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Citations for Beckman :
1 - 50 of 161 citations for Zika Virus VLP E prM M Proteins since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: CCMV-SS and CCMV-Ctt830-SS VLPs were separated from cellular proteins by ultracentrifugation (SW28 rotor, Beckman, Palo Alto ...
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bioRxiv - Molecular Biology 2019Quote: ... the VLPs were pelleted for 2 h at 40,000 rpm (Beckman TLA55) at 4°C and subsequently resuspended in PBS (pH 7.4) ...
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bioRxiv - Biophysics 2020Quote: ... VLPs were pelleted for 2 h at 40,000 rpm (Beckman TLA55 rotor) at 4°C and solved in PBS (pH 7.4).
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bioRxiv - Molecular Biology 2019Quote: ... The VLPs in the supernatant were concentrated by ultracentrifugation at 35,000 rpm (Beckman Ti45) for 2 h at 4°C and then further purified using CsCl equilibrium gradient ultracentrifugation at 35,000 rpm (Beckman SW56 ...
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bioRxiv - Biophysics 2020Quote: ... VLPs in the supernatant were concentrated by ultracentrifugation at 35,000 rpm (Beckman Ti45 rotor) for 2 h at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... the VLPs were concentrated by ultracentrifugation (40,000 rpm, 2h, 10°C, Beckman SW41 rotor), resuspended in buffer A and observed by TEM ...
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bioRxiv - Biophysics 2020Quote: ... VLPs were further purified using CsCl equilibrium gradient ultracentrifugation at 35,000 rpm (Beckman SW56 rotor) for 18 h at 4°C ...
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bioRxiv - Immunology 2021Quote: ... VLPs were concentrated using ultracentrifuge (TLA100.3 rotor, Beckman; at 72,000 rpm for 1 hour, +5°C) or by ultrafiltration using Amicon Ultra 15 (100 kDa ...
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bioRxiv - Biophysics 2021Quote: ... 24h after transfection cells media (9mL) were filtered before performing VLPs purification by ultracentrifugation (SW41Ti rotor (Beckman) 29 000rpm ...
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bioRxiv - Microbiology 2021Quote: ... VLPs were concentrated by ultracentrifugation through a 20% (w/w) sucrose cushion using a Beckman SW32Ti rotor (Beckman-Coulter) at 20,200 rpm for 2h at 4°C.
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bioRxiv - Microbiology 2021Quote: ... The VLPs were concentrated by ultracentrifugation for 2 h at 31,000 rpm at 4 °C (Beckman SW-31Ti rotor), and then resuspended in 500 μL of Ex-Cell 405 medium ...
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bioRxiv - Microbiology 2022Quote: ... Pseudotypes and VLPs were then concentrated by ultracentrifugation (20,000 RPM, 4 ºC, 1.5h, Beckman Coulter Optima XPN-100, SW32Ti rotor) through a 20% (w/v ...
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bioRxiv - Microbiology 2020Quote: ... Whole blood staining was done with Alexa Fluor®647 labelled-VLP (2.5µg/ml) and cell subsets were discriminated using the following antibody panel: CD45 (Clone J33; Beckman Coulter, Brea, CA), CD11c (Clone BU15 ...
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bioRxiv - Immunology 2021Quote: ... ultra-centrifugation of virus culuture supernatant and the virus pellet in 30% sucrose cushion gradient were performed using Optima-L 100K ultracentrifuge (Beckman Coulter) at 18,000 RPM for 1.5 hours ...
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bioRxiv - Biochemistry 2022Quote: ... VLPs were concentrated over 500 μL of 20% sucrose in PBS at 50,000 x g for 45 min (Beckman Coulter Optima TLX). Infections were assessed by western blot using the following antibodies ...
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bioRxiv - Genetics 2023Quote: ... in-house VLPs were produced using the same protocol followed by ultracentrifugation at 24,000 RPM for 2 hours (SW 28 Beckman swinging bucket rotor) to concentrate the VLPs and avoid diluting the HSPCs culture media ...
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bioRxiv - Cell Biology 2020Quote: ... spinning at 3,200 rpm (Beckman J-6M/E centrifuge). The centrifuge chamber was set at 4°C during all centrifugation steps until approximately 10 min before cell harvesting ...
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bioRxiv - Microbiology 2024Quote: ... The virus was enriched by ultracentrifugation (Beckman Coulter Optima™ L-80XP) at 246,347g for 4 hours at 4 °C ...
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bioRxiv - Genomics 2021Quote: Beckman Coulter Avanti J-E centrifuge (Beckman Coulter, cat, no. A20698)
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bioRxiv - Microbiology 2020Quote: ... the virus particles were pelleted by ultracentrifugation in a Beckman type 19 rotor (Beckman Coulter ...
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bioRxiv - Synthetic Biology 2020Quote: Concentrated virus was prepared by ultracentrifugation (Beckman Coulter Optima XE-90, rotor SW 28) at 20,000 rpm through a 6% iodixanol gradient (Sigma Aldrich ...
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bioRxiv - Immunology 2022Quote: ... Virus was concentrated using 20% sucrose and ultracentrifugation at 24,000 rpm (Beckman SW28 rotor) for 2h at 4 °C ...
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bioRxiv - Molecular Biology 2020Quote: ... 1.3 M, 2.5 M) as indicated in the method (Vella et al., 2017) in ultra-clear SW40Ti tubes (Beckman Coulter). The sucrose gradients were centrifuged at 200,000 x g avg at 4°C for 173 min using a SW40Ti rotor (Beckman Coulter) ...
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bioRxiv - Neuroscience 2020Quote: ... A sucrose density gradient was prepared with 5 ml of both 0.8 M and 1.2 M sucrose in thin wall ultracentrifugation tubes (Beckman Coulter, Cat # 344059). P2 homogenate was mixed with 1 ml of homogenizing buffer ...
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bioRxiv - Cell Biology 2019Quote: ... and spun for 1 hour in a MAX-E tabletop ultracentrifuge (Beckman) at 4°C and 100,000 × g (47,000 rpm ...
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bioRxiv - Biochemistry 2023Quote: ... The lysed cells were pelleted via centrifugation (Beckman Coulter, Avanti J-E) at 40,000 × g for 35 min ...
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bioRxiv - Synthetic Biology 2019Quote: ... Concentrated virus was prepared by ultracentrifugation (Beckman Coulter Optima XE-90, rotor SW 32 Ti) at 20,000 rpm through a 6% iodixanol gradient (Sigma Aldrich ...
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bioRxiv - Physiology 2023Quote: ... Commercial radioimmunoassay kits (M/s Beckman Coulter IM 1188) with analytical sensitivity of 0.03 ng/mL and including a highly specific progesterone antibody were used for progesterone estimation ...
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bioRxiv - Microbiology 2023Quote: ... Virus was separated from free dye by ultracentrifugation as above on a SW60Ti rotor (Beckman Coulter). The virus band was extracted and desalted on a NAP column (VWR ...
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bioRxiv - Biochemistry 2022Quote: ... Packed eggs were crushed by centrifugation (BECKMAN, Avanti J-E, JS13.1 swinging rotor) for 20 min at 18,973×g ...
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bioRxiv - Molecular Biology 2020Quote: ... Packed eggs were crushed by centrifugation (BECKMAN, Avanti J-E, JS13.1 swinging rotor) for 20 min at 18973 × g ...
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bioRxiv - Biochemistry 2023Quote: ... The lysed cell contents were pelleted via centrifugation (Beckman Coulter, Avanti J-E) at 40,000 × g for 35 min ...
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bioRxiv - Biochemistry 2024Quote: Packed eggs were crushed by centrifugation (BECKMAN, Avanti J-E, JS13.1 swinging rotor) for 20 min at 18,973×g ...
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bioRxiv - Cancer Biology 2024Quote: ... 10,000 × g for 30 min) at 4°C (Beckman Coulter Avanti J-E Centrifuge); filtered through a 0.22 μm PES filter (Genesee) ...
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bioRxiv - Biochemistry 2021Quote: ... The virus-containing filtrate was then further purified using an ultracentrifuge (Optima XPN-90 Ultracentrifuge, Beckman Coulter, USA) with a cycle of 1,000 rpm (116 g ...
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bioRxiv - Biochemistry 2022Quote: ... The lysate was centrifuged at 18000 rpm for 30 minutes (Beckman Coulter Avanti J-E) and the supernatant was used for further purification ...
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bioRxiv - Molecular Biology 2022Quote: ... Eggs were packed into tubes by centrifugation (BECKMAN, Avanti J-E, JS-13.1 swinging rotor) for 1 min at 190 xg and crushed by centrifugation for 20 min at 18,973 xg ...
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bioRxiv - Microbiology 2020Quote: ... Samples were centrifuged at 6000 RPM for 10 mins (Avanti-J-E HPC, Beckman, USA), and the pellet was resuspended in an equal volume of isolation buffer (50 mM 3-(N-morpholino ...
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bioRxiv - Biochemistry 2022Quote: ... Eggs were packed into tubes by centrifugation (BECKMAN, Avanti J-E, JS-13.1 swinging rotor) for 1 min at 190×g and crushed by centrifugation for 20 min at 18,973×g ...
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bioRxiv - Cancer Biology 2024Quote: ... and spinning at 12,535 g for 8 s (Beckman E centrifuge, Beckman Coulter, Fullerton, CA). The tube was placed briefly in liquid nitrogen ...
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bioRxiv - Microbiology 2020Quote: ... The virus was then purified over a 20% sucrose cushion by centrifugation at 35,000 rpm (Beckman Coulter SW41 rotor) for 3 h at 4°C ...
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Human Sex Matters: Y-linked lysine demethylase 5D drives accelerated male osteogenic differentiationbioRxiv - Cell Biology 2021Quote: ... Virus was transferred into conical tubes and spin at 20000rpm for 2 hours at 4°C in (Beckman TST2838) swinging bucket rotor ...
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bioRxiv - Molecular Biology 2020Quote: ... Virus particles in the supernatant were pelleted by ultracentrifugation at 45.000 rpm for 45 min (Beckman 70.1 Ti Rotor) over a pad of 15% sucrose in 10 mM Tris-HCl buffer (pH 7.4) ...
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bioRxiv - Microbiology 2024Quote: ... Virus RNA was extracted with a magnetic bead-based RNA extraction kit (RNAdvance Viral Reagent kit, Beckman Coulter, C63510) on an epMotion® 5073t liquid handler (Eppendorf ...
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bioRxiv - Molecular Biology 2019Quote: ... 1 M sucrose) in a 11×34 mm polypropylene tube (Beckman Coulter) and centrifuged for 6 hours at 55,000 rpm at 4°C on a TLS-55 rotor (Beckman Coulter ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were then centrifuged in an Optima MAX-E tabletop ultracentrifuge (Beckman Coulter, Brea, CA, USA) at 184,000 x g (65,000 rpm ...
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bioRxiv - Cell Biology 2022Quote: ... the virus-cell mixture was centrifuged at 1,000 rcf for 2 hours at 32°C (Beckman Coulter, Allegra X-30R). Infected cells were then cultured as described above and subsequently FACS-sorted for the establishment of clonal cell lines.
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bioRxiv - Biophysics 2023Quote: ... Virus-containing supernatants were collected at 48 hours and 72 hours after transfection and concentrated using ultracentrifugation (Ultraclear, Beckman 344058). The transduction capacity of the viral vector product was confirmed by culturing non-transfected HEK293T cells with the virus after 24 hours and assessing via fluorescent imaging.
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bioRxiv - Microbiology 2020Quote: ... Bacterial cells were harvested by centrifugation at 5000 rpm (JLA 9.1000 rotor, Beckman Coulter Avanti J-E) for 20 min and re-suspended in 100 ml lysis buffer A (50 mM Tris pH 8.0 ...
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bioRxiv - Microbiology 2021Quote: ... The cells were collected by centrifugation at 4000 rpm in an Avanti J-E centrifuge (Beckman Coulter) with a JA-14 rotor at 4°C during 30 min and pellets were frozen at -80°C until further processing.