intracellular proteins were then stained with anti-Gag KC57 RD1 antibody (1/100, Beckman Coulter) and/or anti-α-tubulin (5 μg/mL ...
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bioRxiv - Neuroscience 2020Quote: ... aggregated proteins were removed by centrifugation at 30,000×g for 20 min (Beckman Coulter, USA). Protein was concentrated by centrifugal spin filters (Millipore ...
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bioRxiv - Bioengineering 2023Quote: ... green fluorescent protein (GFP)-positive cells were FACS-sorted (MoFlo Astrios, Beckman Coulter, California, USA) as single cells into a 96-well plate and clonally propagated ...
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bioRxiv - Genomics 2020Quote: The level of MPL protein on the platelet membrane was measured by flow cytometry (Beckman Coulter FC500) using the monoclonal antibodies ...
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bioRxiv - Biochemistry 2019Quote: ... the soluble Tau protein was centrifuged at 60,000 rpm for 1 hour (Optima Max XP Beckman Coulter) to remove aggregates present if any and 20 μM of soluble Tau protein was incubated in 20 mM BES buffer pH 7.4 with 5 μM of Heparin 17,500 Da in presence of 25 mM NaCl ...
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bioRxiv - Immunology 2024Quote: Antibodies and clones against the following proteins were used (clone, fluorophore, company): CD3 (UCTH1, PECy5, Beckman Coulter), CD7 (Horizon V450 or BUV395 ...
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bioRxiv - Microbiology 2023Quote: ... The surface expression level of S proteins (Figure 4B) was measured using CytoFLEX Flow Cytometer (Beckman Coulter) and the data were analyzed using FlowJo software v10.7.1 (BD Biosciences) ...
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bioRxiv - Biochemistry 2020Quote: ... The dialysed proteins were clarified by centrifugation in an Optima MAX-XP tabletop ultracentrifuge (Beckman Coulter Life Sciences) (90,720 x g ...
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bioRxiv - Cancer Biology 2022Quote: ... proteins enriched by IgG-agarose beads were eluted and processed with Agencourt AMPure-XP magnetic beads (Beckman Coulter) as previously described [40] ...
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bioRxiv - Cell Biology 2020Quote: ... Unbound protein was separated from liposomes by transferring 100μl of this mixture to 7×20mm PC ultracentrifuge tubes (Beckman) and carefully layering an equal volume of 0.75M sucrose buffer followed by 25μl of sucrose-free HK buffer ...
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bioRxiv - Cancer Biology 2020Quote: Cells expressing different fluorescent proteins were harvested from culture and counted using a ViCELL-XR (Beckman Coulter, Indianapolis, IN). Cells were washed 3 times in PBS to remove remaining cytokine ...
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bioRxiv - Cell Biology 2019Quote: ... 50 μg of each standard protein were loaded on top of the gradient and centrifuged at 29.000 rpm in a SW41 rotor (Beckman) for 29 h at 4°C ...
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bioRxiv - Cell Biology 2019Quote: ... The gradients were loaded with 50 μg of recombinant CLASP2 C-term or protein standards of known sedimentation coefficient and centrifuged for 29 h at 29.000 rpm in a SW41 rotor (Beckman). Fractions of 700 μl were collected starting from the bottom of the tube using a peristaltic pump ...
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bioRxiv - Systems Biology 2021Quote: ... Cells expressing the DsRed fluorescent protein were FAC sorted with a MoFlo cell sorter (Beckman Coulter GmbH, Krefeld, Germany) to obtain a highly enriched fraction for neuronal cells.
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bioRxiv - Microbiology 2023Quote: ... The denatured protein fraction was then removed via ultracentrifugation at 100,000 g for 20 min at 4°C (Beckman Coulter Optima XE-90 – IVD ultracentrifuge with a 42.2 Ti rotor) ...
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bioRxiv - Neuroscience 2022Quote: ... The amount of p24 capsid protein was determined by the HIV-1 p24 ELISA antigen assay (Beckman Coulter, Fullerton, CA). Virus from different productions averaged 175 ng/µl of p24 antigen.
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bioRxiv - Biochemistry 2020Quote: ... Protein expression was continued for 24 h before harvesting cells by centrifugation (4°C, 8000 rpm, 10 min in Beckman Coulter Avanti JXN-26 centrifuge ...
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bioRxiv - Biochemistry 2020Quote: ... Protein expression was continued for 18 h before harvesting cells by centrifugation (4°C, 8000 rpm, 10 min in Beckman Coulter Avanti JXN-26 centrifuge ...
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bioRxiv - Biochemistry 2021Quote: ... 300 μL of metabolite pool was aliquoted per target protein replicate and sealed with aluminum foil seals (Beckman Coulter 538619) to create the metabolite chambers ...
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bioRxiv - Biochemistry 2020Quote: ... Protein-liposome complexes were separated from unbound protein by centrifugation (2 h at 45.000 rpm, 4 °C, MLS50 rotor -Beckman Coulter) in the density gradient ...
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bioRxiv - Microbiology 2023Quote: ... Not solubilised protein was removed by a second pelleting at 100×kg for 1 h in Ti70 rotor (Beckman Coulter). The solubilized and denatured protein was loaded on a 5 ml HisTrap HP column (Cytiva) ...
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bioRxiv - Molecular Biology 2023Quote: ... the extract and the protein ladder were overlaid onto separate gradients and centrifuged with an SW41Ti rotor (Beckman Coulter, 20U113807) in an Optima XPN-100 Ultracentrifuge (Beckman Coulter ...