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Citations for Beckman :
101 - 150 of 394 citations for Transcription Factor 15 TCF15 Antibody FITC since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... the fluorescence of Bodipy-C11 was collected from the FITC channel on a Gallios cytometer (Beckman-Coulter).
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bioRxiv - Molecular Biology 2022Quote: ... Reverse transcription reactions were cleaned up using AMPure XP Reagent magnetic beads (Beckman Coulter). For EBs RT-qPCR ...
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bioRxiv - Cell Biology 2022Quote: ... The supernatant was centrifuged at 200,000 x g for 2.5 h (high g) at 4°C in a TLA-100.3 fixed angle rotor (k-factor 14) (Beckman Coulter) and the pellet was resuspended in 25 ∝l lysis buffer ...
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bioRxiv - Cell Biology 2022Quote: ... The supernatant was centrifuged at 200,000 x g for 2.5 h (high g) at 4°C in a TLA-100.3 fixed angle rotor (k-factor 14) (Beckman Coulter) and Red-C12 fluorescence of the starting material ...
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bioRxiv - Biochemistry 2022Quote: ... 4°C for 15 min using a JLA-9.1000 centrifuge rotor (Beckman). The pellet was resuspended in 70 mL buffer containing 50 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2020Quote: ... columns were spun at 100.000 g for 24 hours using a SW-28.1 rotor (k-factor: 276) in an optima XPN-80 ultracentrifuge (Beckman coulter), and subsequently fractions of 1 mL were collected ...
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bioRxiv - Cell Biology 2022Quote: ... The supernatant was centrifuged at 300,000×g at 4°C for 3 h in a TLA-110 fixed angle rotor (k-factor 13) (Beckman Coulter). The pellet was resuspended in 10 µL PBS of which 3 µL was adsorbed onto glow-discharged 2 nm carbon layer coated Quantifoil R1.2/1.3 400 mesh gold grids ...
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bioRxiv - Systems Biology 2023Quote: ... The urine was centrifuged at 20,000 x g for 30 minutes at 4°C (k-factor 1,790) in an Optima XPN-80 ultracentrifuge (Beckman Coulter) equipped with a SW32 Ti swinging bucket rotor (Rmin 67 ...
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bioRxiv - Immunology 2022Quote: ... Staining and bacterial count (events/μl in the FITC +ve gate) was assessed by flow cytometry (CytoFLEX, Beckman-Coulter). The pH responsiveness of CypHer5E was tested by measuring the bacterial fluorescent staining in the APC channel before and after the addition of 1 μl of sodium acetate (3 M ...
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bioRxiv - Microbiology 2021Quote: ... Gating in SSC‐A and GFP FITC‐A channels in the flow cytometry analysis software (Beckman Coulter Cytexpert 2.3.0.84) allows finding of (fluorescent ...
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bioRxiv - Immunology 2021Quote: ... using a 12-parameter panel (CD57-FITC, CD4-PE, CD161-PC7, CD8-KrO, CD279-PC5.5, CD45RA-A700, CD3-APC-A750 [Beckman Coulter] and CD56-BV605 ...
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bioRxiv - Immunology 2022Quote: ... human Vγ9Vδ2 T lymphocytes were collected and stained with a FITC-labeled anti-human TCR Vδ2 mAb (#IMMU389, Beckman Coulter) and analyzed by flow cytometry ...
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bioRxiv - Cell Biology 2021Quote: ... The homogenized solution was centrifuged at 75,000 rpm for 15 min (Beckman, TL100.3). The supernatant was loaded onto a StrepTactin Sepharose column with a volume of 1 mL ...
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bioRxiv - Genomics 2019Quote: ... and using 15 cycles of PCR followed by an Ampure XP bead (Beckman) clean-up.
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bioRxiv - Cell Biology 2023Quote: ... continuous 15-45 % (w/v) sucrose gradients were prepared in SW41 tubes (Beckman) in Polysome Gradient Buffer (10 mM Hepes-KOH ...
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bioRxiv - Molecular Biology 2023Quote: ... Lysates were loaded on 15-50% sucrose density gradients and centrifuged (Beckman ultracentrifuge) for 1 hour and 30 minutes in a SW41Ti swinging bucket rotor at 39000 rpm at 4 °C ...
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bioRxiv - Cell Biology 2020Quote: ... The 1 ml concentrates were overlaid on top of the gradient, and centrifuged at 100 000 g (28 000 rpm, k-factor 266) in an Optima L-90K ultracentrifuge (Beckman Coulter) using an SW41 Ti rotor for 18 h at 4 °C with slow deacceleration ...
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bioRxiv - Biophysics 2022Quote: ... EVs were separated from protein aggregates and free reagents by bottom-up density gradient centrifugation in sucrose for 17.30 h at 192,000 g and 4°C using a SW40 rotor (k-factor 144.5; Beckman Coulter, Fullerton, California, USA). Twelve fractions of 1 mL were then collected from the top of the gradient and respective densities were determined by refractometry using an Atago Illuminator (Japan) ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were ultracentrifuged at 180,000 × g (38,000 rpm) in a SW 41 Ti Swinging-Bucket Rotor (Beckman Coulter, k-factor 145) for 2 hours at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... with known Equivalent number of Reference Fluorophores (ERF) values for C30/FITC/APC (as determined on a Beckman Coulter CytoFLEX) were used in the standardization of the fluorescent detection channels Ch01,Ch02 & Ch05 ...
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bioRxiv - Microbiology 2021Quote: The following fluorochrome-conjugated Abs were used for flow cytometry analysis: HIV-p24 FITC (KC57) (Beckman Coulter, Fullerton, CA, USA), HIV-p24 PE (KC57 ...
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bioRxiv - Immunology 2020Quote: ... PE-CD11b and FITC-CD3 (all Ebioscience) for 20 minutes and washed before sorting using a Moflo Astrios (Beckman Coulter) or FACSAria IIu (BD BioSciences) ...
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bioRxiv - Microbiology 2023Quote: ... PBS-washed cells were PFA-fixed for 90 minutes and immunostained with the following antibodies diluted in 0.1% Triton X-100 in PBS: anti-HIV-1-core-antigen-FITC or -PE (#6604665 or #6604667; Beckman Coulter), rabbit-anti-human-MX1/2 (#sc-166412 ...
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bioRxiv - Neuroscience 2020Quote: ... We purified the transcription products using RNAse-free SPRI beads (Agencourt RNAclean XP, Beckman-Coulter A63987) and eluted them in Ambion nuclease-free water (Life Technologies ...
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bioRxiv - Immunology 2022Quote: ... Reverse transcription products were subject to preamplification and purification with SPRIselect Reagent Kit (Beckman Coulter, B23318). BCR sequences (paired V(D)J ...
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bioRxiv - Cancer Biology 2021Quote: ... Purified cDNA was amplified for 15 cycles and cleaned up using SPRIselect beads (Beckman). Samples were quantified on an Invitrogen Qubit 4 Fluorometer ...
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bioRxiv - Cell Biology 2020Quote: ... for 15 min at 37°C before analysis using CytoFLEX (Beckman Coulter Life Science). Treatment with 10 µM valinomycin for 30 min was included prior to JC-1 incubation as a depolarising control ...
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bioRxiv - Genomics 2020Quote: ... Purified cDNA was amplified for 15 cycles before bead cleanup using SPRIselect beads (Beckman). Samples were quantified using an Invitrogen Qubit 4 Fluorometer ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were harvested by centrifugation for 15 minutes in a JLA-9.1000 rotor (Beckman) at 5,180 x g and the pellets were stored at –20 °C.
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bioRxiv - Cell Biology 2019Quote: ... for 15 minutes at room temperature and analyzed with flow cytometry (Beckman Coulter, Cytoflex). Alternatively ...
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bioRxiv - Molecular Biology 2022Quote: ... After centrifugation at 6000 g for 15 min at 4 °C (JA14 rotor-Beckman), the cell pellet is resuspended with cold lysis buffer (1mg/ml lysozyme ...
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bioRxiv - Genetics 2021Quote: ... 15-60% sucrose gradients were prepared in 89 mm polypropylene centrifuge tubes (Beckman Coulter) using standard settings on a Foxy Jr ...
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bioRxiv - Immunology 2019Quote: ... Lymphocyte counts (4-15 μm) were determined with a Z2 Coulter Counter (Beckman Coulter). For surface staining ...
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bioRxiv - Microbiology 2021Quote: ... and centrifuged each time at 4000 × g for 15 min (Microfuge 20R, Beckman Coulter) followed by resuspension in PBS.
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bioRxiv - Genomics 2023Quote: ... the cDNA was cleaned up and eluted into 15 µL using AMPure beads (Beckman) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: A 15-45% continuous sucrose gradient was made in Thinwall polyallomer tubes (Beckman Coulter) from 15% and 45% sucrose (MP Biomedicals ...
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bioRxiv - Biophysics 2022Quote: ... The tubes were filled with PBS to reach the final volume and samples were ultracentrifuged at 120,000 g for 60 minutes at 4°C (70.1 Ti rotor, k-factor 36, Beckman Coulter, Brea, CA, USA). Finally ...
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bioRxiv - Cell Biology 2021Quote: ... Virus was concentrated from cell-free culture supernatant by high-speed ultracentrifugation through a 30% sucrose cushion at 80,000xg for 16 hrs in a SW32 rotor (k-factor 321) (Beckman Coulter, Brea, CA).
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bioRxiv - Neuroscience 2023Quote: ... the samples were concentrated to a final volume of 240 µl through ultracentrifugation at 42,300 rpm using a Beckman TLA-55 rotor (RCFavg: 80,160; RCFmax: 110,220; k-factor: 110 and the Optima MAX-XP ultracentrifuge (both Beckman Coulter, Brea, CA, USA) and equal sample volumes were immediately used for the EV Neuro panel A and B (115±25 µl ...
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bioRxiv - Cell Biology 2024Quote: ... while the supernatant was further centrifuged at 118.000 × g (38,800 rpm) (Type Ti 45 fixed-angle rotor, Beckman Coulter, k-factor 178) for 2.5 hours at 4°C to isolate small EVs that were resuspended in PBS and stored at –80°C.
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bioRxiv - Cancer Biology 2020Quote: ... the pellets were resuspended in 500 μl PBS and analyzed by flow cytometry using the FITC channel of a FC-500 flow cytometer (Beckman Coulter). All manipulations were performed in 1.7 ml Eppendorf tubes ...
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bioRxiv - Cell Biology 2019Quote: ... apoptotic and necrotic cells with YO-PRO-1 / PI in parallel with a Annexin V / PI staining realized with the Annnexin-V-FITC when needed (Immunotech, Beckman-Coulter). All samples were measured and analyzed on a FACS Calibur 4C as previously described (23 ...
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bioRxiv - Cell Biology 2019Quote: ... apoptotic and necrotic cells with YO-PRO-1 / PI in parallel with a Annexin-V / PI staining realized with the Annnexin-V-FITC when needed (Immunotech, Beckman-Coulter). All samples were measured and analyzed on a FACS Calibur 4C as previously described (73 ...
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bioRxiv - Cell Biology 2019Quote: ... Comparative analysis of MLVsfGFP and MLVnoGFP viruses on two CytoFLEX S flow cytometers from two different institutions using 7µm PS FITC-MESF beads (top panel: uOttawa, bottom panel: Beckman Coulter (Inst1)) ...
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bioRxiv - Developmental Biology 2019Quote: Detection of apoptotic TCam-2 cells was performed 48 h after transfection of TCam-2 cells with control and KIF18A siRNA constructs using an Annexin V-FITC Apoptosis Detection Kit (Beckman Coulter), according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... stained the cells by a Annexin V-FITC/PI Apoptosis Detection Kit (Vazyme, A211-01) and detected cells distribution using a Beckman Coulter Gallios (Beckman Coulter). The result was analyzed by Kaluza Analysis 2.1 (Beckman Coulter).
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bioRxiv - Microbiology 2023Quote: ... followed by flow cytometry analysis of CAp24 antigen expression on fixed and permeabilized cells labelled with KC57-fluorescein isothiocyanate (FITC) (Beckman Coulter).
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bioRxiv - Microbiology 2024Quote: Intracellular HIV-1 p24 staining was performed on fixed U1 and ACH-2 cells with KC57-FITC (Beckman Coulter, Cat#6604665). After allowing at least 30 minutes for fixation ...
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bioRxiv - Biochemistry 2021Quote: ... The bacterial culture was centrifuged at 4,000 × g for 15 min at 4°C (Beckman) and the cell pellet was resuspended in 30-40 mL of lysis buffer (50 mM HEPES pH 8.0 ...
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bioRxiv - Immunology 2021Quote: ... 5000 rpm for 15 min using the Avanti® J-26xp centrifuge (Beckman Coulter®) and resuspended in lysis buffer composed of 50 mM Tris/HCl pH 8 ...