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Citations for Beckman :
251 - 300 of 394 citations for Transcription Factor 15 TCF15 Antibody FITC since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... cellular/protein debris were then collected by centrifugation at 1,000xg for 15 minutes at 4°C (Beckman Coulter Allegra X-14R), and the supernatant (DNA ...
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bioRxiv - Biochemistry 2023Quote: ... Mitochondrial crude was pelleted by centrifugation at 9500 ×g (4 °C, 15 min) using the Optima XE-90 Ultracentrifuge with a Ti-45 rotor (Beckman Coulter). The final pellet was resuspended in 2 mL of Resuspension buffer (250 mM sucrose ...
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bioRxiv - Biochemistry 2023Quote: ... then centrifuged at 27,000 gmax for 15 min at 4°C (Optima XPN-100 Ultracentrifuge, Beckman Coulter, with SW60 Ti rotor). Resuspension of the pellet (sub-mitochondrial particles ...
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bioRxiv - Cancer Biology 2024Quote: ... using a combined Annealing/Extension step at 63°C for 10 seconds and 15 cycles followed by a 1.1X post-amplification AMPure XP (Beckman Coulter, A63880) bead cleanup ...
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bioRxiv - Molecular Biology 2024Quote: ... and 1U of FastAP (TFS, EF0654) for 15 min at 37 °C and purified using 1.2× SPRIselect beads (Beckman Coulter, B23318). The inDrops-v1.1 libraries were digested with 40U of ExoI and 1U of FastAP for 15 min at 37 °C ...
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bioRxiv - Genomics 2024Quote: ... c) increasing PCR cycles (15 in total) and eliminating primer dimers prior to sequencing (Agencourt AMPure XP magnetic beads, Beckman Coulter). Primary PDAC were sequenced on the NovaSeq6000 ...
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bioRxiv - Biophysics 2021Quote: ... which were centrifuged at 3000 rpm for 15 minutes at 4°C using a fixed angle centrifuge (Beckman Coulter, Brea, CA, USA). After removal of excess supernatant ...
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bioRxiv - Cell Biology 2020Quote: ... The resulting supernatant containing AAV particles was added to the top of an iodixanol gradient (15%, 25%, 40%, 60% top to bottom) in an Optiseal tube (Beckman Coulter #361625). The gradient was then centrifuged using a Beckman Ti-70 rotor in a Beckman XL-90 ultracentrifuge at 67,000rpm for 70min ...
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bioRxiv - Cell Biology 2020Quote: ... tSETD2-Flag plasmid was transfected into HEK293 Freestyle cells with FectoPRO transfection reagent (116-010) and cells were harvested 36-48 hours later at 5,000 rpm for 15 mins (Beckman JLA 8.1, 363563). The pellet was suspended in lysis buffer (50 mM HEPES pH 7.5 ...
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bioRxiv - Neuroscience 2020Quote: ... Final 15 mL of solution was mixed by inverting the tube 10x and carefully pipetted into 2 ultracentrifuge tubes (Beckman Coulter #344059) onto the isolation buffer cushion (5 mL ...
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bioRxiv - Plant Biology 2021Quote: ... The resulting slurry was centrifuged for 15 min at 9000 rpm at 4 °C using a JA14 rotor (Beckman Coulter, Brea, CA). The supernatant was filtered with a Miracloth and kept on ice ...
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bioRxiv - Biochemistry 2020Quote: tSETD2-FLAG was transfected into HEK 293 Freestyle cells with FectoPRO transfection reagent (Polyplus, 10118-444) and cells were harvested 48 hours later at 5K rpm for 15 mins (Beckman JLA 8.1).The pellet was suspended in lysis buffer (50 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... Harvesting of P2 viral stocks was carried out by centrifuging cell suspensions in 50 mL Falcon tubes at 2281 RCF (Beckman GS-15), collecting the supernatant in a fresh sterile tube ...
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bioRxiv - Cancer Biology 2021Quote: ... transferred to 96-well plates and incubated 15 min at room temperature with Agencourt AMPure XP magnetic beads (Beckman Coulter Cat# A63881) at a 1:1 ratio ...
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bioRxiv - Biochemistry 2021Quote: ... Harvesting of P2 viral stocks was carried out by centrifuging cell suspensions in 50 mL Falcon tubes at 2281 RCF (Beckman GS-15), collecting the supernatant in a fresh sterile tube ...
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bioRxiv - Cell Biology 2022Quote: ... The plate was incubated for 15 min in darkness and measured in the flow cytometer FC 500 (Beckman-Coulter, Brea, CA, USA). This experiment was repeated 3 times on different days.
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bioRxiv - Neuroscience 2022Quote: ... Cell lysate was then loaded on an iodixanol gradient (60, 40, 25 and 15% iodixanol, ELITechGroup #1114542) in Beckman Quick-Seal Polyallomer tubes (Beckman-Coulter #342414) which were sealed and centrifuged in a Beckman-Coulter Optima XE-90 Ultracentrifuge at 16 °C and 69,000 rpm for 1 hour and 10 minutes ...
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bioRxiv - Biochemistry 2022Quote: ... Harvesting of P2 viral stocks was carried out by centrifuging cell suspensions in 50 mL Falcon tubes at 2281 RCF (Beckman GS-15). To the supernatant containing virus ...
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bioRxiv - Microbiology 2022Quote: ... The supernatant was collected and purified through 15-45% (w/v) sucrose density gradient by ultracentrifugation at 151,000 × g (using a 17 mL Beckman SW32.1 Ti rotor) for 3 hours at 10°C (18) ...
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bioRxiv - Microbiology 2022Quote: ... Supernatant was purified through 15-45% (w/v) sucrose density gradient by ultracentrifugation at 151,000 × g (using a Beckman SW 40 rotor) for 3 hours at 10°C (18).
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bioRxiv - Microbiology 2022Quote: ... Supernatant was purified through 15-45% (w/v) sucrose density gradient by ultracentrifugation at 151,000 × g (using a 17 mL Beckman SW32.1 Ti rotor) for 3 hours at 10°C (16) ...
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bioRxiv - Immunology 2023Quote: ... After adding 450 μL of RBC lysis solution (QuantoBio Technology, Beijing, China) and 15 min incubation the samples were analysed using a DxFLEX flow cytometer (Beckman Coulter, USA). All data were analysed using FlowJo X (FlowJo ...
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bioRxiv - Biochemistry 2023Quote: ... Harvesting of P2 viral stocks was carried out by centrifuging cell suspensions in 50 mL Falcon tubes at 2281 RCF (Beckman GS-15). To the supernatant containing virus ...
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bioRxiv - Microbiology 2023Quote: ... Cells were incubated further overnight at 20°C and subsequently harvested by centrifugation (Beckman rotor 8.1000, 5000 rpm, 15 min, 4°C). The pellet was resuspended in PBS ...
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bioRxiv - Immunology 2019Quote: Purified antibodies (BioLegend, BD, Beckman Coulter) were conjugated to stable metal isotopes using commercially available polymeric chelators (“X8,” Fluidigim ...
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bioRxiv - Cell Biology 2021Quote: ... and PAR1 antibodies (Beckman Coulter, #IM2584) and developed by chemiluminescence.
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bioRxiv - Immunology 2020Quote: ... recombinant protein and CD14 antibody (Beckman) were used at 20 μg/ml ...
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bioRxiv - Biophysics 2023Quote: ... 4b4 antibody was purchased from Beckman Coulter.
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bioRxiv - Immunology 2023Quote: ... VersaComp Antibody Capture Kit (Beckman Coulter) was used to set compensation to correct for spectral overlap ...
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bioRxiv - Microbiology 2020Quote: ... CLP assembly reactions were incubated at 25°C for 30 min before loading onto 15-30% (wt/wt) sucrose gradients and spinning at 159,599 X g for 43 min at 4°C (Beckman Coulter TLS 55 rotor). Gradients were immediately fractionated and aliquots of each fraction were denatured by boiling in 1% SDS to ensure antibody access ...
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bioRxiv - Neuroscience 2019Quote: ... The homogenates were then centrifuged for 15 minutes at 1500 × g (5000 rpm) in a rotor (Beckman Coulter Ti50, USA, CA, Palo Alto). The supernatant was analyzed using a Fish Glutamine Synthetase ELISA Kit (MBS ...
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bioRxiv - Bioengineering 2020Quote: ... and the supernatant was centrifuged again at 26000 × g at 4 °C for 15 min using an Optima L-90K (Beckman Coulter, Brea, CA) ultracentrifuge with a 70-Ti fixed angle rotor (Beckman Coulter) ...
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bioRxiv - Biophysics 2021Quote: ... Different ratios of LUVs and cyt c (with the protein concentration kept at 5 μM) were incubated in the sample buffer at room temperature for 15 min in ultracentrifugal tubes (Beckman Coulter, Indianapolis, IN). The effective PG/cyt c molar ratio (R ...
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bioRxiv - Bioengineering 2021Quote: ... The tubes were then centrifuged at 1000 g for 15 minutes at 4°C using a Microfuge™ 22R (Beckman Coulter, Brea, CA), and the serum was then stored at -80°C ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 mM ATP and 1 mM PMSF) and centrifuged for 15 h at 4°C and 74 400 x g (Beckman SW 32.1 Ti). From the gradient ...
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bioRxiv - Neuroscience 2019Quote: ... the cell lysates are loaded to the gradients of iodixanol (15%, 25%, 40% and 60%) in the OptiSeal tubes (Beckman Coulter, Indianapolis, IN). Then the lysates were spin down at 67,000 rpm for 1 hour at 18 °C with the ultracentrifuge (Beckman) ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were spun down at 4°C for 15 minutes at 4000 RPM (3040 RCF) in a J6 centrifuge (Beckman Coulter Life Sciences). Supernatant was discarded and pellets were stored in 15ml conical tubes in -80°C ...
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bioRxiv - Microbiology 2022Quote: ... 100 ml cell cultures were grown over night and harvested by centrifugation at 15,000 x g for 15 min at 4 °C (Beckman JLA-16.250 rotor, Beckman Avanti J-25, Beckman Coulter). Pellets were washed with 20 mM potassium phosphate (pH 7.5 ...
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bioRxiv - Microbiology 2023Quote: ... cells were pelleted by centrifugation at 6,000 ×g at 4 °C for 15 min with ALLEGRA X-30R (Beckman Coulter, Brea, CA, USA). The supernatant was centrifuged at 15,000 ×g at 4 °C for 15 min with Sorvall LYNX4000 (Thermo Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... Expression was continued for 16h and the cells harvested by centrifugation at ∼6250g for 15 min at 4°C (Beckman Coulter centrifuge, JLA8.1000). The RING Zn-finger domains from both RING1B and PCGF4 subunits were cloned into a modified polycistronic expression vector (pET29a ...
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bioRxiv - Biochemistry 2023Quote: ... Expression was continued for 7h and the cells harvested by centrifugation at ∼6250g for 15 min at 4°C (Beckman Coulter centrifuge, JLA8.1000).
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... the cell lysate was layered on top of a discontinuous OptiPrep® gradient (50 %, 35 %, 30 %, 20 %, and 15 %) and centrifuged at 135,000 g for 1 h at 4°C (Beckman L8-80M Ultracentrifuge, USA). The melanin pellet was recovered ...
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bioRxiv - Physiology 2023Quote: ... blood samples were centrifuged at 3000 RPM for 15 minutes at four °C in a refrigerated centrifuge (Beckman Coulter Allegra X-22 Series). Plasma samples were aliquoted and stored at -80°C ...
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bioRxiv - Immunology 2019Quote: ... The ligation reaction was incubated on the Agilent workstation at 20°C for 15 minutes and then purified and size selected with an AMPure XP workflow (Beckman Coulter, Cat No. A63880). 20 µl of KAPA HiFi HS Ready Mix (Kapa Biosystems ...
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bioRxiv - Microbiology 2023Quote: ... and 1.45 g/mL layers and centrifuged for 1 h at 28,500 rpm at 15 °C using an Optima XE-90 Ultracentrifuge and SW 41 Ti Swinging-Bucket Rotor (Beckman Coulter, Inc., Pasadena, CA, USA). After centrifugation ...
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bioRxiv - Genomics 2023Quote: ... The volume was adjusted to 50 ml by adding more distilled water and the suspension was centrifuged at 3750 rpm at room temperature for 15 min with a clinical centrifuge (Allegra X-12R, Beckman Coulter, Brea CA, USA). The pellets were resuspended in 1–3 ml TE buffer (10 mM Tris-HCL ...
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bioRxiv - Immunology 2021Quote: ... recombinant protein and anti-CD14 antibody (Beckman) were used at 20 μg/ml ...
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bioRxiv - Cell Biology 2023Quote: ... 4B4 integrin inhibiting antibody (Beckman Coulter, 41116015), vinculin (Sigma ...
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bioRxiv - Immunology 2023Quote: ... The following antibodies were purchased from Beckman Coulter ...
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bioRxiv - Immunology 2023Quote: ... anti-p24 KC- 57 antibody (Beckman Coulter), 2G12 anti-gp120 antibody (NIH HIV Reagent program ...